##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938113_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1994970 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.46169365955378 33.0 31.0 34.0 31.0 34.0 2 32.67940269778493 34.0 31.0 34.0 31.0 34.0 3 32.74889296580901 34.0 31.0 34.0 31.0 34.0 4 36.203393534739874 37.0 35.0 37.0 35.0 37.0 5 36.174767540364016 37.0 35.0 37.0 35.0 37.0 6 36.21022421389795 37.0 37.0 37.0 35.0 37.0 7 36.18086487516103 37.0 37.0 37.0 35.0 37.0 8 36.17531591953764 37.0 37.0 37.0 35.0 37.0 9 37.882229306706364 39.0 38.0 39.0 35.0 39.0 10-11 37.86411123976802 39.0 38.0 39.0 35.0 39.0 12-13 37.819335127846536 39.0 38.0 39.0 35.0 39.0 14-15 39.10864023017891 40.0 38.5 41.0 36.0 41.0 16-17 39.05925602891271 40.0 38.0 41.0 36.0 41.0 18-19 39.10765274665785 40.0 38.5 41.0 36.0 41.0 20-21 39.051766442603146 40.0 39.0 41.0 35.5 41.0 22-23 38.90652315573668 40.0 38.0 41.0 35.0 41.0 24-25 38.88766773435189 40.0 38.0 41.0 35.0 41.0 26-27 38.8407156498594 40.0 38.0 41.0 35.0 41.0 28-29 38.78481856870028 40.0 38.0 41.0 35.0 41.0 30-31 38.62233542359033 40.0 38.0 41.0 35.0 41.0 32-33 38.47008827200409 40.0 38.0 41.0 34.0 41.0 34-35 38.30542364045574 40.0 38.0 41.0 34.0 41.0 36-37 38.193936249667914 40.0 38.0 41.0 34.0 41.0 38-39 38.18244961077109 40.0 38.0 41.0 34.0 41.0 40-41 38.12950670937408 40.0 38.0 41.0 34.0 41.0 42-43 38.017322816884466 40.0 38.0 41.0 33.5 41.0 44-45 37.82527456553231 40.0 38.0 41.0 33.0 41.0 46-47 37.515302736382 40.0 38.0 41.0 33.0 41.0 48-49 37.209780848834825 40.0 37.0 41.0 32.0 41.0 50-51 36.95564544830248 40.0 37.0 41.0 31.0 41.0 52-53 36.55197221010842 40.0 36.0 41.0 30.0 41.0 54-55 36.381254855962744 39.0 36.0 41.0 30.0 41.0 56-57 36.20909712928014 39.0 36.0 41.0 30.0 41.0 58-59 35.94681724537212 39.0 35.5 40.5 28.5 41.0 60-61 35.64080211732507 39.0 35.0 40.0 28.0 41.0 62-63 35.301610299904254 38.0 35.0 40.0 28.0 41.0 64-65 34.93854594304676 38.0 34.0 40.0 27.0 41.0 66-67 34.547303718852916 37.5 34.0 40.0 26.0 41.0 68-69 34.12005468753917 37.0 34.0 39.5 26.0 41.0 70-71 33.61481250344617 36.5 33.0 39.0 25.5 40.0 72-73 33.17087876008161 36.0 33.0 39.0 24.5 40.0 74-75 32.70842067800518 35.0 32.0 38.0 22.5 40.0 76-77 31.293325964801475 34.0 30.5 36.0 21.5 39.0 78-79 32.01587166724312 35.0 32.0 37.0 23.0 39.0 80-81 31.837699564404478 35.0 32.0 36.5 22.0 39.0 82-83 31.439112868865195 35.0 32.0 36.0 20.5 37.5 84-85 31.016772683298495 34.5 31.0 35.5 20.0 37.0 86-87 30.52814002215572 34.0 31.0 35.0 17.5 36.5 88-89 30.031565888208846 34.0 30.5 35.0 11.5 36.0 90-91 29.654762978891913 34.0 30.0 35.0 7.0 36.0 92-93 29.25520559206404 34.0 30.0 35.0 2.0 35.0 94-95 28.844993157791848 34.0 29.0 35.0 2.0 35.0 96-97 28.2590833446117 34.0 29.0 35.0 2.0 35.0 98-99 27.55973072276776 34.0 28.0 35.0 2.0 35.0 100-101 25.13770432638085 31.0 22.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0958398850172344 1101 2 0.18429818023024325 1101 3 0.1856994138547563 1101 4 0.10023194769203769 1101 5 0.10355653146478261 1101 6 -0.006799499414185561 1101 7 0.05880173459964766 1101 8 0.07472162797227355 1101 9 0.10335922583281132 1101 10-11 0.09340200606427373 1101 12-13 0.15332158231433368 1101 14-15 0.13894704910428146 1101 16-17 0.24995668678565863 1101 18-19 0.18498634933352065 1101 20-21 0.2145494981337137 1101 22-23 0.20426333788893913 1101 24-25 0.15126705934861917 1101 26-27 0.23882098599305834 1101 28-29 0.17751622388952626 1101 30-31 0.11242996663865767 1101 32-33 0.20452607278551227 1101 34-35 0.11080650082071486 1101 36-37 0.18765707330654635 1101 38-39 0.2641466035959681 1101 40-41 0.37005940406406523 1101 42-43 0.2573129980056166 1101 44-45 0.18969022571817362 1101 46-47 0.2200025015994882 1101 48-49 0.37204809791866467 1101 50-51 0.3811364857660138 1101 52-53 0.24719944925558934 1101 54-55 0.34841387249187505 1101 56-57 0.4514784041497748 1101 58-59 0.32669748589926684 1101 60-61 0.41337976107293883 1101 62-63 0.5179851630552434 1101 64-65 0.3953459995497539 1101 66-67 0.37042416796488453 1101 68-69 0.39668930599906815 1101 70-71 0.2754922494535563 1101 72-73 0.2038067656977134 1101 74-75 0.37891139461236634 1101 76-77 0.3316280643393661 1101 78-79 0.4302095985929455 1101 80-81 0.5268942253094551 1101 82-83 0.5786161904298872 1101 84-85 0.48622614915606377 1101 86-87 0.5711727193960847 1101 88-89 0.45885224783800993 1101 90-91 0.37426822570824214 1101 92-93 0.5522231513343066 1101 94-95 0.4556255654799486 1101 96-97 0.6687850232201313 1101 98-99 0.8020529947291628 1101 100-101 0.9410705596120863 1102 1 0.19630272005453975 1102 2 0.16522292924009463 1102 3 0.16697647013180728 1102 4 0.12629117215551844 1102 5 0.11303659041292491 1102 6 0.030630269398557175 1102 7 0.005142804876882678 1102 8 0.09752949386525245 1102 9 0.01620807910719435 1102 10-11 0.08752100763008741 1102 12-13 0.09589434642964534 1102 14-15 0.23171553947915413 1102 16-17 0.23580567821975507 1102 18-19 0.21638429535911996 1102 20-21 0.08843731244706277 1102 22-23 0.12903882835591673 1102 24-25 0.13443920635055662 1102 26-27 -0.03362305932333243 1102 28-29 0.01538464978987264 1102 30-31 0.022187496342993995 1102 32-33 0.03523437157890896 1102 34-35 0.10613900519577157 1102 36-37 0.12560494104165087 1102 38-39 0.05590422540678475 1102 40-41 0.061126227311739 1102 42-43 -0.10955515157530016 1102 44-45 -0.02253230352563662 1102 46-47 0.013179714457557168 1102 48-49 -0.038632799198403234 1102 50-51 0.018289935153511294 1102 52-53 0.04589729396407449 1102 54-55 0.1365931577456294 1102 56-57 0.2749160480661459 1102 58-59 0.17891840327975927 1102 60-61 -0.019315734920425598 1102 62-63 0.008596059251246402 1102 64-65 0.20436667745341452 1102 66-67 0.019497346559340656 1102 68-69 -0.014813641408132128 1102 70-71 -0.031715082221857926 1102 72-73 0.07711776321083619 1102 74-75 0.10167896392249531 1102 76-77 0.1659711149058225 1102 78-79 0.15826062558013376 1102 80-81 0.20926044065438987 1102 82-83 0.31215446067028196 1102 84-85 0.1876307729011124 1102 86-87 0.21488069581682723 1102 88-89 -0.01658410658770748 1102 90-91 0.06255688740115772 1102 92-93 0.14807720862453166 1102 94-95 0.24283082475967177 1102 96-97 0.343309523542505 1102 98-99 0.3206757726604508 1102 100-101 0.44139984026051593 1103 1 0.09282311012545819 1103 2 0.1370646845592418 1103 3 0.10760147013181154 1103 4 0.06194055158814393 1103 5 0.09540582800158148 1103 6 0.11824108500140085 1103 7 0.08654794672085586 1103 8 0.05426708251773249 1103 9 0.1587501890362688 1103 10-11 0.07393501472228792 1103 12-13 0.13650272408921893 1103 14-15 0.16982502529476307 1103 16-17 0.1274391002055708 1103 18-19 0.14706359500451072 1103 20-21 0.21900025570947435 1103 22-23 0.10695881949067143 1103 24-25 0.13320361237892797 1103 26-27 0.09393124032206401 1103 28-29 0.14551541220121322 1103 30-31 0.03985150343520161 1103 32-33 0.20212821023139327 1103 34-35 0.1566432161886837 1103 36-37 0.043917219410445796 1103 38-39 0.03797980164791426 1103 40-41 0.13586026986492783 1103 42-43 0.12542379346016475 1103 44-45 -0.091143784022087 1103 46-47 -0.07719040965591972 1103 48-49 -0.07446502437571212 1103 50-51 -0.0806296127188304 1103 52-53 -0.12721884078769818 1103 54-55 -0.16376145218344362 1103 56-57 -0.09760168243030165 1103 58-59 -0.25297986799683514 1103 60-61 -0.09003271187079065 1103 62-63 0.055437814570396426 1103 64-65 -0.07260584027708461 1103 66-67 -0.07751617294420754 1103 68-69 0.0010994791592437991 1103 70-71 -0.05861556981051308 1103 72-73 -0.20167213040618037 1103 74-75 -0.1532855750846025 1103 76-77 -0.1551447982147458 1103 78-79 -0.12856339924255522 1103 80-81 -0.05961145650872979 1103 82-83 -0.0213229329467417 1103 84-85 0.16197696084437396 1103 86-87 0.21557883411470158 1103 88-89 0.17071642532718556 1103 90-91 0.1833625168337818 1103 92-93 0.10031568380183487 1103 94-95 -0.011169618502737677 1103 96-97 0.12444316715952652 1103 98-99 0.3891099481923668 1103 100-101 0.6127595477073271 1104 1 0.10529749965547808 1104 2 0.10238784687735603 1104 3 0.1401199723897122 1104 4 0.05038160569418437 1104 5 0.05860645728741076 1104 6 0.04234680920276901 1104 7 0.055941344446225116 1104 8 0.08836977527571577 1104 9 0.07511345003309344 1104 10-11 0.1231442261288791 1104 12-13 0.09684007669835637 1104 14-15 0.18526840002297007 1104 16-17 0.06291038511943725 1104 18-19 0.20434441642113654 1104 20-21 0.12162768086418652 1104 22-23 0.14868284838345858 1104 24-25 0.214531097117181 1104 26-27 0.24585738286262426 1104 28-29 0.19690334287523825 1104 30-31 0.1438117183540797 1104 32-33 0.13683732233208445 1104 34-35 0.22198689301590946 1104 36-37 0.2153256123273195 1104 38-39 0.23481939125320395 1104 40-41 0.2845933355949839 1104 42-43 0.37560581072893484 1104 44-45 0.36032084739620274 1104 46-47 0.30506754526965807 1104 48-49 0.19608672445352937 1104 50-51 0.337719374360951 1104 52-53 0.3961823341579489 1104 54-55 0.27666545867121073 1104 56-57 0.4320918281902024 1104 58-59 0.42943083508895796 1104 60-61 0.395225718677807 1104 62-63 0.31224993825328795 1104 64-65 0.17868984043875713 1104 66-67 0.3034216667865195 1104 68-69 0.44857083724588165 1104 70-71 0.4499039620928116 1104 72-73 0.3940049267940182 1104 74-75 0.37878500528001524 1104 76-77 0.40420106334237005 1104 78-79 0.40052303870693606 1104 80-81 0.3695471088868203 1104 82-83 0.29245105726901244 1104 84-85 0.21745577210043976 1104 86-87 0.32851551876095897 1104 88-89 0.29906721559311933 1104 90-91 0.3854870098510119 1104 92-93 0.5441216087056695 1104 94-95 0.5502952481137555 1104 96-97 0.5494905987952485 1104 98-99 0.6042994395370727 1104 100-101 0.7891131133119416 1105 1 0.03892059323634811 1105 2 0.061526344223111096 1105 3 0.04820484383423462 1105 4 0.02572139480778901 1105 5 -0.025059085905162704 1105 6 0.07413528091505839 1105 7 0.03948036969004676 1105 8 0.07451753564015462 1105 9 -0.010900649240213056 1105 10-11 0.04973467282081856 1105 12-13 -0.013788844744198059 1105 14-15 0.16995504053614496 1105 16-17 0.06050751139357402 1105 18-19 0.0743589432908891 1105 20-21 0.17069586042195084 1105 22-23 0.2171408150830274 1105 24-25 0.15697696363118752 1105 26-27 0.19385233008149783 1105 28-29 0.08250281745743138 1105 30-31 0.13660086494450496 1105 32-33 0.08476319437211544 1105 34-35 0.1763866098696809 1105 36-37 0.17345861317383537 1105 38-39 0.061226398080613365 1105 40-41 0.0783590340764988 1105 42-43 0.001317551698754471 1105 44-45 -0.1083079842610104 1105 46-47 -0.03991001955870388 1105 48-49 0.1510348262998562 1105 50-51 0.01362049541265975 1105 52-53 8.309820826255532E-4 1105 54-55 0.07469422420475524 1105 56-57 -0.01230550513586337 1105 58-59 -0.08382862293999693 1105 60-61 0.04808350888222179 1105 62-63 0.13020224240035816 1105 64-65 0.21436946455073524 1105 66-67 0.07809186071043683 1105 68-69 0.16205989273644406 1105 70-71 0.24751381208930212 1105 72-73 0.3151176001919467 1105 74-75 0.12966878312927577 1105 76-77 0.23993817982941223 1105 78-79 0.21420595114465613 1105 80-81 0.1122020115255431 1105 82-83 0.22782360066670293 1105 84-85 0.35971418073232897 1105 86-87 0.27128431483640014 1105 88-89 0.17227197401725292 1105 90-91 0.05121721385965117 1105 92-93 0.03082060635909656 1105 94-95 -0.020260644619064294 1105 96-97 0.13161962230040558 1105 98-99 0.3184796188547523 1105 100-101 0.36471943092531944 1106 1 -0.19104929584081987 1106 2 -0.11341561699717317 1106 3 -0.06882119523783814 1106 4 -0.04181877072054618 1106 5 -0.06310381856132352 1106 6 -0.06570427816332369 1106 7 -0.0059901531494404026 1106 8 -0.11875496676891117 1106 9 -0.13836597503635772 1106 10-11 -0.10789458968756804 1106 12-13 -0.225817922797944 1106 14-15 -0.4369863398154905 1106 16-17 -0.2644768368891093 1106 18-19 -0.2423322788606015 1106 20-21 -0.07878265609337376 1106 22-23 -0.0897510135184092 1106 24-25 -0.17078370117490493 1106 26-27 -0.06383768801775602 1106 28-29 -0.11090684785066429 1106 30-31 -0.11520280914587033 1106 32-33 -0.15478549761555627 1106 34-35 -0.1559915519695565 1106 36-37 -0.1675360474507812 1106 38-39 -0.19984749245065103 1106 40-41 -0.061075527800440454 1106 42-43 -0.2207408871494394 1106 44-45 -0.19350982649939397 1106 46-47 -0.1537204842343911 1106 48-49 -0.10425081393471913 1106 50-51 -0.018418401629340053 1106 52-53 -0.12131336089146316 1106 54-55 -0.4393775351925271 1106 56-57 -0.19776380953796036 1106 58-59 -0.1115857775299176 1106 60-61 -0.15230066744796034 1106 62-63 -0.12086933523504939 1106 64-65 -0.3442052241940772 1106 66-67 -0.33316326276261776 1106 68-69 -0.5319663444464666 1106 70-71 -0.48597350593242794 1106 72-73 -0.3092718385773878 1106 74-75 -0.3161422547214343 1106 76-77 -0.3940907693561222 1106 78-79 -0.2179987915901549 1106 80-81 -0.4891489889341507 1106 82-83 -0.585616464075148 1106 84-85 -0.38383002683396583 1106 86-87 -0.34766897716934864 1106 88-89 -0.20825974198798747 1106 90-91 -0.41226511773171737 1106 92-93 -0.22083785056910799 1106 94-95 -0.2825075724586412 1106 96-97 -0.28479503809339235 1106 98-99 -0.25810247885043225 1106 100-101 -0.1814859532654367 1107 1 0.11118620536355195 1107 2 0.09124623217829253 1107 3 0.10673837489371607 1107 4 0.03888995635004733 1107 5 0.036759994668244644 1107 6 0.06213989452520963 1107 7 0.06973247053038278 1107 8 0.0642670825177305 1107 9 0.16156268903627335 1107 10-11 0.07979810996038594 1107 12-13 0.10485837885111948 1107 14-15 0.09882800148523785 1107 16-17 0.17682898115794643 1107 18-19 0.12518115452832035 1107 20-21 0.15045858904280607 1107 22-23 0.1374126885382907 1107 24-25 0.10060688618845148 1107 26-27 0.08874522841730226 1107 28-29 0.11106600743931239 1107 30-31 0.1835122177209172 1107 32-33 0.07816243642187004 1107 34-35 0.1784363709505854 1107 36-37 0.14685620750567807 1107 38-39 0.2505318849812497 1107 40-41 0.2534793174839791 1107 42-43 0.1901484958411146 1107 44-45 0.10911663264457871 1107 46-47 0.08850899510598964 1107 48-49 0.09880134467191226 1107 50-51 0.15527068490021634 1107 52-53 0.17060854016468596 1107 54-55 0.24373854781655524 1107 56-57 0.26370784137922243 1107 58-59 0.25768233438411414 1107 60-61 0.31341222860540796 1107 62-63 0.4256907907608749 1107 64-65 0.42016201686577404 1107 66-67 0.3310626961034089 1107 68-69 0.3248494791592407 1107 70-71 0.4822996087609184 1107 72-73 0.3627028695938179 1107 74-75 0.30004775824873064 1107 76-77 0.2616929994042998 1107 78-79 0.2867788626622101 1107 80-81 0.37829032920555505 1107 82-83 0.4648824241961158 1107 84-85 0.5982716037015159 1107 86-87 0.5056532388766044 1107 88-89 0.396460472946238 1107 90-91 0.3895604335004492 1107 92-93 0.571238302849455 1107 94-95 0.5310476434020259 1107 96-97 0.6362958457309524 1107 98-99 0.6702781029542706 1107 100-101 0.8453562738978029 1108 1 -0.0026743470265486735 1108 2 0.08008561800470204 1108 3 0.06321625869500025 1108 4 0.007067343595196007 1108 5 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-0.6374112176362274 2316 100-101 -0.8051477134426506 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 10.0 4 22.0 5 46.0 6 65.0 7 132.0 8 364.0 9 689.0 10 1140.0 11 2049.0 12 3068.0 13 5042.0 14 6278.0 15 8467.0 16 11840.0 17 16767.0 18 17464.0 19 11759.0 20 9253.0 21 8923.0 22 9208.0 23 10322.0 24 11956.0 25 14030.0 26 16630.0 27 20158.0 28 24576.0 29 30405.0 30 37504.0 31 46252.0 32 58322.0 33 75427.0 34 103495.0 35 156040.0 36 248097.0 37 461094.0 38 508942.0 39 59124.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.16469420592791 19.92446001694261 15.133059645007194 22.777786132122287 2 14.501410761089065 21.414591300406887 41.42965145076774 22.654346487736305 3 19.05114717841082 25.557547907086796 30.478015651294253 24.91328926320813 4 11.830403464713756 18.103530378902942 39.25397374396608 30.81209241241723 5 12.652521090542715 38.704792553271474 35.14488939683304 13.497796959352772 6 29.637638661233 37.8223231427039 18.850308525942747 13.689729670120352 7 26.846468869206053 33.78090898610004 21.418216815290457 17.95440532940345 8 24.30447157283512 36.24682825402841 20.75537429860679 18.69332587452969 9 25.533944472180952 17.378759716967323 21.37366889828588 35.71362691256584 10-11 23.500528830007468 27.473946976646264 28.56035429104197 20.465169902304297 12-13 25.266144353047913 25.046341548995727 28.33152879491922 21.35598530303714 14-15 21.930204464227533 26.753459951778723 27.606254730647578 23.710080853346167 16-17 20.359168578350985 29.776259625151972 28.676893895818406 21.18767790067863 18-19 19.904610094387383 29.03509827215447 30.81780678406191 20.24248484939623 20-21 21.649272921397316 27.824603878755067 30.328676621703583 20.19744657814403 22-23 20.556675037719867 28.46762106698346 29.665609006651728 21.310094888644944 24-25 20.328655630938407 28.104312395794377 30.775685839665456 20.791346133601756 26-27 20.87155043353659 28.187198361916565 30.36776657712158 20.573484627425263 28-29 20.132763934486217 28.37712556507756 30.772249901564912 20.71786059887131 30-31 20.652691519170716 28.644616209767566 30.620610836253174 20.082081434808543 32-33 20.198515407713995 28.58510590571182 30.34288683198172 20.873491854592462 34-35 20.658057053871957 28.436615011830966 30.3379177344704 20.56741019982668 36-37 20.76774562072546 28.341160456057622 30.33834875740615 20.55274516581077 38-39 20.877290088008767 28.138163838493835 30.621425928544138 20.36312014495326 40-41 21.054523495646198 27.938137068151047 30.78181452200164 20.225524914201117 42-43 20.314159799998095 28.414673089426863 30.583056485899956 20.68811062467509 44-45 20.72446993397641 28.785077695896472 29.93792148250558 20.552530887621543 46-47 20.52044932037774 28.472680145074975 29.314949083155952 21.691921451391337 48-49 20.843396141295358 28.25546248815772 29.764307232690218 21.13683413785671 50-51 21.393279096928776 28.689504102818585 29.237281763635544 20.679935036617092 52-53 20.78607698361379 28.64286179742051 29.81929552825356 20.75176569071214 54-55 20.632691218414312 28.46586665463641 29.897943327468585 21.003498799480695 56-57 20.975127445525494 29.076527466578444 28.961989403349424 20.986355684546634 58-59 20.70903321854464 28.915748106487815 29.181040316395734 21.194178358571808 60-61 20.950640861767344 28.495566349368662 29.236028611959075 21.317764176904916 62-63 21.4335553918104 28.26644009684356 29.26976345508955 21.030241056256486 64-65 21.190569281743585 28.344912454823884 29.347684426332226 21.11683383710031 66-67 20.560860564319263 28.543712436778495 29.587763224509644 21.307663774392598 68-69 20.793071557646375 28.84709756812776 29.256277679057025 21.103553195168836 70-71 20.671689298585942 28.45581637819115 28.996776893888125 21.875717429334777 72-73 20.63026010416197 28.2397229031013 28.994796914239313 22.135220078497422 74-75 20.931417515050352 28.184759670571484 28.894970851692005 21.988851962686155 76-77 20.927382366652132 27.89194824984837 29.383674942480337 21.796994441019162 78-79 20.5939688316115 27.862273618149647 29.505857230935806 22.03790031930305 80-81 20.473716396737796 28.570955954224875 29.093670581512505 21.861657067524824 82-83 20.635333180615838 28.457158983422797 29.023734203388624 21.883773632572737 84-85 20.88605723079545 27.894160373551625 28.972682909712493 22.24709948594043 86-87 20.707095643604507 28.015926105916222 29.200534245363386 22.076444005115885 88-89 21.343093888449868 27.51269004999327 29.205999994486127 21.938216067070734 90-91 20.769257374698178 27.81607979696867 29.464656737994638 21.950006090338515 92-93 20.41014785483544 28.05254331720856 29.45454891781228 22.08275991014372 94-95 20.33406517391239 28.37624124673554 28.988330651588747 22.301362927763325 96-97 20.348125535722343 27.987037399058636 28.81857872549462 22.846258339724407 98-99 20.733795495671615 27.606054226379346 28.943392632470665 22.716757645478378 100-101 20.998235562439536 27.652044892905657 29.01910805676276 22.33061148789205 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2445.0 1 1388.5 2 545.0 3 782.0 4 554.0 5 481.0 6 1268.5 7 2587.0 8 3452.0 9 3516.0 10 2827.0 11 2081.5 12 1959.5 13 1993.5 14 2032.0 15 2060.0 16 2099.0 17 2308.0 18 2632.0 19 3060.0 20 3673.5 21 4409.5 22 5334.5 23 6605.5 24 8322.0 25 10394.5 26 12805.5 27 15829.0 28 19270.5 29 23503.0 30 28196.0 31 33038.5 32 38480.5 33 44195.5 34 50261.5 35 56150.5 36 61423.0 37 65779.0 38 68555.5 39 70500.5 40 71995.5 41 73282.0 42 74755.5 43 76180.0 44 77907.0 45 79784.0 46 81791.5 47 83771.5 48 102495.5 49 101133.5 50 78365.0 51 70914.0 52 63255.5 53 55660.0 54 48709.0 55 42000.0 56 35406.0 57 28975.0 58 22668.5 59 17530.5 60 12976.5 61 9204.0 62 6550.5 63 4472.0 64 2888.5 65 1838.0 66 1281.5 67 998.0 68 766.5 69 542.0 70 367.5 71 258.0 72 179.5 73 143.5 74 122.5 75 75.5 76 52.0 77 34.0 78 18.5 79 15.0 80 12.0 81 7.0 82 4.5 83 3.5 84 1.0 85 0.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.015138072251713057 3 0.005965001979979649 4 0.0 5 0.0 6 0.0 7 0.0 8 6.015128047038302E-4 9 3.007564023519151E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 7.518910058797877E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005814623778803691 26-27 0.022255973774041714 28-29 2.5063033529326258E-5 30-31 0.0 32-33 5.764497711745038E-4 34-35 0.02087750692992877 36-37 0.004536409068808052 38-39 0.019849922555226395 40-41 0.016767169431119264 42-43 0.009473826674085323 44-45 0.003859707163516243 46-47 7.518910058797877E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.5063033529326256E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 5.0126067058652516E-5 84-85 0.0032581943588124132 86-87 3.258194358812413E-4 88-89 7.518910058797877E-5 90-91 3.5088246941056755E-4 92-93 3.258194358812413E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1994970.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.72089232959142 #Duplication Level Percentage of deduplicated Percentage of total 1 76.16500891823208 50.056323503974994 2 14.946542985310376 19.646002844743865 3 4.704566589087317 9.275649427764021 4 1.9796180310215505 5.204090538819404 5 0.9275321085532593 3.0479118919233827 6 0.471322693637226 1.8585448800615079 7 0.2750897658881228 1.265540141943408 8 0.1611763036587405 0.8474120399070093 9 0.0973266781815963 0.5756756523812536 >10 0.2513632072162663 2.430889950357354 >50 0.00931865682966994 0.42346087695049084 >100 0.008313740714162392 1.0810585349738406 >500 0.0011433736498523024 0.5201936555823743 >1k 0.0012195985598423796 1.5207548923838028 >5k 3.8112454995074363E-4 1.7283514359829226 >10k+ 7.622490999014873E-5 0.5181397322504092 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10343 0.518453911587643 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8338 0.4179511471350446 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 8169 0.40947984180213237 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6636 0.33263658100121807 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6027 0.30210980616249866 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 5331 0.26722206348967653 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3204 0.16060391885592265 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2801 0.14040311383128568 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2768 0.13874895361835016 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2696 0.13513987679012715 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2453 0.12295924249487461 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2036 0.10205667253141651 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2019 0.10120452939141943 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 5.012606705865251E-5 0.0 7 0.0 0.0 0.0 5.012606705865251E-5 0.0 8 0.0 0.0 0.0 5.012606705865251E-5 0.0 9 0.0 0.0 0.0 5.012606705865251E-5 0.0 10-11 0.0 0.0 0.0 5.012606705865251E-5 0.0 12-13 0.0 0.0 0.0 5.012606705865251E-5 0.0 14-15 0.0 0.0 0.0 5.012606705865251E-5 0.0 16-17 0.0 0.0 0.0 7.518910058797876E-5 0.0 18-19 5.012606705865251E-5 0.0 0.0 1.5037820117595751E-4 0.0 20-21 5.012606705865251E-5 0.0 0.0 3.2581943588124134E-4 0.0 22-23 5.012606705865251E-5 0.0 0.0 6.265758382331564E-4 0.0 24-25 5.012606705865251E-5 0.0 0.0 0.001152899542349008 0.0 26-27 5.012606705865251E-5 0.0 0.0 0.002481240319403299 0.0 28-29 5.012606705865251E-5 0.0 0.0 0.005739434678215713 0.0 30-31 5.012606705865251E-5 0.0 0.0 0.017895005939938945 0.0 32-33 5.012606705865251E-5 0.0 0.0 0.04260715699985464 0.0 34-35 5.012606705865251E-5 0.0 0.0 0.08378572108853767 0.0 36-37 5.012606705865251E-5 0.0 0.0 0.1492002386000792 0.0 38-39 5.012606705865251E-5 0.0 0.0 0.2376978099921302 0.0 40-41 5.012606705865251E-5 0.0 0.0 0.3295287648435816 0.0 42-43 5.012606705865251E-5 0.0 0.0 0.41920429881151094 0.0 44-45 5.012606705865251E-5 0.0 0.0 0.5311107435199527 0.0 46-47 5.012606705865251E-5 0.0 0.0 0.6601853661959829 0.0 48-49 5.012606705865251E-5 0.0 0.0 0.801315307999619 0.0 50-51 5.012606705865251E-5 0.0 0.0 0.9388612360085615 0.0 52-53 5.012606705865251E-5 0.0 0.0 1.0717454397810493 0.0 54-55 5.012606705865251E-5 0.0 0.0 1.2184895010952546 0.0 56-57 5.012606705865251E-5 0.0 0.0 1.378466844112944 0.0 58-59 5.012606705865251E-5 0.0 0.0 1.5627553296540801 0.0 60-61 5.012606705865251E-5 0.0 0.0 1.767019052918089 0.0 62-63 5.012606705865251E-5 0.0 0.0 1.9612074367033088 0.0 64-65 5.012606705865251E-5 0.0 0.0 2.153290525672065 0.0 66-67 5.012606705865251E-5 0.0 0.0 2.362842549010762 0.0 68-69 5.012606705865251E-5 0.0 0.0 2.5950515546599697 0.0 70-71 5.012606705865251E-5 0.0 0.0 2.8492408407143968 0.0 72-73 5.012606705865251E-5 0.0 0.0 3.1061118713564615 0.0 74-75 5.012606705865251E-5 0.0 0.0 3.362155821892059 0.0 76-77 5.012606705865251E-5 0.0 0.0 3.6301799024546737 0.0 78-79 5.012606705865251E-5 0.0 0.0 3.9215877933001497 0.0 80-81 5.012606705865251E-5 0.0 0.0 4.227281613257342 0.0 82-83 5.012606705865251E-5 0.0 0.0 4.536960455545698 0.0 84-85 5.012606705865251E-5 0.0 0.0 4.8842839741951005 0.0 86-87 5.012606705865251E-5 0.0 0.0 5.251482478433259 0.0 88-89 5.012606705865251E-5 0.0 0.0 5.619934134347885 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATAGC 1350 0.0 28.149715 1 TTCGGCG 35 0.008394812 27.142328 18-19 GATCGTA 70 0.008396903 27.140287 6 TAGCCCT 2475 0.0 25.906637 4 ACCGTGC 185 8.985808E-9 25.673244 8 GTGTAGC 1760 0.0 25.640633 1 GTAGCCC 1500 0.0 22.48233 3 ATAGCCC 1520 0.0 21.874025 3 TGTAGCC 1900 0.0 21.251183 2 GTACATG 9310 0.0 21.225672 1 TATAGCC 1645 0.0 21.0802 2 GAGTACT 7245 0.0 21.076334 12-13 TACATGG 9335 0.0 21.016169 2 AGTACTT 7445 0.0 19.967888 12-13 ACATGGG 9470 0.0 19.96215 3 TAGGACG 780 0.0 19.485334 4 GTACTTT 7955 0.0 19.314638 14-15 CATGGGG 3455 0.0 18.695768 4 AGGACGT 860 0.0 18.225018 5 CCCTGGC 3210 0.0 17.755327 7 >>END_MODULE