FastQCFastQC Report
Thu 26 May 2016
SRR938112_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938112_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2047029
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108700.5310134834435662No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA85750.41889978109738557No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84900.4147474217512307No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA66760.3261311881756438No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57350.28016212764938847No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT52980.25881411548151No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG31350.15314878294347564No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29120.14225494607062236No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT29060.14196183835207024No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA28700.1402031920407576No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC22560.11020850217559204No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG21870.10683776341224281No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGC550.002564987934.586843
GTATAGC13900.026.03231
TAGCCCT23800.023.9782734
TAGGACG6250.023.5882284
GTAGCCC14400.022.457433
GAGTACT73500.022.3440412-13
TACACCG1503.0067778E-522.1932245
ATAGCCC14000.022.0799943
GTACATG88300.021.5681551
GACGACC3550.021.4107794-95
TATAGCC16000.021.1033782
GTACTTT81450.020.22074714-15
AGTACTT75650.020.20100812-13
TACATGG92200.020.1162622
GTGTAGC19150.019.6413821
ATACCGA1951.0243957E-519.5067046
ACATGGG93100.019.4109843
AGGACGT7950.019.1424035
TGACGCG757.720052E-419.00205894-95
TATACCG1300.0075130318.2911195
Produced by FastQC (version 0.11.5)