##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938112_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2047029 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.09673385184089 33.0 31.0 34.0 30.0 34.0 2 32.217459547470995 34.0 31.0 34.0 30.0 34.0 3 32.24663255869849 34.0 31.0 34.0 30.0 34.0 4 35.7463186891832 37.0 35.0 37.0 35.0 37.0 5 35.76357247503577 37.0 35.0 37.0 35.0 37.0 6 35.79846304082648 37.0 36.0 37.0 35.0 37.0 7 35.74890927290234 37.0 36.0 37.0 35.0 37.0 8 35.74485852423195 37.0 36.0 37.0 35.0 37.0 9 37.40881589855346 39.0 37.0 39.0 35.0 39.0 10-11 37.32530926528154 39.0 37.0 39.0 34.5 39.0 12-13 37.19920553152886 39.0 37.0 39.0 34.0 39.0 14-15 38.40274172959934 40.0 38.0 41.0 34.0 41.0 16-17 38.24478231622513 40.0 38.0 41.0 33.5 41.0 18-19 38.45515500757439 40.0 38.0 41.0 34.0 41.0 20-21 38.46729137691747 40.0 38.0 41.0 34.0 41.0 22-23 38.362536143845546 40.0 38.0 41.0 34.0 41.0 24-25 38.27257308030321 40.0 38.0 41.0 34.0 41.0 26-27 38.10156182447831 40.0 38.0 41.0 33.5 41.0 28-29 37.937164300066094 40.0 38.0 41.0 33.0 41.0 30-31 37.759232771006175 40.0 37.5 41.0 33.0 41.0 32-33 37.51288525956399 40.0 37.0 41.0 32.5 41.0 34-35 37.31680596610991 40.0 37.0 41.0 32.0 41.0 36-37 37.089869269072395 40.0 36.0 41.0 31.0 41.0 38-39 36.83267261968443 39.0 36.0 41.0 30.5 41.0 40-41 36.73910579674249 39.0 36.0 41.0 30.0 41.0 42-43 36.58521471850179 39.0 36.0 41.0 30.0 41.0 44-45 36.27634586515384 39.0 35.5 41.0 30.0 41.0 46-47 35.912311452353634 39.0 35.0 41.0 28.5 41.0 48-49 35.55426278767912 39.0 35.0 40.0 27.0 41.0 50-51 34.872059702134166 38.5 34.5 39.5 25.5 40.5 52-53 34.95276862223251 38.5 35.0 40.0 26.0 40.5 54-55 35.09769597792703 39.0 35.0 40.0 26.0 41.0 56-57 34.861655599407726 38.5 34.5 40.0 24.5 41.0 58-59 34.52499695900742 38.0 34.0 40.0 23.5 41.0 60-61 34.092954960579455 38.0 34.0 40.0 22.5 41.0 62-63 33.59759021489192 38.0 33.0 40.0 20.0 41.0 64-65 33.108181173788935 37.0 32.5 40.0 18.0 41.0 66-67 32.60184345214455 36.5 31.5 39.0 13.5 41.0 68-69 31.976422659376098 36.0 31.0 39.0 8.0 40.0 70-71 31.4093757342959 35.5 30.5 38.5 6.5 40.0 72-73 30.705110430775527 35.0 29.5 38.0 2.0 40.0 74-75 30.032556939838173 34.5 29.0 37.0 2.0 39.0 76-77 29.260406178906113 34.0 28.5 36.5 2.0 39.0 78-79 28.49860798259331 34.0 26.5 36.0 2.0 38.5 80-81 27.7710029999575 33.5 26.0 35.5 2.0 37.5 82-83 27.018102821210643 33.0 25.5 35.0 2.0 37.0 84-85 26.252096819341592 32.0 23.0 35.0 2.0 36.0 86-87 25.47094765144998 31.5 20.5 35.0 2.0 36.0 88-89 24.569194671887892 31.0 15.5 34.0 2.0 35.0 90-91 23.646974224595745 31.0 4.5 34.0 2.0 35.0 92-93 22.71451674597673 30.0 2.0 34.0 2.0 35.0 94-95 21.619286292475582 29.0 2.0 34.0 2.0 35.0 96-97 20.2965837318377 28.5 2.0 34.0 2.0 35.0 98-99 18.703842007123495 25.5 2.0 33.5 2.0 35.0 100-101 14.604179764917838 9.5 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2074524709698906 1101 2 0.17432602659486207 1101 3 0.20388759647373433 1101 4 0.024042489631192154 1101 5 0.0866077732888968 1101 6 0.13097366903449625 1101 7 0.17182524058498672 1101 8 0.14790662509980024 1101 9 0.1533451471201701 1101 10-11 0.19269544563565688 1101 12-13 0.29825831653729296 1101 14-15 0.3472152690268757 1101 16-17 0.24577123671216583 1101 18-19 0.24448043126703567 1101 20-21 0.37150531253004715 1101 22-23 0.3157771656670363 1101 24-25 0.36582736822504813 1101 26-27 0.39983246434818653 1101 28-29 0.4470652809281006 1101 30-31 0.3595802483038 1101 32-33 0.34290227674897267 1101 34-35 0.27616180307548177 1101 36-37 0.2535571325489556 1101 38-39 0.39466708179530485 1101 40-41 0.4842207875338076 1101 42-43 0.4478899356145618 1101 44-45 0.6098949099496807 1101 46-47 0.6044539393469179 1101 48-49 0.569088402333854 1101 50-51 0.5357177521003464 1101 52-53 0.4690312053099035 1101 54-55 0.4710428613862945 1101 56-57 0.467081533909635 1101 58-59 0.39797555700042864 1101 60-61 0.431994246018661 1101 62-63 0.230083244332711 1101 64-65 0.3099926560566999 1101 66-67 0.31977748243613746 1101 68-69 0.2315968048852639 1101 70-71 -0.015750713366717406 1101 72-73 -0.1620134025886344 1101 74-75 0.06857146950664372 1101 76-77 -0.17981723827433171 1101 78-79 -0.21765317598310574 1101 80-81 -0.1296607997769783 1101 82-83 -0.33955106662338963 1101 84-85 -0.38011161097594837 1101 86-87 -0.32233045834957963 1101 88-89 -0.3274362410733609 1101 90-91 -0.32000920385253906 1101 92-93 -0.3533366208173234 1101 94-95 -0.5250764631087605 1101 96-97 -0.5869702192381574 1101 98-99 -0.9594537714930276 1101 100-101 -1.1479892178703608 1102 1 0.2444166553769449 1102 2 0.11836300007960432 1102 3 0.16334801424481782 1102 4 0.09432338302143961 1102 5 0.06511876628574953 1102 6 0.11237474136320458 1102 7 0.18971908655282732 1102 8 0.22800344159487906 1102 9 0.27749591021271414 1102 10-11 0.1880417901386977 1102 12-13 0.1415337087789439 1102 14-15 0.28103582369890034 1102 16-17 0.2686425061149933 1102 18-19 0.23791119724268128 1102 20-21 0.24792710135662333 1102 22-23 0.26159573471738184 1102 24-25 0.23380677728171406 1102 26-27 0.20795055303618426 1102 28-29 0.13251077863557015 1102 30-31 0.23218424930824 1102 32-33 0.21014928436594715 1102 34-35 0.21346649602489265 1102 36-37 0.17010754501884406 1102 38-39 0.07021237210528142 1102 40-41 0.11223322440778105 1102 42-43 0.09044054106641397 1102 44-45 0.1432162691007477 1102 46-47 0.12080247640410846 1102 48-49 -0.0798771096961346 1102 50-51 0.029766990402848137 1102 52-53 -0.05096655098409997 1102 54-55 -0.046370273477862156 1102 56-57 0.10160565898055296 1102 58-59 -0.08820501599326036 1102 60-61 -0.23580299271165472 1102 62-63 -0.2776595758080944 1102 64-65 -0.1991632338459226 1102 66-67 -0.40148564266267783 1102 68-69 -0.6245235135582305 1102 70-71 -0.9255133339223214 1102 72-73 -0.8278866343623008 1102 74-75 -0.7087032182345823 1102 76-77 -0.9768917595395514 1102 78-79 -0.8884625609519041 1102 80-81 -0.8819762927412746 1102 82-83 -0.6582044012587716 1102 84-85 -0.6729666861691648 1102 86-87 -0.9754694960438393 1102 88-89 -0.777175773545487 1102 90-91 -0.7760686050969241 1102 92-93 -0.8636451710809858 1102 94-95 -1.2270794647921193 1102 96-97 -1.4615685609882938 1102 98-99 -1.4675142562265293 1102 100-101 -1.5838264922209646 1103 1 0.255000774769357 1103 2 0.3394864002833202 1103 3 0.36554731897003023 1103 4 0.19112655825725966 1103 5 0.2331953572250285 1103 6 0.17444849806131657 1103 7 0.20019692190113147 1103 8 0.26498364619166637 1103 9 0.25385933521603476 1103 10-11 0.3264889359692589 1103 12-13 0.2342421805029673 1103 14-15 0.36073723196248864 1103 16-17 0.3732320947071699 1103 18-19 0.38088122735649677 1103 20-21 0.4189735563409016 1103 22-23 0.4021785255592434 1103 24-25 0.39333248473488425 1103 26-27 0.28304382835614916 1103 28-29 0.41780842931534323 1103 30-31 0.4790156562432699 1103 32-33 0.41947570923641564 1103 34-35 0.3925800206695129 1103 36-37 0.2248460419853231 1103 38-39 0.1918038095969834 1103 40-41 0.4464093569971297 1103 42-43 0.5291553344768047 1103 44-45 0.29620606140401406 1103 46-47 0.32285702447549625 1103 48-49 0.4086123836832556 1103 50-51 0.4112760577603609 1103 52-53 0.3783714433474188 1103 54-55 0.42255216131243856 1103 56-57 0.4030200117550109 1103 58-59 0.5187015094042025 1103 60-61 0.456457646764008 1103 62-63 0.3681585345501759 1103 64-65 0.2321830860690426 1103 66-67 0.26312066352394226 1103 68-69 0.046920399465996354 1103 70-71 -0.02800242873654568 1103 72-73 0.17373253662215404 1103 74-75 0.25567363620056227 1103 76-77 0.13950061679453896 1103 78-79 -0.03379879495393823 1103 80-81 0.005392789673141607 1103 82-83 -0.17177831295444435 1103 84-85 -0.2686314596512922 1103 86-87 -0.31514084585120017 1103 88-89 -0.36737743644792786 1103 90-91 -0.47694876983719325 1103 92-93 -0.5778166537434011 1103 94-95 -0.6322505710318786 1103 96-97 -0.7189547484910612 1103 98-99 -1.00860355696609 1103 100-101 -1.2758410730632654 1104 1 0.17198601664796342 1104 2 0.20801134283568246 1104 3 0.1991133922063284 1104 4 0.2209517040558424 1104 5 0.19533534988630663 1104 6 0.25745414783764176 1104 7 0.17244691734696005 1104 8 0.1882057622944373 1104 9 0.2090186922189261 1104 10-11 0.21431654362339714 1104 12-13 0.286581723992704 1104 14-15 0.245384205519656 1104 16-17 0.2656946789268346 1104 18-19 0.3990061834412586 1104 20-21 0.2286008168369662 1104 22-23 0.36440882095588734 1104 24-25 0.39036706781990915 1104 26-27 0.17197549755146468 1104 28-29 0.28895019940510025 1104 30-31 0.40004539368872116 1104 32-33 0.43051593429060375 1104 34-35 0.32556146827180044 1104 36-37 0.25519986215536505 1104 38-39 0.10312691382507211 1104 40-41 0.13872946067611736 1104 42-43 0.19856612864642642 1104 44-45 0.3469361603206451 1104 46-47 0.14800997961299345 1104 48-49 0.38996460859171833 1104 50-51 0.24509534298948665 1104 52-53 0.24629835798661048 1104 54-55 0.08358015516040496 1104 56-57 0.08547994154267968 1104 58-59 0.0869081803561258 1104 60-61 0.16314746597637964 1104 62-63 0.2118886100453352 1104 64-65 0.030168840617363912 1104 66-67 0.016201105825174977 1104 68-69 -0.08103883678626289 1104 70-71 -0.30107985227198597 1104 72-73 -0.19959055199163345 1104 74-75 -0.3361306749092776 1104 76-77 -0.36580707611370755 1104 78-79 -0.45102748988423613 1104 80-81 -0.5924964164174753 1104 82-83 -0.8511460897115057 1104 84-85 -0.7350895885890267 1104 86-87 -0.6147931673081182 1104 88-89 -0.8278660861487843 1104 90-91 -0.8648833354862404 1104 92-93 -0.9932608937561014 1104 94-95 -1.1620293943898403 1104 96-97 -1.3517198078384354 1104 98-99 -1.255109986156512 1104 100-101 -1.4313861034200794 1105 1 0.12955722783168966 1105 2 0.26507835250605893 1105 3 0.2092770672972577 1105 4 0.17322703222984615 1105 5 0.14323145719576047 1105 6 0.11340976376839507 1105 7 0.18828752615198852 1105 8 0.13580121039363746 1105 9 0.21642217638130035 1105 10-11 0.1845613618564812 1105 12-13 0.1805394943159513 1105 14-15 0.1127252979181037 1105 16-17 0.14295690303840303 1105 18-19 0.22459223245567728 1105 20-21 0.1590072941513867 1105 22-23 0.2990894875233252 1105 24-25 0.14346599814014382 1105 26-27 0.22281490421743655 1105 28-29 0.06411062150275626 1105 30-31 -0.08734384400577966 1105 32-33 -0.025975591749016758 1105 34-35 -0.026668761020665954 1105 36-37 0.09160043380608585 1105 38-39 -0.020103788856317806 1105 40-41 -0.010035745885225822 1105 42-43 0.06012762017495277 1105 44-45 0.2136809121000809 1105 46-47 0.15069662352446755 1105 48-49 0.14952012078509114 1105 50-51 0.22702293688450936 1105 52-53 0.31367290121109903 1105 54-55 0.22986741213791362 1105 56-57 0.18525399913285412 1105 58-59 0.11599272781672454 1105 60-61 0.13856222884137992 1105 62-63 0.22069682978938943 1105 64-65 0.2247864434176705 1105 66-67 0.08474829463667533 1105 68-69 0.10977697012283016 1105 70-71 -0.09030928362288293 1105 72-73 -0.11226473148816751 1105 74-75 -0.09672729428517002 1105 76-77 -0.1673154993005035 1105 78-79 -0.4254729503532708 1105 80-81 -0.2732833222110891 1105 82-83 -0.29666392791205 1105 84-85 -0.2856885674428291 1105 86-87 -0.29100547352852857 1105 88-89 -0.1516249086797039 1105 90-91 -0.22860793065408558 1105 92-93 -0.607367535188633 1105 94-95 -0.8466081404716874 1105 96-97 -0.9119229568952143 1105 98-99 -1.0795502697119979 1105 100-101 -1.0108955311272183 1106 1 0.2629512608680784 1106 2 0.19867429506481216 1106 3 0.2085814643695727 1106 4 0.13250711957473982 1106 5 0.1445384408141166 1106 6 0.11781646147883862 1106 7 0.2249471435200192 1106 8 0.12204780303054719 1106 9 0.22144417209575806 1106 10-11 0.2699894318689431 1106 12-13 0.3780242713564661 1106 14-15 0.38165893247418126 1106 16-17 0.03987881811217875 1106 18-19 0.23763716571865245 1106 20-21 0.35248437982694725 1106 22-23 0.3816447678098953 1106 24-25 0.40627561279402613 1106 26-27 0.3568540348201026 1106 28-29 0.2498799734504118 1106 30-31 0.3148732993885872 1106 32-33 0.3508889794534653 1106 34-35 0.23330625771248492 1106 36-37 0.25133308276612354 1106 38-39 0.24108000085334425 1106 40-41 0.12564417939481842 1106 42-43 0.04315404133426881 1106 44-45 0.3469361603206451 1106 46-47 0.49447122914894237 1106 48-49 0.5679815232168508 1106 50-51 0.4322761660098493 1106 52-53 0.4297247082397675 1106 54-55 0.2940661620672671 1106 56-57 0.1245938422948214 1106 58-59 0.26261189053016665 1106 60-61 0.476955565155599 1106 62-63 0.4359512397048917 1106 64-65 -0.038508218571344344 1106 66-67 0.024426006075053408 1106 68-69 0.03315516042479061 1106 70-71 -0.056014324477658306 1106 72-73 0.00677598562640469 1106 74-75 -0.022654816153568902 1106 76-77 -0.16283157455545094 1106 78-79 -0.11423708835686242 1106 80-81 -0.12262890703519247 1106 82-83 -0.05620706284838661 1106 84-85 -0.06050894463533396 1106 86-87 -0.012272819531947476 1106 88-89 -0.5030659763378615 1106 90-91 -0.3611990543578081 1106 92-93 -0.51249496259382 1106 94-95 -0.6066185549290353 1106 96-97 -0.5710499612305604 1106 98-99 -0.9442998498599486 1106 100-101 -0.8392743957666937 1107 1 0.04530150850060721 1107 2 0.04000406631022457 1107 3 0.013329603965210879 1107 4 0.03761174888443719 1107 5 0.03430474200157363 1107 6 0.006571444328564269 1107 7 0.11953968055252773 1107 8 0.076566570008751 1107 9 0.08418579992499531 1107 10-11 0.17560252956510425 1107 12-13 0.1380314067691799 1107 14-15 0.14414379781433695 1107 16-17 0.14014962668927922 1107 18-19 0.16779158841038821 1107 20-21 0.16419656986489883 1107 22-23 0.25449255597241205 1107 24-25 0.13565244405275934 1107 26-27 0.16794117394098151 1107 28-29 0.19564100454564226 1107 30-31 0.06686573697479758 1107 32-33 0.07909998540620222 1107 34-35 -0.010538044210989028 1107 36-37 0.13052689349789404 1107 38-39 0.09755707342386444 1107 40-41 0.1600655227064962 1107 42-43 0.13784012531301215 1107 44-45 -0.021223043659453822 1107 46-47 -0.08178779195108632 1107 48-49 -0.029423883403353557 1107 50-51 -0.09547328549041367 1107 52-53 -0.15119059104022625 1107 54-55 -0.08004296479981576 1107 56-57 -0.27940957371378516 1107 58-59 -0.26911681770729956 1107 60-61 -0.35166671025447727 1107 62-63 -0.27219773872312203 1107 64-65 -0.23684740912338498 1107 66-67 -0.3703055872288914 1107 68-69 -0.2851263849819965 1107 70-71 -0.188466125326606 1107 72-73 -0.1649795787313657 1107 74-75 -0.4816149561649752 1107 76-77 -0.5123333598132369 1107 78-79 -0.7590129268380892 1107 80-81 -0.6712550591655635 1107 82-83 -0.6656359208171523 1107 84-85 -0.706678155048543 1107 86-87 -0.5851972993350749 1107 88-89 -0.6391374540705996 1107 90-91 -0.6147815091083366 1107 92-93 -0.6393199005803396 1107 94-95 -0.8179012155952137 1107 96-97 -1.0455535022493514 1107 98-99 -1.3288072956912096 1107 100-101 -1.2993778873432547 1108 1 -0.07126423562154827 1108 2 -0.03445452671704885 1108 3 0.02192197456560052 1108 4 0.06553249000641159 1108 5 0.10175088039063951 1108 6 0.1012537414717869 1108 7 0.07422201052943933 1108 8 -0.03736488638688229 1108 9 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-0.749571034615439 2316 90-91 -0.7451473449117678 2316 92-93 -0.7388460865210824 2316 94-95 -0.7903021031234765 2316 96-97 -0.5453640111657982 2316 98-99 -0.5911492097564768 2316 100-101 -0.49999511976793265 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6151.0 3 1443.0 4 1221.0 5 1273.0 6 1584.0 7 1882.0 8 2428.0 9 2947.0 10 3958.0 11 5248.0 12 7292.0 13 9387.0 14 11387.0 15 14495.0 16 17906.0 17 24095.0 18 22931.0 19 18558.0 20 18176.0 21 19171.0 22 21244.0 23 23936.0 24 26816.0 25 30855.0 26 35358.0 27 41155.0 28 47826.0 29 56125.0 30 66076.0 31 79089.0 32 97559.0 33 122131.0 34 158200.0 35 211165.0 36 278268.0 37 342237.0 38 204962.0 39 12494.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.03143150116631 19.500221128105576 14.751299372231005 22.71704799849711 2 14.336294950912876 21.046416488347244 42.03105250655684 22.586236054183036 3 19.050074761819022 25.60200878353518 30.243504333054116 25.104412121591686 4 11.605470548038012 17.783879209266217 39.5938227164023 31.01682752629347 5 12.719763047256919 38.54099412659898 35.09688737647086 13.642355449673241 6 29.640954804123794 37.611785563595035 19.096180372339543 13.651079259941634 7 26.89479924466886 33.728989967249085 21.524050367805554 17.8521604202765 8 24.20188713741141 35.983541565027636 20.96993700742751 18.844634290133445 9 25.833211054047446 17.43947175348496 21.348593788212277 35.37872340425532 10-11 23.530947830252043 27.37436792448909 28.633518822836457 20.46116542242241 12-13 25.40194208198195 25.039471321583513 28.296986773743583 21.261599822690957 14-15 22.101153362504334 26.70868405919614 27.72531514878897 23.464847429510552 16-17 20.425514741491906 29.6978813538594 28.70337486486354 21.173229039785152 18-19 19.9182207560202 28.99379440429999 30.870735181608815 20.217249658070994 20-21 21.777379267365347 27.82356815689831 30.340102711004047 20.05894986473229 22-23 20.625286039789817 28.36885733375429 29.78798180113902 21.217874825316876 24-25 20.418666178395775 27.995081715201643 30.823095474257585 20.763156632144998 26-27 20.876077894356264 28.134073166799862 30.39396058706241 20.59588835178147 28-29 20.211506437370424 28.3269489116646 30.780518477159518 20.681026173805456 30-31 20.651457961391934 28.670551238549585 30.675452918881202 20.002537881177286 32-33 20.18684571899529 28.51901534262589 30.40564352331353 20.888495415065293 34-35 20.669177663892444 28.399662223117573 30.332540069442782 20.598620043547207 36-37 20.856236861411745 28.25481900009461 30.34462307668425 20.544321061809395 38-39 20.900550756633603 28.15242007161767 30.58491835424524 20.36211081750349 40-41 21.047864539201743 27.95004415042869 30.758099397975364 20.24399191239421 42-43 20.32916244657263 28.463613664892595 30.5726015853215 20.63462230321327 44-45 20.785095361702012 28.80098600576522 29.84150651281341 20.572412119719356 46-47 20.527201853168325 28.464261773955595 29.30805062244335 21.700485750432733 48-49 20.859293273837146 28.228825253282764 29.76414848829393 21.147732984586167 50-51 21.438046598424496 28.73764204288572 29.150360052963197 20.673951305726586 52-53 20.820749749875702 28.746617672029934 29.731404983917525 20.70122759417684 54-55 20.71953912428923 28.479954721805967 29.789082989494712 21.01142316441009 56-57 21.009398704279075 29.105003626914268 28.91436157879859 20.971236090008066 58-59 20.83097306335266 28.828585993412588 29.151896141929978 21.188544801304776 60-61 21.06681653247975 28.294706234499273 29.2622448106926 21.37623242232838 62-63 21.545734695024645 28.067898836012972 29.339731229701098 21.046635239261285 64-65 21.24569692680915 28.197738064286593 29.43492658629702 21.121638422607237 66-67 20.663314736203464 28.419546949384088 29.67514032146572 21.241997992946725 68-69 20.951075758442332 28.660347963726363 29.329369845522717 21.059206432308585 70-71 20.809275677398258 28.27679814736702 29.081557591987696 21.832368583247025 72-73 20.7359148458639 28.072702425825426 29.081703473731956 22.109679254578726 74-75 21.089561272946 27.996948393691422 29.008147309892234 21.90534302347034 76-77 21.13442599401103 27.720220102223475 29.307735362329957 21.837618541435543 78-79 20.784889722148726 27.72549137451471 29.507268343463643 21.98235055987292 80-81 20.697263566908518 28.427699877034833 29.01181496637331 21.863221589683338 82-83 20.879701044614148 28.256106612306404 28.962329100119078 21.901863242960374 84-85 21.121938134336087 27.764645064955623 28.912166744518302 22.201250056189988 86-87 21.005227083106835 27.82286308316058 29.162017588114647 22.009892245617934 88-89 21.616699537044617 27.276018050884765 29.2625881652463 21.844694246824314 90-91 21.064648006383056 27.677972562932545 29.29258942917568 21.964790001508714 92-93 20.821885794899387 27.802735088784168 29.36422862923127 22.01115048708517 94-95 20.810826534238934 28.109971532535187 28.813933853599107 22.26526807962677 96-97 20.88924704296798 27.616099237684193 28.63947807923499 22.855175640112833 98-99 21.25272988196743 27.274575780741355 28.70281483335256 22.769879503938657 100-101 22.15408538917809 26.50467095885506 28.729918888009493 22.61132476395736 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2486.0 1 1470.0 2 586.0 3 749.5 4 555.5 5 523.0 6 1275.5 7 2506.5 8 3440.0 9 3544.5 10 2802.5 11 2080.0 12 1984.0 13 2081.5 14 2099.5 15 2044.0 16 2090.0 17 2326.5 18 2726.0 19 3187.5 20 3748.5 21 4542.5 22 5566.0 23 6887.5 24 8704.5 25 10893.5 26 13545.0 27 16852.0 28 20368.0 29 24484.5 30 29324.0 31 34203.0 32 39273.5 33 45309.5 34 51688.0 35 57915.0 36 63196.5 37 66719.0 38 69700.5 39 72045.5 40 73146.5 41 74426.0 42 76154.0 43 77444.5 44 78868.5 45 80715.0 46 82645.5 47 85272.0 48 99579.0 49 99788.0 50 82248.0 51 72588.0 52 64474.0 53 57384.0 54 50771.5 55 43933.5 56 37215.0 57 30930.0 58 24597.0 59 19052.5 60 14416.0 61 10640.0 62 7716.5 63 5449.5 64 3790.0 65 2518.5 66 1729.5 67 1334.0 68 1016.5 69 756.5 70 541.0 71 391.0 72 289.0 73 221.0 74 187.0 75 157.5 76 108.5 77 69.5 78 53.0 79 43.0 80 27.0 81 18.5 82 10.5 83 6.0 84 7.0 85 6.0 86 3.5 87 2.5 88 2.5 89 2.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14484406425116597 2 0.05251513290725242 3 0.15656837299325022 4 0.13189847334844793 5 0.19980176147968592 6 0.19809196645479862 7 0.1671691021475514 8 0.06795213941766336 9 0.12354490336971288 10-11 0.10158625012151756 12-13 0.15339303937560242 14-15 0.14047187411609705 16-17 0.13673475070455768 18-19 0.13409678123758872 20-21 0.1343166120265028 22-23 0.19755460230411978 24-25 0.21724167073353626 26-27 0.14926510567266024 28-29 0.12698891906270013 30-31 0.09848419343350778 32-33 0.1251325701785368 34-35 0.09242663391676426 36-37 0.08624694618395734 38-39 0.050096994229197535 40-41 0.057766646197977646 42-43 0.061601472182367716 44-45 0.03876349577851609 46-47 0.04980388651064543 48-49 0.04140146524548505 50-51 0.05275938933937917 52-53 0.02669722803145437 54-55 0.049388650576029945 56-57 0.02557364844367129 58-59 0.04914439414390319 60-61 0.036833869964714716 62-63 0.05100074302806653 64-65 0.03991150100951183 66-67 0.036418634030099234 68-69 0.06624234439277607 70-71 0.05256398419367776 72-73 0.05104959431449189 74-75 0.04364862442105119 76-77 0.021225883951815044 78-79 0.02190980196176996 80-81 0.02513398686584313 82-83 0.02129916088145307 84-85 0.019589365856565785 86-87 0.02327763798167979 88-89 0.0668041341866676 90-91 0.10945130723599909 92-93 0.08048249438576591 94-95 0.07503557594933927 96-97 0.06616906746313804 98-99 0.056325533248429795 100-101 0.13165421691632118 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2047029.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.86911076634563 #Duplication Level Percentage of deduplicated Percentage of total 1 77.36906603444305 51.736006465458814 2 14.124323191210033 18.88961863945376 3 4.565748296767437 9.15922585763387 4 1.8824192166273286 5.035027964214016 5 0.8427702332211665 2.817764803792256 6 0.45031101493306247 1.8067138282118682 7 0.24753178133132434 1.158656107282462 8 0.1579988646539797 0.8452194865203054 9 0.09246070002347341 0.5564488312263149 >10 0.2489734087755634 2.442407921892888 >50 0.007882790769402195 0.36847040514746127 >100 0.008033591130878384 1.0729037813219853 >500 9.485702786277892E-4 0.45489105058777507 >1k 0.0010945041676474292 1.4295669732029435 >5k 3.6483472254914305E-4 1.6967050727382897 >10k+ 7.29669445098286E-5 0.5303728113149253 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10870 0.5310134834435662 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 8575 0.41889978109738557 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8490 0.4147474217512307 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6676 0.3261311881756438 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5735 0.28016212764938847 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 5298 0.25881411548151 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3135 0.15314878294347564 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2912 0.14225494607062236 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2906 0.14196183835207024 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2870 0.1402031920407576 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2256 0.11020850217559204 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2187 0.10683776341224281 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.4425643212675543E-5 0.0 12-13 0.0 0.0 0.0 4.8851286425351085E-5 0.0 14-15 0.0 0.0 0.0 4.8851286425351085E-5 0.0 16-17 0.0 0.0 0.0 4.8851286425351085E-5 0.0 18-19 0.0 0.0 0.0 7.327692963802663E-5 0.0 20-21 0.0 0.0 0.0 2.931077185521065E-4 0.0 22-23 0.0 0.0 0.0 7.327692963802662E-4 0.0 24-25 0.0 0.0 0.0 0.001538815522398559 0.0 26-27 0.0 0.0 0.0 0.00361499519547598 0.0 28-29 0.0 0.0 0.0 0.007107862174888582 0.0 30-31 0.0 0.0 0.0 0.018734468344122138 0.0 32-33 0.0 0.0 0.0 0.04179227553688785 0.0 34-35 0.0 4.8851286425351085E-5 0.0 0.08331586899843627 0.0 36-37 0.0 4.8851286425351085E-5 0.0 0.1488987210244701 0.0 38-39 0.0 4.8851286425351085E-5 0.0 0.23560975442946827 4.8851286425351085E-5 40-41 0.0 4.8851286425351085E-5 0.0 0.3281585165622959 4.8851286425351085E-5 42-43 0.0 4.8851286425351085E-5 0.0 0.4192905913887883 4.8851286425351085E-5 44-45 0.0 4.8851286425351085E-5 0.0 0.5289128781272762 4.8851286425351085E-5 46-47 0.0 4.8851286425351085E-5 0.0 0.6566834177727819 4.8851286425351085E-5 48-49 0.0 4.8851286425351085E-5 0.0 0.7953966455775663 4.8851286425351085E-5 50-51 0.0 4.8851286425351085E-5 0.0 0.9326199091463775 4.8851286425351085E-5 52-53 0.0 4.8851286425351085E-5 0.0 1.0647626389269522 4.8851286425351085E-5 54-55 0.0 4.8851286425351085E-5 0.0 1.2045994463195195 4.8851286425351085E-5 56-57 0.0 4.8851286425351085E-5 0.0 1.3649293683675219 4.8851286425351085E-5 58-59 0.0 4.8851286425351085E-5 0.0 1.551638985085214 4.8851286425351085E-5 60-61 0.0 4.8851286425351085E-5 0.0 1.7539565878158052 4.8851286425351085E-5 62-63 0.0 4.8851286425351085E-5 0.0 1.9471634256280688 4.8851286425351085E-5 64-65 0.0 4.8851286425351085E-5 0.0 2.1400527300785672 4.8851286425351085E-5 66-67 0.0 4.8851286425351085E-5 0.0 2.35360612868699 4.8851286425351085E-5 68-69 0.0 4.8851286425351085E-5 0.0 2.5851123750567284 4.8851286425351085E-5 70-71 0.0 4.8851286425351085E-5 0.0 2.83198235100724 4.8851286425351085E-5 72-73 0.0 4.8851286425351085E-5 0.0 3.084054988962052 4.8851286425351085E-5 74-75 0.0 4.8851286425351085E-5 0.0 3.3359810730575874 4.8851286425351085E-5 76-77 0.0 4.8851286425351085E-5 0.0 3.602415989221452 4.8851286425351085E-5 78-79 0.0 4.8851286425351085E-5 0.0 3.8977952925923374 4.8851286425351085E-5 80-81 0.0 4.8851286425351085E-5 0.0 4.200917524861641 4.8851286425351085E-5 82-83 0.0 4.8851286425351085E-5 0.0 4.50719066510538 4.8851286425351085E-5 84-85 0.0 4.8851286425351085E-5 0.0 4.846291869826954 4.8851286425351085E-5 86-87 0.0 4.8851286425351085E-5 0.0 5.203712306958035 4.8851286425351085E-5 88-89 0.0 7.327692963802663E-5 0.0 5.55021448157305 4.8851286425351085E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTACGC 55 0.0025649879 34.58684 3 GTATAGC 1390 0.0 26.0323 1 TAGCCCT 2380 0.0 23.978273 4 TAGGACG 625 0.0 23.588228 4 GTAGCCC 1440 0.0 22.45743 3 GAGTACT 7350 0.0 22.34404 12-13 TACACCG 150 3.0067778E-5 22.193224 5 ATAGCCC 1400 0.0 22.079994 3 GTACATG 8830 0.0 21.568155 1 GACGACC 355 0.0 21.41077 94-95 TATAGCC 1600 0.0 21.103378 2 GTACTTT 8145 0.0 20.220747 14-15 AGTACTT 7565 0.0 20.201008 12-13 TACATGG 9220 0.0 20.116262 2 GTGTAGC 1915 0.0 19.641382 1 ATACCGA 195 1.0243957E-5 19.506704 6 ACATGGG 9310 0.0 19.410984 3 AGGACGT 795 0.0 19.142403 5 TGACGCG 75 7.720052E-4 19.002058 94-95 TATACCG 130 0.00751303 18.291119 5 >>END_MODULE