FastQCFastQC Report
Thu 26 May 2016
SRR938112_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938112_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2047029
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99780.48743813595215313No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85650.418411268233132No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA83190.40639385177249565No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA68590.3350709735914831No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59300.28968812850233194No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT54240.2649693775711043No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG32730.15989026047017407No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT29240.14284116150772658No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA28380.13863995087514636No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27150.1326312426448282No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC25230.12325179565116078No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG20840.10180608091043167No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCCT28750.026.4288564
GTAGCCC17300.026.0792433
GTATAGC17300.025.5358181
TATACCG1401.7834545E-523.7446755
GTGTAGC20450.023.6929871
GAGTACT75450.022.37733812-13
GTACATG89400.022.3695641
ATAGCCC15950.022.0337543
TACATGG91900.021.3960742
TATAGCC18150.021.1961842
AGTACTT77650.020.73415212-13
TGTAGCC23950.020.62422
GTACTTT82000.020.53196114-15
ACATGGG93950.020.4221763
GGAACGC2801.5825208E-920.352588
CCCTGGC34900.020.1387527
AGCCCTG40050.019.3277515
AAGGCGG2650.018.81975294-95
GCCCTGG39500.018.6350636
TAGGACG7500.018.3625494
Produced by FastQC (version 0.11.5)