##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938112_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2047029 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.46521812832158 33.0 31.0 34.0 31.0 34.0 2 32.68122630407288 34.0 31.0 34.0 31.0 34.0 3 32.74553413752321 34.0 31.0 34.0 31.0 34.0 4 36.20171624339469 37.0 35.0 37.0 35.0 37.0 5 36.18387282251497 37.0 35.0 37.0 35.0 37.0 6 36.21201067498311 37.0 37.0 37.0 35.0 37.0 7 36.177242725921325 37.0 37.0 37.0 35.0 37.0 8 36.16628098576034 37.0 37.0 37.0 35.0 37.0 9 37.84758740594295 39.0 38.0 39.0 35.0 39.0 10-11 37.82401177511408 39.0 38.0 39.0 35.0 39.0 12-13 37.746500415968704 39.0 38.0 39.0 35.0 39.0 14-15 38.992817151100446 40.0 38.0 41.0 35.5 41.0 16-17 38.876569164384094 40.0 38.0 41.0 35.0 41.0 18-19 39.15574498456055 40.0 38.5 41.0 36.0 41.0 20-21 39.153670026169635 40.0 39.0 41.0 36.0 41.0 22-23 39.053188303634194 40.0 39.0 41.0 35.5 41.0 24-25 38.972329410086516 40.0 38.0 41.0 35.0 41.0 26-27 38.78683594614439 40.0 38.0 41.0 35.0 41.0 28-29 38.67146361873721 40.0 38.0 41.0 34.5 41.0 30-31 38.46448047389656 40.0 38.0 41.0 34.0 41.0 32-33 38.2758400100829 40.0 38.0 41.0 34.0 41.0 34-35 38.14504533155124 40.0 38.0 41.0 34.0 41.0 36-37 38.1561809822919 40.0 38.0 41.0 34.0 41.0 38-39 38.01183568967514 40.0 38.0 41.0 33.5 41.0 40-41 37.85370041166979 40.0 37.5 41.0 33.0 41.0 42-43 37.6671862000978 40.0 37.0 41.0 33.0 41.0 44-45 37.40674313847044 40.0 37.0 41.0 33.0 41.0 46-47 37.02511200378695 40.0 37.0 41.0 31.5 41.0 48-49 36.65867679451537 40.0 36.0 41.0 30.5 41.0 50-51 36.31813227853635 39.0 36.0 41.0 30.0 41.0 52-53 35.809392294881995 39.0 35.0 40.0 28.0 41.0 54-55 35.542286406299084 39.0 35.0 40.0 28.0 41.0 56-57 35.25736665186473 38.0 35.0 40.0 27.0 41.0 58-59 34.89141042945654 38.0 34.0 40.0 26.0 41.0 60-61 34.455978640263524 38.0 34.0 40.0 25.5 41.0 62-63 34.01049569888849 37.5 33.0 40.0 23.5 41.0 64-65 33.527568002211986 37.0 32.5 39.5 22.5 41.0 66-67 32.99680268330346 36.0 32.0 39.0 21.5 40.0 68-69 32.449749612731424 36.0 31.0 39.0 19.5 40.0 70-71 31.845327056920052 35.0 31.0 38.0 17.0 40.0 72-73 31.23826018097448 35.0 30.0 37.5 12.5 39.5 74-75 30.62148875272407 34.0 29.0 37.0 8.0 39.0 76-77 29.1854392390142 32.5 28.5 35.5 7.5 38.0 78-79 29.87049694948142 34.0 29.0 36.0 6.5 38.5 80-81 29.525405600018367 34.0 29.0 36.0 2.0 37.5 82-83 28.928939941739955 34.0 28.5 35.0 2.0 37.0 84-85 28.289820515488543 33.5 27.5 35.0 2.0 36.5 86-87 27.586981181018928 33.0 26.0 35.0 2.0 36.0 88-89 26.795335581469537 32.0 25.5 35.0 2.0 35.5 90-91 26.201467346090357 32.0 24.5 34.0 2.0 35.0 92-93 25.363995576027502 31.5 21.0 34.0 2.0 35.0 94-95 24.49922008921222 31.0 17.0 34.0 2.0 35.0 96-97 23.38209229082734 31.0 2.0 34.0 2.0 35.0 98-99 21.88040081503486 30.0 2.0 34.0 2.0 35.0 100-101 17.54229007014556 23.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.21897314137399349 1101 2 0.21013029022265783 1101 3 0.1728955855004628 1101 4 0.13624924302844477 1101 5 0.11856169606562617 1101 6 0.15657179934914467 1101 7 0.1265783414533317 1101 8 0.11355178728440052 1101 9 0.11749773933525631 1101 10-11 0.22103239137712194 1101 12-13 0.27782244081252827 1101 14-15 0.18326545305469466 1101 16-17 0.2987798022061696 1101 18-19 0.3628864602454982 1101 20-21 0.32195168847123057 1101 22-23 0.3615179341256791 1101 24-25 0.359205381812572 1101 26-27 0.42172870220561975 1101 28-29 0.4614028361903877 1101 30-31 0.4450901540136769 1101 32-33 0.42060543408009465 1101 34-35 0.4703370964014013 1101 36-37 0.6353867623105032 1101 38-39 0.5416709814791432 1101 40-41 0.34553607056364655 1101 42-43 0.37397886931228896 1101 44-45 0.40744544953096806 1101 46-47 0.5245693434147469 1101 48-49 0.5486569058755251 1101 50-51 0.422494651400271 1101 52-53 0.17507623825159868 1101 54-55 0.2723744161163566 1101 56-57 0.4832642958460198 1101 58-59 0.5443028720683927 1101 60-61 0.5364816296908188 1101 62-63 0.5355493513360372 1101 64-65 0.33180787707311055 1101 66-67 0.042606351415692245 1101 68-69 0.19043591693912987 1101 70-71 0.274162218902692 1101 72-73 0.25886265218379734 1101 74-75 0.2951970260241197 1101 76-77 0.14302712608703416 1101 78-79 0.15373120890998493 1101 80-81 0.2352683025070803 1101 82-83 0.2884448213351227 1101 84-85 0.006131914073261413 1101 86-87 -0.11610308751533438 1101 88-89 -0.03195955020376573 1101 90-91 -0.1741704172463585 1101 92-93 -0.05665544839234826 1101 94-95 -0.22384583388673107 1101 96-97 -0.256747971263394 1101 98-99 -0.3013333630037849 1101 100-101 -0.7248945185952564 1102 1 0.15335113491956065 1102 2 0.1242329705794134 1102 3 0.1255394384435604 1102 4 0.10274071462057321 1102 5 0.09577587349743055 1102 6 0.11974014472641414 1102 7 0.09256468856045785 1102 8 0.12106587729304863 1102 9 0.09602093902276465 1102 10-11 0.17211088156062004 1102 12-13 0.17630782300982162 1102 14-15 0.16926670394985877 1102 16-17 0.13735009646879348 1102 18-19 0.17232054644100714 1102 20-21 0.1707910065706102 1102 22-23 0.19109073482878358 1102 24-25 0.16915011456742235 1102 26-27 0.1243882494652766 1102 28-29 0.11937688592858109 1102 30-31 0.15601363085118436 1102 32-33 0.1146238068933485 1102 34-35 0.2948045940530619 1102 36-37 0.320978740305911 1102 38-39 0.3972684466726406 1102 40-41 0.3966937298551443 1102 42-43 0.5110042034268432 1102 44-45 0.32898578276199686 1102 46-47 0.40183630025187966 1102 48-49 0.4518351793611899 1102 50-51 0.4352512244125606 1102 52-53 0.3494790404427519 1102 54-55 0.4134314457751245 1102 56-57 0.3628150663788361 1102 58-59 0.45548873462804096 1102 60-61 0.3447778779353783 1102 62-63 0.4076476465845005 1102 64-65 0.3304950765560122 1102 66-67 0.17409711385721494 1102 68-69 0.23510804522796036 1102 70-71 0.20512146715654822 1102 72-73 -0.025982508851516428 1102 74-75 -0.0013773115594695184 1102 76-77 -0.09493155484634741 1102 78-79 -0.09481328854637283 1102 80-81 -0.1513193345566144 1102 82-83 0.11550711265883606 1102 84-85 -0.19261335438092786 1102 86-87 -0.44797655877264475 1102 88-89 -0.5474152550297084 1102 90-91 -0.5494607621028074 1102 92-93 -0.45614672002725243 1102 94-95 -0.6621651353385154 1102 96-97 -0.35571720342767676 1102 98-99 -0.5565337038610885 1102 100-101 -1.0640917136139976 1103 1 0.12543939496119094 1103 2 0.11254449354120055 1103 3 0.10261419155457929 1103 4 0.04934017877184971 1103 5 0.08911540673333462 1103 6 0.01095621133107727 1103 7 0.03441181667701443 1103 8 0.02294079425863771 1103 9 0.09529687897227745 1103 10-11 0.11992818371730607 1103 12-13 0.1479496347099314 1103 14-15 0.10702466418190681 1103 16-17 0.11477996007093338 1103 18-19 0.22462032280166966 1103 20-21 0.17683591157924639 1103 22-23 0.18543360051232582 1103 24-25 0.24035322780421353 1103 26-27 0.30897097387961026 1103 28-29 0.26351991546668785 1103 30-31 0.2537473450356771 1103 32-33 0.22773161877059067 1103 34-35 0.08150884762908106 1103 36-37 0.08610899361621449 1103 38-39 0.21250941252888822 1103 40-41 0.2954991414843988 1103 42-43 0.17896288550469563 1103 44-45 0.17750583280407284 1103 46-47 0.29920244878383784 1103 48-49 0.32828163877398 1103 50-51 0.17810251532084465 1103 52-53 0.2062153453008264 1103 54-55 0.014105685179934824 1103 56-57 0.032589987772929874 1103 58-59 0.11684285977749909 1103 60-61 0.15831968299272603 1103 62-63 0.12133503863977069 1103 64-65 0.08055679880038014 1103 66-67 -0.03970292821355059 1103 68-69 0.17979301267847347 1103 70-71 -0.14225769009954803 1103 72-73 -0.3330174608022709 1103 74-75 -0.30355668802641134 1103 76-77 -0.10060584960742958 1103 78-79 -0.33236034137840065 1103 80-81 -0.3354215665215392 1103 82-83 -0.5337591399429051 1103 84-85 -0.40395197977395725 1103 86-87 -0.647672376052661 1103 88-89 -0.7631671592854978 1103 90-91 -0.7948349151518315 1103 92-93 -0.8309618300755233 1103 94-95 -0.8071147057738379 1103 96-97 -1.1284163199415609 1103 98-99 -1.315408698325463 1103 100-101 -1.80456379190991 1104 1 0.13846094530561004 1104 2 0.19827744206863684 1104 3 0.15259011949976298 1104 4 0.09115841263771784 1104 5 0.0889545793833193 1104 6 0.07908819385346533 1104 7 0.034649154125588666 1104 8 0.08406845942545971 1104 9 0.16650800260374865 1104 10-11 0.16735845933582283 1104 12-13 0.16536179773369497 1104 14-15 0.19849626045841262 1104 16-17 0.24873769214506325 1104 18-19 0.21166734528366504 1104 20-21 0.2013451669590296 1104 22-23 0.18726945251992788 1104 24-25 0.18792182014852443 1104 26-27 0.024041121148535183 1104 28-29 0.05433271815651608 1104 30-31 0.08286984707204681 1104 32-33 -0.06776183232930322 1104 34-35 0.012182500830142828 1104 36-37 -0.05375094606051789 1104 38-39 0.026378689560509372 1104 40-41 0.0933774175609301 1104 42-43 -0.03558847645312824 1104 44-45 0.06129063644116428 1104 46-47 0.09744570925908391 1104 48-49 -0.004646069592432411 1104 50-51 0.16682482269886378 1104 52-53 0.007406326477777725 1104 54-55 -0.006372737150606156 1104 56-57 0.15909839477542675 1104 58-59 0.08816510521877063 1104 60-61 0.04481291809472765 1104 62-63 0.04097679441640878 1104 64-65 0.1135461836747993 1104 66-67 -0.15833378780711627 1104 68-69 -0.039439518166282994 1104 70-71 -0.13121160968066903 1104 72-73 -0.4099146640198619 1104 74-75 -0.35540177849830457 1104 76-77 -0.1618769061494305 1104 78-79 -0.2321245653604116 1104 80-81 -0.18392519554645403 1104 82-83 -0.2689772067357019 1104 84-85 -0.43810723281679387 1104 86-87 -0.5260489046281407 1104 88-89 -0.7443899155748106 1104 90-91 -0.5950372505315045 1104 92-93 -1.008973114013866 1104 94-95 -0.9548361881960297 1104 96-97 -0.9126830851518442 1104 98-99 -0.6835823189967236 1104 100-101 -1.2385294612561637 1105 1 0.052355183948385786 1105 2 0.05251994761515277 1105 3 -0.01271520142158522 1105 4 0.07426250991590422 1105 5 0.059389674078524024 1105 6 -0.04390033878523525 1105 7 0.014830535256436406 1105 8 -0.046304392839857655 1105 9 0.0502088885030858 1105 10-11 0.04684140513901269 1105 12-13 0.0996121333619584 1105 14-15 0.051203736469148 1105 16-17 0.016841458399447617 1105 18-19 0.07569048409613544 1105 20-21 0.06887491803411194 1105 22-23 0.07089604473195976 1105 24-25 -0.018586008190993653 1105 26-27 0.1143212668388287 1105 28-29 0.020983314820689714 1105 30-31 0.009303043233764186 1105 32-33 0.11330395479301814 1105 34-35 0.12403569152623817 1105 36-37 0.2092378802684678 1105 38-39 0.24891055869012746 1105 40-41 0.2165511244152185 1105 42-43 0.1613783997623912 1105 44-45 0.25173261045803486 1105 46-47 0.2717983088412481 1105 48-49 0.39643792134996403 1105 50-51 0.3656161876070527 1105 52-53 0.35880165401461994 1105 54-55 0.4217444492566145 1105 56-57 0.37347923840327013 1105 58-59 0.47290411161972656 1105 60-61 0.34044454883743214 1105 62-63 0.4317226383254962 1105 64-65 0.24372763947266662 1105 66-67 0.1779198142544871 1105 68-69 -0.03674416310219186 1105 70-71 -0.10902106839951031 1105 72-73 -0.04689181441766266 1105 74-75 -0.12472498167374013 1105 76-77 -0.06854748370880515 1105 78-79 -0.03335784056961799 1105 80-81 -0.14511078839514369 1105 82-83 -0.20960348385289862 1105 84-85 -0.08799456027997365 1105 86-87 -0.096384741961554 1105 88-89 -0.30759579893795674 1105 90-91 -0.47643291655885633 1105 92-93 -0.45128873251317003 1105 94-95 -0.49444094577959063 1105 96-97 -0.76187349551682 1105 98-99 -1.0945518503715554 1105 100-101 -1.8287376777148694 1106 1 0.24155218507713272 1106 2 0.20374615063607138 1106 3 0.21827701487678297 1106 4 0.13857052490690336 1106 5 0.1292882548358989 1106 6 0.15742830197978464 1106 7 0.19830797908635844 1106 8 0.19970372495263433 1106 9 0.15275044140444294 1106 10-11 0.14316730098660457 1106 12-13 0.121710397488485 1106 14-15 0.24408650974791612 1106 16-17 0.21306318169274618 1106 18-19 0.2352423770258838 1106 20-21 0.252477436083133 1106 22-23 0.3367589253339247 1106 24-25 0.32121574217652693 1106 26-27 0.33619425746693565 1106 28-29 0.29788210655336655 1106 30-31 0.26913661897417995 1106 32-33 0.39456397542047483 1106 34-35 0.3340064851571256 1106 36-37 0.295935999230565 1106 38-39 0.3367442788115156 1106 40-41 0.35527801996117603 1106 42-43 0.4671006432189344 1106 44-45 0.3627918016223646 1106 46-47 0.3077039832934858 1106 48-49 0.2791808409459904 1106 50-51 0.3688363590846464 1106 52-53 0.31625109688872755 1106 54-55 0.16596333417657405 1106 56-57 0.2185538324436962 1106 58-59 0.3630729981905674 1106 60-61 0.2119267314972717 1106 62-63 0.22768811336409556 1106 64-65 0.19489049394055513 1106 66-67 0.26571729922739706 1106 68-69 0.060898649551177186 1106 70-71 -0.35043725552173655 1106 72-73 -0.15789392473032748 1106 74-75 -0.0357303377603202 1106 76-77 -0.22058051337574014 1106 78-79 -0.017650113557110103 1106 80-81 -0.05379457670576926 1106 82-83 0.15106047837524628 1106 84-85 -0.014910923961107159 1106 86-87 -0.3620110448180043 1106 88-89 -0.3232868773687265 1106 90-91 -0.6041465654299145 1106 92-93 -0.7995509003879562 1106 94-95 -1.0977993984184273 1106 96-97 -1.1681868895386671 1106 98-99 -1.1693084592988896 1106 100-101 -1.795144477275457 1107 1 0.037794013765420686 1107 2 0.07811232091398779 1107 3 0.05565140773462929 1107 4 0.03101962890694665 1107 5 -0.05280523030755546 1107 6 0.04030921081786687 1107 7 0.00894142956406796 1107 8 0.008179696309852602 1107 9 0.03686740598863736 1107 10-11 0.06348805745271591 1107 12-13 -0.01518699022005876 1107 14-15 0.028781049471660936 1107 16-17 -0.026622073566805682 1107 18-19 0.0277001896005018 1107 20-21 0.03736721643041108 1107 22-23 0.10755626556669995 1107 24-25 0.11824134257656027 1107 26-27 0.06550507211195367 1107 28-29 0.018002565804266624 1107 30-31 0.0291030207316183 1107 32-33 0.00854797973543242 1107 34-35 0.1433673600598624 1107 36-37 -0.008203944753645942 1107 38-39 -0.024574480300884716 1107 40-41 0.010235867651019248 1107 42-43 0.10201331868969277 1107 44-45 0.016533258089332037 1107 46-47 -0.017374464108137033 1107 48-49 -0.005169569621166659 1107 50-51 -0.04767650291815784 1107 52-53 -0.07376241544795192 1107 54-55 -0.03405576850312997 1107 56-57 0.024636350401614493 1107 58-59 -0.029818557582238725 1107 60-61 -0.13359439241450133 1107 62-63 0.03163000750716094 1107 64-65 -0.22252834073979244 1107 66-67 -0.19112188149238563 1107 68-69 -0.2767524512407462 1107 70-71 -0.4657893488102367 1107 72-73 -0.5168023751378037 1107 74-75 -0.39933580000303337 1107 76-77 -0.45135493039285635 1107 78-79 -0.5210590991205386 1107 80-81 -0.22868382330170078 1107 82-83 -0.18774099702583769 1107 84-85 -0.40123733819147844 1107 86-87 -0.647492165471089 1107 88-89 -0.8308998570777426 1107 90-91 -1.1282201556942901 1107 92-93 -0.9275107622868148 1107 94-95 -1.249640419175737 1107 96-97 -1.5214586448221006 1107 98-99 -1.56611465980399 1107 100-101 -2.068803005638724 1108 1 -0.06808580180776147 1108 2 -0.11316852941283173 1108 3 -0.0955756281796738 1108 4 -0.057148259583286176 1108 5 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62-63 -0.06214092646033009 2316 64-65 -0.04172870947505203 2316 66-67 -0.06149261432107522 2316 68-69 0.018807105854790507 2316 70-71 0.12182768232213803 2316 72-73 0.35399300960777325 2316 74-75 0.4641289182923245 2316 76-77 0.5054888830926529 2316 78-79 0.45974233590742486 2316 80-81 0.664113826893729 2316 82-83 0.788373148181325 2316 84-85 0.8344774807020592 2316 86-87 1.013789788242427 2316 88-89 0.8552878452099826 2316 90-91 1.0208545425543534 2316 92-93 1.083784345465972 2316 94-95 1.6093917428820248 2316 96-97 1.8867190611633404 2316 98-99 2.4999346401152103 2316 100-101 2.8735526554049997 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 6.0 4 16.0 5 49.0 6 58.0 7 185.0 8 388.0 9 771.0 10 1334.0 11 2432.0 12 3775.0 13 5944.0 14 7607.0 15 10497.0 16 14558.0 17 20578.0 18 21325.0 19 16137.0 20 14624.0 21 14818.0 22 16735.0 23 18461.0 24 20979.0 25 24320.0 26 27961.0 27 33246.0 28 39842.0 29 48026.0 30 58406.0 31 71101.0 32 89026.0 33 113426.0 34 152023.0 35 215476.0 36 304677.0 37 415657.0 38 251208.0 39 11356.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.26894028407678 19.898321364483124 15.09789964144748 22.73483870999262 2 14.548548579848871 21.389575884546947 41.42989338239965 22.63198215320454 3 19.050240954908407 25.53824011068683 30.513871029520395 24.89764790488437 4 11.800137467654311 18.139819357536563 39.2491647643143 30.81087841049483 5 12.67476141973273 38.636642314014864 35.19717831661379 13.491417949638617 6 29.65452621561652 37.75267637394686 18.894022724141738 13.698774686294874 7 26.83938527495214 33.7945871797615 21.395007105419612 17.971020439866752 8 24.239470960108527 36.22103057650868 20.787785615152497 18.75171284823029 9 25.53007309617988 17.349778630395562 21.372584364950377 35.747563908474184 10-11 23.52201654202261 27.414731300826713 28.55423640798445 20.50901574916623 12-13 25.3017262962488 25.028205738851128 28.2772332850923 21.392834679807766 14-15 21.924335219481502 26.767842565982214 27.589057116435576 23.71876509810071 16-17 20.3780556079364 29.788482902078872 28.66714477076895 21.16631671921578 18-19 19.9206508554593 28.973526999373238 30.828898857808074 20.276923287359388 20-21 21.7087056412 27.860401586885185 30.282423942210883 20.148468829703926 22-23 20.55618166620991 28.434037817734875 29.67996545237024 21.32981506368498 24-25 20.398673352340342 28.08945345399333 30.73314919070389 20.778724002962438 26-27 20.831655787046085 28.16477250517922 30.399630614079665 20.603941093695035 28-29 20.163075472481626 28.349312742897204 30.765790302920752 20.72182148170042 30-31 20.626795950689946 28.655849681919488 30.6582034093385 20.059150958052065 32-33 20.24665991541888 28.516767471296205 30.395270413853442 20.841302199431468 34-35 20.659319076879033 28.422264770213975 30.361401165406754 20.55701498750024 36-37 20.765873151572293 28.29214317467917 30.34777553602572 20.594208137722823 38-39 20.861303577439205 28.150917919048034 30.61324388717551 20.374534616337243 40-41 21.092419342743785 27.920626464505382 30.716990570740936 20.269963622009893 42-43 20.32335716946451 28.467326587565722 30.539250230524495 20.67006601244527 44-45 20.74882671422828 28.762294036870017 29.913059365548804 20.575819883352896 46-47 20.51823988471074 28.441103552716747 29.317546195087996 21.72311036748452 48-49 20.84387204499817 28.217603522003852 29.758089394980452 21.180435038017524 50-51 21.398484765582253 28.701835516750823 29.200505729516784 20.69917398815014 52-53 20.800772304330312 28.701355709704835 29.757338246872102 20.740533739092754 54-55 20.698984749006172 28.4633269219589 29.77358744979562 21.064100879239312 56-57 21.028078251875115 29.064950776210953 28.82754108741357 21.07942988450036 58-59 20.740505110621825 28.876137173213873 29.119134805294056 21.26422291087025 60-61 20.983096802559906 28.441022008353894 29.205110041769462 21.370771147316738 62-63 21.492405897034477 28.243324987735686 29.153254099017488 21.11101501621235 64-65 21.22337583459907 28.34378946396318 29.27635139146468 21.15648330997307 66-67 20.577155998579205 28.56137775968942 29.510226735843244 21.351239505888138 68-69 20.852114685812243 28.79775073349896 29.207992157767272 21.142142422921527 70-71 20.743186080639596 28.441259223193526 28.894384524749782 21.921170171417096 72-73 20.70399205833722 28.175559796602368 28.883914493265877 22.23653365179453 74-75 21.021221228361878 28.150411618442966 28.79386928176747 22.034497871427686 76-77 20.949188170218566 27.875546551919168 29.313994734143122 21.861270543719144 78-79 20.670970207253568 27.814349158508044 29.48443271032901 22.03024792390938 80-81 20.509299645808042 28.532007603193616 29.04303191636194 21.9156608346364 82-83 20.62913815857151 28.414111394091364 28.989962495274952 21.966787952062173 84-85 20.909992646178576 27.891871950668317 28.903791982135836 22.29434342101727 86-87 20.816134052986897 27.98033576774313 29.076797525356056 22.126732653913912 88-89 21.371337463374328 27.51279653875951 29.067388792697173 22.048477205168993 90-91 20.766133613432444 27.834116042246492 29.371069505089014 22.028680839232052 92-93 20.49275312038618 28.045300724187104 29.29499440875074 22.166951746675974 94-95 20.431980976303194 28.37531248312842 28.811646786392114 22.381059754176274 96-97 20.397986614563333 27.940242399724042 28.650995601704597 23.010775384008028 98-99 20.71695278576794 27.63518440482952 28.760386703553742 22.8874761058488 100-101 21.30774388895878 27.38120218926304 28.738901924093984 22.572151997684198 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2349.0 1 1372.5 2 546.5 3 715.0 4 542.0 5 510.5 6 1253.5 7 2546.0 8 3390.5 9 3468.5 10 2825.0 11 2070.5 12 1898.5 13 1911.0 14 1956.0 15 1987.5 16 2040.5 17 2262.5 18 2646.0 19 3153.5 20 3773.0 21 4463.5 22 5457.0 23 6786.0 24 8495.0 25 10744.0 26 13356.5 27 16421.5 28 19963.5 29 24341.5 30 29226.5 31 34075.0 32 39197.0 33 45281.5 34 51869.0 35 57717.0 36 62597.0 37 66705.5 38 70166.5 39 72174.5 40 73427.5 41 74734.5 42 76402.0 43 78306.5 44 79772.5 45 81660.0 46 83573.0 47 86113.0 48 102748.5 49 101535.0 50 81396.0 51 73340.5 52 65363.0 53 57542.5 54 50700.5 55 43856.5 56 36631.0 57 30090.5 58 24001.5 59 18360.0 60 13664.5 61 9987.0 62 7080.5 63 4849.0 64 3252.0 65 2138.5 66 1492.0 67 1116.0 68 786.5 69 585.5 70 433.5 71 296.0 72 188.0 73 138.5 74 129.5 75 94.5 76 58.5 77 38.0 78 22.0 79 13.0 80 9.0 81 5.5 82 5.0 83 3.5 84 1.0 85 1.5 86 1.5 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009184041847966003 2 0.013434103766971546 3 0.008451272551585738 4 9.770257285070217E-5 5 0.001172430874208426 6 0.0021006053162900967 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 8.060462260182929E-4 14-15 0.0 16-17 0.0026868207533943095 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0031753336176478203 26-27 0.01900315041946157 28-29 0.006057559516743534 30-31 0.003077631044797118 32-33 0.0 34-35 0.009281744420816706 36-37 9.281744420816705E-4 38-39 0.003028779758371767 40-41 0.0024181386780548785 42-43 0.0032486105472858467 44-45 0.0 46-47 1.9540514570140434E-4 48-49 6.106410803168885E-4 50-51 0.004152359346154842 52-53 0.008182590476246306 54-55 0.007132287818101258 56-57 0.017391057967424985 58-59 0.024767602217653 60-61 0.0038592516276027356 62-63 0.021543417313579828 64-65 0.022593719971724876 66-67 0.013971467917650408 68-69 0.03287691576426128 70-71 0.01731778103778696 72-73 0.005007256858598486 74-75 0.01719565282172358 76-77 0.02190980196176996 78-79 0.0280650640513642 80-81 0.012579206254527905 82-83 0.036809444321502044 84-85 0.023155509765616412 86-87 0.03292576705068663 88-89 0.023424191840955844 90-91 0.029823710362676836 92-93 0.025769053589372693 94-95 0.01555913472647432 96-97 0.015974370661089803 98-99 0.011797585671722286 100-101 0.010820559943215265 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2047029.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.42207980062909 #Duplication Level Percentage of deduplicated Percentage of total 1 76.0104451883004 49.727614107903314 2 14.837216373215629 19.413631071754267 3 4.919687593174115 9.655685829444053 4 1.9914189966131621 5.2113109005166 5 0.9406063199503947 3.076821086238539 6 0.4764948567924996 1.8703970727400938 7 0.2701620133223494 1.2372192556271353 8 0.16413295955539994 0.8590335662357441 9 0.10588091768905658 0.6234254861775569 >10 0.26371118425782575 2.545012661253251 >50 0.008287476831228996 0.37879724328746917 >100 0.00919297567463771 1.171213514164166 >500 0.0011201937662360013 0.5210592364243635 >1k 0.0011948733506517023 1.5065525189293334 >5k 4.4807750649438833E-4 2.202226449304051 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9978 0.48743813595215313 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8565 0.418411268233132 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 8319 0.40639385177249565 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6859 0.3350709735914831 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5930 0.28968812850233194 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 5424 0.2649693775711043 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3273 0.15989026047017407 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2924 0.14284116150772658 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2838 0.13863995087514636 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2715 0.1326312426448282 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2523 0.12325179565116078 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2084 0.10180608091043167 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 2.4425643212675543E-5 0.0 20-21 0.0 0.0 0.0 2.442564321267554E-4 0.0 22-23 0.0 0.0 0.0 6.839180099549152E-4 0.0 24-25 0.0 0.0 0.0 0.0014899642359732079 0.0 26-27 0.0 0.0 0.0 0.0035417182658379533 0.0 28-29 0.0 0.0 0.0 0.00701015960203788 0.0 30-31 0.0 0.0 0.0 0.018807745273760168 0.0 32-33 0.0 0.0 0.0 0.042012106325801934 0.0 34-35 0.0 0.0 0.0 0.08365782800341373 0.0 36-37 0.0 0.0 0.0 0.14992459803940247 0.0 38-39 0.0 0.0 0.0 0.2365623545147626 0.0 40-41 0.0 0.0 0.0 0.32952635258220575 0.0 42-43 0.0 0.0 0.0 0.42085383255439957 0.0 44-45 0.0 0.0 0.0 0.5308669295842902 0.0 46-47 0.0 0.0 0.0 0.6594923667422397 0.0 48-49 0.0 0.0 0.0 0.7988162356273409 0.0 50-51 0.0 0.0 0.0 0.9371386531407224 0.0 52-53 0.0 0.0 0.0 1.0697454701423381 0.0 54-55 0.0 0.0 0.0 1.2103883237609239 0.0 56-57 0.0 0.0 0.0 1.37230591261775 0.0 58-59 0.0 0.0 0.0 1.561335965440646 0.0 60-61 0.0 0.0 0.0 1.7654366401257628 0.0 62-63 0.0 0.0 0.0 1.9597670575258093 0.0 64-65 0.0 0.0 0.0 2.1539264954233674 0.0 66-67 0.0 0.0 0.0 2.369629350634505 0.0 68-69 0.0 0.0 0.0 2.6020881970895378 0.0 70-71 0.0 0.0 0.0 2.8501061782710453 0.0 72-73 0.0 0.0 0.0 3.103180267597577 0.0 74-75 0.0 0.0 0.0 3.3569382749340626 0.0 76-77 0.0 0.0 0.0 3.625864606705621 0.0 78-79 0.0 0.0 0.0 3.9241505616188146 0.0 80-81 0.0 0.0 0.0 4.230301573646489 0.0 82-83 0.0 0.0 0.0 4.540946904025297 0.0 84-85 0.0 0.0 0.0 4.883028037218818 0.0 86-87 0.0 0.0 0.0 5.24538245427886 0.0 88-89 0.0 0.0 0.0 5.598064316626682 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGCCCT 2875 0.0 26.428856 4 GTAGCCC 1730 0.0 26.079243 3 GTATAGC 1730 0.0 25.535818 1 TATACCG 140 1.7834545E-5 23.744675 5 GTGTAGC 2045 0.0 23.692987 1 GAGTACT 7545 0.0 22.377338 12-13 GTACATG 8940 0.0 22.369564 1 ATAGCCC 1595 0.0 22.033754 3 TACATGG 9190 0.0 21.396074 2 TATAGCC 1815 0.0 21.196184 2 AGTACTT 7765 0.0 20.734152 12-13 TGTAGCC 2395 0.0 20.6242 2 GTACTTT 8200 0.0 20.531961 14-15 ACATGGG 9395 0.0 20.422176 3 GGAACGC 280 1.5825208E-9 20.35258 8 CCCTGGC 3490 0.0 20.138752 7 AGCCCTG 4005 0.0 19.327751 5 AAGGCGG 265 0.0 18.819752 94-95 GCCCTGG 3950 0.0 18.635063 6 TAGGACG 750 0.0 18.362549 4 >>END_MODULE