FastQCFastQC Report
Thu 26 May 2016
SRR938111_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938111_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2792744
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145620.5214226581455371No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113490.40637451911095324No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74000.2649723712592346No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA36230.1297290406854334No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA35940.12869063544671477No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA35840.12833256467474283No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA32680.11701752828042956No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC30630.10967707745500482No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28420.10176371339442498No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68000.054.5546881
GTATCAA119350.042.0694961
TATCAAC149050.033.320482
ATCAACG149300.033.2010233
TCAACGC152550.032.5564
CAACGCA153000.032.4291845
AACGCAG157750.031.4766336
CGCAGAG175400.027.9510488
ACGCAGA176500.027.884517
TGGTATC27850.026.1091792
GCAGAGT190650.025.0415769
GTGGTAT30400.024.9393371
GAGTACT120400.024.58046512-13
GTACATG106000.024.33621
TACATGG102750.023.8668332
CAGAGTA181250.022.76292610-11
GTACTTT129050.022.71160914-15
TAAGGTG18200.022.1959885
ACATGGG105250.021.854973
AGAGTAC172650.021.2966210-11
Produced by FastQC (version 0.11.5)