FastQCFastQC Report
Thu 26 May 2016
SRR938111_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938111_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2792744
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139820.5006545533711647No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116410.4168301856525339No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75160.26912599221410916No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA35680.12775965143958773No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA35670.12772384436239054No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA35090.1256470338849533No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC33880.12131437754409283No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA32970.11805593351914818No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG29260.10477150787898927No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27960.10011658784335406No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66750.051.9415171
GTATCAA116100.041.169181
TATCAAC150200.031.8864152
ATCAACG151000.031.396323
TCAACGC152550.031.0773144
CAACGCA154500.030.653755
AACGCAG159300.029.7556576
CGCAGAG176550.026.5255268
ACGCAGA179500.026.2218917
GTGGTAT31400.026.0517651
TGGTATC31050.025.4268552
GCAGAGT197250.023.4524929
GAGTACT123000.022.82072312-13
GTAAGGT16800.022.3603384
TACATGG104100.022.3410852
GTACATG109050.022.2860771
TAAGGTG18150.021.744745
GTACTTT128950.021.60198814-15
AGAGTAC176100.021.33360110-11
CAGAGTA185950.021.13580910-11
Produced by FastQC (version 0.11.5)