##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938111_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2792744 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.508942101388456 34.0 31.0 34.0 31.0 34.0 2 32.73348756635051 34.0 31.0 34.0 31.0 34.0 3 32.796093376263634 34.0 31.0 34.0 31.0 34.0 4 36.25230311120532 37.0 35.0 37.0 35.0 37.0 5 36.180174051040844 37.0 37.0 37.0 35.0 37.0 6 36.23179389159909 37.0 37.0 37.0 35.0 37.0 7 36.20263511442509 37.0 37.0 37.0 35.0 37.0 8 36.195339064375396 37.0 37.0 37.0 35.0 37.0 9 37.89492162546943 39.0 38.0 39.0 35.0 39.0 10-11 37.871766620929094 39.0 38.0 39.0 35.0 39.0 12-13 37.79446737688811 39.0 38.0 39.0 35.0 39.0 14-15 39.05342630760285 40.0 38.0 41.0 36.0 41.0 16-17 38.97165834032765 40.0 38.0 41.0 36.0 41.0 18-19 39.22107020908469 40.5 39.0 41.0 36.0 41.0 20-21 39.197326715230616 41.0 39.0 41.0 36.0 41.0 22-23 39.09937735073462 40.0 39.0 41.0 35.5 41.0 24-25 38.99712200617027 40.0 38.5 41.0 35.0 41.0 26-27 38.84333401128066 40.0 38.0 41.0 35.0 41.0 28-29 38.724691199766255 40.0 38.0 41.0 35.0 41.0 30-31 38.475759682949814 40.0 38.0 41.0 34.0 41.0 32-33 38.24521277281412 40.0 38.0 41.0 34.0 41.0 34-35 38.13472663444985 40.0 38.0 41.0 34.0 41.0 36-37 38.07035464045398 40.0 38.0 41.0 33.5 41.0 38-39 37.87865751390031 40.0 37.0 41.0 33.0 41.0 40-41 37.66285004998668 40.0 37.0 41.0 33.0 41.0 42-43 37.40714562451839 40.0 37.0 41.0 32.5 41.0 44-45 37.12602426144323 40.0 36.5 41.0 31.5 41.0 46-47 36.7485218838533 40.0 36.0 41.0 31.0 41.0 48-49 36.32353788961681 39.0 35.5 41.0 30.0 41.0 50-51 35.94893534817369 39.0 35.0 40.5 29.0 41.0 52-53 35.43975799428806 38.5 35.0 40.0 27.5 41.0 54-55 35.0829866611476 38.0 34.5 40.0 27.0 41.0 56-57 34.72721076475323 38.0 34.0 40.0 26.0 41.0 58-59 34.244613004271066 37.5 33.5 40.0 25.0 41.0 60-61 33.79271712695471 37.0 33.0 40.0 23.5 41.0 62-63 33.265103604197165 36.5 32.5 39.0 22.0 40.5 64-65 32.72463731011507 36.0 32.0 39.0 20.5 40.0 66-67 32.19932671952746 35.0 31.0 39.0 18.5 40.0 68-69 31.618040536475952 35.0 31.0 38.0 14.5 40.0 70-71 31.02709467820896 35.0 30.0 37.5 9.5 39.5 72-73 30.393050347615105 34.0 29.0 37.0 7.0 39.0 74-75 29.767018566685667 34.0 29.0 36.0 4.5 39.0 76-77 28.45881810148012 32.5 27.0 35.0 2.0 37.0 78-79 29.130397558816703 34.0 29.0 35.0 2.0 37.0 80-81 28.881075386788048 34.0 29.0 35.0 2.0 37.0 82-83 28.347318622831168 34.0 27.5 35.0 2.0 36.5 84-85 27.72263641064129 33.0 26.5 35.0 2.0 36.0 86-87 27.052325060943645 33.0 25.5 35.0 2.0 35.5 88-89 26.274470556556565 32.0 24.5 35.0 2.0 35.0 90-91 25.65140592907907 32.0 22.0 34.0 2.0 35.0 92-93 24.751684902017516 31.0 18.5 34.0 2.0 35.0 94-95 23.883388523974986 31.0 6.0 34.0 2.0 35.0 96-97 22.866747900989136 31.0 2.0 34.0 2.0 35.0 98-99 21.461680340195876 30.0 2.0 34.0 2.0 35.0 100-101 17.513838540159785 23.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.15140744592028454 1101 2 0.12251429666326175 1101 3 0.124521105562728 1101 4 0.06715837678332548 1101 5 0.00648441473105521 1101 6 0.09251102684130075 1101 7 0.12306901450361352 1101 8 0.09470336320821104 1101 9 0.037711777487203335 1101 10-11 0.13801090143051908 1101 12-13 0.22277366786422448 1101 14-15 0.30191176638566475 1101 16-17 0.3739226596298906 1101 18-19 0.24006180851142744 1101 20-21 0.18173387434648447 1101 22-23 0.23572925458331895 1101 24-25 0.2948444562775734 1101 26-27 0.38189772606516215 1101 28-29 0.2969623766213374 1101 30-31 0.34968898587324304 1101 32-33 0.3354066629210095 1101 34-35 0.3176438794986183 1101 36-37 0.3730723906276694 1101 38-39 0.3733731524294299 1101 40-41 0.4365359479167594 1101 42-43 0.4949771240741043 1101 44-45 0.4607068102018985 1101 46-47 0.4992128499963826 1101 48-49 0.5732922908023994 1101 50-51 0.5683617017742435 1101 52-53 0.511539683440013 1101 54-55 0.6079581882811453 1101 56-57 0.5633684386671405 1101 58-59 0.8091434870561542 1101 60-61 0.6595114132020612 1101 62-63 0.6789158811829097 1101 64-65 0.861142039577885 1101 66-67 0.9856173991909003 1101 68-69 1.0991818486753964 1101 70-71 1.1611981828850624 1101 72-73 1.415186463446247 1101 74-75 1.3161786328257499 1101 76-77 1.3335346087287299 1101 78-79 1.4434932763235118 1101 80-81 1.501474042377275 1101 82-83 1.7135991889182343 1101 84-85 1.8036573265621598 1101 86-87 2.112909825068087 1101 88-89 2.34879433998632 1101 90-91 2.6860962408492597 1101 92-93 2.9358900550544824 1101 94-95 3.135230477442878 1101 96-97 3.323172318288357 1101 98-99 3.836023459006398 1101 100-101 3.908493814692303 1102 1 0.1394953306271276 1102 2 0.11741278999136995 1102 3 0.15005780320834816 1102 4 0.0874754150079795 1102 5 -0.06042930085882858 1102 6 -0.005934890607321108 1102 7 0.06206144076283948 1102 8 0.06441699983017912 1102 9 0.14132949501539116 1102 10-11 0.15085141316652084 1102 12-13 0.21891799967303882 1102 14-15 0.2638202174479929 1102 16-17 0.3306084600624217 1102 18-19 0.26239211723409284 1102 20-21 0.21528925508250296 1102 22-23 0.23042827167209623 1102 24-25 0.22298458992478487 1102 26-27 0.2785753904096566 1102 28-29 0.238289441378015 1102 30-31 0.3521599113233407 1102 32-33 0.3101518867083044 1102 34-35 0.34169450720379047 1102 36-37 0.24924195328302545 1102 38-39 0.29441813871473954 1102 40-41 0.38718866707515787 1102 42-43 0.5139856953830346 1102 44-45 0.38825356279602374 1102 46-47 0.5456623972769279 1102 48-49 0.6122198826100842 1102 50-51 0.7143485838278849 1102 52-53 0.7852274401231227 1102 54-55 0.8016150308209262 1102 56-57 0.782693778991927 1102 58-59 0.9142364313927445 1102 60-61 0.9201988154269216 1102 62-63 1.0783351753772692 1102 64-65 1.0018939322652756 1102 66-67 1.0668642397265629 1102 68-69 1.3976960778558372 1102 70-71 1.5266721174912554 1102 72-73 1.6693765861755487 1102 74-75 1.715450236083779 1102 76-77 1.7025159226471231 1102 78-79 1.6783499076839412 1102 80-81 1.8791528568415643 1102 82-83 1.9911643000565995 1102 84-85 2.0061995549529215 1102 86-87 2.1162020242945623 1102 88-89 2.353767411628759 1102 90-91 2.3361018119757198 1102 92-93 2.52673718035323 1102 94-95 2.733794360690016 1102 96-97 2.9417870221736386 1102 98-99 3.3966262848013855 1102 100-101 3.3712177440638804 1103 1 0.22472944141389206 1103 2 0.20468477076925495 1103 3 0.19088116264805421 1103 4 0.1713227865701441 1103 5 0.030802196939461624 1103 6 0.06156155039515454 1103 7 0.10553817571240387 1103 8 0.17946379941311363 1103 9 0.2515868151219962 1103 10-11 0.29398342975306946 1103 12-13 0.2742401295125987 1103 14-15 0.4329325632541625 1103 16-17 0.34126852183166534 1103 18-19 0.31048805355731446 1103 20-21 0.3432047054350065 1103 22-23 0.3548080925441184 1103 24-25 0.45670676166810864 1103 26-27 0.423419182516227 1103 28-29 0.4357825879268873 1103 30-31 0.49604367354703527 1103 32-33 0.5415667945694125 1103 34-35 0.5738003156006286 1103 36-37 0.517120713157567 1103 38-39 0.5970148489171052 1103 40-41 0.558181831965733 1103 42-43 0.6697506541071263 1103 44-45 0.731278051097533 1103 46-47 0.8214451121888828 1103 48-49 0.9307529830507022 1103 50-51 1.0377737186041927 1103 52-53 1.0580351823007348 1103 54-55 1.0674964841052912 1103 56-57 1.1551519528346716 1103 58-59 1.2953333750992115 1103 60-61 1.329223131598532 1103 62-63 1.4888961389943702 1103 64-65 1.5504189588254818 1103 66-67 1.5598391877701374 1103 68-69 1.6050645607282057 1103 70-71 1.518139958826982 1103 72-73 1.957023037689229 1103 74-75 1.9088575018001528 1103 76-77 1.865764211577055 1103 78-79 2.019368006720722 1103 80-81 2.198851273246394 1103 82-83 2.2728307362761164 1103 84-85 2.3762178402286196 1103 86-87 2.592965015517347 1103 88-89 2.6557126383393737 1103 90-91 2.9949047930682333 1103 92-93 3.101714747028879 1103 94-95 3.300675560717668 1103 96-97 3.6875824493027203 1103 98-99 3.8596653547637274 1103 100-101 3.730065045081002 1104 1 0.2504997335651922 1104 2 0.2343751781006631 1104 3 0.22041862259789013 1104 4 0.142961780437993 1104 5 0.029622724423454372 1104 6 0.12161150985235736 1104 7 0.1348381647276966 1104 8 0.17297728754417818 1104 9 0.21316834471321044 1104 10-11 0.19942530429764815 1104 12-13 0.2637414321647853 1104 14-15 0.41987979556899546 1104 16-17 0.39909630006131636 1104 18-19 0.34269303658971495 1104 20-21 0.328859096341354 1104 22-23 0.34558093801108214 1104 24-25 0.3634538039726536 1104 26-27 0.37040572499141433 1104 28-29 0.4008400647814341 1104 30-31 0.40929383056126767 1104 32-33 0.5379350489316508 1104 34-35 0.4858115485124941 1104 36-37 0.5011112981729227 1104 38-39 0.5336433150745634 1104 40-41 0.5616120794906081 1104 42-43 0.5697841951995812 1104 44-45 0.7042423373808191 1104 46-47 0.7847662505400166 1104 48-49 0.774087739085374 1104 50-51 0.8773544717191086 1104 52-53 0.9322189388527775 1104 54-55 1.1553931808946487 1104 56-57 1.1105322375482274 1104 58-59 1.240081972033387 1104 60-61 1.2102434851719508 1104 62-63 1.3128438081819311 1104 64-65 1.3482048274601155 1104 66-67 1.4884505781574262 1104 68-69 1.6421398780898322 1104 70-71 1.5961603970916691 1104 72-73 1.8531133202477754 1104 74-75 1.884959976545808 1104 76-77 1.8283312623171781 1104 78-79 1.9328848992726684 1104 80-81 2.0191739888674007 1104 82-83 2.1826341267774474 1104 84-85 2.3797248130024755 1104 86-87 2.571436916886409 1104 88-89 2.8701004253567888 1104 90-91 2.9538623191443385 1104 92-93 3.1580359432383993 1104 94-95 3.3392524289355556 1104 96-97 3.5087380741019984 1104 98-99 3.8487186322735027 1104 100-101 3.8425077841472337 1105 1 0.18903529163306843 1105 2 0.1969913957228897 1105 3 0.20528719982860366 1105 4 0.10466384808728435 1105 5 -0.06483870820969173 1105 6 0.03504413986684085 1105 7 0.06204446819446474 1105 8 0.07344525273001778 1105 9 0.17608964461128096 1105 10-11 0.14538163224777634 1105 12-13 0.22582896586367696 1105 14-15 0.3149353316684298 1105 16-17 0.23776818148819245 1105 18-19 0.22641579248393384 1105 20-21 0.1969796437971567 1105 22-23 0.21175605711774637 1105 24-25 0.22908276782893466 1105 26-27 0.21032457341942745 1105 28-29 0.2706973248229474 1105 30-31 0.3836826670884079 1105 32-33 0.4013927890820881 1105 34-35 0.4428978952580067 1105 36-37 0.4443735945007319 1105 38-39 0.4540702735672326 1105 40-41 0.4824890617615196 1105 42-43 0.3630773993234371 1105 44-45 0.496108220266521 1105 46-47 0.4560087596106115 1105 48-49 0.6385504331013294 1105 50-51 0.7014453946987658 1105 52-53 0.7089219035724525 1105 54-55 0.8347988737267897 1105 56-57 0.8990022585529971 1105 58-59 0.9120554563565904 1105 60-61 0.9913604278934187 1105 62-63 1.1200553138734293 1105 64-65 1.2253578159580343 1105 66-67 1.406771889180746 1105 68-69 1.5647385303688957 1105 70-71 1.7017635221871537 1105 72-73 1.7510817888115362 1105 74-75 1.5482494278599574 1105 76-77 1.5915331219558304 1105 78-79 1.7383400273422005 1105 80-81 1.9088470243420268 1105 82-83 1.9719600861139135 1105 84-85 2.136420679505868 1105 86-87 2.129597005951002 1105 88-89 2.1270227139909466 1105 90-91 2.3632957388941485 1105 92-93 2.7835072577481412 1105 94-95 2.982268559749574 1105 96-97 3.3082960416930796 1105 98-99 3.517521971725717 1105 100-101 3.4678195063108284 1106 1 0.10878184396050017 1106 2 0.0868229522498325 1106 3 -0.0010372989994635873 1106 4 0.06475120260631684 1106 5 -0.040418243666991316 1106 6 0.009234437208640145 1106 7 0.08554502588250301 1106 8 0.09871177368859207 1106 9 0.19076316999092313 1106 10-11 0.12042406694435215 1106 12-13 0.1149836332993317 1106 14-15 0.09200518070289831 1106 16-17 0.017761789908554704 1106 18-19 -0.0617936772523322 1106 20-21 0.014486637609543607 1106 22-23 -0.029895766196837315 1106 24-25 6.511987591508728E-4 1106 26-27 -0.13070319903448535 1106 28-29 0.0405903471873188 1106 30-31 0.12304007156198793 1106 32-33 0.22397311755242555 1106 34-35 0.1580094973377797 1106 36-37 0.19507466081901015 1106 38-39 0.19042259525505756 1106 40-41 0.24302923376270513 1106 42-43 0.18986463855372904 1106 44-45 0.25958973509141003 1106 46-47 0.4553213204566333 1106 48-49 0.3382048013254746 1106 50-51 0.5151310678894774 1106 52-53 0.5796568942034099 1106 54-55 0.7482783224977325 1106 56-57 0.6773173813059046 1106 58-59 0.8907162694968136 1106 60-61 0.7587952123878736 1106 62-63 0.7669007572134419 1106 64-65 0.9061304292847581 1106 66-67 1.0400906868721478 1106 68-69 0.9951382282854375 1106 70-71 0.9129206091038462 1106 72-73 1.2966653860429638 1106 74-75 1.422424618098102 1106 76-77 1.3964466378169682 1106 78-79 1.3769359077771632 1106 80-81 1.301713995084949 1106 82-83 1.8100238038036132 1106 84-85 1.763797813768896 1106 86-87 1.8790375595714046 1106 88-89 2.122593446007045 1106 90-91 2.1828350850598746 1106 92-93 2.0812579404251146 1106 94-95 2.4618919928454694 1106 96-97 2.800836634524547 1106 98-99 3.1566324607738707 1106 100-101 2.9650446200317084 1107 1 0.16850926252959653 1107 2 0.14947761713995078 1107 3 0.14798169797534655 1107 4 0.07082900093786293 1107 5 2.1882290723596043E-4 1107 6 0.07913211637147555 1107 7 0.14752263477543437 1107 8 0.14857376391911004 1107 9 0.15642420908768884 1107 10-11 0.17489345036364767 1107 12-13 0.21399507728758493 1107 14-15 0.37591609144173077 1107 16-17 0.364397112870833 1107 18-19 0.27058277787550367 1107 20-21 0.23977702067303852 1107 22-23 0.24885912468320015 1107 24-25 0.3693353171557092 1107 26-27 0.3213556822782593 1107 28-29 0.2780226915600963 1107 30-31 0.35823271983610994 1107 32-33 0.3781212920141357 1107 34-35 0.4250351997655244 1107 36-37 0.5040686972435395 1107 38-39 0.46117860209243844 1107 40-41 0.5493161884270847 1107 42-43 0.5238086596438407 1107 44-45 0.5567592069763094 1107 46-47 0.7444181631654772 1107 48-49 0.7367306411062131 1107 50-51 0.6654908270619941 1107 52-53 0.697743127321985 1107 54-55 0.718405118438028 1107 56-57 0.7393671647679199 1107 58-59 0.8600570693880769 1107 60-61 0.8662885603341195 1107 62-63 1.0509584443261915 1107 64-65 1.0834574915927533 1107 66-67 1.2253613065254498 1107 68-69 1.3728230365880698 1107 70-71 1.4182009177111645 1107 72-73 1.322753315942112 1107 74-75 1.3635959192620923 1107 76-77 1.404613286683908 1107 78-79 1.4014101542346857 1107 80-81 1.6389907536458068 1107 82-83 1.9318312035030942 1107 84-85 1.9178178444605791 1107 86-87 2.0339421749173603 1107 88-89 2.3668193377020756 1107 90-91 2.424325125112116 1107 92-93 2.528257891309284 1107 94-95 2.6865626455091736 1107 96-97 2.9275668252824296 1107 98-99 3.044974761553501 1107 100-101 2.8865817256130377 1108 1 0.2174427156613561 1108 2 0.2221672732125768 1108 3 0.17518652936195167 1108 4 0.08840296667928271 1108 5 -0.01039494100428584 1108 6 0.05482681311538329 1108 7 0.15465861832272765 1108 8 0.14253569093344254 1108 9 0.19129135393284002 1108 10-11 0.20078499507449976 1108 12-13 0.22073627714284072 1108 14-15 0.3521684455316674 1108 16-17 0.2980918719795369 1108 18-19 0.277923840093095 1108 20-21 0.2330120351728553 1108 22-23 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2316 92-93 2.531491318207305 2316 94-95 2.866327522911906 2316 96-97 3.296491744772897 2316 98-99 3.6763420710535257 2316 100-101 4.097915942939512 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 11.0 4 16.0 5 40.0 6 85.0 7 275.0 8 678.0 9 1297.0 10 2327.0 11 3829.0 12 5916.0 13 9036.0 14 11873.0 15 16416.0 16 21670.0 17 30583.0 18 30831.0 19 24404.0 20 22720.0 21 23505.0 22 25487.0 23 28187.0 24 31701.0 25 35867.0 26 41364.0 27 48532.0 28 58473.0 29 70948.0 30 85353.0 31 103161.0 32 127427.0 33 161341.0 34 212838.0 35 296468.0 36 417484.0 37 526138.0 38 300834.0 39 15623.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.143052137968965 19.061038176073424 14.949562151060034 22.846347534897575 2 15.610266386419497 20.128195532208785 40.1928949535096 24.068643127862117 3 19.072452703982595 24.15635606982118 30.279441553749326 26.4917496724469 4 12.797137667056852 17.012969946148637 36.979224667855725 33.21066771893879 5 13.39519503500334 37.54825327448298 34.43272647491546 14.623825215598213 6 32.10460110364613 36.438834507865565 17.075613473921518 14.380950914566789 7 28.315090821070605 33.39106627746761 20.2940190722816 17.99982382918019 8 26.551133442091828 34.80727832101326 19.51361605768115 19.12797217921376 9 26.348136456474347 16.618995511224803 20.225770783143744 36.8070972491571 10-11 24.91767952952365 26.765074063358473 27.00680764151673 21.310438765601145 12-13 25.942943570910902 24.03494555891983 27.621704674685542 22.400406195483725 14-15 22.547054080144832 25.469914177597374 26.217906116708157 25.76512562554964 16-17 21.743511353620555 28.576362276019978 27.25656688485713 22.423559485502338 18-19 21.70361837676493 26.792126310180954 29.937545295952656 21.56671001710146 20-21 22.28969071279 25.97173246097745 30.322650411208475 21.415926415024078 22-23 21.991420953191216 25.98562448381685 30.289041081420375 21.733913481571555 24-25 21.820627780210682 26.1529672230997 30.360088644476424 21.666316352213194 26-27 21.404888746129473 26.51541927044447 30.061283896558628 22.018408086867435 28-29 21.647956275261894 26.91558911235688 29.918263184881965 21.51819142749926 30-31 21.670389409125935 26.391319791574162 29.8553322467079 22.082958552592004 32-33 21.30286556877394 26.40949188325174 29.899016880888475 22.388625667085847 34-35 22.159865321987454 26.613554177389076 29.604041343084432 21.622539157539038 36-37 22.29549011277359 26.11427678289925 29.610385146091474 21.97984795823569 38-39 21.762279136332037 26.499969651192835 29.611232675441617 22.126518537033512 40-41 22.166888425615316 26.27178354177958 29.719799466167036 21.84152856643807 42-43 21.94765125713543 26.366111755477643 29.418362023061835 22.26787496432509 44-45 21.966320137733074 26.58245865572747 29.621044256204147 21.830176950335307 46-47 22.409537000169006 26.908373986301644 28.777503415995163 21.904585597534183 48-49 22.106161538615783 26.983640462570147 28.637945332619104 22.27225266619497 50-51 22.30233061104061 27.16903160475862 28.59893352201276 21.929704262188014 52-53 22.59992322962649 26.86399111411572 28.560226071562596 21.975859584695197 54-55 22.120824536728033 27.103773206566732 28.624374450361366 22.15102780634387 56-57 22.212305645794412 27.34120671705322 28.268252443610535 22.17823519354183 58-59 22.104997808606875 26.961959277327246 28.381799405888973 22.551243508176906 60-61 21.817735531792387 27.151002741389828 28.630479557023484 22.400782169794294 62-63 21.9476973184796 27.139365441300743 28.75036165147969 22.162575588739962 64-65 21.74178872105714 27.24941849306632 28.490921473647425 22.517871312229122 66-67 21.606646513239227 27.331359765535133 28.41060355546564 22.651390165760002 68-69 22.241426971345415 26.90959540403737 28.536991999174994 22.311985625442222 70-71 22.024109621218415 27.067661769213363 28.103632126682577 22.80459648288565 72-73 21.907344449889116 27.237877189850906 28.10106126900753 22.753717091252447 74-75 22.03298977636332 27.656688189107197 27.700480244519373 22.609841790010112 76-77 22.072699824975007 27.24916784352594 28.20437533837688 22.473756993122176 78-79 22.045414832150744 27.49949512021152 28.04256315652276 22.412526891114975 80-81 22.257440490843678 27.44544117475922 27.794882628813795 22.502235705583313 82-83 22.183460200624346 27.58316199266512 27.85198959280004 22.3813882139105 84-85 22.06320982862148 27.746690875717224 27.709396142224175 22.480703153437123 86-87 22.289782298838187 27.67801720117229 27.50939651231971 22.52280398766981 88-89 22.11899837579098 27.794151558467227 27.295555912034903 22.791294153706893 90-91 22.030779962193765 27.73684310412184 27.523426581314382 22.708950352370017 92-93 22.16175202596443 27.483471453165777 27.58802811858158 22.766748402288215 94-95 21.928254075561526 27.321802499620446 27.719583320204073 23.030360104613955 96-97 22.184103585614572 26.98683695563824 27.695495074197662 23.133564384549526 98-99 21.826830529400475 27.372988716473834 27.367044741659097 23.433136012466594 100-101 22.260114067025118 26.699099523622642 27.611786114301918 23.42900029505032 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1165.0 1 790.5 2 587.0 3 803.5 4 674.5 5 627.0 6 1260.5 7 2788.0 8 4575.0 9 5246.0 10 4335.0 11 3199.0 12 2971.5 13 3159.0 14 3380.0 15 3508.5 16 3724.0 17 4123.5 18 4536.5 19 5080.5 20 5749.5 21 6716.5 22 8165.0 23 10021.5 24 12358.5 25 15367.0 26 19406.5 27 24099.5 28 28037.0 29 31836.0 30 36151.0 31 42397.0 32 51381.0 33 54842.0 34 54809.0 35 58255.5 36 63427.0 37 68280.0 38 71760.0 39 74752.0 40 78551.0 41 82824.0 42 85227.5 43 87639.5 44 90858.0 45 96165.5 46 99675.5 47 101666.0 48 102845.0 49 100441.0 50 97518.0 51 94823.5 52 93844.5 53 95022.0 54 96576.0 55 92024.0 56 83917.5 57 75821.0 58 69281.5 59 61553.5 60 49660.5 61 40926.5 62 34219.5 63 25080.0 64 16689.0 65 11883.5 66 8814.5 67 6778.5 68 5160.0 69 3926.0 70 2958.5 71 1988.5 72 1394.0 73 1093.5 74 773.5 75 489.5 76 332.5 77 220.5 78 133.5 79 70.5 80 42.5 81 23.5 82 16.5 83 10.5 84 6.0 85 6.0 86 3.5 87 2.0 88 2.0 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.016005763507145658 3 0.002399074172211989 4 0.0020410034002400507 5 0.118020126441951 6 3.580707719719387E-5 7 0.0 8 4.296849263663265E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 1.7903538598596937E-5 18-19 0.0 20-21 0.0 22-23 3.4016723337334175E-4 24-25 5.37106157957908E-4 26-27 0.005979781891931377 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010956965622341325 36-37 8.772733913312498E-4 38-39 0.007609003904403697 40-41 0.010652605466165177 42-43 0.005908167737536989 44-45 0.003437479410930612 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 5.371061579579081E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 5.371061579579081E-5 68-69 0.001145826470310204 70-71 0.0 72-73 8.951769299298468E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 5.371061579579081E-5 82-83 0.00222003878622602 84-85 0.004457981111050637 86-87 0.003061505100360076 88-89 0.0 90-91 0.002971987407367091 92-93 0.0 94-95 0.0 96-97 3.580707719719387E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2792744.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.703328192212865 #Duplication Level Percentage of deduplicated Percentage of total 1 70.16538358623711 33.47122320946775 2 15.493087231002297 14.781436497821701 3 5.961094583212896 8.530921538634814 4 2.837327306222802 5.413998227098942 5 1.5226382173990594 3.631745530129665 6 0.9749724015406016 2.790565706942476 7 0.6326368374370531 2.1125217877920357 8 0.4215681916531118 1.608816464146087 9 0.32146567021003125 1.380148413170291 >10 1.5076111011156494 12.815349927693328 >50 0.10096529188968947 3.3056861472626853 >100 0.053967558464281244 4.78814453964732 >500 0.0036034756036171143 1.2108407953413207 >1k 0.003453330786730194 2.971826108636962 >5k 7.507240840717814E-5 0.2691632728298878 >10k+ 1.5014481681435628E-4 0.9176118333848078 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13982 0.5006545533711647 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11641 0.4168301856525339 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7516 0.26912599221410916 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3568 0.12775965143958773 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3567 0.12772384436239054 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3509 0.1256470338849533 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3388 0.12131437754409283 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3297 0.11805593351914818 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2926 0.10477150787898927 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2796 0.10011658784335406 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.580707719719387E-5 2 3.580707719719387E-5 0.0 0.0 0.0 3.580707719719387E-5 3 3.580707719719387E-5 0.0 0.0 0.0 3.580707719719387E-5 4 3.580707719719387E-5 0.0 0.0 0.0 3.580707719719387E-5 5 3.580707719719387E-5 0.0 0.0 0.0 3.580707719719387E-5 6 1.0742123159158162E-4 0.0 0.0 0.0 3.580707719719387E-5 7 1.7903538598596937E-4 0.0 0.0 0.0 3.580707719719387E-5 8 2.1484246318316324E-4 0.0 0.0 0.0 3.580707719719387E-5 9 2.1484246318316324E-4 0.0 0.0 0.0 3.580707719719387E-5 10-11 2.506495403803571E-4 0.0 0.0 0.0 3.580707719719387E-5 12-13 2.86456617577551E-4 0.0 0.0 0.0 3.580707719719387E-5 14-15 3.2226369477474483E-4 0.0 0.0 0.0 3.580707719719387E-5 16-17 3.2226369477474483E-4 0.0 0.0 0.0 3.580707719719387E-5 18-19 4.117813877677295E-4 0.0 0.0 0.0 3.580707719719387E-5 20-21 5.012990807607142E-4 0.0 0.0 3.580707719719387E-5 3.580707719719387E-5 22-23 5.37106157957908E-4 0.0 0.0 1.7903538598596937E-4 3.580707719719387E-5 24-25 7.877556983382652E-4 0.0 0.0 5.012990807607142E-4 3.580707719719387E-5 26-27 7.877556983382652E-4 0.0 0.0 0.0012532477019017855 3.580707719719387E-5 28-29 0.0010563087773172192 0.0 0.0 0.004332656340860459 3.580707719719387E-5 30-31 0.0011816335475073978 0.0 0.0 0.01378572472091964 5.371061579579081E-5 32-33 0.0011816335475073978 0.0 0.0 0.03473286488127805 7.161415439438775E-5 34-35 0.0012711512405003825 0.0 0.0 0.06642212820079463 7.161415439438775E-5 36-37 0.0015038972422821426 0.0 0.0 0.11771576628577485 7.161415439438775E-5 38-39 0.001647125551070918 0.0 0.0 0.19183641608396618 7.161415439438775E-5 40-41 0.001647125551070918 0.0 0.0 0.2719905583898846 7.161415439438775E-5 42-43 0.001718739705465306 0.0 0.0 0.3544542571750221 7.161415439438775E-5 44-45 0.0018619680142540813 0.0 0.0 0.4533534043936716 7.161415439438775E-5 46-47 0.0021484246318316325 0.0 0.0 0.5702993185197067 7.161415439438775E-5 48-49 0.0023274600178176015 0.0 0.0 0.6908080368268628 7.161415439438775E-5 50-51 0.0023274600178176015 0.0 0.0 0.8192659262717958 7.161415439438775E-5 52-53 0.0023274600178176015 0.0 0.0 0.938163326105078 1.432283087887755E-4 54-55 0.002470688326606377 0.0 0.0 1.0666749261658068 1.432283087887755E-4 56-57 0.002542302481000765 0.0 0.0 1.2164201230044718 1.432283087887755E-4 58-59 0.002542302481000765 0.0 0.0 1.3753677386828151 1.432283087887755E-4 60-61 0.002542302481000765 0.0 0.0 1.5446456961325492 1.432283087887755E-4 62-63 0.0025781095581979586 0.0 0.0 1.7080512929219434 1.7903538598596937E-4 64-65 0.0026139166353951527 0.0 0.0 1.877257636217283 1.7903538598596937E-4 66-67 0.002757144944183928 0.0 0.0 2.07494850942299 1.9693892458456632E-4 68-69 0.002757144944183928 0.0 0.0 2.2895761301429705 2.1484246318316324E-4 70-71 0.002792952021381122 0.0 0.0 2.5083752753564235 2.1484246318316324E-4 72-73 0.0028645661757755094 0.0 0.0 2.734317932470717 2.86456617577551E-4 74-75 0.0029003732529727035 0.0 0.0 2.964270982231096 2.86456617577551E-4 76-77 0.0029003732529727035 0.0 0.0 3.209227197337099 2.86456617577551E-4 78-79 0.0029361803301698975 0.0 0.0 3.4839749006711678 2.86456617577551E-4 80-81 0.003240540486346045 0.0 0.0 3.7676743733045353 2.86456617577551E-4 82-83 0.0032584440249446424 0.0 0.0 4.062169679712856 3.5807077197193873E-4 84-85 0.003276347563543239 0.0 0.0 4.364739482029144 3.5807077197193873E-4 86-87 0.003294251102141836 0.0 0.0 4.672161143305653 3.7597431057053565E-4 88-89 0.003294251102141836 0.0 0.0 4.994729198236573 3.938778491691326E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6675 0.0 51.941517 1 GTATCAA 11610 0.0 41.16918 1 TATCAAC 15020 0.0 31.886415 2 ATCAACG 15100 0.0 31.39632 3 TCAACGC 15255 0.0 31.077314 4 CAACGCA 15450 0.0 30.65375 5 AACGCAG 15930 0.0 29.755657 6 CGCAGAG 17655 0.0 26.525526 8 ACGCAGA 17950 0.0 26.221891 7 GTGGTAT 3140 0.0 26.051765 1 TGGTATC 3105 0.0 25.426855 2 GCAGAGT 19725 0.0 23.452492 9 GAGTACT 12300 0.0 22.820723 12-13 GTAAGGT 1680 0.0 22.360338 4 TACATGG 10410 0.0 22.341085 2 GTACATG 10905 0.0 22.286077 1 TAAGGTG 1815 0.0 21.74474 5 GTACTTT 12895 0.0 21.601988 14-15 AGAGTAC 17610 0.0 21.333601 10-11 CAGAGTA 18595 0.0 21.135809 10-11 >>END_MODULE