FastQCFastQC Report
Thu 26 May 2016
SRR938110_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938110_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2770316
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146860.530120029628389No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113880.41107223869045983No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72690.26238883939593893No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA36630.13222318320364898No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA34680.12518427500689452No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA33300.12020289382149907No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA32580.11760391233346665No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC31190.11258643418295963No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63700.054.0128021
GTATCAA111850.041.7683751
ATCAACG142950.032.3151323
TATCAAC144050.032.2663232
TCAACGC146050.031.564144
CAACGCA147100.031.3388395
AACGCAG152600.030.2683326
ACGCAGA172000.026.6246667
CGCAGAG171750.026.60818
TGGTATC27950.025.5054662
GTGGTAT30900.024.2461091
GCAGAGT187500.024.0685569
GAGTACT120150.022.79288512-13
GTAAGGT16750.022.4148654
TACATGG102150.022.0993292
GTACATG104850.021.9826261
GTACTTT125350.021.75417914-15
CAGAGTA181050.021.3572710-11
AGAGTAC172100.021.14305510-11
ACATGGG104600.020.8093073
Produced by FastQC (version 0.11.5)