##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938110_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2770316 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.848409712105045 33.0 31.0 34.0 30.0 34.0 2 32.048060582258486 34.0 31.0 34.0 30.0 34.0 3 32.12285963045371 34.0 31.0 34.0 30.0 34.0 4 35.652354821616015 37.0 35.0 37.0 33.0 37.0 5 35.664324936216666 37.0 35.0 37.0 35.0 37.0 6 35.68911813670354 37.0 35.0 37.0 33.0 37.0 7 35.62792042496235 37.0 35.0 37.0 33.0 37.0 8 35.59839635622795 37.0 35.0 37.0 33.0 37.0 9 37.238265237611884 39.0 37.0 39.0 34.0 39.0 10-11 37.15347238365587 39.0 37.0 39.0 34.0 39.0 12-13 37.0503698856015 39.0 37.0 39.0 33.0 39.0 14-15 38.22948699715123 40.0 38.0 41.0 33.0 41.0 16-17 38.08198866122132 40.0 38.0 41.0 33.0 41.0 18-19 38.2439826359159 40.0 38.0 41.0 33.5 41.0 20-21 38.229923770429075 40.0 38.0 41.0 34.0 41.0 22-23 38.14394567262363 40.0 38.0 41.0 33.0 41.0 24-25 38.03657759620202 40.0 38.0 41.0 33.0 41.0 26-27 37.85865096256167 40.0 38.0 41.0 33.0 41.0 28-29 37.648787358554046 40.0 37.5 41.0 32.0 41.0 30-31 37.4214407309491 40.0 37.0 41.0 32.0 41.0 32-33 37.146517220418175 40.0 36.5 41.0 31.0 41.0 34-35 36.90429196524873 39.5 36.0 41.0 30.5 41.0 36-37 36.62975270691142 39.0 35.5 41.0 30.0 41.0 38-39 36.327218266797004 39.0 35.0 40.5 30.0 41.0 40-41 36.098766891574826 39.0 35.0 40.0 30.0 41.0 42-43 35.90183809356044 39.0 35.0 40.0 28.5 41.0 44-45 35.61534478377196 39.0 35.0 40.0 27.0 41.0 46-47 35.20476581010975 38.5 35.0 40.0 25.5 41.0 48-49 34.78912333466651 38.0 34.0 40.0 24.0 41.0 50-51 34.07553560676833 37.5 33.5 39.5 23.0 40.5 52-53 34.1949227091783 38.0 34.0 40.0 23.0 40.5 54-55 34.29627197763721 38.0 34.0 40.0 23.0 41.0 56-57 33.9893055521464 38.0 34.0 40.0 22.0 41.0 58-59 33.66697012182004 38.0 33.0 40.0 20.5 41.0 60-61 33.24892160316729 37.0 33.0 40.0 18.5 41.0 62-63 32.707010499885214 36.5 32.0 39.5 15.0 41.0 64-65 32.17820512172619 36.0 31.5 39.0 10.0 40.5 66-67 31.64842999859944 35.0 31.0 39.0 7.5 40.0 68-69 31.04509972869521 35.0 30.5 38.0 4.0 40.0 70-71 30.482873975387648 35.0 29.5 37.5 2.0 40.0 72-73 29.829544174743965 34.0 29.0 37.0 2.0 39.0 74-75 29.13907005554601 34.0 28.0 36.0 2.0 39.0 76-77 28.458318112446378 34.0 26.5 36.0 2.0 38.5 78-79 27.714038578992433 33.0 26.0 35.0 2.0 37.5 80-81 27.050052773762992 33.0 25.0 35.0 2.0 37.0 82-83 26.29469833044317 32.0 23.5 35.0 2.0 36.0 84-85 25.61311056211638 32.0 20.5 35.0 2.0 36.0 86-87 24.90861818651735 31.0 18.5 34.0 2.0 35.0 88-89 24.054634922514254 31.0 10.0 34.0 2.0 35.0 90-91 23.19426303714089 30.0 4.0 34.0 2.0 35.0 92-93 22.274742123281243 29.5 2.0 34.0 2.0 35.0 94-95 21.28088257801637 29.0 2.0 34.0 2.0 35.0 96-97 20.040333846391533 28.0 2.0 34.0 2.0 35.0 98-99 18.49834477366481 25.5 2.0 33.0 2.0 35.0 100-101 14.850249935386433 9.5 2.0 29.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4022358114755882 1101 2 0.38140305664084906 1101 3 0.28915295750989145 1101 4 0.29104424181742417 1101 5 0.28133063515649326 1101 6 0.283353003232385 1101 7 0.24599014683919052 1101 8 0.14339718559527626 1101 9 0.28864974931367016 1101 10-11 0.39110681686413074 1101 12-13 0.4271761885076586 1101 14-15 0.5570360784632484 1101 16-17 0.5669190727656712 1101 18-19 0.4441804566133527 1101 20-21 0.5977068894664157 1101 22-23 0.5516424633147992 1101 24-25 0.5322240994920406 1101 26-27 0.5315445554408385 1101 28-29 0.6311687779808679 1101 30-31 0.6950676580020101 1101 32-33 0.7459397555773535 1101 34-35 0.8765595845024805 1101 36-37 0.9099075613759666 1101 38-39 1.0064741441463383 1101 40-41 1.2278459310798908 1101 42-43 1.3499032819723666 1101 44-45 1.0884383612621562 1101 46-47 1.3181940377247088 1101 48-49 1.3239836702244148 1101 50-51 1.4052520059979159 1101 52-53 1.359188438843411 1101 54-55 1.4779721297339492 1101 56-57 1.4508742464192181 1101 58-59 1.4852326371920057 1101 60-61 1.5148471711202092 1101 62-63 1.3371655351021872 1101 64-65 1.4675426566349472 1101 66-67 1.5501186305428938 1101 68-69 1.6906620798514034 1101 70-71 2.0247156792166017 1101 72-73 2.12902745475213 1101 74-75 2.2528734706542224 1101 76-77 2.3482167817564594 1101 78-79 2.5762949079066466 1101 80-81 2.785420741392045 1101 82-83 2.9345117920248143 1101 84-85 3.005501644079331 1101 86-87 3.2982711841610417 1101 88-89 3.8884653653235794 1101 90-91 4.090276081297127 1101 92-93 4.28236591758882 1101 94-95 4.505869184006709 1101 96-97 4.531476455205553 1101 98-99 4.7949218234082664 1101 100-101 4.923326800690349 1102 1 0.3887793277326246 1102 2 0.1904405065067678 1102 3 0.18800469294735933 1102 4 0.15648457240516223 1102 5 0.2123591360834638 1102 6 0.35777624387560536 1102 7 0.14939094347185033 1102 8 0.19867929560720654 1102 9 0.2993620158097272 1102 10-11 0.37483428043219646 1102 12-13 0.5062028406625316 1102 14-15 0.6267661563590607 1102 16-17 0.5938792414773175 1102 18-19 0.4153653141845126 1102 20-21 0.3740617110716187 1102 22-23 0.48123752847833146 1102 24-25 0.27905352080845347 1102 26-27 0.46067949478726433 1102 28-29 0.49587214928535417 1102 30-31 0.37893098874788933 1102 32-33 0.5103211421702056 1102 34-35 0.4302025716578939 1102 36-37 0.47552458647910356 1102 38-39 0.3966288814784136 1102 40-41 0.6406419249706019 1102 42-43 0.6839969168009503 1102 44-45 0.7099791918728116 1102 46-47 0.6996942408277889 1102 48-49 0.8265380489649843 1102 50-51 0.9670217866707063 1102 52-53 0.9068572840016884 1102 54-55 0.9575818031600463 1102 56-57 1.0063948604348099 1102 58-59 0.9914764635645312 1102 60-61 1.0348896039885247 1102 62-63 1.1278791762908682 1102 64-65 1.1611259083997396 1102 66-67 1.3042216918352345 1102 68-69 1.5127027783330753 1102 70-71 1.589425286595425 1102 72-73 1.901807041910228 1102 74-75 1.7833935420279872 1102 76-77 1.9397365130746813 1102 78-79 2.1804896326011907 1102 80-81 2.2760750709675364 1102 82-83 2.5919268461839096 1102 84-85 2.5600643660672127 1102 86-87 2.888062041386977 1102 88-89 3.0221879881819937 1102 90-91 3.37347516676477 1102 92-93 3.671631325257536 1102 94-95 4.013692959436366 1102 96-97 4.206715398490697 1102 98-99 4.307328596680797 1102 100-101 4.336179298238131 1103 1 0.6068933344198335 1103 2 0.5086580471039994 1103 3 0.459817576060388 1103 4 0.4116792548972157 1103 5 0.3479672110168721 1103 6 0.3762935544765398 1103 7 0.428510300399374 1103 8 0.45467686993301015 1103 9 0.47452842512406335 1103 10-11 0.5787866943654336 1103 12-13 0.6408439665928114 1103 14-15 0.7865749368568871 1103 16-17 0.7821381047153082 1103 18-19 0.7333342982871756 1103 20-21 0.7891644978297165 1103 22-23 0.7115829854388167 1103 24-25 0.7648994391056121 1103 26-27 0.8401330539581338 1103 28-29 0.8220925172003533 1103 30-31 0.7550393629373886 1103 32-33 0.9240312219217515 1103 34-35 0.872195810921248 1103 36-37 1.0598720105443462 1103 38-39 1.1029054715876967 1103 40-41 1.075625278109392 1103 42-43 1.2138770641440502 1103 44-45 1.1895622771437928 1103 46-47 1.2749281903045073 1103 48-49 1.385961631135487 1103 50-51 1.3879146055523819 1103 52-53 1.4279683779823245 1103 54-55 1.46303532042716 1103 56-57 1.5841073579541884 1103 58-59 1.6484516011946582 1103 60-61 1.654923514407777 1103 62-63 1.7367884293524156 1103 64-65 1.8394528889650914 1103 66-67 1.9469635852368192 1103 68-69 2.1094496630257957 1103 70-71 2.3270858155473597 1103 72-73 2.385878573207986 1103 74-75 2.4468129638668046 1103 76-77 2.66300240902844 1103 78-79 2.796212594991381 1103 80-81 2.9838028752184798 1103 82-83 3.04735231560484 1103 84-85 3.0352606021608963 1103 86-87 3.0821955349033594 1103 88-89 3.3726622319970367 1103 90-91 3.539638593005847 1103 92-93 3.8706144870621806 1103 94-95 4.2835678925287155 1103 96-97 4.614498567094817 1103 98-99 5.077738395677159 1103 100-101 4.699371728205483 1104 1 0.3249402621446329 1104 2 0.2837303194264038 1104 3 0.24626815244025124 1104 4 0.22987317351764602 1104 5 0.2683784401715741 1104 6 0.2960446556491618 1104 7 0.2777572148856393 1104 8 0.251489117303656 1104 9 0.42148507324239404 1104 10-11 0.3450396928214161 1104 12-13 0.3937899001325036 1104 14-15 0.5014955200430862 1104 16-17 0.5154170809161656 1104 18-19 0.5480003994382088 1104 20-21 0.5644195194790385 1104 22-23 0.5152093004785172 1104 24-25 0.5365719623845706 1104 26-27 0.6569254147484784 1104 28-29 0.6367530423384622 1104 30-31 0.6823321115304353 1104 32-33 0.6398398301345338 1104 34-35 0.5880876995851736 1104 36-37 0.7144630117948125 1104 38-39 0.7720651556472049 1104 40-41 0.7175819334225721 1104 42-43 0.6113406617732053 1104 44-45 0.8208656899514963 1104 46-47 0.7402894583833799 1104 48-49 0.8937005974650063 1104 50-51 0.9013430836533942 1104 52-53 0.8791831213922023 1104 54-55 0.8778628021846018 1104 56-57 1.0559689980070885 1104 58-59 1.1394114452677613 1104 60-61 1.2792585298983 1104 62-63 1.197195859623477 1104 64-65 1.3098277121515167 1104 66-67 1.3551682026072136 1104 68-69 1.3920484735722987 1104 70-71 1.7594628087021782 1104 72-73 1.7798021221135798 1104 74-75 2.0304022541806575 1104 76-77 1.986226430923825 1104 78-79 2.205822187748179 1104 80-81 2.2916430371890613 1104 82-83 2.5969781111759787 1104 84-85 2.7718125180789386 1104 86-87 2.819917694050684 1104 88-89 3.0724261328173945 1104 90-91 3.3900773352875717 1104 92-93 3.594300742442133 1104 94-95 3.5927331669908646 1104 96-97 3.8146250886268653 1104 98-99 3.9183901560424275 1104 100-101 3.8671958672331126 1105 1 0.2543365450475541 1105 2 0.2899091034539367 1105 3 0.15988391483602982 1105 4 0.06822820192760304 1105 5 0.0976080520404281 1105 6 0.18444139276204652 1105 7 0.21125503555131786 1105 8 0.22230312487673132 1105 9 0.35632286787834744 1105 10-11 0.2757008849478595 1105 12-13 0.18727477144695825 1105 14-15 0.35700284308681773 1105 16-17 0.4412677146264059 1105 18-19 0.2695142834137698 1105 20-21 0.3494197542269717 1105 22-23 0.2803027738308046 1105 24-25 0.36347907098879517 1105 26-27 0.22242609387971157 1105 28-29 0.3465353982469068 1105 30-31 0.37028714037712973 1105 32-33 0.4945827210935789 1105 34-35 0.4213974216436185 1105 36-37 0.5317023269422805 1105 38-39 0.6058442370949209 1105 40-41 0.49823919488591883 1105 42-43 0.5230918158595657 1105 44-45 0.41291690649173063 1105 46-47 0.3895653974843114 1105 48-49 0.6088398466646794 1105 50-51 0.6950557423539223 1105 52-53 0.5528342215411328 1105 54-55 0.7119620688975701 1105 56-57 0.8393014305634523 1105 58-59 0.7909479250969085 1105 60-61 0.8110530905156779 1105 62-63 0.7762177468658535 1105 64-65 0.8786297388450919 1105 66-67 1.1081742045113963 1105 68-69 1.227636205972754 1105 70-71 1.246239129950908 1105 72-73 1.3459094608799624 1105 74-75 1.505668556605876 1105 76-77 1.6040274487471962 1105 78-79 1.7104760655656968 1105 80-81 1.8638332135884852 1105 82-83 1.9568812257897434 1105 84-85 2.053715035450285 1105 86-87 2.4429242473395583 1105 88-89 2.488712835384117 1105 90-91 2.522750726663695 1105 92-93 2.771540483411435 1105 94-95 3.074144406066896 1105 96-97 3.299583751153232 1105 98-99 3.6938439292606375 1105 100-101 3.507035758222722 1106 1 0.17242078151322104 1106 2 0.1263008551616096 1106 3 0.060461724458058086 1106 4 0.17628524425092706 1106 5 0.17120595622510137 1106 6 -0.013572216962472794 1106 7 0.1344588671943754 1106 8 0.15726138466251172 1106 9 0.12145547490980846 1106 10-11 0.1574080792167436 1106 12-13 0.20954768612335783 1106 14-15 0.28532831289488314 1106 16-17 0.10975718757250519 1106 18-19 0.13767291099004808 1106 20-21 0.19094289771798856 1106 22-23 0.12231722289372016 1106 24-25 -0.008361349798121864 1106 26-27 0.13431164228612857 1106 28-29 0.09257786760999664 1106 30-31 0.16257118472287857 1106 32-33 0.08540340635644128 1106 34-35 0.2034791153146287 1106 36-37 0.2839809171701049 1106 38-39 -0.011995684412177354 1106 40-41 0.41067503189399446 1106 42-43 0.3983562179996696 1106 44-45 0.4290949959635171 1106 46-47 0.6930158849543417 1106 48-49 0.648705718595977 1106 50-51 0.6874038310665114 1106 52-53 0.6979490163566311 1106 54-55 0.2340566505253392 1106 56-57 0.5975041576751465 1106 58-59 0.7276319822014372 1106 60-61 0.8121311948551337 1106 62-63 0.6747122363351039 1106 64-65 0.8649467962891535 1106 66-67 1.0523113440756582 1106 68-69 0.8685426439935213 1106 70-71 1.036744984230797 1106 72-73 1.3533507467337351 1106 74-75 1.4224506850246499 1106 76-77 1.571723530745075 1106 78-79 1.9050377511385683 1106 80-81 2.0845081692490623 1106 82-83 2.024608572088539 1106 84-85 2.132371245183659 1106 86-87 1.9411505386659016 1106 88-89 2.1975783999446357 1106 90-91 2.3578731240292683 1106 92-93 2.753422280628964 1106 94-95 2.766486286733219 1106 96-97 2.9340460789886897 1106 98-99 3.269599755597728 1106 100-101 3.280629497348757 1107 1 0.34354396584929603 1107 2 0.20045857036618742 1107 3 0.06556361446296677 1107 4 0.08571635100043551 1107 5 0.15642643709153958 1107 6 0.21891553081616877 1107 7 0.12993633433950436 1107 8 0.11904745999076027 1107 9 0.23652881088374755 1107 10-11 0.21055227411721233 1107 12-13 0.27055162929906373 1107 14-15 0.3492142623016363 1107 16-17 0.4658342302498397 1107 18-19 0.2976198229573157 1107 20-21 0.36126207621319395 1107 22-23 0.43192745741512795 1107 24-25 0.47847009865387946 1107 26-27 0.5200748270951507 1107 28-29 0.5859477753266802 1107 30-31 0.5799891436121669 1107 32-33 0.5670974939576041 1107 34-35 0.6449463077380599 1107 36-37 0.7165360595559918 1107 38-39 0.664900380097464 1107 40-41 0.7473288305259942 1107 42-43 0.8524894583205551 1107 44-45 0.7386402006007344 1107 46-47 0.9008573066258379 1107 48-49 1.0290389080753286 1107 50-51 0.8479921741641832 1107 52-53 0.9990048652035313 1107 54-55 1.047444530137284 1107 56-57 1.2214599846483551 1107 58-59 1.1623094635349034 1107 60-61 1.2368502361964104 1107 62-63 1.2654880428534696 1107 64-65 1.36607299564713 1107 66-67 1.477225475069499 1107 68-69 1.5654560892176654 1107 70-71 1.8676347704007092 1107 72-73 2.018588726550824 1107 74-75 2.074372318102398 1107 76-77 2.255920677706701 1107 78-79 2.508358413497497 1107 80-81 2.519123576602169 1107 82-83 2.6807364563116955 1107 84-85 2.7748369419197516 1107 86-87 2.9181802100285132 1107 88-89 3.1006306662361 1107 90-91 3.452902476154268 1107 92-93 3.72266018950436 1107 94-95 4.090181402988918 1107 96-97 4.161452628089204 1107 98-99 4.469108484381728 1107 100-101 4.211204184170745 1108 1 0.2607262895905471 1108 2 0.20626891485596843 1108 3 0.12324458484270195 1108 4 0.10514783240305547 1108 5 0.061886706126031754 1108 6 0.24903405715090088 1108 7 0.21068668944218416 1108 8 0.14632657274969318 1108 9 0.2550230112741758 1108 10-11 0.2597042101967375 1108 12-13 0.27436745058847123 1108 14-15 0.3935154846385629 1108 16-17 0.44577631099961224 1108 18-19 0.4251057616890108 1108 20-21 0.4024815575327523 1108 22-23 0.3404452992496303 1108 24-25 0.34063476610447907 1108 26-27 0.42760235862498774 1108 28-29 0.4684511709025614 1108 30-31 0.4478352192861408 1108 32-33 0.5228274204639263 1108 34-35 0.5492087248343225 1108 36-37 0.5067092975726908 1108 38-39 0.47196350642531826 1108 40-41 0.48833241692521057 1108 42-43 0.5256794318892091 1108 44-45 0.6905803511781201 1108 46-47 0.7665116417529063 1108 48-49 0.7821372794073156 1108 50-51 0.703475268085505 1108 52-53 0.7343061015017085 1108 54-55 0.7667895808324587 1108 56-57 0.9521830449172057 1108 58-59 0.9179622159956935 1108 60-61 1.0725419065264319 1108 62-63 1.1987364237295566 1108 64-65 1.3454441902527847 1108 66-67 1.4299110274382123 1108 68-69 1.5572773180870492 1108 70-71 1.6967281067656295 1108 72-73 1.8424814966286043 1108 74-75 1.8771345576326617 1108 76-77 2.0914351339963098 1108 78-79 2.2830357179340908 1108 80-81 2.3253726680942393 1108 82-83 2.446622043736056 1108 84-85 2.651686323829324 1108 86-87 2.8698414288739507 1108 88-89 2.9577145525933908 1108 90-91 3.041221237577709 1108 92-93 3.3252281681884597 1108 94-95 3.638128154539995 1108 96-97 3.8780310045035584 1108 98-99 4.0119665356420455 1108 100-101 3.7841703201573242 1109 1 0.199379679743803 1109 2 0.08035664103065443 1109 3 0.09067109283613206 1109 4 0.1258524105207144 1109 5 0.05898522085779234 1109 6 0.12544194855924928 1109 7 0.06394908127168009 1109 8 0.06529391797517548 1109 9 0.02908566329664808 1109 10-11 0.029259074498874327 1109 12-13 0.1403455510129561 1109 14-15 0.11134053821997014 1109 16-17 0.09736740772969199 1109 18-19 0.13071347629956875 1109 20-21 0.1931510262776186 1109 22-23 0.15751835065164244 1109 24-25 0.02587087177173686 1109 26-27 0.005793016696394204 1109 28-29 0.06390146320268286 1109 30-31 0.1066780832991725 1109 32-33 0.0953146818494588 1109 34-35 0.09706794264308627 1109 36-37 0.16312341582150225 1109 38-39 0.03222297664351714 1109 40-41 0.15978346534166832 1109 42-43 0.038577775938826164 1109 44-45 0.38024835494758236 1109 46-47 0.463443532905508 1109 48-49 0.13799231633748832 1109 50-51 0.24882640351094665 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4588.0 9 5958.0 10 7609.0 11 9820.0 12 12528.0 13 15705.0 14 18446.0 15 22941.0 16 27730.0 17 34650.0 18 33510.0 19 27956.0 20 27362.0 21 28863.0 22 31598.0 23 35339.0 24 39687.0 25 44688.0 26 50485.0 27 58363.0 28 67349.0 29 78549.0 30 93905.0 31 112890.0 32 137195.0 33 172868.0 34 224129.0 35 299885.0 36 383411.0 37 415124.0 38 213803.0 39 12417.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.86408651374024 18.661868406108646 14.624251087695175 22.84979399245594 2 15.390484843407396 19.922620921962253 40.569740837144266 24.11715339748608 3 19.005248989314943 24.298154438888314 30.004904449903464 26.69169212189328 4 12.577185100860255 16.75281012094996 37.20422084265887 33.465783935530915 5 13.426676699910518 37.493276149141785 34.39873329694296 14.68131385400474 6 32.00588265469424 36.34901872666753 17.2479478985513 14.397150720086932 7 28.195232403190918 33.44085283719318 20.470151059365644 17.89376370025026 8 26.52614040517005 34.5892779182286 19.677083058741026 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26.422377486008653 29.897575856611613 22.35338089954489 34-35 22.212746225195552 26.582525109837196 29.569486575313082 21.635242089654167 36-37 22.30056790235806 26.111988640508343 29.581822935512786 22.00562052162081 38-39 21.74401729329403 26.489220485707744 29.595629883213583 22.171132337784652 40-41 22.156406082532982 26.308184535441697 29.71350181438681 21.82190756763851 42-43 21.91127290320426 26.36903821661225 29.43143999877242 22.288248881411068 44-45 21.92292758424803 26.654088602067617 29.55204363765282 21.87094017603153 46-47 22.382277569213578 26.885910317293398 28.789056893646098 21.942755219846926 48-49 22.106745989980116 26.978706441938137 28.662942774815146 22.251604793266594 50-51 22.290518636160066 27.17325593380629 28.609821289479058 21.926404140554588 52-53 22.550257889442122 26.854366159504256 28.601494247873156 21.99388170318047 54-55 22.090471081930424 27.146902461254026 28.63213542447954 22.13049103233601 56-57 22.196431018015677 27.353001117190967 28.323363335088516 22.12720452970484 58-59 22.09029777047155 26.867159903181857 28.483208363516045 22.559333962830543 60-61 21.777716241688566 27.023473290228385 28.80771701068911 22.39109345739394 62-63 21.90685449012869 26.981676580186694 28.946389370911024 22.165079558773595 64-65 21.783562471241556 27.18501457756472 28.614303685936925 22.4171192652568 66-67 21.635686162173695 27.170480950062785 28.618145133559935 22.57568775420358 68-69 22.176481588582675 26.80105098762945 28.71865575245328 22.303811671334593 70-71 22.02070626139394 26.82510189673513 28.34354782778852 22.81064401408241 72-73 21.89685784902339 27.000010108719273 28.373261571053828 22.72987047120351 74-75 21.998590228532798 27.3864443523851 28.06959686131802 22.54536855776408 76-77 22.16416261679409 27.03182546427027 28.250000180502415 22.554011738433225 78-79 22.12116666549335 27.22369909548207 28.112963926686195 22.542170312338385 80-81 22.333941188809344 27.151192827618274 27.855581914813342 22.659284068759035 82-83 22.331748325669434 27.24677634279488 27.8803883010044 22.541087030531283 84-85 22.17774492522886 27.42279876640484 27.79722756978685 22.602228738579452 86-87 22.45823107730222 27.296562013405833 27.57040392042268 22.67480298886926 88-89 22.314741581772335 27.306544462399497 27.439814223902225 22.938899731925943 90-91 22.22052315379917 27.330612857992165 27.462099089309355 22.986764898899313 92-93 22.407060252773366 26.933748755583515 27.695588876383297 22.963602115259818 94-95 22.239955283798395 26.666945902206418 27.841432247227445 23.251666566767746 96-97 22.507013622856647 26.2828159921433 27.79932914742509 23.410841237574964 98-99 22.201572224065576 26.612887211535373 27.430266089517772 23.755274474881276 100-101 23.015819507840344 25.480287025350144 27.617613316865715 23.886280149943794 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1192.0 1 801.0 2 608.0 3 801.0 4 673.0 5 696.0 6 1293.0 7 2693.5 8 4518.0 9 5199.5 10 4207.0 11 3224.5 12 3133.0 13 3279.0 14 3382.5 15 3577.5 16 3869.5 17 4184.0 18 4569.5 19 5085.5 20 5742.0 21 6777.5 22 8208.0 23 10057.0 24 12316.0 25 15179.5 26 19279.0 27 23872.5 28 27755.0 29 31410.5 30 35526.5 31 41880.5 32 50409.5 33 53845.5 34 54141.0 35 57694.0 36 63075.0 37 68290.5 38 71656.5 39 74095.5 40 77538.0 41 81738.5 42 84033.0 43 86139.5 44 89169.0 45 93937.5 46 97531.0 47 99584.5 48 100682.0 49 98465.0 50 95625.5 51 93510.5 52 93140.0 53 93830.0 54 95051.0 55 90970.0 56 83027.5 57 75645.5 58 69609.0 59 61652.5 60 50192.5 61 41472.0 62 34763.5 63 26090.0 64 17626.5 65 12825.0 66 9731.0 67 7530.5 68 5829.5 69 4464.5 70 3389.5 71 2350.0 72 1654.5 73 1241.0 74 904.5 75 607.0 76 399.5 77 276.0 78 182.0 79 102.5 80 60.5 81 34.5 82 24.0 83 17.0 84 14.0 85 12.0 86 9.5 87 9.5 88 6.0 89 2.0 90 1.5 91 3.5 92 2.5 93 0.5 94 2.5 95 2.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4566266086612502 2 0.03941788590182492 3 0.05060794508640891 4 0.053603993190668504 5 0.0788718687687614 6 0.07861919001298047 7 0.04353293992454291 8 0.038226686053143395 9 0.043605133854766026 10-11 0.046619230441581394 12-13 0.04210710980263623 14-15 0.0365481771754558 16-17 0.03705353468701765 18-19 0.04537388514523253 20-21 0.05358594470811272 22-23 0.04540998211034409 24-25 0.06289896170689553 26-27 0.05450641731845753 28-29 0.04228759462819404 30-31 0.04479633370344755 32-33 0.043081727860648386 34-35 0.04806310904604384 36-37 0.04252222490141919 38-39 0.024816663514198378 40-41 0.02337278490973593 42-43 0.023228397049289685 44-45 0.014962192038742151 46-47 0.02593566943265678 48-49 0.026080057293103025 50-51 0.01608119795720055 52-53 0.015431452585192447 54-55 0.016153391887423672 56-57 0.01525096775963464 58-59 0.013734895224949067 60-61 0.017579222009330344 62-63 0.01608119795720055 64-65 0.017236300840770512 66-67 0.009655938167342643 68-69 0.012633937789046448 70-71 0.017236300840770512 72-73 0.01552169499797135 74-75 0.013121246818052526 76-77 0.009746180580121546 78-79 0.013987573980729997 80-81 0.01692947663732224 82-83 0.014547076939959196 84-85 0.013121246818052526 86-87 0.003772132854158154 88-89 0.02691028749066893 90-91 0.029761947734482274 92-93 0.016009004026977428 94-95 0.03068242034482709 96-97 0.025484457368762262 98-99 0.02198305175294082 100-101 0.14725756917261423 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2770316.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.8284910610492 #Duplication Level Percentage of deduplicated Percentage of total 1 72.04475835551106 35.89881597713032 2 14.773274349373333 14.722599377203533 3 5.513802839588707 8.242334265145008 4 2.612888997515971 5.207852642249534 5 1.4250983670493065 3.5505250621816096 6 0.8762314731612588 2.6196775276695456 7 0.5689760073618673 1.9845851127747585 8 0.3813664460808742 1.5202331639619953 9 0.29089432568156204 1.3045342776240283 >10 1.3682310196384435 12.210208859645881 >50 0.08966818056890384 3.0722686673692348 >100 0.04816599073894183 4.453093548425099 >500 0.0032123131880299922 1.1554853792697701 >1k 0.003212313187991539 2.8448229746403393 >5k 7.300711790889862E-5 0.2644341914126498 >10k+ 1.4601423581779723E-4 0.9485289732966612 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14686 0.530120029628389 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11388 0.41107223869045983 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7269 0.26238883939593893 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3663 0.13222318320364898 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3468 0.12518427500689452 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3330 0.12020289382149907 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3258 0.11760391233346665 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3119 0.11258643418295963 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 1.0829089533468384E-4 0.0 0.0 0.0 0.0 3 1.0829089533468384E-4 0.0 0.0 0.0 0.0 4 1.0829089533468384E-4 0.0 0.0 0.0 0.0 5 1.0829089533468384E-4 0.0 0.0 0.0 0.0 6 1.0829089533468384E-4 0.0 0.0 0.0 0.0 7 1.4438786044624512E-4 0.0 0.0 0.0 3.609696511156128E-5 8 1.4438786044624512E-4 0.0 0.0 0.0 3.609696511156128E-5 9 1.4438786044624512E-4 0.0 0.0 3.609696511156128E-5 3.609696511156128E-5 10-11 1.4438786044624512E-4 0.0 0.0 3.609696511156128E-5 7.219393022312256E-5 12-13 1.6243634300202578E-4 0.0 0.0 3.609696511156128E-5 1.4438786044624512E-4 14-15 2.5267875578092894E-4 0.0 0.0 3.609696511156128E-5 1.804848255578064E-4 16-17 2.8877572089249025E-4 0.0 0.0 3.609696511156128E-5 2.346302732251483E-4 18-19 4.3316358133873537E-4 0.0 0.0 3.609696511156128E-5 2.5267875578092894E-4 20-21 5.414544766734192E-4 0.0 0.0 3.609696511156128E-5 2.8877572089249025E-4 22-23 5.595029592291999E-4 0.0 0.0 1.4438786044624512E-4 2.8877572089249025E-4 24-25 7.941332324543481E-4 0.0 0.0 5.414544766734192E-4 2.8877572089249025E-4 26-27 7.941332324543481E-4 0.0 0.0 0.0010468119882352772 2.8877572089249025E-4 28-29 0.0011731513661257416 0.0 0.0 0.004133102505273767 2.8877572089249025E-4 30-31 0.001299490744016206 0.0 0.0 0.01458317390507076 2.8877572089249025E-4 32-33 0.0013175392265719868 0.0 0.0 0.03671061351845782 2.8877572089249025E-4 34-35 0.0014258301219066704 0.0 0.0 0.0682413125434066 3.248726860040515E-4 36-37 0.0015160725346855738 0.0 0.0 0.11991411810060658 3.609696511156128E-4 38-39 0.0016965573602433803 0.0 0.0 0.19519433884076762 3.609696511156128E-4 40-41 0.0016965573602433803 0.0 0.0 0.2739398682316385 3.609696511156128E-4 42-43 0.0017867997730222833 0.0 0.0 0.3556814457267691 3.609696511156128E-4 44-45 0.0019853330811358703 0.0 0.0 0.4555436997079033 3.609696511156128E-4 46-47 0.00225606031947258 0.0 0.0 0.570350097245224 3.609696511156128E-4 48-49 0.0024184966624746056 0.0 0.0 0.6860408704277778 3.609696511156128E-4 50-51 0.0024184966624746056 0.0 0.0 0.8117846483939016 3.609696511156128E-4 52-53 0.002436545145030386 0.0 0.0 0.9308504878143865 3.609696511156128E-4 54-55 0.002562884522920851 0.0 0.0 1.0616839378612404 3.9706661622717406E-4 56-57 0.002598981488032412 0.0 0.0 1.2106741613592096 4.151150987829547E-4 58-59 0.002598981488032412 0.0 0.0 1.371811013617219 4.3316358133873537E-4 60-61 0.002617029970588193 0.0 0.0 1.5411599254381088 4.3316358133873537E-4 62-63 0.0026350784531439736 0.0 0.0 1.7023689716263415 4.3316358133873537E-4 64-65 0.002671175418255535 0.0 0.0 1.8725120166796856 4.3316358133873537E-4 66-67 0.0027433693484786572 0.0 0.0 2.069041943229581 4.3316358133873537E-4 68-69 0.00281556327870178 0.0 0.0 2.2825735403470215 4.3316358133873537E-4 70-71 0.0028336117612575607 0.0 0.0 2.5025484457368763 4.3316358133873537E-4 72-73 0.002869708726369122 0.0 0.0 2.726764744527339 4.3316358133873537E-4 74-75 0.0028877572089249025 3.609696511156128E-5 0.0 2.9527497946082684 4.3316358133873537E-4 76-77 0.0029238541740364637 3.609696511156128E-5 0.0 3.1880478616879806 4.3316358133873537E-4 78-79 0.0029779996217038055 3.609696511156128E-5 0.0 3.4506171859094774 4.3316358133873537E-4 80-81 0.003194581412373173 3.609696511156128E-5 0.0 3.7282930900301627 4.3316358133873537E-4 82-83 0.0032487268600405153 3.609696511156128E-5 0.0 4.013224484138272 4.3316358133873537E-4 84-85 0.003320920790263638 3.609696511156128E-5 0.0 4.302469465577213 4.3316358133873537E-4 86-87 0.0033931147204867606 5.414544766734192E-5 0.0 4.601677209386944 4.692605464502966E-4 88-89 0.0033931147204867606 7.219393022312256E-5 0.0 4.9150530120029625 4.692605464502966E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6370 0.0 54.012802 1 GTATCAA 11185 0.0 41.768375 1 ATCAACG 14295 0.0 32.315132 3 TATCAAC 14405 0.0 32.266323 2 TCAACGC 14605 0.0 31.56414 4 CAACGCA 14710 0.0 31.338839 5 AACGCAG 15260 0.0 30.268332 6 ACGCAGA 17200 0.0 26.624666 7 CGCAGAG 17175 0.0 26.6081 8 TGGTATC 2795 0.0 25.505466 2 GTGGTAT 3090 0.0 24.246109 1 GCAGAGT 18750 0.0 24.068556 9 GAGTACT 12015 0.0 22.792885 12-13 GTAAGGT 1675 0.0 22.414865 4 TACATGG 10215 0.0 22.099329 2 GTACATG 10485 0.0 21.982626 1 GTACTTT 12535 0.0 21.754179 14-15 CAGAGTA 18105 0.0 21.35727 10-11 AGAGTAC 17210 0.0 21.143055 10-11 ACATGGG 10460 0.0 20.809307 3 >>END_MODULE