FastQCFastQC Report
Thu 26 May 2016
SRR938110_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938110_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2770316
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141540.5109164441890384No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116960.4221901039448207No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74140.2676228993371153No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA36330.1311402742503021No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA35170.12695302629736102No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA34710.1252925659022292No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC33550.1211053179492881No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA32760.11825365770547476No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG28950.1045007139979699No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28320.10222660519594155No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64700.053.3017121
GTATCAA115500.042.3607861
ATCAACG145850.033.250993
TATCAAC147950.032.8450132
TCAACGC149150.032.7989584
CAACGCA150400.032.431625
AACGCAG156400.031.1874436
ACGCAGA177200.027.4462077
CGCAGAG177250.027.4116698
TAAGGTG17750.026.7576355
TGGTATC27850.025.754942
GCAGAGT194000.024.8246029
AGGTAAG17500.023.8865492
GTAAGGT17550.023.8150584
GTGGTAT31100.023.6744631
GAGTACT120700.023.55065312-13
TACATGG100950.022.2568452
CAGAGTA185150.021.89403710-11
GTACTTT129600.021.87839314-15
GTACATG105950.021.7445071