##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938110_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2770316 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.52369440886888 34.0 31.0 34.0 31.0 34.0 2 32.75581161138296 34.0 31.0 34.0 31.0 34.0 3 32.71382326059554 34.0 31.0 34.0 31.0 34.0 4 36.23434763398833 37.0 35.0 37.0 35.0 37.0 5 36.20127487261381 37.0 37.0 37.0 35.0 37.0 6 36.257656527269816 37.0 37.0 37.0 35.0 37.0 7 36.2181772765273 37.0 37.0 37.0 35.0 37.0 8 36.21019587657148 37.0 37.0 37.0 35.0 37.0 9 37.92447792959359 39.0 38.0 39.0 35.0 39.0 10-11 37.8951890325869 39.0 38.0 39.0 35.0 39.0 12-13 37.841794221309044 39.0 38.0 39.0 35.0 39.0 14-15 39.125901702188486 40.0 38.5 41.0 36.0 41.0 16-17 39.06735404914096 40.0 38.0 41.0 36.0 41.0 18-19 39.07758591438666 40.0 38.5 41.0 36.0 41.0 20-21 39.08954321456469 40.0 39.0 41.0 36.0 41.0 22-23 39.109378316408666 40.0 39.0 41.0 36.0 41.0 24-25 39.0616956693749 40.0 39.0 41.0 35.0 41.0 26-27 38.89870216971638 40.0 38.0 41.0 35.0 41.0 28-29 38.824539330531245 40.0 38.0 41.0 35.0 41.0 30-31 38.5984292044662 40.0 38.0 41.0 34.5 41.0 32-33 38.387908455208716 40.0 38.0 41.0 34.0 41.0 34-35 38.182406447495524 40.0 38.0 41.0 34.0 41.0 36-37 38.09002113838277 40.0 38.0 41.0 34.0 41.0 38-39 38.024551892275106 40.0 38.0 41.0 33.5 41.0 40-41 37.894217302286094 40.0 37.5 41.0 33.0 41.0 42-43 37.7022260276445 40.0 37.0 41.0 33.0 41.0 44-45 37.4448308424021 40.0 37.0 41.0 33.0 41.0 46-47 37.08597430762411 40.0 37.0 41.0 31.5 41.0 48-49 36.699380503884754 40.0 36.0 41.0 31.0 41.0 50-51 36.39929795012554 39.5 36.0 41.0 30.0 41.0 52-53 35.94952669659345 39.0 35.0 40.5 29.0 41.0 54-55 35.64358091279118 39.0 35.0 40.0 28.0 41.0 56-57 35.377428784297535 38.0 35.0 40.0 28.0 41.0 58-59 35.012504169199474 38.0 34.5 40.0 27.0 41.0 60-61 34.61756402518702 38.0 34.0 40.0 26.0 41.0 62-63 34.20539858990816 37.0 34.0 40.0 26.0 41.0 64-65 33.78158628835122 36.5 33.0 39.5 25.0 41.0 66-67 33.29376757019777 36.0 33.0 39.0 23.5 40.5 68-69 32.78355321198015 35.0 32.0 39.0 22.0 40.0 70-71 32.21427609702286 35.0 31.5 38.0 20.5 40.0 72-73 31.76449870700671 35.0 31.0 37.0 19.5 39.5 74-75 31.23347625325053 35.0 31.0 37.0 18.0 39.0 76-77 30.005015312332603 33.5 29.5 35.0 17.5 37.5 78-79 30.696860213780667 34.0 30.5 36.0 17.5 38.0 80-81 30.53751828311283 34.0 31.0 35.5 14.0 37.0 82-83 30.097578940453005 34.0 30.0 35.0 7.5 37.0 84-85 29.61275951913067 34.0 30.0 35.0 4.5 36.5 86-87 29.109884215374706 34.0 29.0 35.0 2.0 36.0 88-89 28.518877449359564 34.0 29.0 35.0 2.0 35.5 90-91 28.028074775585168 33.0 27.5 35.0 2.0 35.0 92-93 27.44702463545675 33.0 26.5 35.0 2.0 35.0 94-95 26.747408237904992 33.0 25.0 35.0 2.0 35.0 96-97 25.982500552283568 32.0 24.5 34.0 2.0 35.0 98-99 25.01945590322548 32.0 19.0 34.0 2.0 35.0 100-101 21.53641389646524 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.004390625187774333 1101 2 0.04041892883956422 1101 3 0.1602594761463365 1101 4 0.05698994515920219 1101 5 0.005158168973594002 1101 6 0.06648684159093676 1101 7 0.06135107958412789 1101 8 0.1746540877391567 1101 9 0.10043967706301515 1101 10-11 0.20305845110200949 1101 12-13 0.1264404456626238 1101 14-15 0.247474213934602 1101 16-17 0.19870518764558653 1101 18-19 0.2430004794687619 1101 20-21 0.22638443043803846 1101 22-23 0.22386379945098156 1101 24-25 0.22955504026175078 1101 26-27 0.310458357182938 1101 28-29 0.2446400505342936 1101 30-31 0.13121005518605244 1101 32-33 0.19090595586942527 1101 34-35 0.12068179881223529 1101 36-37 0.19935145456800996 1101 38-39 0.33948031307689774 1101 40-41 0.38077360581427655 1101 42-43 0.4335753177999848 1101 44-45 0.43854036636636096 1101 46-47 0.44897564088869046 1101 48-49 0.4839389733893782 1101 50-51 0.5086796277197152 1101 52-53 0.5355667774305388 1101 54-55 0.4308261249344767 1101 56-57 0.568587943444733 1101 58-59 0.5748485644711323 1101 60-61 0.598799539307393 1101 62-63 0.697703858914771 1101 64-65 0.5761785566774833 1101 66-67 0.6495491371963595 1101 68-69 0.665480648818928 1101 70-71 0.7368662602496272 1101 72-73 0.8975759906528111 1101 74-75 0.97265164078118 1101 76-77 0.9355025942249178 1101 78-79 0.8600309618325142 1101 80-81 1.025349167676037 1101 82-83 1.0479852527240396 1101 84-85 1.1375305771121198 1101 86-87 1.2554162507690059 1101 88-89 1.5399779708083479 1101 90-91 1.5121208520446672 1101 92-93 1.891878304037835 1101 94-95 1.8779160762688818 1101 96-97 2.0399833266301535 1101 98-99 2.3446950356033405 1101 100-101 2.7097895019140203 1102 1 0.08608888394489611 1102 2 0.1418764131107011 1102 3 0.2477581453818516 1102 4 0.05434736558810016 1102 5 0.03223789115475739 1102 6 0.0594026955868614 1102 7 0.12293954897930348 1102 8 0.06759119951338022 1102 9 0.11547654053123324 1102 10-11 0.10048286626332015 1102 12-13 0.09430778058839451 1102 14-15 0.21463086033229928 1102 16-17 0.23586418032319756 1102 18-19 0.24264578120690317 1102 20-21 0.26149430421093456 1102 22-23 0.2724136193111164 1102 24-25 0.1772988044458046 1102 26-27 0.22925373022249573 1102 28-29 0.13744684213606462 1102 30-31 0.09675548602127293 1102 32-33 0.2007574890651469 1102 34-35 0.16855012944269987 1102 36-37 0.16015505715849798 1102 38-39 0.25890480610299704 1102 40-41 0.10495281267520085 1102 42-43 0.21164023636753626 1102 44-45 0.2612061365542715 1102 46-47 0.22756743883161334 1102 48-49 0.3038298627603879 1102 50-51 0.2984769794208688 1102 52-53 0.3299336901292875 1102 54-55 0.3509548464668839 1102 56-57 0.45532446792545755 1102 58-59 0.4640800354921595 1102 60-61 0.4417241961596545 1102 62-63 0.39971201266811107 1102 64-65 0.44403424633734545 1102 66-67 0.38947848006227304 1102 68-69 0.5722465999908124 1102 70-71 0.654529923609104 1102 72-73 0.565666359983453 1102 74-75 0.6408218559808674 1102 76-77 0.7132893011883361 1102 78-79 0.8296108054620888 1102 80-81 0.9060396285839474 1102 82-83 0.9799340914188477 1102 84-85 1.0000280663169931 1102 86-87 1.0012841624455255 1102 88-89 1.0374088632181504 1102 90-91 1.123915404747592 1102 92-93 1.4309188857223134 1102 94-95 1.5413527320517026 1102 96-97 1.780864962838244 1102 98-99 1.9961292293426673 1102 100-101 2.556034437687625 1103 1 0.2608261750487273 1103 2 0.2952176844391232 1103 3 0.39981384101192674 1103 4 0.19931460554563074 1103 5 0.06527426124559099 1103 6 0.06485611402435865 1103 7 0.15986328334741984 1103 8 0.21218387521070525 1103 9 0.2772716975635916 1103 10-11 0.25365286586009717 1103 12-13 0.1982834590142062 1103 14-15 0.32701100965199714 1103 16-17 0.3418670519166369 1103 18-19 0.42241023859670435 1103 20-21 0.42126790136400416 1103 22-23 0.45243562669092086 1103 24-25 0.49776422475237325 1103 26-27 0.5567236998541247 1103 28-29 0.5919892689013366 1103 30-31 0.5731572458210152 1103 32-33 0.5499311350795892 1103 34-35 0.6096337408825789 1103 36-37 0.5910979589384624 1103 38-39 0.5978699453381182 1103 40-41 0.6010716746639915 1103 42-43 0.6653266997930629 1103 44-45 0.6311882602525856 1103 46-47 0.7362761072961916 1103 48-49 0.8343009124021066 1103 50-51 0.8402196460653926 1103 52-53 0.8385805900812997 1103 54-55 0.959611985167534 1103 56-57 0.9301623478166334 1103 58-59 0.9041796992342626 1103 60-61 0.9805523773072267 1103 62-63 1.0955631649819466 1103 64-65 1.1230862409957965 1103 66-67 1.1176820026661858 1103 68-69 1.1813256423397007 1103 70-71 1.1562174393724263 1103 72-73 1.0495230356870948 1103 74-75 1.1581287013947446 1103 76-77 0.9932302287478088 1103 78-79 1.3987328347425318 1103 80-81 1.4147102476447806 1103 82-83 1.5144176236369056 1103 84-85 1.4140426401280912 1103 86-87 1.5154827174774148 1103 88-89 1.6310433066848375 1103 90-91 1.5182063536147261 1103 92-93 1.572190787354426 1103 94-95 1.666418354919454 1103 96-97 1.9434494720463995 1103 98-99 2.4335345011517973 1103 100-101 2.7383806212434827 1104 1 0.06827277842944568 1104 2 0.10433745144092654 1104 3 0.1832436573560443 1104 4 0.044489231880817215 1104 5 -0.020838084982358396 1104 6 0.043972326767601544 1104 7 0.0685882686339454 1104 8 0.021955136368127626 1104 9 -0.027519644435223256 1104 10-11 0.0109910787443539 1104 12-13 0.05987134920920312 1104 14-15 0.10068136440153808 1104 16-17 0.15079435653674267 1104 18-19 0.164201088467415 1104 20-21 0.17308873939198577 1104 22-23 0.16582190696507837 1104 24-25 0.06989966502715816 1104 26-27 0.1504874150472162 1104 28-29 0.13256159806309853 1104 30-31 0.1493757665299711 1104 32-33 0.14339032801827045 1104 34-35 0.08866955111682984 1104 36-37 0.049243506546098104 1104 38-39 0.06750459777624229 1104 40-41 0.14267610583152646 1104 42-43 0.1771402926016563 1104 44-45 0.08546207630315195 1104 46-47 0.07272301429981098 1104 48-49 0.061727233485477484 1104 50-51 0.1271357295890354 1104 52-53 0.1474933251192354 1104 54-55 0.22938470688060164 1104 56-57 0.26010240747637425 1104 58-59 0.13786302630807512 1104 60-61 0.29375090782995983 1104 62-63 0.19491674319937857 1104 64-65 0.20415355462386486 1104 66-67 0.18861500273381893 1104 68-69 0.4362249791179238 1104 70-71 0.36900366870700907 1104 72-73 0.30180794188776616 1104 74-75 0.4513428015845413 1104 76-77 0.42444615211396197 1104 78-79 0.47193509220036134 1104 80-81 0.4957838567713111 1104 82-83 0.5415093119960623 1104 84-85 0.7198259728138865 1104 86-87 0.7067117210906666 1104 88-89 0.8155551933054284 1104 90-91 0.7325412755721317 1104 92-93 0.9484707521284115 1104 94-95 1.1126628922254973 1104 96-97 1.4578796538891758 1104 98-99 1.7752969673179884 1104 100-101 2.1690873287193675 1105 1 0.13434094704616228 1105 2 0.10547188448644107 1105 3 0.21074410501489638 1105 4 0.08556684500748446 1105 5 0.06865246832398242 1105 6 0.030831082756400008 1105 7 0.08606033908889543 1105 8 0.02369090627584569 1105 9 0.015951851706176967 1105 10-11 0.04217160973326628 1105 12-13 0.03852320819447641 1105 14-15 0.07812017707923502 1105 16-17 0.12940022130446494 1105 18-19 0.15659034203449806 1105 20-21 0.18081836108749627 1105 22-23 0.16147565812789821 1105 24-25 0.20840040093464296 1105 26-27 0.12716111323343426 1105 28-29 0.17020247412055056 1105 30-31 0.10796186333783453 1105 32-33 0.10462470909168786 1105 34-35 0.22025259292598776 1105 36-37 0.2753771479997411 1105 38-39 0.28486289737138293 1105 40-41 0.28180997875755764 1105 42-43 0.2558875793522688 1105 44-45 0.3276027973177875 1105 46-47 0.36329373566383794 1105 48-49 0.51454590107803 1105 50-51 0.4749116566796019 1105 52-53 0.4500487474689905 1105 54-55 0.48455557608123456 1105 56-57 0.4283654965845116 1105 58-59 0.5483049147296555 1105 60-61 0.5200376813669934 1105 62-63 0.5796418588263847 1105 64-65 0.6302546316255544 1105 66-67 0.8569093460728752 1105 68-69 0.7981040708290337 1105 70-71 0.9408974075974257 1105 72-73 0.8743950060754955 1105 74-75 0.7495230410492724 1105 76-77 0.7064956599075956 1105 78-79 0.9434512963590045 1105 80-81 1.0972517410700569 1105 82-83 0.999273791030344 1105 84-85 1.2096854079339252 1105 86-87 1.2230913947582813 1105 88-89 1.2817252862000998 1105 90-91 1.2138582060299647 1105 92-93 1.2619556016058517 1105 94-95 1.3133957152277382 1105 96-97 1.3930309462038544 1105 98-99 1.6268486511703877 1105 100-101 2.124242011230976 1106 1 0.00490581862527506 1106 2 -0.016241146136756868 1106 3 0.07887465798706472 1106 4 7.789044866299832E-4 1106 5 0.002505142291632012 1106 6 0.007763116380552049 1106 7 0.010468347178601789 1106 8 -0.02829442955441408 1106 9 -0.04804321981684723 1106 10-11 0.03373156654318876 1106 12-13 -0.04145553946202085 1106 14-15 -0.06777246879799037 1106 16-17 -0.04519818005113052 1106 18-19 -0.06771309145004523 1106 20-21 -0.025895410283467868 1106 22-23 0.01815302477748304 1106 24-25 0.022059104430965704 1106 26-27 -0.0215562595952008 1106 28-29 -0.1596799895063583 1106 30-31 -0.12513274359661608 1106 32-33 -0.11980314892937116 1106 34-35 -0.27903526113035326 1106 36-37 -0.07494257297295803 1106 38-39 -0.12499033967001338 1106 40-41 -0.14380451237772007 1106 42-43 -0.14941071792958383 1106 44-45 -0.18435204132045868 1106 46-47 -0.6786843877610877 1106 48-49 -0.35406402905442036 1106 50-51 -0.3748575120801405 1106 52-53 -0.4747370156224733 1106 54-55 -0.38735048392091187 1106 56-57 -0.21383692052027925 1106 58-59 -0.15990326913607333 1106 60-61 -0.2676294086594311 1106 62-63 -0.2763812904640801 1106 64-65 -0.1920177031059751 1106 66-67 -0.32810058152891486 1106 68-69 -0.5391526554770536 1106 70-71 -0.17533633022109996 1106 72-73 -0.2804736047772778 1106 74-75 -0.1734628499463966 1106 76-77 -0.007006102597596708 1106 78-79 0.08251489364106845 1106 80-81 0.07872672013931847 1106 82-83 0.06653289774652649 1106 84-85 0.1427916664881117 1106 86-87 0.2968444341966432 1106 88-89 0.44028274186594984 1106 90-91 0.4189191345111638 1106 92-93 0.2992577876816931 1106 94-95 0.6879111317441478 1106 96-97 0.8357880805802438 1106 98-99 1.1122270546559605 1106 100-101 1.6006584211402277 1107 1 0.13704654329465882 1107 2 0.09497177943949708 1107 3 0.1735049630954535 1107 4 0.09885579345696982 1107 5 0.06031079752683155 1107 6 -8.503873704910347E-4 1107 7 0.0054433106731863745 1107 8 0.06121625012610821 1107 9 0.09214938687650687 1107 10-11 0.07605715343789399 1107 12-13 0.08532021976188275 1107 14-15 0.15631755620895404 1107 16-17 0.11286877708235465 1107 18-19 0.1347515411606679 1107 20-21 0.1112526154683593 1107 22-23 0.1526821197388486 1107 24-25 0.20796771970825745 1107 26-27 0.1903968242992633 1107 28-29 0.11589301245044936 1107 30-31 0.13031646510792427 1107 32-33 0.14701446589271683 1107 34-35 0.1594789833701853 1107 36-37 0.1883929871412633 1107 38-39 0.1794884458082251 1107 40-41 0.23415213042493832 1107 42-43 0.24371857921560292 1107 44-45 0.21632959010198505 1107 46-47 0.32469421031284185 1107 48-49 0.20292579672460676 1107 50-51 0.08981943719064134 1107 52-53 0.0035282235396962847 1107 54-55 0.20043985973246237 1107 56-57 0.03416045463712436 1107 58-59 0.11058557823879056 1107 60-61 0.23499247803486156 1107 62-63 0.28041640156621384 1107 64-65 0.19608655753379622 1107 66-67 0.3620822706818032 1107 68-69 0.45325362759211885 1107 70-71 0.3781477936194477 1107 72-73 0.3336420183568123 1107 74-75 0.4614771156468791 1107 76-77 0.5375009413212197 1107 78-79 0.6771984050183413 1107 80-81 0.7692701926169008 1107 82-83 0.9230828850361412 1107 84-85 0.9867051649185257 1107 86-87 0.9936751141136426 1107 88-89 1.1539295586531217 1107 90-91 1.306124800387618 1107 92-93 1.4911668877272142 1107 94-95 1.6572111145999067 1107 96-97 1.689435370312026 1107 98-99 1.6669792612381684 1107 100-101 2.144928992752913 1108 1 0.09140592069791609 1108 2 0.06805472065524043 1108 3 0.12224734174783691 1108 4 0.06786955772604131 1108 5 0.01827030074560554 1108 6 0.030746623795344874 1108 7 0.09567722734046669 1108 8 0.006320021529504061 1108 9 0.07040022035737081 1108 10-11 0.01685088697109194 1108 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-1.6457228598601965 2316 82-83 -1.6193952438986017 2316 84-85 -1.494892048615899 2316 86-87 -1.5664306533122598 2316 88-89 -1.7169522808240636 2316 90-91 -1.8754745962410269 2316 92-93 -2.2546674644664897 2316 94-95 -2.3227949821143596 2316 96-97 -2.45291652792827 2316 98-99 -2.688611049930511 2316 100-101 -3.536673743312093 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 10.0 4 19.0 5 41.0 6 94.0 7 251.0 8 589.0 9 1162.0 10 2003.0 11 3363.0 12 5479.0 13 7805.0 14 10742.0 15 14313.0 16 18944.0 17 27052.0 18 26997.0 19 19578.0 20 16847.0 21 16661.0 22 17314.0 23 18807.0 24 21608.0 25 24690.0 26 28883.0 27 34968.0 28 42292.0 29 51691.0 30 63261.0 31 78384.0 32 98827.0 33 130068.0 34 182215.0 35 276338.0 36 432750.0 37 620842.0 38 439041.0 39 36378.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.14197369541958 19.04266516888326 14.986774071983126 22.828587063714032 2 15.57662718924254 20.171175535789967 40.15444642677928 24.09775084818821 3 19.317204677594145 24.138473767770748 30.159897940249998 26.38442361438511 4 12.786375272712572 17.03419393311088 36.947193027798996 33.23223776637755 5 13.417940112030946 37.58285185824237 34.4282183989783 14.570989630748372 6 32.06553343649865 36.48066949883966 17.07093535707724 14.382861707584455 7 28.307961979788587 33.361573192372276 20.336777465097843 17.99368736274129 8 26.53584631050715 34.70568127139425 19.61424575490206 19.14422666319654 9 26.3238200985014 16.6268035848618 20.249170130772086 36.80020618586472 10-11 24.90315184260568 26.77145856284987 27.02854114837441 21.29684844617004 12-13 25.938412802005256 24.07135142705742 27.589054822626736 22.40118094831059 14-15 22.60393760134223 25.467563989090053 26.194990030018232 25.73350837954948 16-17 21.720602480332108 28.60489305456113 27.268348052179636 22.406156412927125 18-19 21.730282754747112 26.78210716755778 29.950554377190187 21.537055700504926 20-21 22.318194025519112 25.966911355960836 30.320638511996467 21.39425610652359 22-23 21.992184285114043 25.95709298145049 30.32349017224028 21.727232561195184 24-25 21.818879486663015 26.169905705805867 30.346887266421348 21.66432754110977 26-27 21.440005256956304 26.496233206669327 30.052585810514525 22.01117572585984 28-29 21.595454656206936 26.919412375701974 29.980083438390864 21.505049529700223 30-31 21.6916864072194 26.395054709531866 29.809308516638467 22.103950366610263 32-33 21.35890542495463 26.407714945541084 29.88223806339417 22.35114156611012 34-35 22.195792678127745 26.63056467562363 29.552450359912747 21.62119228633588 36-37 22.303465407207774 26.163229670206096 29.594612571448785 21.93869235113734 38-39 21.768372466501642 26.474480158053904 29.618769334307693 22.138378041136765 40-41 22.157897672816514 26.292015682208003 29.71699705611081 21.83308958886467 42-43 21.972693059212094 26.33321443064628 29.408738336585106 22.285354173556524 44-45 21.945642387472176 26.608522808089162 29.59910845602171 21.846726348416944 46-47 22.38572089159959 26.888281484353254 28.799385760960085 21.92661186308707 48-49 22.06867325534679 27.004227329317654 28.61287575709118 22.314223658244376 50-51 22.28893743529619 27.176556753814367 28.585673980874382 21.94883183001506 52-53 22.584734015902878 26.888557117671773 28.562860698923874 21.963848167501467 54-55 22.125454280305927 27.129847280960007 28.579501399840307 22.16519703889376 56-57 22.194850695732907 27.330943473596513 28.29585144799366 22.178354382676922 58-59 22.070334214580576 26.957845242203415 28.408058864042946 22.563761679173062 60-61 21.813377968433926 27.156468792729783 28.64555884599446 22.384594392841827 62-63 21.891654237278345 27.082903177832417 28.861833812460386 22.16360877242885 64-65 21.711674769232104 27.263442148837896 28.521674061731584 22.503209020198415 66-67 21.610629576205966 27.345515372297953 28.469523966288225 22.574331085207856 68-69 22.198951670047155 26.93277036404938 28.57458963993573 22.293688325967732 70-71 21.983809789208163 27.083625117134652 28.12641229375999 22.806152799897195 72-73 21.899021970602263 27.221646776332875 28.15179354120496 22.727537711859895 74-75 21.984297098237167 27.653217178112534 27.784068676641944 22.578417047008355 76-77 22.048748229443863 27.304881464785968 28.161606834743765 22.484763471026408 78-79 22.020791129964955 27.520217910158983 28.028354888034436 22.430636071841622 80-81 22.186801014976112 27.49755758513077 27.821492276229588 22.494149123663533 82-83 22.230368176056565 27.629041925732363 27.817993160647536 22.322596737563536 84-85 22.02532920439181 27.83015964723144 27.692444033403348 22.452067114973406 86-87 22.31757503213104 27.71129371892142 27.536258303037236 22.434872945910303 88-89 22.110846981452827 27.826696210845377 27.300763144401113 22.761693663300683 90-91 21.98112664367556 27.86170969873491 27.46145000643455 22.69571365115498 92-93 22.102692081320686 27.52661588139732 27.659706568785268 22.710985468496727 94-95 21.911348581440855 27.328664543439345 27.797203996230024 22.96278287888977 96-97 22.168210960012736 26.976035507977997 27.764052864899092 23.091700667110178 98-99 21.767517630704734 27.409595824870074 27.426019985496204 23.396866558928984 100-101 22.0807860135902 26.967398398401286 27.69690408601157 23.254911501996943 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1187.0 1 789.0 2 567.0 3 790.5 4 696.0 5 651.5 6 1280.0 7 2895.5 8 4713.0 9 5458.5 10 4497.5 11 3285.0 12 3038.5 13 3222.5 14 3439.5 15 3495.0 16 3751.0 17 4081.0 18 4408.5 19 4910.0 20 5570.0 21 6533.5 22 7907.5 23 9816.0 24 12125.5 25 14946.5 26 18889.0 27 23642.0 28 27653.5 29 31414.5 30 35692.5 31 41624.0 32 50714.0 33 54509.5 34 54184.0 35 57580.0 36 63349.5 37 68363.5 38 71300.0 39 74166.0 40 78043.0 41 82338.0 42 84543.5 43 87030.0 44 90927.5 45 96376.5 46 99345.5 47 101122.0 48 102617.0 49 100286.0 50 96883.5 51 94083.0 52 93241.0 53 94450.5 54 96573.0 55 91852.0 56 83107.0 57 74753.0 58 68634.0 59 61030.0 60 48797.0 61 40172.5 62 33613.5 63 24312.5 64 15631.5 65 11132.0 66 8394.5 67 6559.5 68 4982.0 69 3847.0 70 2967.5 71 1879.0 72 1278.5 73 1019.0 74 730.0 75 458.5 76 321.0 77 202.5 78 110.5 79 54.0 80 30.0 81 15.0 82 10.0 83 6.0 84 2.5 85 2.5 86 2.0 87 3.0 88 2.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.010612507742799015 3 0.0062808719294116635 4 0.0 5 3.609696511156128E-4 6 1.804848255578064E-4 7 0.0 8 1.0829089533468384E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 5.414544766734192E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.001804848255578064 26-27 0.023553269735293734 28-29 3.068242034482709E-4 30-31 1.263393778904645E-4 32-33 0.0039165207146044 34-35 0.01725434932332629 36-37 0.008573029213995803 38-39 0.022668894090060482 40-41 0.024563984758417454 42-43 0.019636749020689338 44-45 0.010468119882352772 46-47 0.0021297209415821155 48-49 3.248726860040515E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.8877572089249025E-4 68-69 1.804848255578064E-4 70-71 0.0 72-73 1.804848255578064E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.6243634300202575E-4 82-83 8.843756452332513E-4 84-85 0.004133102505273766 86-87 7.03890819675445E-4 88-89 5.414544766734192E-5 90-91 0.004133102505273766 92-93 8.843756452332513E-4 94-95 1.804848255578064E-4 96-97 3.248726860040515E-4 98-99 2.52678755780929E-4 100-101 0.0030321450693711477 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2770316.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.87320480496858 #Duplication Level Percentage of deduplicated Percentage of total 1 70.25980660304076 33.635621110648536 2 15.57753016855676 14.91492584229789 3 5.819315811574437 8.357678930168849 4 2.8567876437604505 5.470543198161907 5 1.5419682181213876 3.690948015443883 6 0.92622314770407 2.660476226708377 7 0.6142420127269222 2.0584013572564444 8 0.44875335413502876 1.7186608983538263 9 0.2994414306678751 1.2901698843710385 >10 1.4948078856429299 12.791304899174635 >50 0.10092062210256192 3.3132942637429963 >100 0.05294873147821497 4.725037868157636 >500 0.003400486166569615 1.1171884033766548 >1k 0.00362718524430402 3.0523893764190584 >5k 7.556635925633376E-5 0.26820914521630435 >10k+ 1.5113271851266752E-4 0.9351505805019514 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14154 0.5109164441890384 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11696 0.4221901039448207 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7414 0.2676228993371153 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3633 0.1311402742503021 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3517 0.12695302629736102 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3471 0.1252925659022292 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3355 0.1211053179492881 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3276 0.11825365770547476 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2895 0.1045007139979699 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2832 0.10222660519594155 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 3.609696511156128E-5 0.0 0.0 0.0 0.0 6 1.0829089533468384E-4 0.0 0.0 0.0 3.609696511156128E-5 7 2.1658179066936768E-4 0.0 0.0 0.0 3.609696511156128E-5 8 2.1658179066936768E-4 0.0 0.0 0.0 3.609696511156128E-5 9 2.1658179066936768E-4 0.0 0.0 3.609696511156128E-5 3.609696511156128E-5 10-11 2.1658179066936768E-4 0.0 0.0 3.609696511156128E-5 3.609696511156128E-5 12-13 2.5267875578092894E-4 0.0 0.0 3.609696511156128E-5 3.609696511156128E-5 14-15 3.609696511156128E-4 0.0 0.0 3.609696511156128E-5 5.414544766734192E-5 16-17 3.609696511156128E-4 0.0 0.0 3.609696511156128E-5 7.219393022312256E-5 18-19 4.151150987829547E-4 0.0 0.0 3.609696511156128E-5 7.219393022312256E-5 20-21 4.692605464502966E-4 0.0 0.0 3.609696511156128E-5 7.219393022312256E-5 22-23 5.053575115618579E-4 0.0 0.0 1.4438786044624512E-4 9.02424127789032E-5 24-25 7.219393022312256E-4 0.0 0.0 5.414544766734192E-4 1.0829089533468384E-4 26-27 7.580362673427869E-4 0.0 0.0 0.0010648604707910578 1.0829089533468384E-4 28-29 9.56569575456374E-4 0.0 0.0 0.004277490365720012 1.0829089533468384E-4 30-31 0.0010829089533468384 0.0 0.0 0.014781707213184343 1.0829089533468384E-4 32-33 0.0010829089533468384 0.0 0.0 0.0372881649602428 1.0829089533468384E-4 34-35 0.0012994907440162062 0.0 0.0 0.06914373667119564 1.0829089533468384E-4 36-37 0.0015882664649086962 0.0 0.0 0.1211775118795112 1.0829089533468384E-4 38-39 0.001913139150912748 0.0 0.0 0.19721576888701506 1.0829089533468384E-4 40-41 0.0019672845985800897 0.0 0.0 0.27662909213244985 1.0829089533468384E-4 42-43 0.002093623976470554 0.0 0.0 0.3589301725868096 1.0829089533468384E-4 44-45 0.002310205767139922 0.0 0.0 0.4592977840795057 1.0829089533468384E-4 46-47 0.0025267875578092896 0.0 0.0 0.5752231875352848 1.2633937789046447E-4 48-49 0.002671175418255535 0.0 0.0 0.6918885787758509 1.804848255578064E-4 50-51 0.002671175418255535 0.0 0.0 0.8177586961198651 1.804848255578064E-4 52-53 0.002671175418255535 0.0 0.0 0.9377450081506947 1.804848255578064E-4 54-55 0.002707272383367096 0.0 0.0 1.06976965804623 1.804848255578064E-4 56-57 0.0027433693484786572 0.0 0.0 1.2193193845034285 1.804848255578064E-4 58-59 0.0027433693484786572 0.0 0.0 1.381466951784562 1.804848255578064E-4 60-61 0.0027433693484786572 0.0 0.0 1.5512129302216788 1.804848255578064E-4 62-63 0.0027433693484786572 3.609696511156128E-5 0.0 1.713685370188816 1.804848255578064E-4 64-65 0.0027794663135902184 3.609696511156128E-5 0.0 1.88532643929429 1.804848255578064E-4 66-67 0.00281556327870178 3.609696511156128E-5 0.0 2.0837695049950984 1.9853330811358703E-4 68-69 0.002869708726369122 3.609696511156128E-5 0.0 2.29836596258333 2.1658179066936768E-4 70-71 0.002905805691480683 3.609696511156128E-5 0.0 2.520831558565882 2.1658179066936768E-4 72-73 0.0029238541740364637 3.609696511156128E-5 0.0 2.7482785357338297 2.5267875578092894E-4 74-75 0.0029419026565922443 3.609696511156128E-5 0.0 2.97798157322125 2.8877572089249025E-4 76-77 0.002959951139148025 3.609696511156128E-5 0.0 3.218224924521246 2.8877572089249025E-4 78-79 0.002959951139148025 3.609696511156128E-5 0.0 3.486299035922256 2.8877572089249025E-4 80-81 0.0030501935519269283 3.609696511156128E-5 0.0 3.7709236058269164 2.8877572089249025E-4 82-83 0.00310433899959427 3.609696511156128E-5 0.0 4.063381217160786 2.8877572089249025E-4 84-85 0.003158484447261612 3.609696511156128E-5 0.0 4.361108985400943 2.8877572089249025E-4 86-87 0.003212629894928954 3.609696511156128E-5 0.0 4.670766800610473 2.8877572089249025E-4 88-89 0.003212629894928954 3.609696511156128E-5 0.0 4.996938977358539 2.8877572089249025E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6470 0.0 53.301712 1 GTATCAA 11550 0.0 42.360786 1 ATCAACG 14585 0.0 33.25099 3 TATCAAC 14795 0.0 32.845013 2 TCAACGC 14915 0.0 32.798958 4 CAACGCA 15040 0.0 32.43162 5 AACGCAG 15640 0.0 31.187443 6 ACGCAGA 17720 0.0 27.446207 7 CGCAGAG 17725 0.0 27.411669 8 TAAGGTG 1775 0.0 26.757635 5 TGGTATC 2785 0.0 25.75494 2 GCAGAGT 19400 0.0 24.824602 9 AGGTAAG 1750 0.0 23.886549 2 GTAAGGT 1755 0.0 23.815058 4 GTGGTAT 3110 0.0 23.674463 1 GAGTACT 12070 0.0 23.550653 12-13 TACATGG 10095 0.0 22.256845 2 CAGAGTA 18515 0.0 21.894037 10-11 GTACTTT 12960 0.0 21.878393 14-15 GTACATG 10595 0.0 21.744507 1 >>END_MODULE