FastQCFastQC Report
Thu 26 May 2016
SRR938109_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938109_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2802846
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151970.5421988935531956No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117530.4193237873218864No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75750.27026101327008334No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA36820.1313664753611151No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA36670.130831305037808No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA35490.12662129849445883No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA34050.12148366339071073No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC30900.11024508660126171No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28060.1001125284799807No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67900.050.4945641
GTATCAA119550.041.141261
TATCAAC149250.032.6692122
ATCAACG149550.032.444793
TCAACGC151450.032.1005174
CAACGCA153750.031.5894015
AACGCAG159050.030.5367556
ACGCAGA179350.026.906467
CGCAGAG178650.026.7723738
GCAGAGT193100.024.299419
GTACATG106850.023.9988121
TAAGGTG16900.023.90225
TACATGG104150.023.7730062
TGGTATC31650.023.4237562
ACATGGG103400.023.3939153
GAGTACT119500.023.2127212-13
GTGGTAT33350.023.0526031
GTACTTT125450.021.69970714-15
CAGAGTA181850.021.69868510-11
AGAGTAC171900.021.22614510-11
Produced by FastQC (version 0.11.5)