##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938109_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2802846 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.87425102913253 33.0 31.0 34.0 30.0 34.0 2 32.07160257823655 34.0 31.0 34.0 30.0 34.0 3 32.13763581730855 34.0 31.0 34.0 30.0 34.0 4 35.665062582817605 37.0 35.0 37.0 35.0 37.0 5 35.689179498267116 37.0 35.0 37.0 35.0 37.0 6 35.71688776336624 37.0 35.0 37.0 35.0 37.0 7 35.65913574987709 37.0 35.0 37.0 33.0 37.0 8 35.64318196575909 37.0 35.0 37.0 33.0 37.0 9 37.304029903890545 39.0 37.0 39.0 34.0 39.0 10-11 37.22271434106619 39.0 37.0 39.0 34.0 39.0 12-13 37.13075727314308 39.0 37.0 39.0 33.5 39.0 14-15 38.378050560037906 40.0 38.0 41.0 33.5 41.0 16-17 38.3006854461501 40.0 38.0 41.0 33.0 41.0 18-19 38.260537682056025 40.0 38.0 41.0 33.5 41.0 20-21 38.15992245025235 40.0 38.0 41.0 33.5 41.0 22-23 38.0116736702623 40.0 38.0 41.0 33.0 41.0 24-25 37.96077272886202 40.0 38.0 41.0 33.0 41.0 26-27 37.96795649850188 40.0 38.0 41.0 33.0 41.0 28-29 37.88039514122431 40.0 38.0 41.0 33.0 41.0 30-31 37.67663474910859 40.0 37.5 41.0 33.0 41.0 32-33 37.438526590472684 40.0 37.0 41.0 32.0 41.0 34-35 37.22677200245751 40.0 37.0 41.0 31.0 41.0 36-37 36.99421712787645 40.0 36.0 41.0 31.0 41.0 38-39 36.74301138913804 39.5 36.0 41.0 30.0 41.0 40-41 36.50660079076767 39.0 35.5 41.0 30.0 41.0 42-43 36.210964498227874 39.0 35.0 41.0 30.0 41.0 44-45 35.964865889884784 39.0 35.0 41.0 29.0 41.0 46-47 35.78502975190217 39.0 35.0 41.0 27.5 41.0 48-49 35.504497214616855 39.0 35.0 41.0 26.5 41.0 50-51 34.846953418061496 38.5 34.5 40.0 25.0 40.5 52-53 34.9141394140099 38.5 34.5 40.0 26.0 41.0 54-55 35.04624103500514 39.0 35.0 40.0 25.5 41.0 56-57 34.832815110070264 38.5 35.0 40.0 25.0 41.0 58-59 34.57145380088667 38.0 34.0 40.0 24.0 41.0 60-61 34.20468177702236 38.0 34.0 40.0 23.0 41.0 62-63 33.790647256395815 37.0 34.0 40.0 22.0 41.0 64-65 33.36802289529999 36.5 33.0 40.0 20.5 41.0 66-67 32.9410458155746 36.0 33.0 39.0 19.5 41.0 68-69 32.45897134555376 35.5 32.0 39.0 17.5 40.5 70-71 31.974058867308443 35.0 32.0 38.5 13.0 40.0 72-73 31.495469961603312 35.0 31.0 37.5 9.0 40.0 74-75 30.9776789377654 35.0 31.0 37.0 7.0 39.0 76-77 30.44540674014912 35.0 30.0 36.5 2.0 39.0 78-79 29.87898621615315 34.0 29.5 36.0 2.0 38.5 80-81 29.35939131154548 34.0 29.0 35.0 2.0 37.0 82-83 28.819231416924083 34.0 29.0 35.0 2.0 37.0 84-85 28.327077549034087 34.0 27.5 35.0 2.0 36.0 86-87 27.829541116422376 33.5 26.5 35.0 2.0 36.0 88-89 27.261033963335837 33.0 26.0 35.0 2.0 35.5 90-91 26.647110829492597 33.0 25.0 35.0 2.0 35.0 92-93 26.03698187485149 32.0 23.5 35.0 2.0 35.0 94-95 25.416910882724203 32.0 21.0 34.5 2.0 35.0 96-97 24.564938815760836 32.0 12.5 34.0 2.0 35.0 98-99 23.471834164274455 31.0 2.0 34.0 2.0 35.0 100-101 20.549382484802948 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.41109782051509924 1101 2 0.3305349827950721 1101 3 0.30970894243755254 1101 4 0.19437755642582744 1101 5 0.25674097918121674 1101 6 0.272385259096815 1101 7 0.16609385273004307 1101 8 0.20852828179651794 1101 9 0.33162889823535835 1101 10-11 0.36262072690163194 1101 12-13 0.20422209121626622 1101 14-15 0.2576420900179386 1101 16-17 0.34945740894485056 1101 18-19 0.30657263584254935 1101 20-21 0.3725040803863706 1101 22-23 0.3347504503823302 1101 24-25 0.44262988144347304 1101 26-27 0.42382200006154847 1101 28-29 0.4645984965040455 1101 30-31 0.45461695829808235 1101 32-33 0.5541663415874254 1101 34-35 0.6467657123573431 1101 36-37 0.5848883974106442 1101 38-39 0.6423541860661359 1101 40-41 0.5900070720372952 1101 42-43 0.6401011053220387 1101 44-45 0.6121444182165376 1101 46-47 0.7002001548939774 1101 48-49 0.7479871784103267 1101 50-51 0.6496486715260588 1101 52-53 0.6634641784139177 1101 54-55 0.6531033051920829 1101 56-57 0.6840771504534118 1101 58-59 0.7387811127303578 1101 60-61 0.604442583451096 1101 62-63 0.7255619215514741 1101 64-65 0.7136814268444311 1101 66-67 0.6184908510111455 1101 68-69 0.6902446180609658 1101 70-71 0.7483451218996393 1101 72-73 0.8356169275252832 1101 74-75 0.7434553089797511 1101 76-77 0.7760801301151616 1101 78-79 0.7760608294764069 1101 80-81 0.8999156868052367 1101 82-83 0.8373775491104567 1101 84-85 0.6373663344506362 1101 86-87 0.6044112717660504 1101 88-89 0.46536993193373277 1101 90-91 0.7588191287489714 1101 92-93 1.039582357313929 1101 94-95 1.2102715335022296 1101 96-97 1.2000219002636676 1101 98-99 1.653735777570219 1101 100-101 1.5021977456690472 1102 1 0.39954470376342144 1102 2 0.2368951672268622 1102 3 0.25394015692219796 1102 4 0.18898120669341267 1102 5 0.1423826866967488 1102 6 0.21095452355268662 1102 7 0.19484450598108083 1102 8 0.25479697800666656 1102 9 0.3203668503016175 1102 10-11 0.2618087110516072 1102 12-13 0.19169339778449768 1102 14-15 0.23884880180027324 1102 16-17 0.2582680289875867 1102 18-19 0.22243142115944892 1102 20-21 0.3249492238369953 1102 22-23 0.36983151966332173 1102 24-25 0.25217726943159136 1102 26-27 0.3167665580669379 1102 28-29 0.20309582065548426 1102 30-31 0.22081559953591778 1102 32-33 0.36499922839472987 1102 34-35 0.2957907971972844 1102 36-37 0.34839746354279555 1102 38-39 0.3518101189086238 1102 40-41 0.4497595312380511 1102 42-43 0.40750685525423336 1102 44-45 0.21964837079877952 1102 46-47 0.38697041559902345 1102 48-49 0.29949924784347814 1102 50-51 0.4340715283664949 1102 52-53 0.49147723681952016 1102 54-55 0.5485355093107032 1102 56-57 0.47440572745765763 1102 58-59 0.46507405365000665 1102 60-61 0.5223522462743304 1102 62-63 0.5813960321708507 1102 64-65 0.5496322295990055 1102 66-67 0.5937715008884297 1102 68-69 0.5750786096067415 1102 70-71 0.6912028553996876 1102 72-73 0.7468305191160418 1102 74-75 0.6694406430804491 1102 76-77 0.6111340770744853 1102 78-79 0.6413688993593816 1102 80-81 0.6821143478886782 1102 82-83 0.8829281653163719 1102 84-85 0.7728757379580173 1102 86-87 0.7067449490898063 1102 88-89 0.7945494155511881 1102 90-91 0.9909112851058488 1102 92-93 0.9691489955352317 1102 94-95 1.0743847328116054 1102 96-97 1.0105549531125035 1102 98-99 1.1476474778751822 1102 100-101 1.1447463839300411 1103 1 0.3854127501252016 1103 2 0.3295452757483588 1103 3 0.2509715189588064 1103 4 0.1740764923700553 1103 5 0.2654735748207102 1103 6 0.18841561655003147 1103 7 0.28641036587141855 1103 8 0.24097522384312953 1103 9 0.3896295934442122 1103 10-11 0.3207623950166436 1103 12-13 0.2838944740446081 1103 14-15 0.3850762865689248 1103 16-17 0.3996297628061569 1103 18-19 0.3091579437621377 1103 20-21 0.34612428392807715 1103 22-23 0.354518520984648 1103 24-25 0.4694997035858748 1103 26-27 0.4611122304460409 1103 28-29 0.47747579214161107 1103 30-31 0.4043649450891209 1103 32-33 0.5260987325265347 1103 34-35 0.412394876368694 1103 36-37 0.3886198343687184 1103 38-39 0.42509031568396694 1103 40-41 0.5520177705290834 1103 42-43 0.5512847949499076 1103 44-45 0.4824628624936125 1103 46-47 0.6807294791896012 1103 48-49 0.7207306463438172 1103 50-51 0.5777264190493696 1103 52-53 0.7066347170076668 1103 54-55 0.7102736120495479 1103 56-57 0.5012932252269877 1103 58-59 0.7071003487730749 1103 60-61 0.6821051367987181 1103 62-63 0.7421383076241241 1103 64-65 0.7092026403701013 1103 66-67 0.7578753980632627 1103 68-69 0.8747559441718522 1103 70-71 1.0537836828173184 1103 72-73 0.9139307563880372 1103 74-75 0.8353498496591243 1103 76-77 0.7815964192447993 1103 78-79 0.842134154698968 1103 80-81 0.8600256649834037 1103 82-83 0.7570954101845508 1103 84-85 0.897576248901327 1103 86-87 0.8303835985914567 1103 88-89 0.7596832200784895 1103 90-91 1.0361654586255717 1103 92-93 1.070315416425995 1103 94-95 1.2672081729398847 1103 96-97 1.2957946442143857 1103 98-99 1.4996765303269157 1103 100-101 1.5858516475287274 1104 1 0.4124248685632246 1104 2 0.28482992768294935 1104 3 0.2543353944033271 1104 4 0.24249289789851503 1104 5 0.21262254141759485 1104 6 0.2658181557975041 1104 7 0.26844712654784075 1104 8 0.2325560557116546 1104 9 0.17363601785045546 1104 10-11 0.3396455992028038 1104 12-13 0.16308476051570864 1104 14-15 0.20303496669470178 1104 16-17 0.27230761891787836 1104 18-19 0.34345349353367993 1104 20-21 0.30468997835709644 1104 22-23 0.24852755451596664 1104 24-25 0.4127288885908982 1104 26-27 0.30111756606167717 1104 28-29 0.3121434807908301 1104 30-31 0.25353742811851276 1104 32-33 0.20113378418088956 1104 34-35 0.3008087359688787 1104 36-37 0.27148975458727165 1104 38-39 0.31660375267810537 1104 40-41 0.37194661093097636 1104 42-43 0.42536336301873945 1104 44-45 0.5281751400972965 1104 46-47 0.48072040593456933 1104 48-49 0.5555793486877931 1104 50-51 0.45731755380210615 1104 52-53 0.38642261951486034 1104 54-55 0.4193949869230451 1104 56-57 0.3998075135723198 1104 58-59 0.27992585969664674 1104 60-61 0.3723153203312606 1104 62-63 0.38139968146841596 1104 64-65 0.42241013984421727 1104 66-67 0.4674052951156682 1104 68-69 0.4072431626188191 1104 70-71 0.30496298026751134 1104 72-73 0.33685706620945055 1104 74-75 0.3322421469621588 1104 76-77 0.3642205941879908 1104 78-79 0.3627090018304209 1104 80-81 0.5197984634551247 1104 82-83 0.4730210492170599 1104 84-85 0.426792130076894 1104 86-87 0.41604437983031417 1104 88-89 0.5169544634574983 1104 90-91 0.5235452831370822 1104 92-93 0.636866613508829 1104 94-95 0.6757159068741316 1104 96-97 0.6931553652357181 1104 98-99 0.8377954414279785 1104 100-101 0.8933496771427478 1105 1 0.2954982642994288 1105 2 0.1427751393125618 1105 3 0.1415492755144001 1105 4 0.11666012297856554 1105 5 0.12883446020406808 1105 6 0.09614442258720146 1105 7 0.08392545327642154 1105 8 0.18032411714298036 1105 9 0.08831310154107541 1105 10-11 0.14246641640767876 1105 12-13 0.06988800631268788 1105 14-15 0.22418245112954338 1105 16-17 0.26603296263142084 1105 18-19 0.22052753566300254 1105 20-21 0.22516305837013562 1105 22-23 0.21443031724702877 1105 24-25 0.24861858403041737 1105 26-27 0.08080027013772195 1105 28-29 0.21327266097863884 1105 30-31 0.2530165908637443 1105 32-33 0.2373569660468604 1105 34-35 0.17450357539261319 1105 36-37 0.1719012934016675 1105 38-39 0.1801103015216441 1105 40-41 0.17268665855436893 1105 42-43 0.15617471745213862 1105 44-45 0.20988940923944455 1105 46-47 0.06949105433268699 1105 48-49 0.015405862685220484 1105 50-51 -0.04177239272127764 1105 52-53 -0.07830433795062675 1105 54-55 -0.040705784036006776 1105 56-57 0.15840714943939105 1105 58-59 0.09889465352094362 1105 60-61 0.16221238822178208 1105 62-63 0.13599756387561257 1105 64-65 0.1111426404514333 1105 66-67 0.2313307725138003 1105 68-69 0.27640767301507196 1105 70-71 0.2775479504282927 1105 72-73 0.3906499131082555 1105 74-75 0.19280618491697865 1105 76-77 0.16645943449514533 1105 78-79 0.2911663475528634 1105 80-81 0.2182601147469505 1105 82-83 0.2492872533138808 1105 84-85 0.4304987122160888 1105 86-87 0.431332846007475 1105 88-89 0.4375336489854291 1105 90-91 0.2715941825816799 1105 92-93 0.40849359448206 1105 94-95 0.5436528838234409 1105 96-97 0.6080914384058573 1105 98-99 0.40184671172431763 1105 100-101 0.38787436765903394 1106 1 0.09763046970325817 1106 2 0.10551096742523924 1106 3 0.0977451880737803 1106 4 0.08801875508565615 1106 5 0.0617279757370639 1106 6 0.12234425724587794 1106 7 0.09306838472320322 1106 8 0.0986234060906952 1106 9 0.09271462724827018 1106 10-11 -0.3687345599941878 1106 12-13 -0.07416641889361841 1106 14-15 -0.02995279997995226 1106 16-17 0.1182514859346071 1106 18-19 0.07059234016062277 1106 20-21 0.14523257784425425 1106 22-23 0.043783451014924424 1106 24-25 0.0337459518260701 1106 26-27 0.0030653987635460567 1106 28-29 -0.019830464860497443 1106 30-31 0.09025488852464747 1106 32-33 0.14645623638838856 1106 34-35 -0.0012811351860690934 1106 36-37 -0.00784753895448631 1106 38-39 0.1684190627091411 1106 40-41 0.23917707742611327 1106 42-43 0.10001945306459703 1106 44-45 0.09605470616667589 1106 46-47 0.18314484925446806 1106 48-49 0.4202013955092738 1106 50-51 0.47904406994690163 1106 52-53 0.3871805276091109 1106 54-55 0.09055556732277381 1106 56-57 0.18059462895673306 1106 58-59 0.30901464755850583 1106 60-61 0.12981423594283825 1106 62-63 0.22244285217633575 1106 64-65 0.28943878960537717 1106 66-67 -0.01673764644674236 1106 68-69 -0.3744773893896536 1106 70-71 -0.014443355408758407 1106 72-73 0.17313303539529556 1106 74-75 0.18586586637062652 1106 76-77 0.34816548425400384 1106 78-79 0.4707642689459597 1106 80-81 0.2868750481135507 1106 82-83 0.29010827238310455 1106 84-85 0.26129501465963045 1106 86-87 0.2664371146469584 1106 88-89 0.13613326925010227 1106 90-91 0.05880536384186641 1106 92-93 0.23348801791232177 1106 94-95 0.1780458358850261 1106 96-97 0.23447487699804626 1106 98-99 0.006032759833992429 1106 100-101 -0.12577752435742795 1107 1 0.3249985832340663 1107 2 0.20424632300125722 1107 3 0.1986872342823247 1107 4 0.1831924084918768 1107 5 0.235041205482446 1107 6 0.20959085795692545 1107 7 0.15112863606756832 1107 8 0.12918036460600035 1107 9 0.20327000124432715 1107 10-11 0.20852635090631821 1107 12-13 0.10954645631559146 1107 14-15 0.13328746760573296 1107 16-17 0.16141156199153528 1107 18-19 0.29756878034007883 1107 20-21 0.2741238105011945 1107 22-23 0.26702352053570877 1107 24-25 0.3018453705055819 1107 26-27 0.2906646787976115 1107 28-29 0.2606654649056921 1107 30-31 0.3526724440075739 1107 32-33 0.4218199330717951 1107 34-35 0.33789177052513963 1107 36-37 0.29788185981949056 1107 38-39 0.2830402140604349 1107 40-41 0.29804914730340926 1107 42-43 0.2936234420917998 1107 44-45 0.30868703828191 1107 46-47 0.2514741470153439 1107 48-49 0.2996194808158279 1107 50-51 0.3233390579144526 1107 52-53 0.20534666940378088 1107 54-55 0.2569588076646383 1107 56-57 0.32953436275749226 1107 58-59 0.2653065506651515 1107 60-61 0.16769377781870531 1107 62-63 0.10990732428385286 1107 64-65 0.07430291707995451 1107 66-67 0.06506699718497089 1107 68-69 0.05985320074472611 1107 70-71 0.2990428840319055 1107 72-73 0.24998915114442255 1107 74-75 0.16830444149169566 1107 76-77 0.408009557656861 1107 78-79 0.3643329776618067 1107 80-81 0.3688265073567436 1107 82-83 0.39587641760429193 1107 84-85 0.3509485991392616 1107 86-87 0.545428370081364 1107 88-89 0.40120724649244366 1107 90-91 0.5560469386559355 1107 92-93 0.5992630211308771 1107 94-95 0.6770052523858112 1107 96-97 0.5835941071197581 1107 98-99 0.46855850827175516 1107 100-101 0.46713781775017793 1108 1 0.24828187118396627 1108 2 0.24034998793381845 1108 3 0.25790536787560114 1108 4 0.14663526245612246 1108 5 0.18420611508643248 1108 6 0.27452765667700874 1108 7 0.24452490579699315 1108 8 0.22165589817031162 1108 9 0.2424014648510564 1108 10-11 0.2646992034931728 1108 12-13 0.12314082382599878 1108 14-15 0.31168477921153226 1108 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-0.8900777253634722 2316 70-71 -0.980677021336863 2316 72-73 -0.8416716154606512 2316 74-75 -0.8900914900560792 2316 76-77 -0.9143496611174236 2316 78-79 -0.8631572800413743 2316 80-81 -0.8435118165652753 2316 82-83 -0.9377412699169341 2316 84-85 -0.9819987838236592 2316 86-87 -0.8946943511997247 2316 88-89 -1.0052048253213783 2316 90-91 -0.8933846407761621 2316 92-93 -1.026933827948426 2316 94-95 -1.066815800185175 2316 96-97 -1.223715349671643 2316 98-99 -1.039902467857459 2316 100-101 -1.0189452320923884 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7471.0 3 1845.0 4 1968.0 5 2141.0 6 2605.0 7 3096.0 8 3922.0 9 5123.0 10 6506.0 11 8198.0 12 10583.0 13 13196.0 14 15639.0 15 19372.0 16 23762.0 17 31539.0 18 28064.0 19 22103.0 20 21107.0 21 21613.0 22 22969.0 23 25186.0 24 28136.0 25 31151.0 26 34945.0 27 40685.0 28 47606.0 29 56844.0 30 67641.0 31 82906.0 32 103937.0 33 134379.0 34 185593.0 35 273858.0 36 408394.0 37 550892.0 38 409503.0 39 48365.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.87986952442974 18.64684669694266 14.601831477661653 22.871452300965945 2 15.383835564214612 19.918655945490773 40.60723900688005 24.090269483414563 3 19.08219570766974 24.240360213320653 30.03538803860734 26.642056040402267 4 12.576571250266872 16.718469853116012 37.28858337742495 33.41637551919217 5 13.433701319128561 37.51416234104524 34.410605589380125 14.641530750446071 6 31.932876959904334 36.4041983688567 17.197368054479075 14.465556616759901 7 28.28674661801512 33.38523387376065 20.482171610963736 17.845847897260494 8 26.55925716239863 34.515554197487944 19.691143534975435 19.23404510513799 9 26.492312744744574 16.693070225852168 20.301635973570264 36.512981055833 10-11 24.868216428729934 26.713534552583905 27.070943987586006 21.347305031100156 12-13 25.984579607321812 24.135089697510427 27.57210428320033 22.30822641196743 14-15 22.662286746165176 25.47619051868823 26.258121990091276 25.60340074505532 16-17 21.739464926941128 28.61422250258512 27.233351950809876 22.412960619663874 18-19 21.781398660589897 26.77363669353234 29.959354317151277 21.48561032872649 20-21 22.397903958944674 25.99527940876389 30.29524493534404 21.3115716969474 22-23 22.000431629171636 26.00547908428211 30.231428565818362 21.762660720727894 24-25 21.805304836132166 26.173072287543185 30.35062627777629 21.670996598548356 26-27 21.391660469362346 26.49025383896672 30.086731318563814 22.031354373107114 28-29 21.60337057130091 26.88532942132659 29.963535579631124 21.547764427741377 30-31 21.680569388258235 26.404632483115275 29.840274161538872 22.074523967087618 32-33 21.330589849108367 26.41719312000994 29.87129117279309 22.380925858088602 34-35 22.205447046530672 26.624970923127982 29.561346710390428 21.608235319950918 36-37 22.296452251291814 26.113988952848423 29.606844649743312 21.98271414611645 38-39 21.750340193720806 26.540017935459115 29.559688940246364 22.14995293057371 40-41 22.161078808256683 26.30840837946752 29.69499680540832 21.83551600686748 42-43 21.925489382829564 26.400045544736102 29.36621787016966 22.30824720226467 44-45 21.940696537865723 26.657884378554087 29.54765328054682 21.85376580303337 46-47 22.347335187811904 26.931629054560318 28.805046170734915 21.915989586892863 48-49 22.108796863426395 27.013975718035283 28.611812435839123 22.265414982699202 50-51 22.29933087839452 27.235795641600347 28.57527006914263 21.889603410862506 52-53 22.576333330716267 26.90441658773827 28.518731188336822 22.000518893208636 54-55 22.082399890797262 27.167875123455303 28.662721450041083 22.08700353570635 56-57 22.19062735032212 27.359333081740132 28.33822691030167 22.111812657636076 58-59 22.073080363348087 26.923455057808702 28.435186931487983 22.568277647355227 60-61 21.827279102509426 27.081638880313776 28.786382040195786 22.304699976981016 62-63 21.900868472317335 27.024897966903342 28.949404359383458 22.124829201395865 64-65 21.74065330928076 27.18513522435091 28.66267336855572 22.41153809781261 66-67 21.621294197054393 27.24094907236835 28.629573057315422 22.50818367326184 68-69 22.190653186426694 26.829588584332146 28.732437790919253 22.247320438321907 70-71 21.994621411664387 26.925475909053347 28.339956479442847 22.739946199839416 72-73 21.88404219437896 27.088937627485237 28.360319009777662 22.66670116835814 74-75 21.960051350007692 27.486440678571007 28.073603819058672 22.479904152362625 76-77 22.11130217004848 27.100759630271515 28.247547434291793 22.54039076538821 78-79 22.062937611911966 27.332015189696357 28.13296408183481 22.472083116556867 80-81 22.280601744427226 27.28609266678621 27.88837235858087 22.544933230205693 82-83 22.22499381298864 27.354530292981526 27.94439736903475 22.47607852499508 84-85 22.1340929885338 27.5258856577612 27.86464020577207 22.47538114793293 86-87 22.408129622955908 27.462632778965173 27.622663322141307 22.506574275937616 88-89 22.243366296912065 27.469787748627787 27.501179650195347 22.7856663042648 90-91 22.12544157249785 27.527154745280136 27.569312960313447 22.778090721908566 92-93 22.299650324380128 27.147837213016608 27.778734475839013 22.77377798676426 94-95 22.1502564316748 26.90431798313016 27.952123472734925 22.993302112460114 96-97 22.415931645237986 26.564925494865648 27.90739925735798 23.11174360253839 98-99 22.097971107080113 26.83076379489257 27.59765480357508 23.47361029445224 100-101 22.535308917288503 26.165712468193387 27.878409383309034 23.42056923120908 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1284.0 1 869.5 2 640.0 3 845.5 4 711.5 5 694.0 6 1341.5 7 2970.5 8 4921.5 9 5656.0 10 4592.5 11 3430.0 12 3314.0 13 3423.0 14 3608.0 15 3786.0 16 3968.0 17 4272.5 18 4594.0 19 5090.5 20 5850.5 21 6851.0 22 8276.0 23 10228.0 24 12529.5 25 15391.0 26 19428.0 27 24164.5 28 27978.0 29 31683.0 30 35802.0 31 41923.5 32 51042.0 33 54904.5 34 55054.0 35 58503.0 36 63931.0 37 68944.0 38 72400.5 39 75184.5 40 78580.5 41 82893.0 42 85249.0 43 87369.5 44 90486.5 45 95885.0 46 99512.5 47 101119.5 48 102362.5 49 99902.5 50 97085.5 51 94699.5 52 93958.5 53 95417.0 54 96996.5 55 92368.5 56 84116.0 57 76215.5 58 70071.5 59 62264.0 60 50012.0 61 41159.0 62 34615.0 63 25514.0 64 16907.0 65 12114.0 66 9056.5 67 7177.5 68 5630.5 69 4198.0 70 3171.0 71 2142.0 72 1487.5 73 1186.5 74 820.5 75 527.5 76 393.0 77 259.0 78 156.0 79 105.5 80 59.5 81 40.5 82 34.5 83 23.0 84 15.0 85 12.5 86 8.0 87 3.5 88 2.0 89 2.5 90 3.5 91 2.0 92 3.0 93 4.0 94 2.0 95 1.0 96 1.0 97 0.5 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.5094107917452475 2 0.050377366433974606 3 0.057691360852504925 4 0.06636112009007987 5 0.08152427925044757 6 0.11031644264436934 7 0.0704997705903214 8 0.05280347189963344 9 0.05194719938234209 10-11 0.07622609304970733 12-13 0.10871093167444804 14-15 0.05628207900112957 16-17 0.06116996795400104 18-19 0.06020666137204827 20-21 0.07626177107126114 22-23 0.06530861845424259 24-25 0.0917281934148362 26-27 0.0676812068875707 28-29 0.0676633678767938 30-31 0.07190905244169675 32-33 0.07235502771111933 34-35 0.07422812384269417 36-37 0.0693223958790458 38-39 0.04044103743123953 40-41 0.04409803464050469 42-43 0.04299201597233669 44-45 0.021941983255590925 46-47 0.028560256253822008 48-49 0.02881000240469865 50-51 0.026383896939039822 52-53 0.025973599691171044 54-55 0.025634658486409886 56-57 0.025099488163102788 58-59 0.022459314568121117 60-61 0.028934875480136978 62-63 0.02030079426411583 64-65 0.023226392031527956 66-67 0.016679475076404483 68-69 0.01862392725108693 70-71 0.042153582465822244 72-73 0.0374976006530505 74-75 0.032074541376871935 76-77 0.022387958525013504 78-79 0.01498476905259868 80-81 0.01760710363680345 82-83 0.021567364029275955 84-85 0.01862392725108693 86-87 0.009133573517774433 88-89 0.04167192917484585 90-91 0.053302964201386735 92-93 0.03437577376709245 94-95 0.05405220265401667 96-97 0.04377693244652043 98-99 0.03384060344378535 100-101 0.1671872090011367 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2802846.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.69274263577951 #Duplication Level Percentage of deduplicated Percentage of total 1 72.0753855884386 35.816235864208515 2 14.668771502192138 14.578629740829815 3 5.5075117000141995 8.210500844170504 4 2.664601154326612 5.296453575558133 5 1.4193698388760148 3.526619005412681 6 0.8917225956892076 2.658728487005584 7 0.5545628890920984 1.9290425646105587 8 0.38968938059830244 1.5491787278374218 9 0.2959077226742989 1.3234019676114197 >10 1.3852644815729123 12.281007194192362 >50 0.09170496644814088 3.1117022566405863 >100 0.04897356041539512 4.5112246946477566 >500 0.0033032143347629175 1.2000127019866846 >1k 0.0030159783055948622 2.775276930120818 >5k 7.180900727606815E-5 0.27030527870066084 >10k+ 1.436180145521363E-4 0.9616801664663774 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15197 0.5421988935531956 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11753 0.4193237873218864 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7575 0.27026101327008334 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3682 0.1313664753611151 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3667 0.130831305037808 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3549 0.12662129849445883 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3405 0.12148366339071073 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3090 0.11024508660126171 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2806 0.1001125284799807 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 3.567802155380638E-5 0.0 0.0 0.0 0.0 3 3.567802155380638E-5 0.0 0.0 0.0 0.0 4 3.567802155380638E-5 0.0 0.0 0.0 0.0 5 7.135604310761276E-5 0.0 0.0 0.0 0.0 6 1.0703406466141914E-4 0.0 0.0 0.0 0.0 7 1.783901077690319E-4 0.0 0.0 0.0 0.0 8 1.783901077690319E-4 0.0 0.0 0.0 0.0 9 1.783901077690319E-4 0.0 0.0 0.0 0.0 10-11 1.783901077690319E-4 0.0 0.0 0.0 7.135604310761276E-5 12-13 3.2110219398425745E-4 0.0 0.0 0.0 1.0703406466141914E-4 14-15 4.994923017532894E-4 0.0 0.0 0.0 1.2487307543832232E-4 16-17 5.173313125301925E-4 1.783901077690319E-5 0.0 3.567802155380638E-5 1.6055109699212873E-4 18-19 6.065263664147085E-4 3.567802155380638E-5 0.0 5.351703233070957E-5 1.783901077690319E-4 20-21 6.778824095223212E-4 3.567802155380638E-5 0.0 2.319071400997415E-4 2.1406812932283828E-4 22-23 7.135604310761276E-4 3.567802155380638E-5 0.0 3.2110219398425745E-4 2.1406812932283828E-4 24-25 8.205944957375467E-4 3.567802155380638E-5 0.0 8.027554849606435E-4 2.1406812932283828E-4 26-27 8.205944957375467E-4 3.567802155380638E-5 0.0 0.0012487307543832234 2.1406812932283828E-4 28-29 9.633065819527722E-4 3.567802155380638E-5 0.0 0.004210006543349153 2.1406812932283828E-4 30-31 0.001034662625060385 3.567802155380638E-5 0.0 0.014538793783176102 2.1406812932283828E-4 32-33 0.0011060186681679978 3.567802155380638E-5 0.0 0.03624886989866728 2.1406812932283828E-4 34-35 0.001213052732829417 3.567802155380638E-5 0.0 0.06789527501689355 2.1406812932283828E-4 36-37 0.0013914428405984488 3.567802155380638E-5 0.0 0.11845103155863718 2.1406812932283828E-4 38-39 0.001498476905259868 3.567802155380638E-5 0.0 0.19041360103266464 2.319071400997415E-4 40-41 0.0015341549268136744 3.567802155380638E-5 0.0 0.2685306292247237 2.6758516165354784E-4 42-43 0.0016768670130288999 3.567802155380638E-5 0.0 0.3476823200418432 2.8542417243045104E-4 44-45 0.0018195790992441255 3.567802155380638E-5 0.0 0.4461001424980181 2.8542417243045104E-4 46-47 0.00206932525012077 3.567802155380638E-5 0.0 0.5609655328904978 2.8542417243045104E-4 48-49 0.0021406812932283827 3.567802155380638E-5 0.0 0.6772580441451297 3.2110219398425745E-4 50-51 0.0021406812932283827 3.567802155380638E-5 0.0 0.8042004448335727 3.567802155380638E-4 52-53 0.002158520304005286 3.567802155380638E-5 0.0 0.9286810620348032 3.567802155380638E-4 54-55 0.002176359314782189 3.567802155380638E-5 0.0 1.0622595747322543 3.9245823709187016E-4 56-57 0.0022833933794436084 3.567802155380638E-5 0.0 1.2094670916632593 4.2813625864567657E-4 58-59 0.0022833933794436084 3.567802155380638E-5 0.0 1.3684661947177976 4.2813625864567657E-4 60-61 0.0022833933794436084 3.567802155380638E-5 0.0 1.5331737812209447 4.4597526942257977E-4 62-63 0.0022833933794436084 3.567802155380638E-5 0.0 1.6961153056571785 4.63814280199483E-4 64-65 0.0023190714009974148 3.567802155380638E-5 0.0 1.867030867910688 4.63814280199483E-4 66-67 0.002354749422551221 3.567802155380638E-5 0.0 2.059406760128812 4.63814280199483E-4 68-69 0.002497461508766447 3.567802155380638E-5 0.0 2.270014121360931 4.63814280199483E-4 70-71 0.002586656562650963 3.567802155380638E-5 0.0 2.4877035698714804 5.351703233070957E-4 72-73 0.002640173594981672 3.567802155380638E-5 0.0 2.7107090435935475 5.351703233070957E-4 74-75 0.002658012605758575 3.567802155380638E-5 0.0 2.9351594771885434 5.351703233070957E-4 76-77 0.0026936906273123816 3.567802155380638E-5 0.0 3.1752012062025528 5.351703233070957E-4 78-79 0.002729368648866188 3.567802155380638E-5 0.0 3.43846932724809 5.351703233070957E-4 80-81 0.002818563702750704 3.567802155380638E-5 0.0 3.7177390409605096 5.351703233070957E-4 82-83 0.002818563702750704 3.567802155380638E-5 0.0 4.008140297397716 5.351703233070957E-4 84-85 0.00290775875663522 3.567802155380638E-5 0.0 4.304196520251201 5.351703233070957E-4 86-87 0.002996953810519736 3.567802155380638E-5 0.0 4.611205895721706 5.351703233070957E-4 88-89 0.002996953810519736 3.567802155380638E-5 0.0 4.931166393016241 5.351703233070957E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6790 0.0 50.494564 1 GTATCAA 11955 0.0 41.14126 1 TATCAAC 14925 0.0 32.669212 2 ATCAACG 14955 0.0 32.44479 3 TCAACGC 15145 0.0 32.100517 4 CAACGCA 15375 0.0 31.589401 5 AACGCAG 15905 0.0 30.536755 6 ACGCAGA 17935 0.0 26.90646 7 CGCAGAG 17865 0.0 26.772373 8 GCAGAGT 19310 0.0 24.29941 9 GTACATG 10685 0.0 23.998812 1 TAAGGTG 1690 0.0 23.9022 5 TACATGG 10415 0.0 23.773006 2 TGGTATC 3165 0.0 23.423756 2 ACATGGG 10340 0.0 23.393915 3 GAGTACT 11950 0.0 23.21272 12-13 GTGGTAT 3335 0.0 23.052603 1 GTACTTT 12545 0.0 21.699707 14-15 CAGAGTA 18185 0.0 21.698685 10-11 AGAGTAC 17190 0.0 21.226145 10-11 >>END_MODULE