FastQCFastQC Report
Thu 26 May 2016
SRR938109_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938109_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2802846
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141970.5065208719993891No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118450.4226061653048366No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78240.27914484063698114No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA37150.1325438500723907No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA36330.1296182523049786No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA35850.12790570727039588No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC33500.11952137220525139No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA32490.11591789202831694No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG28720.10246727790253192No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28550.1018607515361172No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69800.053.4981841
GTATCAA119650.040.6989361
TATCAAC150600.032.0193862
ATCAACG152400.031.572663
TCAACGC153600.031.2928374
CAACGCA156250.030.7621145
AACGCAG162100.029.6812466
ACGACGG1503.3431206E-828.497516
CGACGGT1658.4262865E-825.9068287
GTGGTAT30650.025.885581
ACGCAGA184600.025.857717
CGCAGAG184500.025.743018
TGGTATC30450.025.4315152
TACATGG105800.023.1705282
GCAGAGT201150.023.1635259
GTACATG110050.023.0959421
GAGTACT127650.022.9572612-13
ACATGGG107300.022.0010473
GTACTTT134300.021.67904514-15
TAAGGTG18050.021.5770655
Produced by FastQC (version 0.11.5)