##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938109_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2802846 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.535093972340974 34.0 31.0 34.0 31.0 34.0 2 32.749840697633765 34.0 31.0 34.0 31.0 34.0 3 32.80739220064178 34.0 31.0 34.0 31.0 34.0 4 36.25993793451371 37.0 35.0 37.0 35.0 37.0 5 36.22923307238428 37.0 37.0 37.0 35.0 37.0 6 36.2624200544732 37.0 37.0 37.0 35.0 37.0 7 36.2271623200133 37.0 37.0 37.0 35.0 37.0 8 36.217050811924736 37.0 37.0 37.0 35.0 37.0 9 37.934850148741674 39.0 38.0 39.0 35.0 39.0 10-11 37.92523224608131 39.0 38.0 39.0 35.0 39.0 12-13 37.87609469089632 39.0 38.0 39.0 35.0 39.0 14-15 39.20718904998705 40.5 39.0 41.0 36.0 41.0 16-17 39.164875451594554 40.0 39.0 41.0 36.0 41.0 18-19 39.20344339289422 40.0 39.0 41.0 36.0 41.0 20-21 39.14114011258556 40.0 39.0 41.0 36.0 41.0 22-23 39.007226048095404 40.0 38.5 41.0 35.0 41.0 24-25 38.966009548865685 40.0 38.5 41.0 35.0 41.0 26-27 38.92964187115525 40.0 38.0 41.0 35.0 41.0 28-29 38.87112099630161 40.0 38.0 41.0 35.0 41.0 30-31 38.66700221845938 40.0 38.0 41.0 35.0 41.0 32-33 38.48202576952141 40.0 38.0 41.0 34.0 41.0 34-35 38.284656738186825 40.0 38.0 41.0 34.0 41.0 36-37 38.14796442615827 40.0 38.0 41.0 34.0 41.0 38-39 38.11278946470837 40.0 38.0 41.0 34.0 41.0 40-41 38.0399877481674 40.0 38.0 41.0 33.5 41.0 42-43 37.87785879067205 40.0 38.0 41.0 33.0 41.0 44-45 37.66084401354908 40.0 37.0 41.0 33.0 41.0 46-47 37.330471956004715 40.0 37.0 41.0 32.5 41.0 48-49 36.9742438578502 40.0 37.0 41.0 31.5 41.0 50-51 36.680576100149636 40.0 36.0 41.0 31.0 41.0 52-53 36.28195716068596 39.0 35.5 41.0 30.0 41.0 54-55 36.066704877827746 39.0 35.0 41.0 29.5 41.0 56-57 35.827361902865874 39.0 35.0 41.0 29.0 41.0 58-59 35.50745652811464 38.5 35.0 40.0 28.0 41.0 60-61 35.168874779420634 38.0 35.0 40.0 28.0 41.0 62-63 34.817095373773654 37.5 34.0 40.0 27.0 41.0 64-65 34.438443639072574 37.0 34.0 40.0 26.0 41.0 66-67 34.03853333361876 36.5 34.0 39.5 26.0 41.0 68-69 33.5990245272127 36.0 33.5 39.0 25.5 40.5 70-71 33.10283708059594 35.5 33.0 38.5 24.5 40.0 72-73 32.70536340562414 35.0 33.0 38.0 23.5 40.0 74-75 32.27458697338348 35.0 32.0 37.0 22.5 39.0 76-77 30.956433567880644 34.0 30.5 35.5 21.0 38.5 78-79 31.660137053551995 35.0 32.0 36.0 22.0 39.0 80-81 31.506358180221103 35.0 32.0 36.0 21.5 37.5 82-83 31.152760979375962 35.0 32.0 35.5 20.0 37.0 84-85 30.78160305632204 34.5 31.0 35.0 18.5 37.0 86-87 30.376917426073355 34.0 31.0 35.0 16.0 36.0 88-89 29.938497869665333 34.0 30.5 35.0 9.0 36.0 90-91 29.621099589488686 34.0 30.0 35.0 4.5 35.5 92-93 29.250475231247094 34.0 30.0 35.0 2.0 35.0 94-95 28.877122217917073 34.0 29.5 35.0 2.0 35.0 96-97 28.347701586173482 34.0 29.0 35.0 2.0 35.0 98-99 27.710298032785246 34.0 29.0 35.0 2.0 35.0 100-101 25.34742971964924 31.0 22.5 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09038224720278265 1101 2 0.11992012307605648 1101 3 0.09303598091919696 1101 4 0.026859352564734706 1101 5 0.023890364140989107 1101 6 0.10262084105391267 1101 7 0.1364098337848887 1101 8 0.103149697578381 1101 9 0.009120282237873312 1101 10-11 0.050672102292686816 1101 12-13 0.1293967979638211 1101 14-15 0.14727940022041253 1101 16-17 0.08451156504735025 1101 18-19 0.18704688550521809 1101 20-21 0.2144839358392403 1101 22-23 0.24924461526848063 1101 24-25 0.25140263490297343 1101 26-27 0.1884578781378181 1101 28-29 0.20364735512782772 1101 30-31 0.15249354114045843 1101 32-33 0.17477237875357332 1101 34-35 0.1700127410194341 1101 36-37 0.24801323504305373 1101 38-39 0.23055966373647863 1101 40-41 0.33604638943214127 1101 42-43 0.2877405079179738 1101 44-45 0.2865554344898271 1101 46-47 0.23906516428497326 1101 48-49 0.2472918850116912 1101 50-51 0.3434341709288873 1101 52-53 0.24513626051279402 1101 54-55 0.32002618282060524 1101 56-57 0.16818734864485663 1101 58-59 0.16024099785114032 1101 60-61 0.1760778703787622 1101 62-63 0.1379034003346149 1101 64-65 0.2262975365186577 1101 66-67 0.1500592871071902 1101 68-69 0.23390676413416855 1101 70-71 0.3330471312624752 1101 72-73 0.09932968252663699 1101 74-75 0.2696124642316491 1101 76-77 0.16485759396598 1101 78-79 0.2893932072989607 1101 80-81 0.36251765911254097 1101 82-83 0.44557116543559516 1101 84-85 0.3208613034487726 1101 86-87 0.3400667717451036 1101 88-89 0.2782049167944258 1101 90-91 0.37040753174896324 1101 92-93 0.6163633986052695 1101 94-95 0.4938916357611518 1101 96-97 0.3285052143032203 1101 98-99 0.4797830293017533 1101 100-101 0.4710545153688699 1102 1 0.17348669460201904 1102 2 0.1442362767339347 1102 3 0.11168092326772694 1102 4 0.09341389158448976 1102 5 0.14574864008745436 1102 6 0.054105622785371565 1102 7 0.07528306669947682 1102 8 0.13271137439938485 1102 9 0.04733001055159036 1102 10-11 0.10355103751003014 1102 12-13 0.08323361420016795 1102 14-15 0.2582397950155695 1102 16-17 0.24298811746950832 1102 18-19 0.2376200926205243 1102 20-21 0.10392227224863859 1102 22-23 0.05927640130643397 1102 24-25 0.10046046498494121 1102 26-27 -0.01952848793413864 1102 28-29 0.023288546657724396 1102 30-31 0.060416401288513555 1102 32-33 0.0522557249865514 1102 34-35 0.22043793302562875 1102 36-37 0.16108371338819438 1102 38-39 0.10636374134600857 1102 40-41 0.20679562703028864 1102 42-43 0.22951864071151107 1102 44-45 0.20272860720609742 1102 46-47 0.17303322609740945 1102 48-49 0.2523725243046613 1102 50-51 0.30543034072784536 1102 52-53 0.25413425610706497 1102 54-55 0.08989493724451592 1102 56-57 0.2823271741358653 1102 58-59 0.12907794639149728 1102 60-61 0.12226784570406579 1102 62-63 0.14786175764805165 1102 64-65 0.28199405096955843 1102 66-67 0.1809496269300297 1102 68-69 0.3357046102249157 1102 70-71 0.3820457553007941 1102 72-73 0.32581711033826366 1102 74-75 0.28716986763318886 1102 76-77 0.23862418985000033 1102 78-79 0.39597781240457763 1102 80-81 0.40861568315217767 1102 82-83 0.45950707737404883 1102 84-85 0.31771147577474323 1102 86-87 0.42549662746758443 1102 88-89 0.24320967973871532 1102 90-91 0.3629002715665166 1102 92-93 0.32132926417116536 1102 94-95 0.3294620644922901 1102 96-97 0.32971496907774167 1102 98-99 0.41164365364436506 1102 100-101 0.5405414073492629 1103 1 0.15731251389262724 1103 2 0.17875603447555477 1103 3 0.11228940385722552 1103 4 0.05674657423980278 1103 5 0.11319670019722139 1103 6 0.11776866949181652 1103 7 0.12106406393764502 1103 8 0.08063748314507535 1103 9 0.10597542774894464 1103 10-11 0.05295953253041574 1103 12-13 0.15571528086361752 1103 14-15 0.17046171874193305 1103 16-17 0.18579142523312697 1103 18-19 0.1834066947090136 1103 20-21 0.22501393603235442 1103 22-23 0.2011771993211653 1103 24-25 0.21776176820927162 1103 26-27 0.15702719625380723 1103 28-29 0.19201805593696974 1103 30-31 0.18901180002850992 1103 32-33 0.32434318384405003 1103 34-35 0.23347710191008275 1103 36-37 0.29282765290219714 1103 38-39 0.23294606467887746 1103 40-41 0.2840061445641311 1103 42-43 0.19296954061210414 1103 44-45 0.1648330545581942 1103 46-47 0.17319605666278193 1103 48-49 0.2007317204789345 1103 50-51 0.3615033248983366 1103 52-53 0.3182307123599202 1103 54-55 0.409244652202446 1103 56-57 0.42418980118718963 1103 58-59 0.4453794602808969 1103 60-61 0.44042717420433775 1103 62-63 0.40779168413431677 1103 64-65 0.3944420047804442 1103 66-67 0.42771604704770283 1103 68-69 0.5119231980989838 1103 70-71 0.5543557653701896 1103 72-73 0.4032484396476974 1103 74-75 0.20185663702967105 1103 76-77 0.23433358029353712 1103 78-79 0.302653833373153 1103 80-81 0.3141427098048517 1103 82-83 0.4258175783510332 1103 84-85 0.45169864388868675 1103 86-87 0.5009704466963427 1103 88-89 0.33921190750566055 1103 90-91 0.4167036134746276 1103 92-93 0.5636709126542776 1103 94-95 0.5063778764192861 1103 96-97 0.4644402702096002 1103 98-99 0.49900603685249223 1103 100-101 0.6769691012368213 1104 1 0.1083801335670529 1104 2 0.11222018518728305 1104 3 0.052594156888645216 1104 4 0.032357123049848724 1104 5 0.026887926043627886 1104 6 0.02200881372497321 1104 7 0.032586285287145245 1104 8 0.053110762183329996 1104 9 0.0535451044792552 1104 10-11 0.08393544461824831 1104 12-13 0.13808495326043158 1104 14-15 0.15969840069319474 1104 16-17 0.12716691263845803 1104 18-19 0.19914698655183116 1104 20-21 0.17829186382729745 1104 22-23 0.13912674299511707 1104 24-25 0.11613296094055414 1104 26-27 0.1274380025456736 1104 28-29 0.16821475507776285 1104 30-31 0.07660590683652657 1104 32-33 0.026056087998881594 1104 34-35 0.13454792656513348 1104 36-37 0.09513271108387755 1104 38-39 0.147849991998946 1104 40-41 0.13914824257153668 1104 42-43 0.19408767258688187 1104 44-45 0.20425296209224086 1104 46-47 0.22802605275361287 1104 48-49 0.3247369351178335 1104 50-51 0.2883588958317773 1104 52-53 0.22365782794054923 1104 54-55 0.19475154923048166 1104 56-57 0.2629187871222953 1104 58-59 0.22568977463080842 1104 60-61 0.22992088657402832 1104 62-63 0.29294391168614453 1104 64-65 0.2160832527908667 1104 66-67 0.15647018530966506 1104 68-69 0.2804732648618895 1104 70-71 0.2721380361395944 1104 72-73 0.32780883818478657 1104 74-75 0.29228584261879575 1104 76-77 0.24459380666448283 1104 78-79 0.34708457013049454 1104 80-81 0.3003114127627313 1104 82-83 0.37021680698893533 1104 84-85 0.45406716971496053 1104 86-87 0.4204152898825093 1104 88-89 0.23423457258699187 1104 90-91 0.31699373193251645 1104 92-93 0.4693009351071993 1104 94-95 0.34161275635890576 1104 96-97 0.3788697701152586 1104 98-99 0.4649989353376647 1104 100-101 0.5612119719605388 1105 1 0.045992182245640834 1105 2 0.05451621371781812 1105 3 0.03019934008030134 1105 4 0.05891710417673579 1105 5 0.0025490498474312062 1105 6 0.04587251568364792 1105 7 -0.06454543585584815 1105 8 -0.001078481533930642 1105 9 -0.010797223980475223 1105 10-11 -0.020994892440349133 1105 12-13 -0.032602080645659726 1105 14-15 0.0810627809742499 1105 16-17 0.06664521928924927 1105 18-19 0.00964797468558487 1105 20-21 0.11127619227977448 1105 22-23 0.12810327234367946 1105 24-25 0.02858845169878066 1105 26-27 -0.01764818273937152 1105 28-29 -0.08585392433104744 1105 30-31 0.10418323210193847 1105 32-33 0.08688145784143586 1105 34-35 0.07236005594526773 1105 36-37 0.020932399487818998 1105 38-39 -0.0719400529334635 1105 40-41 -0.020379237920252535 1105 42-43 0.03445492315456988 1105 44-45 0.046520003321134595 1105 46-47 0.03850208308556802 1105 48-49 0.11452148375668969 1105 50-51 0.16569541316712844 1105 52-53 0.05508830317334912 1105 54-55 0.18452582194758804 1105 56-57 0.15336456413674426 1105 58-59 0.14323358951586584 1105 60-61 0.07327170988869369 1105 62-63 0.06122459521201051 1105 64-65 0.05334437246757773 1105 66-67 -0.012077379380535547 1105 68-69 0.1617713800877354 1105 70-71 0.2936721573586638 1105 72-73 0.12100577364558518 1105 74-75 0.17120418264332926 1105 76-77 0.146393570917823 1105 78-79 0.26004632780668757 1105 80-81 0.24179519730244792 1105 82-83 0.30714683841903323 1105 84-85 0.23271851703191615 1105 86-87 0.20370732432818173 1105 88-89 -0.004284838587356177 1105 90-91 -0.0016656688069609515 1105 92-93 -0.005137326123186625 1105 94-95 -0.155474152284107 1105 96-97 -0.029156477684203708 1105 98-99 0.15759515463503604 1105 100-101 0.35119471900861754 1106 1 -0.128917324035001 1106 2 -0.02507940833135791 1106 3 -0.04998433256923818 1106 4 -0.010023569578606839 1106 5 -0.03099705649114526 1106 6 -0.07657071959368267 1106 7 -0.051833764068959454 1106 8 -0.13088886759113194 1106 9 -0.15128423030869698 1106 10-11 -0.024715072328341137 1106 12-13 -0.12146101933193165 1106 14-15 -0.2648325465474741 1106 16-17 -0.1896555116378238 1106 18-19 -0.13331082635720293 1106 20-21 -0.019604710162496986 1106 22-23 -0.07034636198135047 1106 24-25 -0.04938811507945928 1106 26-27 0.0011081206344840666 1106 28-29 0.029608555662022695 1106 30-31 -0.0714090736061479 1106 32-33 -0.07070394560620485 1106 34-35 -0.02517071297648954 1106 36-37 -0.037902922029175556 1106 38-39 -0.07123241001999503 1106 40-41 0.06275499513490956 1106 42-43 -0.06428498351849754 1106 44-45 -0.04978392025818579 1106 46-47 -0.06643640550017693 1106 48-49 -0.0994795120970906 1106 50-51 -0.054505486061344754 1106 52-53 -0.12355882629398707 1106 54-55 -0.38667732693783563 1106 56-57 -0.23267691179890448 1106 58-59 -0.19436731732422885 1106 60-61 -0.3327922943315329 1106 62-63 -0.17620592231480003 1106 64-65 -0.3434146890618166 1106 66-67 -0.2878715251980495 1106 68-69 -0.37240400646633276 1106 70-71 -0.46857449194222767 1106 72-73 -0.308113910641282 1106 74-75 -0.36139020674606925 1106 76-77 -0.2214141077279841 1106 78-79 -0.17426802369375238 1106 80-81 -0.5118746348824459 1106 82-83 -0.5110954426569947 1106 84-85 -0.2964731076474969 1106 86-87 0.027609823688592883 1106 88-89 0.03266826113968335 1106 90-91 -0.2340414619484683 1106 92-93 -0.06838567006697716 1106 94-95 -0.19431945348230073 1106 96-97 0.09712005463030238 1106 98-99 0.24342873816518207 1106 100-101 0.20188761440591207 1107 1 0.1089682835786121 1107 2 0.13777277582939007 1107 3 0.07462176584920854 1107 4 0.0876882855963288 1107 5 0.0552582293660322 1107 6 0.0639065673132393 1107 7 0.11000383261147562 1107 8 0.15088668525753945 1107 9 0.04283497505490175 1107 10-11 0.08720298822010619 1107 12-13 0.10638950603522801 1107 14-15 0.1824524787553159 1107 16-17 0.13143344921464717 1107 18-19 0.18858908649423967 1107 20-21 0.1485485574132852 1107 22-23 0.13753352650810768 1107 24-25 0.15022293496163996 1107 26-27 0.13170865342907945 1107 28-29 0.06290475182549926 1107 30-31 0.15894103087308764 1107 32-33 0.11200102681325319 1107 34-35 0.15965107812445467 1107 36-37 0.01240222171642813 1107 38-39 0.10978814416561278 1107 40-41 0.05413372494466273 1107 42-43 0.11165929092023674 1107 44-45 0.11264712839645341 1107 46-47 0.138021664159254 1107 48-49 0.1263820702434444 1107 50-51 0.10460884195394726 1107 52-53 0.045190740439032595 1107 54-55 0.08208300957443981 1107 56-57 0.1437782463064039 1107 58-59 0.14038725549928444 1107 60-61 0.06684561826242685 1107 62-63 0.058016970027019 1107 64-65 0.0809513794488339 1107 66-67 0.012120304624581024 1107 68-69 0.1128418911143072 1107 70-71 0.16408183078472405 1107 72-73 0.21071389172955435 1107 74-75 0.1293643710290624 1107 76-77 0.01791056521734191 1107 78-79 0.14966267339568518 1107 80-81 0.2905203412012156 1107 82-83 0.32091271266550336 1107 84-85 0.40248061448724215 1107 86-87 0.4210008929419864 1107 88-89 0.515186309804232 1107 90-91 0.30559318257454393 1107 92-93 0.48187131914838943 1107 94-95 0.25412338498585996 1107 96-97 0.5303763902314742 1107 98-99 0.612079148654697 1107 100-101 0.5135886700918633 1108 1 0.060018106459629905 1108 2 0.09251013469045688 1108 3 0.07391399627694284 1108 4 0.030063339897516528 1108 5 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-0.5343626614332706 2316 50-51 -0.5181164684468555 2316 52-53 -0.5123115476336295 2316 54-55 -0.6020230879233637 2316 56-57 -0.6061685092353315 2316 58-59 -0.5081242021325139 2316 60-61 -0.33266366929793634 2316 62-63 -0.47179992659814474 2316 64-65 -0.4999839386076559 2316 66-67 -0.3413877856549945 2316 68-69 -0.41459080501237366 2316 70-71 -0.5227777247247758 2316 72-73 -0.401820717168313 2316 74-75 -0.3282576451227115 2316 76-77 -0.31464050629140417 2316 78-79 -0.272879878999742 2316 80-81 -0.42484054110262903 2316 82-83 -0.41114534394055013 2316 84-85 -0.3966381177765932 2316 86-87 -0.4737172567986967 2316 88-89 -0.5595219969068701 2316 90-91 -0.493482378506922 2316 92-93 -0.6258813617820351 2316 94-95 -0.5068944763713255 2316 96-97 -0.5709020523825075 2316 98-99 -0.49202758202044095 2316 100-101 -0.48207665539007394 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 3.0 4 10.0 5 50.0 6 90.0 7 204.0 8 514.0 9 1129.0 10 2033.0 11 3228.0 12 5016.0 13 7367.0 14 9968.0 15 13519.0 16 17579.0 17 25202.0 18 24934.0 19 17093.0 20 13975.0 21 13390.0 22 13856.0 23 15065.0 24 16808.0 25 19532.0 26 23360.0 27 28199.0 28 34685.0 29 42172.0 30 51403.0 31 64360.0 32 81082.0 33 106375.0 34 149668.0 35 231590.0 36 391709.0 37 662072.0 38 629471.0 39 86114.0 40 15.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.1388310310306 19.01345989041139 15.012954689626188 22.83475438893182 2 15.628934184044876 20.16382620362837 40.12332462638633 24.08391498594042 3 19.09384916967751 24.242983237982962 30.247558913969158 26.41560867837037 4 12.81579508827813 17.009889234014285 36.958612781437154 33.215702896270436 5 13.43077714580109 37.57437975543429 34.43978013775998 14.555062961004635 6 32.100729044692436 36.48223983765073 17.08277943204871 14.334251685608129 7 28.319607998441583 33.307680835836145 20.330407022005488 18.042304143716777 8 26.569198582005626 34.74132591607346 19.58240807868613 19.107067423234785 9 26.321535529345613 16.66418109073732 20.23295673884827 36.7813266410688 10-11 24.934174050233228 26.75428831980066 26.998058402067045 21.31347922789907 12-13 25.942060320117484 24.055656286503076 27.606636254721096 22.39564713865835 14-15 22.550275684072545 25.47136018175811 26.23775976275543 25.740604371413912 16-17 21.736997502351635 28.58580941128746 27.24705847094542 22.43013461541549 18-19 21.72937792515179 26.778923993683563 29.95387545373524 21.537822627429406 20-21 22.33215809930335 25.937475694347818 30.27950875645683 21.450857449892002 22-23 21.981710732591086 25.9649834489658 30.311065966521173 21.74223985192194 24-25 21.828886451948296 26.16321543429011 30.338019359584145 21.669878754177454 26-27 21.41954420351594 26.47825175417858 30.081640117941465 22.020563924364012 28-29 21.6163181309851 26.89791499388746 29.973200449329084 21.512566425798354 30-31 21.713233620398693 26.4127961364984 29.83567416832748 22.038296074775424 32-33 21.32579025372177 26.400725123214574 29.909013519887374 22.364471103176285 34-35 22.197746160320268 26.556511303545378 29.623776600841207 21.62196593529315 36-37 22.27537840554285 26.129864736274804 29.620368873822716 21.97438798435963 38-39 21.767246839677767 26.45792333151936 29.60934224260213 22.165487586200744 40-41 22.13924977956418 26.238124923503154 29.78590159944876 21.836723697483908 42-43 21.96406990771813 26.31781672006334 29.43444840147163 22.2836649707469 44-45 21.91321222448665 26.625268306188737 29.605197433394416 21.856322035930194 46-47 22.40440609294981 26.848050160444064 28.790914663167367 21.956629083438763 48-49 22.077631093538496 26.949482775721535 28.662705692713764 22.310180438026205 50-51 22.297853681579365 27.1745754137045 28.615503670198077 21.912067234518055 52-53 22.571058131627638 26.882390256189602 28.54041927383809 22.00613233834467 54-55 22.109705634915368 27.101685215670074 28.6700910431754 22.118518106239158 56-57 22.186805839493143 27.330577562948516 28.328135045592944 22.154481551965397 58-59 22.091242258761273 26.937923096738103 28.42840812516992 22.542426519330707 60-61 21.78937051839452 27.146247064590778 28.67904622658541 22.3853361904293 62-63 21.885647659557463 27.089411976255562 28.84384300814244 22.181097356044535 64-65 21.69919431891727 27.26696365051808 28.56100549227464 22.472836538290007 66-67 21.603184762915976 27.381882557942888 28.48477939922493 22.5301532799162 68-69 22.1455103200719 26.95395632785187 28.563520773044758 22.337012579031477 70-71 21.936149899066876 27.063848673812263 28.225632089668856 22.774369337452004 72-73 21.907643159845385 27.217264166493628 28.194984669154138 22.680108004506845 74-75 21.99466185441512 27.621371277622814 27.864998647802985 22.51896822015908 76-77 22.03549535008345 27.30494290446211 28.172008023273488 22.487553722180955 78-79 22.02058193707396 27.49883511259627 28.069790491521836 22.41079245880794 80-81 22.21106689414973 27.47475244804745 27.819081034063238 22.495099623739584 82-83 22.209102611490326 27.623371118763657 27.828716118342655 22.33881015140336 84-85 22.02180001783962 27.81593078226742 27.706341985549905 22.455927214343056 86-87 22.299499096718627 27.695497043798312 27.522993224899135 22.48201063458393 88-89 22.11330705526244 27.838277834941938 27.295900164261727 22.752514945533893 90-91 21.952630104875954 27.8810026276966 27.510199494440453 22.656167772986993 92-93 22.085517357517897 27.580543910102435 27.671362686594588 22.66257604578508 94-95 21.88015324423818 27.357675020318634 27.831568341607067 22.930603393836122 96-97 22.127641735450943 27.032158396201 27.826190888186826 23.01400898016123 98-99 21.729645510313446 27.445318080265558 27.514069627799742 23.310966781621254 100-101 21.88366752936123 27.148084482700796 27.788308740473077 23.179939247464898 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1293.0 1 854.5 2 605.0 3 835.0 4 691.0 5 661.5 6 1339.0 7 3007.0 8 4846.5 9 5479.5 10 4477.5 11 3364.0 12 3159.5 13 3201.0 14 3450.5 15 3680.5 16 3836.5 17 4097.5 18 4446.5 19 4929.0 20 5674.0 21 6637.0 22 7932.0 23 9795.5 24 12030.0 25 14921.5 26 19065.0 27 23884.0 28 27773.5 29 31608.0 30 36193.0 31 42501.5 32 51601.5 33 55059.5 34 54991.0 35 58452.5 36 63744.5 37 68879.5 38 72269.0 39 75317.5 40 78807.0 41 83244.5 42 85914.5 43 88028.0 44 91338.0 45 96892.0 46 100328.0 47 102684.0 48 104312.0 49 101456.5 50 98592.5 51 96165.0 52 95248.0 53 96240.5 54 97842.5 55 92790.0 56 84089.0 57 75982.5 58 69415.0 59 61431.5 60 49097.5 61 40282.0 62 33727.5 63 24332.0 64 15464.0 65 10987.0 66 8310.5 67 6467.5 68 5012.5 69 3820.5 70 2797.0 71 1737.5 72 1202.0 73 1015.0 74 696.5 75 426.5 76 308.0 77 190.5 78 100.5 79 58.0 80 32.0 81 14.0 82 7.0 83 6.0 84 5.5 85 3.0 86 2.5 87 3.0 88 1.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0173395184751499 3 0.007135604310761276 4 0.0 5 0.0 6 0.0 7 0.0 8 4.994923017532893E-4 9 2.8542417243045104E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.3894120476116066E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005405220265401666 26-27 0.02171007611549118 28-29 1.783901077690319E-5 30-31 0.0 32-33 4.2813625864567657E-4 34-35 0.02003320910246228 36-37 0.0035321241338268313 38-39 0.018588249229533123 40-41 0.015805363548336226 42-43 0.008455691108252112 44-45 0.0027828856811968976 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.4974615087664464E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 7.135604310761276E-5 84-85 0.0034250900691654125 86-87 3.3894120476116066E-4 88-89 7.135604310761276E-5 90-91 3.924582370918702E-4 92-93 4.1029724786877336E-4 94-95 0.0 96-97 3.567802155380638E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2802846.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.50510074065852 #Duplication Level Percentage of deduplicated Percentage of total 1 70.03291723849418 33.26920788576866 2 15.439478388712532 14.669079524780177 3 6.095364006248218 8.686826435034165 4 2.8575015107308857 5.429835885354185 5 1.59422001166625 3.78667911284895 6 0.9607571016383 2.738451774037832 7 0.6076016559087701 2.020492451189594 8 0.42476450658259685 1.6142784541009894 9 0.3065440319220289 1.3106164606113258 >10 1.5170813159743182 12.914639007436055 >50 0.10212616218721204 3.3485842316369507 >100 0.054445295836721586 4.835335688836747 >500 0.0034494125886211304 1.1609101009715346 >1k 0.003524399818763257 3.0086626228795073 >5k 7.498723018645228E-5 0.27871246831491336 >10k+ 1.4997446037290455E-4 0.9276878961984882 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14197 0.5065208719993891 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11845 0.4226061653048366 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7824 0.27914484063698114 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3715 0.1325438500723907 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3633 0.1296182523049786 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3585 0.12790570727039588 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3350 0.11952137220525139 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3249 0.11591789202831694 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2872 0.10246727790253192 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2855 0.1018607515361172 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 7.135604310761276E-5 0.0 0.0 0.0 0.0 3 7.135604310761276E-5 0.0 0.0 0.0 0.0 4 7.135604310761276E-5 0.0 0.0 0.0 0.0 5 1.0703406466141914E-4 0.0 0.0 0.0 0.0 6 1.783901077690319E-4 0.0 0.0 0.0 0.0 7 3.2110219398425745E-4 0.0 0.0 0.0 0.0 8 3.2110219398425745E-4 0.0 0.0 0.0 0.0 9 3.2110219398425745E-4 0.0 0.0 0.0 0.0 10-11 3.2110219398425745E-4 0.0 0.0 0.0 0.0 12-13 3.567802155380638E-4 0.0 0.0 0.0 3.567802155380638E-5 14-15 4.63814280199483E-4 0.0 0.0 0.0 3.567802155380638E-5 16-17 4.994923017532893E-4 0.0 0.0 3.567802155380638E-5 3.567802155380638E-5 18-19 6.243653771916117E-4 0.0 0.0 5.351703233070957E-5 3.567802155380638E-5 20-21 6.778824095223212E-4 0.0 0.0 2.319071400997415E-4 3.567802155380638E-5 22-23 6.778824095223212E-4 0.0 0.0 3.2110219398425745E-4 5.351703233070957E-5 24-25 7.135604310761276E-4 0.0 3.567802155380638E-5 7.670774634068372E-4 7.135604310761276E-5 26-27 7.49238452629934E-4 0.0 3.567802155380638E-5 0.0012665697651601266 7.135604310761276E-5 28-29 0.0011060186681679978 0.0 3.567802155380638E-5 0.004227845554126056 7.135604310761276E-5 30-31 0.0011773747112756106 0.0 3.567802155380638E-5 0.014770700923275842 8.919505388451595E-5 32-33 0.0011773747112756106 0.0 3.567802155380638E-5 0.03649861604954393 1.0703406466141914E-4 34-35 0.0013200867974908362 0.0 3.567802155380638E-5 0.06855531841563896 1.0703406466141914E-4 36-37 0.0015341549268136744 0.0 3.567802155380638E-5 0.11941433814058997 1.0703406466141914E-4 38-39 0.0016411889914750935 0.0 3.567802155380638E-5 0.19189423892714763 1.0703406466141914E-4 40-41 0.001694706023805803 0.0 3.567802155380638E-5 0.2710816077658209 1.0703406466141914E-4 42-43 0.001855257120797932 0.0 3.567802155380638E-5 0.35103605406790095 1.2487307543832232E-4 44-45 0.0020514862393438667 0.0 3.567802155380638E-5 0.4505063781599132 1.4271208621522552E-4 46-47 0.002443944476435737 0.0 3.567802155380638E-5 0.5662280410696842 1.4271208621522552E-4 48-49 0.002533139530320253 0.0 3.567802155380638E-5 0.6829486885829618 1.4271208621522552E-4 50-51 0.002533139530320253 0.0 3.567802155380638E-5 0.8105332936593734 1.4271208621522552E-4 52-53 0.0025509785410971564 0.0 3.567802155380638E-5 0.9360307344748873 1.4271208621522552E-4 54-55 0.002640173594981672 0.0 3.567802155380638E-5 1.0703228076034144 1.783901077690319E-4 56-57 0.002640173594981672 0.0 3.567802155380638E-5 1.2185114701271493 1.783901077690319E-4 58-59 0.002640173594981672 0.0 3.567802155380638E-5 1.3783668456989788 1.783901077690319E-4 60-61 0.002640173594981672 0.0 3.567802155380638E-5 1.5447869772367087 1.783901077690319E-4 62-63 0.002640173594981672 0.0 3.567802155380638E-5 1.7089772324273258 1.9622911854593508E-4 64-65 0.002640173594981672 0.0 3.567802155380638E-5 1.8813734325753182 2.1406812932283828E-4 66-67 0.0027472076596430912 0.0 3.567802155380638E-5 2.0751229286232635 2.6758516165354784E-4 68-69 0.0027472076596430912 0.0 3.567802155380638E-5 2.2874249958791886 3.2110219398425745E-4 70-71 0.0027472076596430912 0.0 3.567802155380638E-5 2.506595082284221 3.2110219398425745E-4 72-73 0.0027650466704199944 0.0 3.567802155380638E-5 2.7315271691701932 3.3894120476116066E-4 74-75 0.0027828856811968976 0.0 3.567802155380638E-5 2.958492903284733 3.567802155380638E-4 76-77 0.0027828856811968976 0.0 3.567802155380638E-5 3.201156966882947 3.567802155380638E-4 78-79 0.0027828856811968976 0.0 3.567802155380638E-5 3.468492382385618 3.567802155380638E-4 80-81 0.003211021939842574 0.0 3.567802155380638E-5 3.7531316383418853 3.567802155380638E-4 82-83 0.003211021939842574 0.0 3.567802155380638E-5 4.049062988119932 3.924582370918702E-4 84-85 0.0032288609506194773 0.0 3.567802155380638E-5 4.350007099926289 3.924582370918702E-4 86-87 0.0032645389721732837 0.0 3.567802155380638E-5 4.662457373683749 3.924582370918702E-4 88-89 0.003282377982950187 0.0 3.567802155380638E-5 4.989357246170499 3.924582370918702E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6980 0.0 53.498184 1 GTATCAA 11965 0.0 40.698936 1 TATCAAC 15060 0.0 32.019386 2 ATCAACG 15240 0.0 31.57266 3 TCAACGC 15360 0.0 31.292837 4 CAACGCA 15625 0.0 30.762114 5 AACGCAG 16210 0.0 29.681246 6 ACGACGG 150 3.3431206E-8 28.49751 6 CGACGGT 165 8.4262865E-8 25.906828 7 GTGGTAT 3065 0.0 25.88558 1 ACGCAGA 18460 0.0 25.85771 7 CGCAGAG 18450 0.0 25.74301 8 TGGTATC 3045 0.0 25.431515 2 TACATGG 10580 0.0 23.170528 2 GCAGAGT 20115 0.0 23.163525 9 GTACATG 11005 0.0 23.095942 1 GAGTACT 12765 0.0 22.95726 12-13 ACATGGG 10730 0.0 22.001047 3 GTACTTT 13430 0.0 21.679045 14-15 TAAGGTG 1805 0.0 21.577065 5 >>END_MODULE