FastQCFastQC Report
Thu 26 May 2016
SRR938108_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938108_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2767369
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140440.507485629852759No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111640.40341566303590165No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71190.25724794922541955No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA36550.13207490580403264No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA36050.13026813554679553No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA33570.12130655507089946No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA32320.11678962942780671No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC30720.11100796460464794No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64450.051.8587951
GTATCAA117850.042.5813371
ATCAACG147250.033.7505763
TATCAAC148400.033.6172182
TCAACGC149200.033.341344
CAACGCA150450.033.0011025
AACGCAG156150.031.8504436
TGGTATC28100.028.094542
ACGCAGA175550.027.9193297
CGCAGAG175800.027.7143638
GTGGTAT30350.026.6684931
GCAGAGT191450.024.9026479
TAAGGTG17850.023.4457765
GAGTACT119100.022.90909612-13
AGGTAAG16200.022.3109862
GGTAAGG16900.022.231083
GTACATG105150.022.0962621
TACATGG102000.021.8205592
CAGAGTA180850.021.69635210-11
AGAGTAC172800.021.18044910-11
Produced by FastQC (version 0.11.5)