##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938108_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2767369 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.96122418080133 33.0 31.0 34.0 30.0 34.0 2 32.081372957491396 33.0 31.0 34.0 30.0 34.0 3 32.103108042331904 34.0 31.0 34.0 30.0 34.0 4 35.62690266458864 37.0 35.0 37.0 33.0 37.0 5 35.63760524888441 37.0 35.0 37.0 33.0 37.0 6 35.66186150094187 37.0 35.0 37.0 35.0 37.0 7 35.59783787416857 37.0 35.0 37.0 33.0 37.0 8 35.58784896412441 37.0 35.0 37.0 33.0 37.0 9 37.22418549893418 39.0 37.0 39.0 34.0 39.0 10-11 37.1327475663708 39.0 37.0 39.0 34.0 39.0 12-13 36.98848220096417 39.0 37.0 39.0 33.0 39.0 14-15 38.16325054591563 40.0 38.0 41.0 33.0 41.0 16-17 37.990572814828816 40.0 38.0 41.0 33.0 41.0 18-19 38.186048734375504 40.0 38.0 41.0 33.5 41.0 20-21 38.189876919196536 40.0 38.0 41.0 33.5 41.0 22-23 38.08152057062141 40.0 38.0 41.0 33.0 41.0 24-25 37.95759564409372 40.0 38.0 41.0 33.0 41.0 26-27 37.778764053510756 40.0 38.0 41.0 32.5 41.0 28-29 37.59833130312582 40.0 37.5 41.0 32.0 41.0 30-31 37.359297224186584 40.0 37.0 41.0 31.5 41.0 32-33 37.055170994543914 40.0 36.5 41.0 31.0 41.0 34-35 36.80617944336299 39.0 36.0 41.0 30.5 41.0 36-37 36.52653657679912 39.0 35.5 41.0 30.0 41.0 38-39 36.21698461607397 39.0 35.0 40.5 30.0 41.0 40-41 36.08658187614301 39.0 35.0 40.5 29.5 41.0 42-43 35.85458769683407 39.0 35.0 40.5 28.5 41.0 44-45 35.51147678535099 39.0 35.0 40.0 27.0 41.0 46-47 35.10334256111129 38.0 35.0 40.0 25.5 41.0 48-49 34.66521034961366 38.0 34.0 40.0 24.0 41.0 50-51 33.92240246963813 37.5 33.5 39.5 23.0 40.5 52-53 33.98260965559707 38.0 33.5 39.5 23.0 40.5 54-55 34.058310980573964 38.0 34.0 40.0 22.5 41.0 56-57 33.73975245079352 38.0 33.0 40.0 20.5 41.0 58-59 33.33073977485475 37.5 33.0 40.0 18.5 41.0 60-61 32.8312915624913 37.0 32.0 40.0 14.0 41.0 62-63 32.29013152926119 36.0 31.5 39.0 9.5 41.0 64-65 31.743570156347054 35.5 31.0 39.0 7.5 40.0 66-67 31.16261600820129 35.0 30.5 38.5 2.0 40.0 68-69 30.485367329040688 35.0 29.0 38.0 2.0 40.0 70-71 29.88896132030098 34.5 29.0 37.5 2.0 39.5 72-73 29.194305493774053 34.0 28.0 36.5 2.0 39.0 74-75 28.50624221056173 34.0 26.5 36.0 2.0 39.0 76-77 27.752933923882214 33.5 26.0 35.5 2.0 38.0 78-79 26.96661251173949 33.0 24.5 35.0 2.0 37.0 80-81 26.235704201355148 32.0 22.5 35.0 2.0 36.5 82-83 25.475523864002234 31.5 19.5 35.0 2.0 36.0 84-85 24.730578574812395 31.0 17.0 34.5 2.0 35.5 86-87 23.95728271148517 31.0 8.5 34.0 2.0 35.0 88-89 23.03723825770976 30.0 2.0 34.0 2.0 35.0 90-91 22.108198436854643 29.0 2.0 34.0 2.0 35.0 92-93 21.136083586973765 29.0 2.0 34.0 2.0 35.0 94-95 20.009573894916073 27.0 2.0 33.5 2.0 35.0 96-97 18.701666637156087 25.5 2.0 33.0 2.0 35.0 98-99 17.090410241641067 19.5 2.0 32.5 2.0 34.0 100-101 13.180725447166605 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1609680360801775 1101 2 0.1757127574253019 1101 3 0.12686205809380624 1101 4 -0.022340081855141136 1101 5 0.03556564292623676 1101 6 0.042950146560045255 1101 7 0.11090605755663319 1101 8 0.18240905055590417 1101 9 0.1958471051236046 1101 10-11 0.19251712577088398 1101 12-13 0.1841580116783561 1101 14-15 0.19576393854961083 1101 16-17 0.16809146165918065 1101 18-19 0.2498157288940277 1101 20-21 0.22563473852783034 1101 22-23 0.19875169097844747 1101 24-25 0.21309939659035138 1101 26-27 0.35779650307626554 1101 28-29 0.28349625287177105 1101 30-31 0.21556322120630256 1101 32-33 0.12828867888720907 1101 34-35 0.2241519807485517 1101 36-37 0.30285262867496954 1101 38-39 0.2306254638120393 1101 40-41 0.3949483505211333 1101 42-43 0.3855183498469259 1101 44-45 0.24441387256390357 1101 46-47 0.3926244827405796 1101 48-49 0.43865925963646646 1101 50-51 0.5437564747416914 1101 52-53 0.4639918635442868 1101 54-55 0.3336136516913655 1101 56-57 0.38280636816775626 1101 58-59 0.24838175032451915 1101 60-61 0.14849683393057234 1101 62-63 -0.02011393262805683 1101 64-65 -0.04704115416786436 1101 66-67 -0.049279159660851946 1101 68-69 -0.13355169795192268 1101 70-71 0.023071337062695818 1101 72-73 -0.17010808673206768 1101 74-75 -0.24785051632932564 1101 76-77 -0.22533592658800572 1101 78-79 -0.2929634611380898 1101 80-81 -0.3939308270313404 1101 82-83 -0.32977611250518635 1101 84-85 -0.16704363939189548 1101 86-87 -0.14547559004669708 1101 88-89 -0.1598170111939048 1101 90-91 -0.335627411056862 1101 92-93 -0.6468619377415656 1101 94-95 -0.6554230353861321 1101 96-97 -0.8380802037796755 1101 98-99 -0.9467950755834771 1101 100-101 -1.241493087243235 1102 1 0.1866265213920073 1102 2 0.3292301756514391 1102 3 0.25071549562080975 1102 4 0.2078037752506745 1102 5 0.12851687400939227 1102 6 0.2936530305802094 1102 7 0.30658064456487466 1102 8 0.35444112696929864 1102 9 0.3371182397023915 1102 10-11 0.37601385693251643 1102 12-13 0.281421076009309 1102 14-15 0.3137532193698931 1102 16-17 0.45019794448786143 1102 18-19 0.40761253007070053 1102 20-21 0.46112143126055116 1102 22-23 0.5522357771057713 1102 24-25 0.3327529014869839 1102 26-27 0.2769458820234334 1102 28-29 0.2978062167002662 1102 30-31 0.3232645232744815 1102 32-33 0.28537298440675585 1102 34-35 0.22644175484862927 1102 36-37 0.3028504241710763 1102 38-39 0.06601295386972339 1102 40-41 0.09821948157271265 1102 42-43 0.3698370082387683 1102 44-45 0.23441538926258687 1102 46-47 0.18289706523752614 1102 48-49 0.09153155184433359 1102 50-51 0.17141682594561303 1102 52-53 0.03170946130521912 1102 54-55 0.041787334853012226 1102 56-57 0.14097837590644957 1102 58-59 0.22796072535366108 1102 60-61 0.21652332677651742 1102 62-63 -0.002520316165885106 1102 64-65 0.009475976590959334 1102 66-67 -0.10701203022472683 1102 68-69 -0.1453438537980034 1102 70-71 -0.39048398099430415 1102 72-73 -0.3268510468693471 1102 74-75 -0.264026019529382 1102 76-77 -0.5889313666157996 1102 78-79 -0.5131381989343957 1102 80-81 -0.5774578921664961 1102 82-83 -0.7773101544542484 1102 84-85 -0.5920286046753667 1102 86-87 -0.5785206624514245 1102 88-89 -0.6401550410023269 1102 90-91 -0.7564940103538902 1102 92-93 -0.8322221801311791 1102 94-95 -0.8903931687674351 1102 96-97 -0.8675134170116365 1102 98-99 -1.3425402948963416 1102 100-101 -1.476403787199672 1103 1 0.3235105909498799 1103 2 0.3538585100803715 1103 3 0.395008200888185 1103 4 0.3195995551117434 1103 5 0.27171595172998764 1103 6 0.2474364094155561 1103 7 0.34902385588174667 1103 8 0.25825026724776023 1103 9 0.24118400738352364 1103 10-11 0.3912451867954658 1103 12-13 0.3594545599631829 1103 14-15 0.30656469100960493 1103 16-17 0.36965888875825925 1103 18-19 0.3524050665562086 1103 20-21 0.33165882878231656 1103 22-23 0.3700278536360102 1103 24-25 0.35292430885141357 1103 26-27 0.24181062951193866 1103 28-29 0.27399958347412934 1103 30-31 0.3065728847874709 1103 32-33 0.39953269745783615 1103 34-35 0.29947100445036057 1103 36-37 0.2153350512026293 1103 38-39 0.22866883034114238 1103 40-41 0.45450862958948335 1103 42-43 0.34968583495801653 1103 44-45 0.46889486005739656 1103 46-47 0.5243447596377209 1103 48-49 0.7276738695056295 1103 50-51 0.7209552914321904 1103 52-53 0.5431200039537316 1103 54-55 0.4699025447643592 1103 56-57 0.6094872325129259 1103 58-59 0.6567584654532723 1103 60-61 0.3405799743145792 1103 62-63 0.5513215934578355 1103 64-65 0.5604569841891056 1103 66-67 0.19258516345592014 1103 68-69 0.13278547062048673 1103 70-71 0.1839399398381154 1103 72-73 0.2811742842293512 1103 74-75 0.24462183042649954 1103 76-77 0.19688937716937716 1103 78-79 -0.014449959773447318 1103 80-81 -0.1181974923410003 1103 82-83 -0.22473805695952365 1103 84-85 -0.35578235291599114 1103 86-87 -0.5024567938130708 1103 88-89 -0.527695484493961 1103 90-91 -0.5960091030062529 1103 92-93 -0.8655985479058828 1103 94-95 -0.9607006873581163 1103 96-97 -1.0993412358168975 1103 98-99 -1.1446085143562001 1103 100-101 -1.3167558799961423 1104 1 0.17059968647222945 1104 2 0.18030477588792593 1104 3 0.20915842843609056 1104 4 0.19523732310290853 1104 5 0.278778480093095 1104 6 0.24905965182159662 1104 7 0.2540177836506743 1104 8 0.27859637523241787 1104 9 0.2553880674186928 1104 10-11 0.3038849813133666 1104 12-13 0.3826356006388707 1104 14-15 0.42287180075622643 1104 16-17 0.4274098482810089 1104 18-19 0.4185404819023262 1104 20-21 0.3826940477225307 1104 22-23 0.45626961111054953 1104 24-25 0.564573714213708 1104 26-27 0.5071253290880762 1104 28-29 0.43347557106142176 1104 30-31 0.4478583604884463 1104 32-33 0.5094377832499148 1104 34-35 0.45396932425208547 1104 36-37 0.4142483882472803 1104 38-39 0.3744354472173441 1104 40-41 0.3519802481100598 1104 42-43 0.36772477072833 1104 44-45 0.4711946831267326 1104 46-47 0.2993805407875243 1104 48-49 0.16988157104215418 1104 50-51 0.13045240781141842 1104 52-53 0.28381644568143827 1104 54-55 0.13131411342099142 1104 56-57 0.1129505171030516 1104 58-59 0.0623026604554866 1104 60-61 0.1096707564657109 1104 62-63 0.2100893071939467 1104 64-65 -0.04732282072783889 1104 66-67 0.020456704075563437 1104 68-69 0.03487924203635728 1104 70-71 -0.12698676897589678 1104 72-73 -0.1680445016551424 1104 74-75 -0.14707979061116205 1104 76-77 -0.08983108523882777 1104 78-79 -0.5631991352912422 1104 80-81 -0.731099651336077 1104 82-83 -0.6154792366075341 1104 84-85 -0.4653039052024006 1104 86-87 -0.36610397238443326 1104 88-89 -0.47303851502414673 1104 90-91 -0.3901600103500691 1104 92-93 -0.3198868658886873 1104 94-95 -0.5319345720610578 1104 96-97 -0.8878043642376952 1104 98-99 -0.9796165341107006 1104 100-101 -1.3887246792009655 1105 1 0.14973986059649746 1105 2 0.18817916349913588 1105 3 0.2306768194395019 1105 4 0.10731076600780654 1105 5 0.09851590585964942 1105 6 0.1410330959723467 1105 7 0.14443361841891544 1105 8 0.18692413843175615 1105 9 0.14033184310903124 1105 10-11 0.10014737069658963 1105 12-13 0.10029224454113717 1105 14-15 0.0737574593054191 1105 16-17 0.10922727942502064 1105 18-19 0.19934904516122742 1105 20-21 0.08701887649705498 1105 22-23 0.15299790707256022 1105 24-25 0.2252436861645748 1105 26-27 0.1362880445088379 1105 28-29 0.18679983282672197 1105 30-31 0.07761582714804405 1105 32-33 0.0739989682383424 1105 34-35 -0.049220283688711675 1105 36-37 0.06840774999829335 1105 38-39 -0.03170207265477387 1105 40-41 -0.057683912648215596 1105 42-43 0.14764189524697002 1105 44-45 0.08951925056128118 1105 46-47 0.3249215970935424 1105 48-49 0.21808039686660408 1105 50-51 0.10885209455113909 1105 52-53 0.23270948838850813 1105 54-55 0.10882649012835088 1105 56-57 0.16583281879541545 1105 58-59 0.05808087553827335 1105 60-61 -0.09686293103832355 1105 62-63 -0.04033240048021014 1105 64-65 -0.1516991641876615 1105 66-67 -0.2256166774057462 1105 68-69 -0.1283196721569233 1105 70-71 -0.43927289104136236 1105 72-73 -0.30918706144825236 1105 74-75 -0.5137573657587176 1105 76-77 -0.446221658688831 1105 78-79 -0.4381129756762654 1105 80-81 -0.5653171076289958 1105 82-83 -0.5384100223687369 1105 84-85 -0.5857848170992526 1105 86-87 -0.5345441726137281 1105 88-89 -0.5956994571286494 1105 90-91 -0.6852956246424107 1105 92-93 -0.7741799688024571 1105 94-95 -0.9911901645769987 1105 96-97 -1.0484518093787578 1105 98-99 -1.185041554891864 1105 100-101 -1.288873182305318 1106 1 0.19035916196577318 1106 2 0.07940631106509244 1106 3 0.10244318907947303 1106 4 0.12118805760400164 1106 5 0.13856485026541776 1106 6 0.09539725642037666 1106 7 0.18878836278634736 1106 8 0.07669882031558473 1106 9 0.23483864393018905 1106 10-11 0.21813727533184846 1106 12-13 0.26256445619517166 1106 14-15 0.2591534545349319 1106 16-17 -0.27037347999729633 1106 18-19 0.12442969078485788 1106 20-21 0.2972673125863139 1106 22-23 0.26583414613781287 1106 24-25 0.21685009554514068 1106 26-27 0.16411382548029962 1106 28-29 -0.00323866045783916 1106 30-31 0.03079767061426253 1106 32-33 0.04464022703366766 1106 34-35 0.013247671500231206 1106 36-37 0.038515606498897625 1106 38-39 0.061229545093567594 1106 40-41 0.009865735600669723 1106 42-43 -0.12730370701573435 1106 44-45 0.0741097734628724 1106 46-47 0.10983628294089698 1106 48-49 0.21557685363436718 1106 50-51 0.14225717830498041 1106 52-53 0.1692105211824071 1106 54-55 0.08610254522287875 1106 56-57 0.028767177542050604 1106 58-59 0.08798959828111208 1106 60-61 0.2513098581466764 1106 62-63 0.14476013261590737 1106 64-65 0.014720027572671768 1106 66-67 -0.044286625419747594 1106 68-69 -0.21238581063265372 1106 70-71 -0.29219199223564374 1106 72-73 -0.15514830351558828 1106 74-75 -0.15039194668270284 1106 76-77 -0.6600550850157099 1106 78-79 -0.6285975590716397 1106 80-81 -0.7391997479680334 1106 82-83 -0.4427124599978569 1106 84-85 -0.6011280546970106 1106 86-87 -0.8318657880890115 1106 88-89 -1.3359812701789302 1106 90-91 -1.051591231313342 1106 92-93 -0.7858553199454086 1106 94-95 -1.3455049436709778 1106 96-97 -1.2913009260227213 1106 98-99 -1.5180042827383158 1106 100-101 -1.7364692152487393 1107 1 0.147520121181536 1107 2 0.04766720258444934 1107 3 0.1611389143319144 1107 4 0.11402810405895991 1107 5 0.08319979390496002 1107 6 0.12460910735662623 1107 7 0.1227193130129649 1107 8 0.101226355509489 1107 9 0.13730138383012047 1107 10-11 0.13777898087624862 1107 12-13 0.05650604262033454 1107 14-15 0.08068741040892036 1107 16-17 0.18533143680420494 1107 18-19 0.24976495538104615 1107 20-21 0.16025979762396503 1107 22-23 0.2187013836784999 1107 24-25 0.29457515023463543 1107 26-27 0.20316092504859995 1107 28-29 0.25296833217460346 1107 30-31 0.2144000196922704 1107 32-33 0.2578054272261383 1107 34-35 0.21781206628407546 1107 36-37 0.23920289452566124 1107 38-39 0.12393698226059513 1107 40-41 0.2421109707257827 1107 42-43 0.13175917947301485 1107 44-45 0.0318154123781369 1107 46-47 0.13046976266144128 1107 48-49 0.28236527444018833 1107 50-51 0.1288398431017086 1107 52-53 0.0010528128851419183 1107 54-55 -0.09999302331371496 1107 56-57 -0.14487277236852236 1107 58-59 -0.15363826332679054 1107 60-61 -0.06792209635818836 1107 62-63 0.07727913522123231 1107 64-65 -0.06042059402747668 1107 66-67 -0.2125422283770071 1107 68-69 -0.15958619917318373 1107 70-71 -0.27373765327285327 1107 72-73 -0.30889671958420806 1107 74-75 -0.4566453337887104 1107 76-77 -0.5261799178669087 1107 78-79 -0.7268366788748715 1107 80-81 -0.7764616061971559 1107 82-83 -0.6300902235352766 1107 84-85 -0.688053238281146 1107 86-87 -0.7551064585942946 1107 88-89 -0.7876194796462208 1107 90-91 -0.7868911033329518 1107 92-93 -0.8179451472977668 1107 94-95 -0.9421200107562449 1107 96-97 -1.0839729762393056 1107 98-99 -0.9630431825036077 1107 100-101 -1.1659479275907678 1108 1 -0.09517884218430339 1108 2 -0.10780636794496701 1108 3 -0.09431894961930709 1108 4 0.0025279080133486787 1108 5 0.0593732587302469 1108 6 0.17026801550868242 1108 7 0.1133223080395922 1108 8 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8623.0 3 2458.0 4 2289.0 5 2579.0 6 3042.0 7 3846.0 8 4891.0 9 6281.0 10 8160.0 11 10289.0 12 13322.0 13 16626.0 14 19912.0 15 24257.0 16 28857.0 17 36686.0 18 35710.0 19 29932.0 20 30228.0 21 32436.0 22 35399.0 23 39125.0 24 44062.0 25 48991.0 26 55855.0 27 64241.0 28 74485.0 29 86106.0 30 101317.0 31 120119.0 32 145225.0 33 181429.0 34 229761.0 35 296710.0 36 362423.0 37 370112.0 38 180884.0 39 10701.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.88556142882113 18.64796196237274 14.574636973925667 22.89183963488047 2 15.427515678075643 19.860257682663818 40.60116313333731 24.11106350592323 3 19.061331939743653 24.258593883453823 30.003966519104434 26.67610765769809 4 12.589117207762134 16.754288335957764 37.197696195350126 33.45889826092998 5 13.463115458904843 37.44528097637094 34.399299075148484 14.692304489575733 6 32.002758980263515 36.36947887135259 17.22718862807715 14.400573520306747 7 28.246237350759046 33.40894464846348 20.455376293880082 17.889441706897387 8 26.502744294261827 34.58620786917068 19.69899912272264 19.212048713844855 9 26.483067863854064 16.637132538890622 20.328150811458904 36.55164878579642 10-11 24.92623458721573 26.70302516868288 27.038256730766104 21.33248351333529 12-13 26.019536375248375 24.098958292808224 27.596057403641318 22.285447928302084 14-15 22.64579942107397 25.472654800474242 26.22152433152028 25.66002144693151 16-17 21.749197481803467 28.572361604395937 27.229189571958333 22.449251341842267 18-19 21.79820042461078 26.770144814529033 29.92908981865845 21.502564942201737 20-21 22.38530108358139 25.975866419144616 30.26367756230462 21.375154934969377 22-23 22.018897520911004 25.97428858228767 30.24061346794581 21.766200428855516 24-25 21.810718439795416 26.14643068726815 30.343902046870454 21.698948826065983 26-27 21.369890973415202 26.460361651101177 30.091076843349786 22.078670532133838 28-29 21.62244206630117 26.892832494068465 29.94409021547112 21.54063522415925 30-31 21.63363125909051 26.40936583375504 29.838918250614295 22.11808465654016 32-33 21.334888135809077 26.43255866373338 29.847793496856532 22.384759703601002 34-35 22.182879663130116 26.625908265747285 29.547314817594522 21.64389725352808 36-37 22.31414284605999 26.097554491858705 29.545165613548917 22.04313704853239 38-39 21.766999361509352 26.50889512194593 29.535344398094214 22.1887611184505 40-41 22.163737076093422 26.32025019710489 29.6715485040729 21.844464222728792 42-43 21.939509757482185 26.373333526180858 29.35326171458325 22.333895001753707 44-45 21.959756659430024 26.654782934203443 29.501944241050104 21.883516165316433 46-47 22.37441907087132 26.91335354710911 28.749368884415418 21.962858497604156 48-49 22.065816380111748 27.052990864067965 28.576869316026105 22.304323439794185 50-51 22.267329653714242 27.240166371038942 28.517469754500034 21.975034220746775 52-53 22.56608491602665 26.918895160544093 28.45560530790229 22.059414615526972 54-55 22.098486245331657 27.119907301959167 28.580660672386177 22.200945780322996 56-57 22.18003626851424 27.3703845207109 28.238764947568974 22.21081426320589 58-59 22.137195797085074 26.906175573381542 28.3511829171845 22.60544571234889 60-61 21.821982124756534 27.065858024700283 28.695679836199027 22.416480014344152 62-63 21.945745650673114 27.016343407329902 28.880824854980258 22.15708608701673 64-65 21.76703967262717 27.196797848362326 28.512615385727685 22.52354709328282 66-67 21.65196730985955 27.228613181432447 28.49943651712455 22.619982991583456 68-69 22.245506308036703 26.812726646198527 28.60407456033937 22.3376924854254 70-71 22.016512348106083 26.87460489368714 28.230742629035827 22.878140129170955 72-73 21.89977468683205 27.03598936776373 28.26911980355237 22.79511614185185 74-75 22.013351437958402 27.439352398139793 27.954401506212257 22.59289465768955 76-77 22.15590494262221 27.090087647962413 28.148206379325924 22.605801030089456 78-79 22.125157407286927 27.29319465588707 28.005015346758675 22.57663259006733 80-81 22.36067074096404 27.25821329481691 27.699673824880396 22.681442139338657 82-83 22.337639571731536 27.276732668346753 27.809999015080116 22.575628744841595 84-85 22.21425669658347 27.46403405542319 27.687994436862436 22.6337148111309 86-87 22.519576264616123 27.26336751329527 27.482402641867264 22.734653580221345 88-89 22.346756061525376 27.35892720384096 27.290224974489778 23.004091760143886 90-91 22.22899095445757 27.38505866670043 27.378402478706533 23.007547900135474 92-93 22.43546554962098 26.980063656559807 27.5346084174065 23.049862376412715 94-95 22.2266461838449 26.751237865446953 27.697049518957463 23.325066431750688 96-97 22.518815249491674 26.32627032992544 27.69176584004805 23.46314858053484 98-99 22.189109014885396 26.66617132442043 27.297172264666784 23.84754739602739 100-101 23.06330715134463 25.417388516686856 27.480595079407124 24.03870925256139 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1219.0 1 842.5 2 610.0 3 786.5 4 679.0 5 662.0 6 1235.5 7 2642.5 8 4334.5 9 5036.5 10 4161.5 11 3143.5 12 3013.0 13 3193.0 14 3429.5 15 3653.0 16 3868.0 17 4093.5 18 4467.0 19 5039.0 20 5896.5 21 6883.0 22 8183.0 23 10093.5 24 12454.5 25 15356.0 26 19325.5 27 23945.5 28 27744.5 29 31625.5 30 35874.5 31 41873.5 32 50355.5 33 53751.5 34 54299.5 35 57735.5 36 62512.5 37 67506.5 38 71232.5 39 74218.0 40 77362.5 41 81333.5 42 83673.0 43 85603.0 44 88814.5 45 93914.0 46 97076.0 47 98667.5 48 100279.5 49 98225.5 50 95214.5 51 93086.0 52 92539.5 53 93650.5 54 94982.5 55 90926.0 56 83201.5 57 75612.5 58 69848.0 59 62149.5 60 50370.5 61 41719.5 62 35093.0 63 26510.5 64 18377.0 65 13214.5 66 9761.5 67 7643.5 68 5954.0 69 4552.5 70 3491.5 71 2404.0 72 1728.5 73 1298.0 74 915.5 75 609.0 76 395.5 77 281.0 78 181.5 79 113.5 80 74.5 81 47.5 82 34.0 83 20.0 84 9.0 85 7.5 86 8.5 87 5.0 88 6.0 89 4.5 90 2.5 91 2.5 92 1.5 93 1.5 94 2.5 95 1.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1445416205789687 2 0.05651577364637676 3 0.15325025321885155 4 0.12983451068505864 5 0.19166218888771247 6 0.19798588478804235 7 0.1645967704343006 8 0.07209013326376063 9 0.12051157615771514 10-11 0.10341952952425208 12-13 0.15048589472527876 14-15 0.13744101346802684 16-17 0.13458631646159222 18-19 0.13230978593747347 20-21 0.1340442853844211 22-23 0.19392065170925887 24-25 0.2126568592768077 26-27 0.1480828902831534 28-29 0.1279735373201044 30-31 0.1015404884567255 32-33 0.12501043409823553 34-35 0.09474703228951398 36-37 0.08974227867696719 38-39 0.05315518096791574 40-41 0.06177347509493675 42-43 0.06500759385539116 44-45 0.040561992274973084 46-47 0.0518001032749879 48-49 0.043488960091697206 50-51 0.05431151393254748 52-53 0.028077209797464666 54-55 0.0505534317974943 56-57 0.02825788682318838 58-59 0.05172783246469843 60-61 0.039206914582045255 62-63 0.05248667597273801 64-65 0.03989348727979536 66-67 0.03944179471548608 68-69 0.06395966710619365 70-71 0.054185040014540886 72-73 0.05207111881357347 74-75 0.04426587130230916 76-77 0.022367815784595405 78-79 0.023885502800674578 80-81 0.027354501694569825 82-83 0.023361539426075814 84-85 0.021139212009674172 86-87 0.024915361847299727 88-89 0.06544121871712807 90-91 0.10927346515770033 92-93 0.08014832861103813 94-95 0.07678773593257711 96-97 0.06959679030877343 98-99 0.05797925755473882 100-101 0.13142446851142728 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2767369.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.229044814776046 #Duplication Level Percentage of deduplicated Percentage of total 1 72.64991140815357 36.49135655909656 2 14.433765555653238 14.49988513881755 3 5.335017591517546 8.039185130758602 4 2.569173845628393 5.161885929160762 5 1.4135550904815037 3.550076099397513 6 0.8881020447448682 2.6765110443350544 7 0.5683480493173432 1.9983305747685964 8 0.3680221265293383 1.4788319909017051 9 0.28282820219038163 1.2785571398432898 >10 1.3476588227019164 12.155110443315069 >50 0.0894131709153126 3.0922399885565617 >100 0.04780409950765315 4.472001702800736 >500 0.0030921312847382106 1.131378025060417 >1k 0.0030921312846837654 2.8070080081236704 >5k 7.191002987636663E-5 0.2571362947452507 >10k+ 1.4382005975273327E-4 0.9105059303186234 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14044 0.507485629852759 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11164 0.40341566303590165 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7119 0.25724794922541955 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3655 0.13207490580403264 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3605 0.13026813554679553 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3357 0.12130655507089946 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3232 0.11678962942780671 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3072 0.11100796460464794 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 7.227081028948434E-5 0.0 0.0 0.0 0.0 3 7.227081028948434E-5 0.0 0.0 0.0 0.0 4 7.227081028948434E-5 0.0 0.0 0.0 0.0 5 7.227081028948434E-5 0.0 0.0 0.0 0.0 6 1.0840621543422652E-4 0.0 0.0 0.0 0.0 7 2.529478360131952E-4 0.0 0.0 0.0 0.0 8 2.529478360131952E-4 0.0 0.0 0.0 0.0 9 2.529478360131952E-4 0.0 0.0 0.0 0.0 10-11 2.8908324115793735E-4 0.0 0.0 0.0 0.0 12-13 3.2521864630267956E-4 0.0 0.0 1.8067702572371085E-5 0.0 14-15 4.878279694540193E-4 0.0 0.0 3.613540514474217E-5 0.0 16-17 5.600987797435037E-4 0.0 0.0 3.613540514474217E-5 0.0 18-19 6.865726977501013E-4 0.0 0.0 3.613540514474217E-5 0.0 20-21 7.949789131843277E-4 0.0 0.0 3.613540514474217E-5 1.8067702572371085E-5 22-23 9.033851286185543E-4 0.0 0.0 2.1681243086845301E-4 3.613540514474217E-5 24-25 9.756559389080386E-4 0.0 0.0 5.781664823158747E-4 3.613540514474217E-5 26-27 9.756559389080386E-4 0.0 0.0 0.001264739180065976 3.613540514474217E-5 28-29 0.001264739180065976 0.0 0.0 0.004625331858526998 3.613540514474217E-5 30-31 0.0014092808006449446 0.0 0.0 0.015068463945357486 3.613540514474217E-5 32-33 0.0014454162057896869 0.0 0.0 0.03606313433445269 3.613540514474217E-5 34-35 0.0016260932315133978 0.0 0.0 0.06601938519944395 3.613540514474217E-5 36-37 0.001879041067526593 0.0 0.0 0.11666315550980011 3.613540514474217E-5 38-39 0.0020235826881055616 0.0 0.0 0.19310760509350217 5.4203107717113254E-5 40-41 0.0020597180932503036 0.0 0.0 0.2730029858685271 7.227081028948434E-5 42-43 0.0022223274164016437 0.0 0.0 0.35714427674805926 7.227081028948434E-5 44-45 0.0024030044421253544 0.0 0.0 0.4561914222497976 7.227081028948434E-5 46-47 0.002692087683283292 0.0 0.0 0.5706141826406237 7.227081028948434E-5 48-49 0.002782426196145147 0.0 0.0 0.6890479730025161 7.227081028948434E-5 50-51 0.002782426196145147 3.613540514474217E-5 0.0 0.8158471096554164 7.227081028948434E-5 52-53 0.002800493898717518 3.613540514474217E-5 0.0 0.9383099976909477 7.227081028948434E-5 54-55 0.002818561601289889 3.613540514474217E-5 0.0 1.0699151432280987 7.227081028948434E-5 56-57 0.0028908324115793737 3.613540514474217E-5 0.0 1.2211237460562723 7.227081028948434E-5 58-59 0.0028908324115793737 3.613540514474217E-5 0.0 1.3792342112670917 7.227081028948434E-5 60-61 0.0029450355192964872 7.227081028948434E-5 0.0 1.5488176676113665 7.227081028948434E-5 62-63 0.0029631032218688583 7.227081028948434E-5 0.0 1.7116438031935748 1.0840621543422652E-4 64-65 0.0029992386270136003 7.227081028948434E-5 0.0 1.8830701652002317 1.0840621543422652E-4 66-67 0.0030353740321583424 7.227081028948434E-5 0.0 2.0805501543162475 1.0840621543422652E-4 68-69 0.003143780247592569 7.227081028948434E-5 0.0 2.2951763931734437 1.0840621543422652E-4 70-71 0.003143780247592569 7.227081028948434E-5 0.0 2.5153132813152133 1.8067702572371086E-4 72-73 0.0031979833553096824 7.227081028948434E-5 0.0 2.740527193879819 2.710155385855663E-4 74-75 0.0032521864630267955 7.227081028948434E-5 0.0 2.9662108667113056 2.8908324115793735E-4 76-77 0.0032521864630267955 7.227081028948434E-5 0.0 3.2091853309045524 2.8908324115793735E-4 78-79 0.0032521864630267955 7.227081028948434E-5 0.0 3.4774545786991182 2.8908324115793735E-4 80-81 0.003360592678461022 7.227081028948434E-5 0.0 3.7574859008682977 2.8908324115793735E-4 82-83 0.003360592678461022 7.227081028948434E-5 0.0 4.048068761339742 3.613540514474217E-4 84-85 0.0034147957861781356 7.227081028948434E-5 0.0 4.339862157883535 3.613540514474217E-4 86-87 0.0034689988938952487 7.227081028948434E-5 0.0 4.645639956218343 3.613540514474217E-4 88-89 0.0034689988938952487 7.227081028948434E-5 0.0 4.960812959890784 3.613540514474217E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6445 0.0 51.858795 1 GTATCAA 11785 0.0 42.581337 1 ATCAACG 14725 0.0 33.750576 3 TATCAAC 14840 0.0 33.617218 2 TCAACGC 14920 0.0 33.34134 4 CAACGCA 15045 0.0 33.001102 5 AACGCAG 15615 0.0 31.850443 6 TGGTATC 2810 0.0 28.09454 2 ACGCAGA 17555 0.0 27.919329 7 CGCAGAG 17580 0.0 27.714363 8 GTGGTAT 3035 0.0 26.668493 1 GCAGAGT 19145 0.0 24.902647 9 TAAGGTG 1785 0.0 23.445776 5 GAGTACT 11910 0.0 22.909096 12-13 AGGTAAG 1620 0.0 22.310986 2 GGTAAGG 1690 0.0 22.23108 3 GTACATG 10515 0.0 22.096262 1 TACATGG 10200 0.0 21.820559 2 CAGAGTA 18085 0.0 21.696352 10-11 AGAGTAC 17280 0.0 21.180449 10-11 >>END_MODULE