FastQCFastQC Report
Thu 26 May 2016
SRR938108_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938108_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2767369
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135610.49003222916784855No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113200.4090527862384814No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74120.267835622932829No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA36780.13290602012236172No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA36290.13113538527026936No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA36040.1302320001416508No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA33750.12195699236350482No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC33340.12047544075257041No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67150.053.2056161
GTATCAA117800.041.46041
TATCAAC148100.032.8448872
ATCAACG149500.032.3419843
TCAACGC152250.031.785564
CAACGCA152800.031.671155
AACGCAG158250.030.6404446
ACGCAGA179500.026.9327457
CGCAGAG179300.026.8038718
TGGTATC29450.025.969492
GTGGTAT31550.025.4491351
GCAGAGT196250.024.1500599
GAGTACT125350.023.35767412-13
GTACATG105050.022.3869421
TACATGG102350.022.1387652
CAGAGTA187600.021.6191210-11
GTACTTT134950.021.57289314-15
AGAGTAC177550.021.11759410-11
ACATGGG103600.021.043263
AGTACTT133600.020.5644812-13
Produced by FastQC (version 0.11.5)