##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938108_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2767369 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.55478904331154 34.0 31.0 34.0 31.0 34.0 2 32.7674596340423 34.0 31.0 34.0 31.0 34.0 3 32.82023069565353 34.0 31.0 34.0 31.0 34.0 4 36.26625758979016 37.0 37.0 37.0 35.0 37.0 5 36.243773056646944 37.0 37.0 37.0 35.0 37.0 6 36.27120777894093 37.0 37.0 37.0 35.0 37.0 7 36.23215841472532 37.0 37.0 37.0 35.0 37.0 8 36.215721864341184 37.0 37.0 37.0 35.0 37.0 9 37.91455385964069 39.0 38.0 39.0 35.0 39.0 10-11 37.89992931914753 39.0 38.0 39.0 35.0 39.0 12-13 37.82449142127414 39.0 38.0 39.0 35.0 39.0 14-15 39.12310429147685 40.0 38.5 41.0 36.0 41.0 16-17 39.018941818022824 40.0 38.0 41.0 36.0 41.0 18-19 39.2666975383478 40.5 39.0 41.0 36.0 41.0 20-21 39.25214255850955 41.0 39.0 41.0 36.0 41.0 22-23 39.16417525093328 40.5 39.0 41.0 36.0 41.0 24-25 39.07132731486116 40.5 39.0 41.0 35.0 41.0 26-27 38.90682413512618 40.0 38.0 41.0 35.0 41.0 28-29 38.79981870867239 40.0 38.0 41.0 35.0 41.0 30-31 38.55345275602928 40.0 38.0 41.0 34.5 41.0 32-33 38.336183031608726 40.0 38.0 41.0 34.0 41.0 34-35 38.1801143974656 40.0 38.0 41.0 34.0 41.0 36-37 38.16167160938783 40.0 38.0 41.0 34.0 41.0 38-39 38.01339449130202 40.0 38.0 41.0 33.0 41.0 40-41 37.84041755906061 40.0 37.5 41.0 33.0 41.0 42-43 37.605472923921596 40.0 37.0 41.0 33.0 41.0 44-45 37.32986565940429 40.0 37.0 41.0 32.5 41.0 46-47 36.936766654537216 40.0 36.5 41.0 31.0 41.0 48-49 36.53243062273228 39.5 36.0 41.0 30.5 41.0 50-51 36.16198689079772 39.0 35.0 41.0 30.0 41.0 52-53 35.67525346276554 39.0 35.0 40.0 28.0 41.0 54-55 35.382422799417064 38.5 35.0 40.0 27.5 41.0 56-57 35.05670566520041 38.0 34.5 40.0 27.0 41.0 58-59 34.641281845680865 38.0 34.0 40.0 26.0 41.0 60-61 34.19439420619368 37.0 34.0 40.0 25.5 41.0 62-63 33.75824546708444 37.0 33.0 39.5 23.5 41.0 64-65 33.291701251260676 36.0 33.0 39.0 22.5 40.5 66-67 32.772663313060164 35.5 32.0 39.0 21.5 40.0 68-69 32.23190745433659 35.0 31.0 38.0 19.5 40.0 70-71 31.66085549126264 35.0 31.0 37.5 17.5 40.0 72-73 31.11756527589924 35.0 30.5 37.0 13.5 39.0 74-75 30.549493580364597 34.0 30.0 36.5 8.0 39.0 76-77 29.189177518429958 32.5 28.5 35.0 7.5 37.5 78-79 29.880439507705695 34.0 29.5 35.0 7.0 37.5 80-81 29.59097576073158 34.0 29.0 35.0 2.0 37.0 82-83 29.063429741389747 34.0 29.0 35.0 2.0 37.0 84-85 28.504022773977738 34.0 28.5 35.0 2.0 36.0 86-87 27.91140231028099 33.0 26.5 35.0 2.0 36.0 88-89 27.206254749547313 33.0 26.0 35.0 2.0 35.0 90-91 26.70459649580522 32.5 25.0 35.0 2.0 35.0 92-93 25.943556858517965 32.0 23.5 34.0 2.0 35.0 94-95 25.170075620562343 32.0 19.5 34.0 2.0 35.0 96-97 24.18028441454681 31.0 9.5 34.0 2.0 35.0 98-99 22.794146172772766 31.0 2.0 34.0 2.0 35.0 100-101 18.359101189613675 24.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.11995471381124645 1101 2 0.08759305967587494 1101 3 0.0751370424553528 1101 4 0.06033311830830712 1101 5 -0.031038319610217968 1101 6 -0.011911871597966694 1101 7 1.5657833400695154E-4 1101 8 0.031957619400778015 1101 9 0.007296128741813845 1101 10-11 0.044763276737704416 1101 12-13 0.10469563385124303 1101 14-15 0.11009978357519401 1101 16-17 0.21168599836249058 1101 18-19 0.18145702728685364 1101 20-21 0.08762676813253734 1101 22-23 0.15774376488444375 1101 24-25 0.14423739025030358 1101 26-27 0.17322665029762874 1101 28-29 0.14981492221819792 1101 30-31 0.19149952929465996 1101 32-33 0.18244673189963123 1101 34-35 0.13545608898287753 1101 36-37 0.20412111862705018 1101 38-39 0.13043867238468465 1101 40-41 0.13700818312533158 1101 42-43 0.2220156580543744 1101 44-45 0.21587704334626778 1101 46-47 0.3007019980555654 1101 48-49 0.3345448993631095 1101 50-51 0.27466286987628763 1101 52-53 0.05354066904195065 1101 54-55 -0.10555277023215837 1101 56-57 0.0184456629082419 1101 58-59 -4.25932106274729E-4 1101 60-61 0.09116978501413797 1101 62-63 0.10866937795142917 1101 64-65 0.04169434230302471 1101 66-67 -0.15874728254744497 1101 68-69 0.0014762297536918823 1101 70-71 0.014548026005630987 1101 72-73 -0.017532801028032452 1101 74-75 -0.23515387374035868 1101 76-77 -0.437991943399652 1101 78-79 -0.4185909267620396 1101 80-81 -0.3744258714178734 1101 82-83 -0.3604171070972768 1101 84-85 -0.5543952405011865 1101 86-87 -0.5847418951702714 1101 88-89 -0.6399146077035631 1101 90-91 -0.7733735648828066 1101 92-93 -1.0239352138374294 1101 94-95 -1.0139863222351408 1101 96-97 -1.0182687954941088 1101 98-99 -0.765031714883488 1101 100-101 -1.0465962043906352 1102 1 0.15977651935283887 1102 2 0.12167133896358706 1102 3 0.10425730431514069 1102 4 0.08616831665192848 1102 5 0.06682172437404432 1102 6 0.08462211920660678 1102 7 0.12552107100712107 1102 8 0.09822253727859476 1102 9 0.21305041297467397 1102 10-11 0.17820480712193643 1102 12-13 0.1604239931183642 1102 14-15 0.1831619805661333 1102 16-17 0.175974975313963 1102 18-19 0.16102101168954164 1102 20-21 0.20577380325116224 1102 22-23 0.1930788759297144 1102 24-25 0.11374504516960826 1102 26-27 0.16030861022503728 1102 28-29 0.044203842558005135 1102 30-31 0.15114352837053247 1102 32-33 0.09507986188952344 1102 34-35 0.14230592346872584 1102 36-37 0.25486588822111145 1102 38-39 0.14920975846439433 1102 40-41 0.10471793285240949 1102 42-43 0.20372488928214239 1102 44-45 0.04176929425054965 1102 46-47 -0.007843629002344699 1102 48-49 -0.025752867386266587 1102 50-51 0.10752232305350873 1102 52-53 0.08625691764649446 1102 54-55 0.2052327209577598 1102 56-57 0.07679191463436297 1102 58-59 0.07059912693033965 1102 60-61 -0.07134244998245265 1102 62-63 0.07892744599476487 1102 64-65 0.09864179222110891 1102 66-67 -0.06612425800337718 1102 68-69 0.034419365898564536 1102 70-71 -0.09117610061401749 1102 72-73 0.0021676157113859063 1102 74-75 -0.10134692479137541 1102 76-77 -0.15448321973686063 1102 78-79 -0.28622925056309967 1102 80-81 -0.15665060383519247 1102 82-83 -0.16681925093322292 1102 84-85 -0.36889690798792785 1102 86-87 -0.4037483340852326 1102 88-89 -0.4802031508086664 1102 90-91 -0.8943316951813323 1102 92-93 -0.8385905793353565 1102 94-95 -0.7990383749809808 1102 96-97 -0.8134580389260151 1102 98-99 -0.576215427656031 1102 100-101 -1.0740876905966132 1103 1 0.16156205385836842 1103 2 0.17779377787146444 1103 3 0.13354836124496927 1103 4 0.07712318773614157 1103 5 0.1050365057176279 1103 6 0.0181872068251252 1103 7 0.04893654484987309 1103 8 0.13638994125552983 1103 9 0.1391377728906562 1103 10-11 0.11165342538078704 1103 12-13 0.13718736883470228 1103 14-15 0.18629382190722765 1103 16-17 0.23171888880289515 1103 18-19 0.233497031832826 1103 20-21 0.18013524518131874 1103 22-23 0.20566941111628978 1103 24-25 0.16774981449118087 1103 26-27 0.1734617516405521 1103 28-29 0.291197847347064 1103 30-31 0.27788341280923845 1103 32-33 0.35904532773734843 1103 34-35 0.37349365431001047 1103 36-37 0.22565774168291597 1103 38-39 0.2436520972480949 1103 40-41 0.20416006436013845 1103 42-43 0.24062445518701736 1103 44-45 0.34440801616712946 1103 46-47 0.3675484680580894 1103 48-49 0.49421077048483397 1103 50-51 0.5243187839642189 1103 52-53 0.3584127457535118 1103 54-55 0.17555524238121478 1103 56-57 0.36524456983965337 1103 58-59 0.43704381089369093 1103 60-61 0.33402718230995987 1103 62-63 0.3703012119624205 1103 64-65 0.23135073134316286 1103 66-67 0.20625069042437616 1103 68-69 0.3683829169111519 1103 70-71 0.005452012720542854 1103 72-73 -0.0734802204260987 1103 74-75 -0.027603066576041613 1103 76-77 0.1292183739193078 1103 78-79 0.004162422075090433 1103 80-81 0.09477466527811984 1103 82-83 0.14924503225709174 1103 84-85 0.025265016006930097 1103 86-87 -0.3637026149264173 1103 88-89 -0.6822947628433091 1103 90-91 -0.700412678893386 1103 92-93 -0.8368835135933388 1103 94-95 -0.7408841379638602 1103 96-97 -0.881142035838586 1103 98-99 -1.1161979769926518 1103 100-101 -1.6228523257612792 1104 1 0.12351461145269838 1104 2 0.1428596170243992 1104 3 0.19724064519388662 1104 4 0.09304419177632894 1104 5 0.01216811604390955 1104 6 0.05011189278697259 1104 7 0.10526498548991015 1104 8 0.08851761060279983 1104 9 0.15150087209860175 1104 10-11 0.14762105974612183 1104 12-13 0.1922632395705861 1104 14-15 0.22074385782617867 1104 16-17 0.19481653505547314 1104 18-19 0.14382368295603953 1104 20-21 0.2534388328501862 1104 22-23 0.11026464776794853 1104 24-25 0.21151351375682026 1104 26-27 0.2549008428452808 1104 28-29 0.11897982026179221 1104 30-31 0.06972806658182407 1104 32-33 0.037421938348416006 1104 34-35 0.034535009786317517 1104 36-37 -0.07257056318166377 1104 38-39 0.057019640851493136 1104 40-41 0.07435624978641187 1104 42-43 0.09195538534993375 1104 44-45 0.059307121592446777 1104 46-47 0.14999586195940395 1104 48-49 0.07657723192662047 1104 50-51 0.1236594737917116 1104 52-53 0.08955254302735227 1104 54-55 0.04599303094997964 1104 56-57 0.16801832089839053 1104 58-59 0.2366946346409904 1104 60-61 0.04477999197996496 1104 62-63 0.04566651040200753 1104 64-65 0.1355670277047878 1104 66-67 -0.03385138919993125 1104 68-69 0.0060930335604538755 1104 70-71 -0.03238827565990832 1104 72-73 -0.13575963124514345 1104 74-75 -0.07941270167261649 1104 76-77 -0.19969169275580967 1104 78-79 -0.3380638360526049 1104 80-81 -0.21565046438835722 1104 82-83 -0.21066143149968042 1104 84-85 -0.2970220930415195 1104 86-87 -0.42173697585394976 1104 88-89 -0.6218204857695184 1104 90-91 -0.6641937956327162 1104 92-93 -0.6493645665788357 1104 94-95 -0.8140260444423184 1104 96-97 -0.8646928120001647 1104 98-99 -0.8402522798251937 1104 100-101 -1.3912265662730725 1105 1 0.11290281806657276 1105 2 0.056422871581986556 1105 3 0.07115063009005951 1105 4 0.013723165759095934 1105 5 0.008109668606131493 1105 6 0.005142006278632039 1105 7 0.040288249672457255 1105 8 0.08418623384240931 1105 9 0.09961532741482415 1105 10-11 0.026274640291994444 1105 12-13 0.072152317848861 1105 14-15 0.049931968422235684 1105 16-17 -0.008321978314150158 1105 18-19 0.010931730227866865 1105 20-21 0.06768210732987257 1105 22-23 0.015438730028613179 1105 24-25 0.030333497166658674 1105 26-27 -0.03285640825230729 1105 28-29 0.024716638166651705 1105 30-31 -0.061487992904012856 1105 32-33 0.06637461118698695 1105 34-35 0.15260193136086286 1105 36-37 0.05590403425483004 1105 38-39 0.0865443208186818 1105 40-41 0.09811747081773348 1105 42-43 0.08159645721465125 1105 44-45 0.0805319397337314 1105 46-47 -0.09645649144756163 1105 48-49 0.13401798931418085 1105 50-51 0.10390172319291224 1105 52-53 -0.0480845988869163 1105 54-55 0.20875292586929817 1105 56-57 0.28712516983085123 1105 58-59 0.2507299236490965 1105 60-61 0.034007106552046196 1105 62-63 -0.05066826899315657 1105 64-65 0.006058659755488804 1105 66-67 -0.012167342845984308 1105 68-69 0.028970566233020634 1105 70-71 -0.13429876643145278 1105 72-73 -0.20267925008282717 1105 74-75 -0.21331647757156702 1105 76-77 -0.22856831044875747 1105 78-79 -0.23005422963580813 1105 80-81 -0.20728584703845243 1105 82-83 -0.14370436455695668 1105 84-85 -0.3888418043912374 1105 86-87 -0.502444740693182 1105 88-89 -0.5950273444660112 1105 90-91 -0.7588641987076024 1105 92-93 -0.7320081883915108 1105 94-95 -0.6427639085762458 1105 96-97 -0.6955311743894619 1105 98-99 -0.8193285676271351 1105 100-101 -1.5250997028133568 1106 1 0.09675494485036751 1106 2 0.15215374738594534 1106 3 0.14569245804926823 1106 4 0.048712341992271035 1106 5 0.07277321145409843 1106 6 0.02286320321324098 1106 7 0.0695014963949987 1106 8 0.014007845831407906 1106 9 -0.0016812159058829934 1106 10-11 0.06825553139689333 1106 12-13 0.018988107260241804 1106 14-15 0.021751283178858216 1106 16-17 0.10045110653918954 1106 18-19 0.17338786302171627 1106 20-21 0.19063765032101543 1106 22-23 0.22429962289369598 1106 24-25 0.2511427563797213 1106 26-27 0.2319333227812308 1106 28-29 0.15041341186137203 1106 30-31 0.07312515989013946 1106 32-33 0.27291882593746664 1106 34-35 0.22350903135213684 1106 36-37 0.2037143422987171 1106 38-39 0.22477329814432068 1106 40-41 0.21210511509782748 1106 42-43 0.16801026304506905 1106 44-45 0.19080684063401776 1106 46-47 0.27390637550958985 1106 48-49 0.20832011550144358 1106 50-51 0.25245546310610933 1106 52-53 0.17935806210954297 1106 54-55 0.0963864836835242 1106 56-57 0.06691717934827324 1106 58-59 0.12565076550898624 1106 60-61 -0.1122325118588492 1106 62-63 0.11055332994936151 1106 64-65 0.10760009554586958 1106 66-67 -0.15509366918158918 1106 68-69 -0.15945788108363956 1106 70-71 -0.5476475830220977 1106 72-73 -0.4493983804999573 1106 74-75 -0.3883721381203884 1106 76-77 -0.4906319955063516 1106 78-79 -0.45835730688284215 1106 80-81 -0.3544883011383497 1106 82-83 -0.4932805789516763 1106 84-85 -0.4177589489248952 1106 86-87 -0.5024882805754807 1106 88-89 -0.5093867201753746 1106 90-91 -0.7794561613692856 1106 92-93 -0.6861885826459222 1106 94-95 -0.9905536707539717 1106 96-97 -1.1523759460320733 1106 98-99 -1.0306105723458678 1106 100-101 -1.3879472916877837 1107 1 0.06108978228264306 1107 2 0.053412214183971685 1107 3 0.04409045976045434 1107 4 0.008890735318672682 1107 5 -0.044549318959703044 1107 6 0.031781322347384844 1107 7 0.0720869843837022 1107 8 0.04334909775501217 1107 9 -5.661169626520746E-4 1107 10-11 0.07831983384188135 1107 12-13 0.06229953197496485 1107 14-15 0.0789475744903001 1107 16-17 -0.04532719328093293 1107 18-19 0.023660160675497366 1107 20-21 0.04258315900262488 1107 22-23 0.010394586621330859 1107 24-25 0.10311273872633109 1107 26-27 0.18358721508557352 1107 28-29 0.11103830892077582 1107 30-31 -0.03402490573163419 1107 32-33 0.06203825643810035 1107 34-35 0.09451748559686735 1107 36-37 0.042477266046518025 1107 38-39 0.07762897398809798 1107 40-41 0.0033831509821311556 1107 42-43 0.13093329350929395 1107 44-45 -6.505000097192237E-4 1107 46-47 -0.033584619517903036 1107 48-49 -0.002421277609897743 1107 50-51 0.04449415175087523 1107 52-53 0.03471870195804172 1107 54-55 0.0990817515316067 1107 56-57 0.07905182726435811 1107 58-59 0.17954402629150223 1107 60-61 -0.008755183826075097 1107 62-63 0.0271837901259957 1107 64-65 -0.16915654747183595 1107 66-67 -0.16336344717309714 1107 68-69 -0.014475467662400376 1107 70-71 -0.05059898043320388 1107 72-73 -0.2321270900640826 1107 74-75 -0.2099992767705956 1107 76-77 -0.21222886557777088 1107 78-79 -0.14922629499384854 1107 80-81 -0.01698893583612815 1107 82-83 -0.020829064587388757 1107 84-85 -0.07906002240454413 1107 86-87 -0.2948785320114453 1107 88-89 -0.4766912399408838 1107 90-91 -0.6762638671418237 1107 92-93 -0.6368983400289174 1107 94-95 -0.8605982282961264 1107 96-97 -0.8150228601068648 1107 98-99 -0.906725583380819 1107 100-101 -1.5715182047650629 1108 1 -0.03075506424001162 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2316 80-81 0.5323213314016009 2316 82-83 0.6653002722728871 2316 84-85 0.5212898374619748 2316 86-87 0.7290527770690183 2316 88-89 0.8881424247525267 2316 90-91 1.02330287814085 2316 92-93 1.1679745727306425 2316 94-95 1.4943756476350778 2316 96-97 1.7676990486826867 2316 98-99 2.227464653103848 2316 100-101 2.8703485374559143 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 10.0 4 14.0 5 38.0 6 93.0 7 238.0 8 564.0 9 1197.0 10 2111.0 11 3579.0 12 5463.0 13 8485.0 14 11298.0 15 15387.0 16 20507.0 17 28850.0 18 28722.0 19 21721.0 20 19774.0 21 20103.0 22 21451.0 23 23327.0 24 26525.0 25 30427.0 26 34378.0 27 41171.0 28 49482.0 29 60498.0 30 73690.0 31 90486.0 32 113866.0 33 148755.0 34 204182.0 35 296302.0 36 433662.0 37 566159.0 38 342940.0 39 21909.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.150914635248135 19.047968388725582 14.961219523301173 22.839897452725115 2 15.622101928277084 20.14788252378271 40.11502295327016 24.114992594670042 3 19.06924032096956 24.174664162976907 30.3005005933976 26.455594922655933 4 12.800326086131692 17.00074475123827 36.92856784083933 33.270361321790716 5 13.420333909338144 37.48212180803357 34.48259663257615 14.614947650052143 6 32.03851674329928 36.50660061951725 17.116404487236007 14.33847814994746 7 28.340456223944116 33.299101059526215 20.337620317348353 18.022822399181315 8 26.561654770289035 34.73775994455384 19.59959080267214 19.10099448248499 9 26.334796696790345 16.630272291118388 20.230406570283904 36.80452444180736 10-11 24.936428788499114 26.747914716107612 27.00651774302596 21.30913875236732 12-13 25.936089411153375 24.078879780497626 27.59471559529593 22.390315213053064 14-15 22.560146478478295 25.47247945611879 26.211466559031336 25.75590750637157 16-17 21.76241633099086 28.604728141443132 27.2088925571215 22.423962970444506 18-19 21.77167193822002 26.7641395130176 29.915869549742013 21.54831899902037 20-21 22.339539830069644 25.931543643077593 30.301994421416158 21.426922105436606 22-23 22.031864922964736 25.964589471082462 30.253301962983613 21.75024364296919 24-25 21.838937367141533 26.12273254127929 30.352792591274273 21.685537500304903 26-27 21.44988364954959 26.47949035304144 30.06395525029939 22.00667074710958 28-29 21.64359522723675 26.91554501622939 29.91167612628207 21.529183630251786 30-31 21.728526758821708 26.41115342683265 29.803403542740142 22.056916271605502 32-33 21.333096525978284 26.42237085115863 29.854475496401093 22.390057126461993 34-35 22.196334972578065 26.582416859646536 29.583452590555986 21.637795577219414 36-37 22.282086986875704 26.129632751350258 29.59605557449667 21.99222468727737 38-39 21.79250856883708 26.495657311562375 29.57339206698238 22.13844205261817 40-41 22.18405639123488 26.31830664405264 29.651002173772035 21.846634790940442 42-43 21.953778153276254 26.31549087200828 29.400139378513483 22.33059159620198 44-45 21.950000162609324 26.579921217589703 29.59887170810976 21.871206911691214 46-47 22.450780832466386 26.888555077805005 28.69906665794598 21.96159743178263 48-49 22.120333806469144 26.95220918770001 28.619886447405317 22.307570558425528 50-51 22.29826066798314 27.1945824730449 28.539929941036927 21.967226917935033 52-53 22.629122097307352 26.85624703438112 28.485227829999975 22.02940303831155 54-55 22.13133800844556 27.1267574585266 28.56093034853739 22.180974184490456 56-57 22.20020614994734 27.32775239454151 28.23591085821842 22.23613059729273 58-59 22.10574976366663 26.935173182528153 28.36679951213491 22.592277541670303 60-61 21.78846029452206 27.166192732674848 28.607906079039108 22.437440893763984 62-63 21.9218269532419 27.118557081084678 28.78162999180291 22.177985973870516 64-65 21.721589433605747 27.259917966885684 28.44756749606361 22.57092510344496 66-67 21.661757047390743 27.307945704706547 28.423117747379216 22.60717950052349 68-69 22.200050568479714 26.95932526683548 28.509073861246815 22.33155030343799 70-71 22.053960946566352 27.081542086220562 28.060028327454972 22.804468639758113 72-73 21.92182962490767 27.211937502439277 28.110856703425686 22.755376169227368 74-75 22.01122036970912 27.631380171251045 27.723883348054812 22.63351611098502 76-77 22.070935043315178 27.24564327260281 28.126385845375168 22.55703583870684 78-79 22.03935276634304 27.512328010562893 28.000785783037855 22.447533440056212 80-81 22.259064473839942 27.454123114758396 27.76467016798561 22.522142243416056 82-83 22.245603723496547 27.592075279638177 27.75148639113486 22.410834605730415 84-85 22.064153086085987 27.776454527809996 27.65410912487074 22.505283261233277 86-87 22.3490656539916 27.664163896051885 27.43127321045033 22.555497239506185 88-89 22.136277376998343 27.80660711087674 27.200013445322263 22.85710206680265 90-91 21.998025385882 27.823371978009963 27.40813553305997 22.770467103048073 92-93 22.16143615752394 27.509292605384868 27.515617793803195 22.813653443287997 94-95 21.96151428211572 27.28597607242134 27.68202236775221 23.070487277710725 96-97 22.142130196504752 26.950193642399807 27.703313677557578 23.204362483537867 98-99 21.847432403032528 27.351356782030905 27.32974541228205 23.471465402654513 100-101 22.35639223741726 26.766884904003096 27.349138238683214 23.52758461989643 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1175.0 1 803.5 2 590.0 3 791.0 4 686.0 5 660.0 6 1295.0 7 2774.0 8 4451.5 9 5109.0 10 4151.0 11 3055.0 12 2905.5 13 2998.5 14 3214.5 15 3468.0 16 3739.0 17 3989.0 18 4276.0 19 4854.0 20 5542.5 21 6416.0 22 7839.0 23 9669.5 24 11938.0 25 14847.0 26 18893.0 27 23760.5 28 27580.0 29 31216.0 30 35579.0 31 41904.5 32 51124.0 33 54665.0 34 54487.0 35 57682.5 36 62966.5 37 68053.5 38 71378.5 39 74124.0 40 77643.5 41 82057.5 42 84211.5 43 86620.0 44 90294.0 45 95942.0 46 99129.0 47 100870.0 48 102278.5 49 99790.0 50 96496.5 51 93754.0 52 93540.0 53 94400.5 54 96265.0 55 91917.5 56 83079.0 57 75205.0 58 69178.0 59 61287.5 60 48919.5 61 40168.0 62 33646.0 63 24711.0 64 16354.0 65 11684.5 66 8748.0 67 6759.5 68 5149.0 69 3893.5 70 2918.5 71 1918.5 72 1338.5 73 1072.0 74 755.0 75 480.5 76 330.0 77 201.5 78 130.0 79 77.5 80 32.5 81 18.0 82 14.0 83 8.5 84 6.0 85 4.0 86 2.5 87 1.5 88 1.5 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009214528311909253 2 0.01600798447912078 3 0.00914225750161977 4 7.227081028948434E-5 5 0.0011201975594870074 6 0.001915176472671335 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 6.685049951777301E-4 14-15 0.0 16-17 0.0023849367395529834 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0033063895707439086 26-27 0.018519395136680362 28-29 0.006034612659171943 30-31 0.002746290791000405 32-33 0.0 34-35 0.008708632639882863 36-37 7.407758054672146E-4 38-39 0.0026740199807109206 40-41 0.002150056606112159 42-43 0.0030534417347307134 44-45 0.0 46-47 7.227081028948434E-5 48-49 6.143018874606169E-4 50-51 0.0046433995610993695 52-53 0.008672497234738123 54-55 0.008455684803869669 56-57 0.01629706772027872 58-59 0.022422018892312517 60-61 0.00383035294534267 62-63 0.01926017094214758 64-65 0.020217759178483244 66-67 0.012719662610949246 68-69 0.02975750613669518 70-71 0.01597184907397604 72-73 0.005673258607724521 74-75 0.015791172048252332 76-77 0.020091285260476648 78-79 0.025367054411609004 80-81 0.011436855728310897 82-83 0.03385887462062341 84-85 0.02211486794858221 86-87 0.030173063295859715 88-89 0.02195225862543087 90-91 0.027191892371418485 92-93 0.02359641955951664 94-95 0.014255417329600787 96-97 0.014923922324778518 98-99 0.011093569379435847 100-101 0.009919168712231726 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2767369.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.88420436579047 #Duplication Level Percentage of deduplicated Percentage of total 1 70.26592673562867 33.64627995760504 2 15.553026030150999 14.894885538684182 3 5.8669630973359785 8.42804579878161 4 2.8196074484110247 5.40058637164074 5 1.5364127000862204 3.6784949860562257 6 0.9530222734587656 2.7380827984469867 7 0.6055792312949291 2.029837576970327 8 0.4440255296118732 1.7009447362850625 9 0.3132279083193186 1.3498802257528202 >10 1.481066828717736 12.68824085630142 >50 0.10046071863957437 3.3233095606952388 >100 0.05336728368726687 4.756708707750978 >500 0.0037702137410912774 1.284858238142095 >1k 0.0033177880921246273 2.9138497871430817 >5k 7.540427482101425E-5 0.267623135057862 >10k+ 1.508085496420285E-4 0.8983717246862741 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13561 0.49003222916784855 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11320 0.4090527862384814 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7412 0.267835622932829 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3678 0.13290602012236172 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3629 0.13113538527026936 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3604 0.1302320001416508 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3375 0.12195699236350482 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3334 0.12047544075257041 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 3.613540514474217E-5 0.0 0.0 0.0 0.0 6 7.227081028948434E-5 0.0 0.0 0.0 0.0 7 1.0840621543422652E-4 0.0 0.0 0.0 0.0 8 1.0840621543422652E-4 0.0 0.0 0.0 0.0 9 1.0840621543422652E-4 0.0 0.0 0.0 0.0 10-11 1.0840621543422652E-4 0.0 0.0 0.0 0.0 12-13 3.252186463026795E-4 0.0 0.0 1.8067702572371085E-5 0.0 14-15 3.974894565921639E-4 0.0 0.0 3.613540514474217E-5 3.613540514474217E-5 16-17 4.1555715916453495E-4 0.0 0.0 3.613540514474217E-5 3.613540514474217E-5 18-19 5.058956720263904E-4 0.0 0.0 3.613540514474217E-5 3.613540514474217E-5 20-21 5.781664823158747E-4 0.0 0.0 3.613540514474217E-5 3.613540514474217E-5 22-23 5.781664823158747E-4 0.0 0.0 2.1681243086845301E-4 5.4203107717113254E-5 24-25 8.672497234738122E-4 0.0 0.0 5.781664823158747E-4 7.227081028948434E-5 26-27 8.672497234738122E-4 0.0 0.0 0.001264739180065976 7.227081028948434E-5 28-29 0.0011924683697764917 0.0 0.0 0.0046433995610993695 7.227081028948434E-5 30-31 0.0013008745852107183 0.0 0.0 0.0151226670530746 7.227081028948434E-5 32-33 0.0013189422877830893 0.0 0.0 0.036171540549886916 7.227081028948434E-5 34-35 0.0014273485032173158 0.0 0.0 0.0662361976303124 7.227081028948434E-5 36-37 0.0014815516109344291 0.0 0.0 0.11724132199211598 7.227081028948434E-5 38-39 0.0017344994469476243 0.0 0.0 0.19422780265298917 1.0840621543422652E-4 40-41 0.0017887025546647374 0.0 0.0 0.27448453747946155 1.0840621543422652E-4 42-43 0.0020055149855331906 0.0 0.0 0.35905945322073063 1.0840621543422652E-4 44-45 0.0023488013344082413 0.0 0.0 0.4588293068253637 1.264739180065976E-4 46-47 0.002619816872993807 0.0 0.0 0.5740109107242294 1.4454162057896868E-4 48-49 0.0026740199807109206 0.0 0.0 0.6929686644607207 1.8067702572371086E-4 50-51 0.0026740199807109206 0.0 0.0 0.8209060663756803 1.8067702572371086E-4 52-53 0.002692087683283292 0.0 0.0 0.9444891519706985 1.8067702572371086E-4 54-55 0.002746290791000405 0.0 0.0 1.077431307498205 1.8067702572371086E-4 56-57 0.0028366293038622602 0.0 0.0 1.22948909234728 1.8067702572371086E-4 58-59 0.0028546970064346317 0.0 0.0 1.3887920259278759 1.8067702572371086E-4 60-61 0.0028727647090070027 0.0 0.0 1.5599293046933749 1.8067702572371086E-4 62-63 0.0028908324115793737 0.0 0.0 1.7239840440505043 1.8067702572371086E-4 64-65 0.0028908324115793737 0.0 0.0 1.895699489298319 1.8067702572371086E-4 66-67 0.0029631032218688583 0.0 0.0 2.0951488579947233 1.8067702572371086E-4 68-69 0.0029631032218688583 0.0 0.0 2.313352501961249 1.8067702572371086E-4 70-71 0.0029811709244412293 0.0 0.0 2.5369041858891963 1.8067702572371086E-4 72-73 0.0030353740321583424 0.0 0.0 2.7659484513991446 1.8067702572371086E-4 74-75 0.0030715094373030844 0.0 0.0 2.9971247058126327 1.8067702572371086E-4 76-77 0.0030715094373030844 0.0 0.0 3.2451761944287156 2.1681243086845304E-4 78-79 0.0030715094373030844 0.0 0.0 3.519317445559302 2.3488013344082412E-4 80-81 0.0032702541655991665 0.0 0.0 3.806684254972864 2.529478360131952E-4 82-83 0.003360592678461022 0.0 0.0 4.104692941201552 2.529478360131952E-4 84-85 0.003378660381033393 0.0 0.0 4.404942022549216 2.529478360131952E-4 86-87 0.003414795786178135 0.0 0.0 4.719717536765065 2.529478360131952E-4 88-89 0.0034509311913228776 0.0 0.0 5.046056380627231 2.529478360131952E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6715 0.0 53.205616 1 GTATCAA 11780 0.0 41.4604 1 TATCAAC 14810 0.0 32.844887 2 ATCAACG 14950 0.0 32.341984 3 TCAACGC 15225 0.0 31.78556 4 CAACGCA 15280 0.0 31.67115 5 AACGCAG 15825 0.0 30.640444 6 ACGCAGA 17950 0.0 26.932745 7 CGCAGAG 17930 0.0 26.803871 8 TGGTATC 2945 0.0 25.96949 2 GTGGTAT 3155 0.0 25.449135 1 GCAGAGT 19625 0.0 24.150059 9 GAGTACT 12535 0.0 23.357674 12-13 GTACATG 10505 0.0 22.386942 1 TACATGG 10235 0.0 22.138765 2 CAGAGTA 18760 0.0 21.61912 10-11 GTACTTT 13495 0.0 21.572893 14-15 AGAGTAC 17755 0.0 21.117594 10-11 ACATGGG 10360 0.0 21.04326 3 AGTACTT 13360 0.0 20.56448 12-13 >>END_MODULE