FastQCFastQC Report
Thu 26 May 2016
SRR938107_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938107_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2005731
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127800.6371741773946756No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97880.48800163132543695No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65160.3248690876293979No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21860.10898769575780601No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61550.046.655471
GTATCAA103950.041.3460921
TATCAAC131900.032.355492
ATCAACG133400.031.9916743
CAACGCA136400.031.2532025
TCAACGC137500.031.0723044
AACGCAG140100.030.5913166
ACGCAGA158000.026.8803147
CGCAGAG158350.026.5508948
GTACATG93100.026.1309191
TACATGG94750.025.1289862
GCAGAGT164100.024.9528489
GAGTACT95800.024.09862112-13
ACATGGG96650.023.8482423
GTACTTT101850.022.6210614-15
AGTACTT100350.021.63317512-13
AGAGTAC150600.021.13375510-11
CAGAGTA161000.021.02257210-11
CATGGGA48000.020.4948884
ACTTTTT121150.019.17278516-17
Produced by FastQC (version 0.11.5)