FastQCFastQC Report
Thu 26 May 2016
SRR938107_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938107_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2005731
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120050.5985348982490672No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97900.4881013455941999No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64440.321279373953935No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21860.10898769575780601No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68250.041.6794171
GTATCAA110450.038.503031
TATCAAC138000.030.8508432
ATCAACG138950.030.5661073
TCAACGC143500.029.6963674
CAACGCA143850.029.6211555
AACGCAG147350.028.912316
ACGCAGA163100.025.9456317
CGCAGAG163100.025.8000348
GTACATG98000.025.19211
TACATGG99600.024.5008472
GCAGAGT172700.024.0633589
ACATGGG100000.023.1144453
GAGTACT93350.022.61506812-13
AGTACTT96900.021.61500412-13
GTACTTT98850.021.26067714-15
GTATAGG3401.8189894E-1220.986281
AGAGTAC154900.020.91093610-11
CATGGGA49900.019.9201644
CAGAGTA168950.019.8888110-11
Produced by FastQC (version 0.11.5)