FastQCFastQC Report
Thu 26 May 2016
SRR938106_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938106_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1966133
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124410.6327649248550328No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96390.4902516767685604No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63180.3213414351928379No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21600.10986031972404714No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66150.040.845451
GTATCAA107200.039.0982061
TATCAAC133550.031.178512
ATCAACG136300.030.5502263
TCAACGC139800.029.7513774
CAACGCA140550.029.626445
AACGCAG144150.029.0484686
GCGTTAG856.224802E-428.0807171
GCGTACA1405.774509E-727.1617092
ACGCAGA161350.025.6776147
CGCAGAG161600.025.4026818
GTACATG96600.024.8569581
TACATGG97900.024.1791922
ATTCGCG2100.023.74351780-81
GCAGAGT169700.023.600429
GAGTACT93700.023.16924712-13
ACATGGG99750.022.15883
AGTACTT96150.021.86247312-13
GTACTTT99650.021.62063614-15
AGAGTAC151500.020.6477610-11
Produced by FastQC (version 0.11.5)