FastQCFastQC Report
Thu 26 May 2016
SRR938106_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938106_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1966133
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117620.5982301299047419No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96060.48857325521722084No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65360.33242918968350565No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22320.1135223303815154No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61250.047.6168481
GTATCAA100400.037.9909551
TATCAAC126250.030.0240922
ATCAACG128400.029.3718813
CAACGCA131650.028.7535615
TCAACGC132000.028.7133034
AACGCAG135100.028.0896036
GTACATG91800.025.4577941
TACATGG93700.024.5360222
ACGCAGA153500.024.505927
CGCAGAG153800.024.303718
ACATGGG93350.023.4056023
GTAAGGT6150.023.1686294
GCAGAGT162450.022.6587649
GAGTACT93650.022.41654612-13
GTACTTT99050.020.9786614-15
AGTACTT96250.020.97212412-13
CATGGGG45650.020.9126684
TAAGGTG8300.020.600545
CAACGCG1656.3300606E-520.1496879
Produced by FastQC (version 0.11.5)