##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938106_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1966133 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.51164290513409 34.0 31.0 34.0 31.0 34.0 2 32.73630013839349 34.0 31.0 34.0 31.0 34.0 3 32.69510099265919 34.0 31.0 34.0 31.0 34.0 4 36.21900298708175 37.0 35.0 37.0 35.0 37.0 5 36.18971249656051 37.0 35.0 37.0 35.0 37.0 6 36.25175916380021 37.0 37.0 37.0 35.0 37.0 7 36.21102132968624 37.0 37.0 37.0 35.0 37.0 8 36.20803170487449 37.0 37.0 37.0 35.0 37.0 9 37.91256593526481 39.0 38.0 39.0 35.0 39.0 10-11 37.88323373851108 39.0 38.0 39.0 35.0 39.0 12-13 37.822741391350434 39.0 38.0 39.0 35.0 39.0 14-15 39.08965695606554 40.0 38.5 41.0 36.0 41.0 16-17 39.03921504801557 40.0 38.0 41.0 36.0 41.0 18-19 39.053660408527804 40.0 38.5 41.0 36.0 41.0 20-21 39.07489422129632 40.0 39.0 41.0 35.5 41.0 22-23 39.09072046499398 40.0 39.0 41.0 35.5 41.0 24-25 39.04491888392087 40.0 39.0 41.0 35.0 41.0 26-27 38.87961801159942 40.0 38.0 41.0 35.0 41.0 28-29 38.796980163600324 40.0 38.0 41.0 35.0 41.0 30-31 38.58011894414061 40.0 38.0 41.0 34.5 41.0 32-33 38.37414762887353 40.0 38.0 41.0 34.0 41.0 34-35 38.163268456406556 40.0 38.0 41.0 34.0 41.0 36-37 38.079279733364935 40.0 38.0 41.0 34.0 41.0 38-39 38.03361929228592 40.0 38.0 41.0 34.0 41.0 40-41 37.90463208745288 40.0 37.5 41.0 33.0 41.0 42-43 37.70446327893383 40.0 37.0 41.0 33.0 41.0 44-45 37.42098805116439 40.0 37.0 41.0 33.0 41.0 46-47 37.020438342675696 40.0 37.0 41.0 31.5 41.0 48-49 36.61803118100352 40.0 36.0 41.0 31.0 41.0 50-51 36.31941277624657 39.5 36.0 41.0 30.0 41.0 52-53 35.88038983120674 39.0 35.0 40.5 29.0 41.0 54-55 35.58967755487548 39.0 35.0 40.0 28.0 41.0 56-57 35.34530319159487 38.5 35.0 40.0 28.0 41.0 58-59 34.99462523644128 38.0 34.5 40.0 26.5 41.0 60-61 34.60945673563284 38.0 34.0 40.0 26.0 41.0 62-63 34.19981328831773 37.0 34.0 40.0 26.0 41.0 64-65 33.791568779935034 37.0 33.5 39.5 24.5 41.0 66-67 33.299019191478905 36.0 33.0 39.0 23.0 40.5 68-69 32.79206671166193 35.5 32.0 39.0 22.0 40.0 70-71 32.22504530466657 35.0 31.5 38.0 20.5 40.0 72-73 31.77175475921517 35.0 31.0 37.0 18.5 39.5 74-75 31.238405031602646 35.0 31.0 37.0 17.5 39.0 76-77 29.993552826792488 33.5 29.5 35.5 16.5 37.5 78-79 30.687362706388633 34.0 31.0 36.0 16.5 38.0 80-81 30.519885226482643 34.0 31.0 35.5 12.0 37.0 82-83 30.096403956395626 34.0 30.0 35.0 7.5 37.0 84-85 29.622687274970716 34.0 30.0 35.0 4.5 36.5 86-87 29.113546489479603 34.0 29.0 35.0 2.0 36.0 88-89 28.55014055508961 34.0 29.0 35.0 2.0 35.5 90-91 28.06426243799377 33.0 27.5 35.0 2.0 35.0 92-93 27.488995657974307 33.0 26.5 35.0 2.0 35.0 94-95 26.80707358047497 33.0 25.5 35.0 2.0 35.0 96-97 26.05125314513311 32.0 24.5 34.0 2.0 35.0 98-99 25.097598178760034 32.0 20.5 34.0 2.0 35.0 100-101 21.61567147288612 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.06352583732734729 1101 2 0.052448112791061874 1101 3 0.1460196379929215 1101 4 0.07426707716665959 1101 5 0.029797588260869645 1101 6 -0.006146724371212997 1101 7 0.03689792620038901 1101 8 0.11953449287140927 1101 9 0.12321790002371102 1101 10-11 0.14731333542307823 1101 12-13 0.09692249159994759 1101 14-15 0.24546351810946732 1101 16-17 0.24898933829459935 1101 18-19 0.20269122444152288 1101 20-21 0.15365038381647622 1101 22-23 0.17089222004559446 1101 24-25 0.17594614684395538 1101 26-27 0.24638800822250317 1101 28-29 0.1575815559353444 1101 30-31 0.08504318100764863 1101 32-33 0.247726260617803 1101 34-35 0.13263663078773646 1101 36-37 0.2069244799596177 1101 38-39 0.38847798732056305 1101 40-41 0.35752013149505757 1101 42-43 0.39842523939879726 1101 44-45 0.3954802467183214 1101 46-47 0.4820221034995882 1101 48-49 0.5945885844202721 1101 50-51 0.4801142996110883 1101 52-53 0.31800240039785166 1101 54-55 0.38951033836583093 1101 56-57 0.40043427779325924 1101 58-59 0.25585834252431283 1101 60-61 0.3578075688935911 1101 62-63 0.4402180312376842 1101 64-65 0.4365371364972077 1101 66-67 0.37625398081198114 1101 68-69 0.4269697896593101 1101 70-71 0.558974048649489 1101 72-73 0.4486049170398516 1101 74-75 0.5879524270434118 1101 76-77 0.6548046096371927 1101 78-79 0.7442193892735354 1101 80-81 0.824538573068903 1101 82-83 0.9133502537899396 1101 84-85 1.1138635416138278 1101 86-87 1.0588080203476338 1101 88-89 1.2275392119030677 1101 90-91 1.4119136300320534 1101 92-93 1.6551504204342216 1101 94-95 1.5849136521854668 1101 96-97 1.7893757151968437 1101 98-99 2.1666103583383887 1101 100-101 2.4757415051975293 1102 1 0.13583975594302444 1102 2 0.08675337937424388 1102 3 0.20959390502827802 1102 4 0.15425006046973522 1102 5 -0.02837697656669036 1102 6 0.06316250760946929 1102 7 0.1534823032385475 1102 8 0.11139521515131179 1102 9 0.11796019144591696 1102 10-11 0.14018533971669456 1102 12-13 0.10481750646842869 1102 14-15 0.18237228297618202 1102 16-17 0.11919955366569468 1102 18-19 0.18944623330908428 1102 20-21 0.19958391445710078 1102 22-23 0.20199474141859497 1102 24-25 0.12467602227553698 1102 26-27 0.16954071758210887 1102 28-29 0.08054465872154992 1102 30-31 0.16356918006947296 1102 32-33 0.23619153204679577 1102 34-35 0.11916413994054409 1102 36-37 0.07076921105213785 1102 38-39 0.12472974991509744 1102 40-41 0.1995561623786628 1102 42-43 0.15107261765827928 1102 44-45 0.22269033953539008 1102 46-47 0.2887186245147859 1102 48-49 0.28229881035637305 1102 50-51 0.4541543987248957 1102 52-53 0.33016835263558875 1102 54-55 0.3730280896825846 1102 56-57 0.41026254720363653 1102 58-59 0.4348363828149999 1102 60-61 0.4350474550169494 1102 62-63 0.40346492407052637 1102 64-65 0.5044936255920192 1102 66-67 0.48742823150681147 1102 68-69 0.4367687587968234 1102 70-71 0.6076029226597228 1102 72-73 0.5122976529669145 1102 74-75 0.5397065695807903 1102 76-77 0.5402489947611109 1102 78-79 0.534894859169615 1102 80-81 0.7534477999783853 1102 82-83 0.8443154301339142 1102 84-85 0.8711385317179428 1102 86-87 1.0111866249503123 1102 88-89 1.11923342974778 1102 90-91 1.2010525260034086 1102 92-93 1.2819703125021604 1102 94-95 1.3872526028130991 1102 96-97 1.602543229646244 1102 98-99 1.6841830561187514 1102 100-101 1.9009965091347532 1103 1 0.2557042486722523 1103 2 0.2404063595603958 1103 3 0.3706018254268102 1103 4 0.1540647117770746 1103 5 0.05609243202979286 1103 6 0.09094960712325673 1103 7 0.12989849214362437 1103 8 0.14667264630531207 1103 9 0.23081473869734737 1103 10-11 0.2968011839166351 1103 12-13 0.21045992612320674 1103 14-15 0.3484857996381976 1103 16-17 0.22725766048083784 1103 18-19 0.3754747791798039 1103 20-21 0.2841910675409949 1103 22-23 0.3541260160782045 1103 24-25 0.28010176134440457 1103 26-27 0.27432209843711064 1103 28-29 0.33880869445040673 1103 30-31 0.1983160867916638 1103 32-33 0.2590024535737072 1103 34-35 0.34697994678114696 1103 36-37 0.2904298564203529 1103 38-39 0.23317629256467143 1103 40-41 0.3413332508909903 1103 42-43 0.40770797415223825 1103 44-45 0.3798363461579868 1103 46-47 0.2713852636687548 1103 48-49 0.37733997260851737 1103 50-51 0.39017488989169635 1103 52-53 0.41505352875847024 1103 54-55 0.5125236154303039 1103 56-57 0.4150122884575822 1103 58-59 0.42884458811474957 1103 60-61 0.42837707413625026 1103 62-63 0.44698349387367386 1103 64-65 0.5327010177467741 1103 66-67 0.6037882945108848 1103 68-69 0.7073753160136391 1103 70-71 0.7298993929617623 1103 72-73 0.6886084248030357 1103 74-75 0.7508614475999913 1103 76-77 0.47926366373449625 1103 78-79 0.8007695945861677 1103 80-81 0.8006391337685734 1103 82-83 0.7920498138730387 1103 84-85 1.0591945099191982 1103 86-87 0.845953695583006 1103 88-89 0.7855380872490976 1103 90-91 0.7570023095400273 1103 92-93 0.973206360216416 1103 94-95 0.9053546901217437 1103 96-97 1.1057600430895285 1103 98-99 1.5603952890600645 1103 100-101 1.8989392110480807 1104 1 0.03222505155610378 1104 2 0.03561466187450435 1104 3 0.07044108254552839 1104 4 0.018926236899858395 1104 5 -0.06759653100136376 1104 6 -0.006702452907674683 1104 7 -0.004905734776301074 1104 8 -0.05190710373798879 1104 9 0.0510622645028036 1104 10-11 0.0033188165092568056 1104 12-13 -0.014044804498261954 1104 14-15 0.027609786385781376 1104 16-17 0.08178231430630944 1104 18-19 0.06004700763119075 1104 20-21 0.06349676363262802 1104 22-23 0.11718979362707671 1104 24-25 0.04409383662919453 1104 26-27 0.013169105044077867 1104 28-29 -0.08508709716524265 1104 30-31 -0.054589591473408916 1104 32-33 -0.005996923509648866 1104 34-35 0.0472987990749445 1104 36-37 0.12593630791236876 1104 38-39 0.1189095768749695 1104 40-41 0.15761538047588886 1104 42-43 0.168982005820709 1104 44-45 0.21437449144644916 1104 46-47 0.21424608180646487 1104 48-49 0.052136967618089614 1104 50-51 0.1364427075867809 1104 52-53 0.292559755392773 1104 54-55 0.3770868495730255 1104 56-57 0.4277030756722908 1104 58-59 0.4205197882330509 1104 60-61 0.5432155564074392 1104 62-63 0.43962801306810917 1104 64-65 0.42987115299564493 1104 66-67 0.39093716956393365 1104 68-69 0.5296207330183549 1104 70-71 0.5274894651897029 1104 72-73 0.5340238089197769 1104 74-75 0.6398323791693024 1104 76-77 0.4447753517778388 1104 78-79 0.6380242641820608 1104 80-81 0.7712364551256989 1104 82-83 0.6731335065229267 1104 84-85 0.8141755641372548 1104 86-87 0.9267935897985922 1104 88-89 0.9531290001613009 1104 90-91 1.0327396554694417 1104 92-93 1.1446380860196506 1104 94-95 1.104535073615569 1104 96-97 1.5003579883761766 1104 98-99 1.6539857299736376 1104 100-101 2.092830880272249 1105 1 0.16443985851371679 1105 2 0.11097250076856113 1105 3 0.22764291258786074 1105 4 0.10002862069671181 1105 5 0.09414400398000566 1105 6 0.10422851427109947 1105 7 0.09754770909923849 1105 8 0.05703977256865045 1105 9 0.10176796844859126 1105 10-11 0.06618508524145739 1105 12-13 0.08537304829122405 1105 14-15 0.17072665821996225 1105 16-17 0.19423423177142496 1105 18-19 0.2256431242877781 1105 20-21 0.14412496524207796 1105 22-23 0.1940907873469513 1105 24-25 0.2608301624549654 1105 26-27 0.19291861357147155 1105 28-29 0.19271984176548074 1105 30-31 0.16714627448786246 1105 32-33 0.1076732102199216 1105 34-35 0.26235668012122204 1105 36-37 0.30573338026203345 1105 38-39 0.1838031321954574 1105 40-41 0.35276755922655667 1105 42-43 0.29561728521812114 1105 44-45 0.3557177908565876 1105 46-47 0.2234504094348111 1105 48-49 0.34458773966871803 1105 50-51 0.3372110574546099 1105 52-53 0.18700677621092154 1105 54-55 0.26839999691726035 1105 56-57 0.3257075549224524 1105 58-59 0.24668603010139378 1105 60-61 0.22158462206225238 1105 62-63 0.3721513296546206 1105 64-65 0.39634047833438046 1105 66-67 0.5366621647650902 1105 68-69 0.5371051188589959 1105 70-71 0.6982110690417969 1105 72-73 0.530767143129161 1105 74-75 0.49366632658555787 1105 76-77 0.6201360056736185 1105 78-79 0.7460964272366688 1105 80-81 0.8835225058942591 1105 82-83 0.7834730806665533 1105 84-85 0.8554084232641337 1105 86-87 1.0665712871708593 1105 88-89 1.1368010679911933 1105 90-91 1.0738508143061551 1105 92-93 1.276637521077923 1105 94-95 1.307485413830367 1105 96-97 1.5411319536871027 1105 98-99 1.95153323252109 1105 100-101 2.3829421674827493 1106 1 0.031179190138125534 1106 2 0.05210725757764578 1106 3 0.13577476043170122 1106 4 0.03647187220504122 1106 5 0.04801027678839631 1106 6 -0.023590566552684322 1106 7 0.042272162159328275 1106 8 -0.0405239394508925 1106 9 -0.04838860441637394 1106 10-11 0.04068844274121375 1106 12-13 0.032123480848916586 1106 14-15 0.046586033774708824 1106 16-17 0.08906673549343935 1106 18-19 0.014154723461246022 1106 20-21 -0.01958030852966175 1106 22-23 0.011056753163650512 1106 24-25 0.15135779999174304 1106 26-27 0.10004451330330255 1106 28-29 -0.07860483804715557 1106 30-31 0.04798414642237958 1106 32-33 0.10211765466436162 1106 34-35 -6.460868278139742E-4 1106 36-37 0.08672368292223354 1106 38-39 0.014689881373534774 1106 40-41 0.07697386616617763 1106 42-43 -0.001448541659392788 1106 44-45 0.06220201403978365 1106 46-47 -0.13386354342054574 1106 48-49 0.09318864336515986 1106 50-51 0.11049141874383395 1106 52-53 0.3321158564903115 1106 54-55 0.223006410842423 1106 56-57 0.3992925409693626 1106 58-59 0.37895128323344096 1106 60-61 0.35577845524529295 1106 62-63 0.378534129598755 1106 64-65 0.43014033489801307 1106 66-67 0.489754922648757 1106 68-69 0.15438044330684164 1106 70-71 0.2702997960322051 1106 72-73 0.28672495958268485 1106 74-75 0.20587167254886296 1106 76-77 0.2929739864872296 1106 78-79 0.6097379120634194 1106 80-81 0.6482888774039139 1106 82-83 0.541520783909494 1106 84-85 0.6669965904740103 1106 86-87 0.8223711105973806 1106 88-89 0.89888238646963 1106 90-91 0.6584160198627771 1106 92-93 0.6513234877467191 1106 94-95 0.9308592691205924 1106 96-97 1.0849144842738419 1106 98-99 1.2852004222807771 1106 100-101 2.0066094045811695 1107 1 0.09008488221962097 1107 2 0.09430427501095551 1107 3 0.22562983289546423 1107 4 0.06991022549216552 1107 5 0.0563488611194245 1107 6 0.047812500029962735 1107 7 -0.021716495685659254 1107 8 0.06763611095868782 1107 9 0.07902160352313814 1107 10-11 0.10879388277481894 1107 12-13 0.07822724056325825 1107 14-15 0.08442472312569294 1107 16-17 0.18969160421850262 1107 18-19 0.10905075370698825 1107 20-21 0.11068119115528674 1107 22-23 0.11660180672382836 1107 24-25 0.11879111232109096 1107 26-27 0.15931252422537057 1107 28-29 0.09424750389781877 1107 30-31 0.05569786202833882 1107 32-33 0.041510639331455934 1107 34-35 0.052100184963826734 1107 36-37 0.12938803914992292 1107 38-39 0.11292554614984596 1107 40-41 0.2552189827383202 1107 42-43 0.035880800915698785 1107 44-45 0.08946519404398856 1107 46-47 0.1877863200535188 1107 48-49 0.1839363854361622 1107 50-51 0.18931939605951698 1107 52-53 0.017822640779328935 1107 54-55 -0.03668693089250752 1107 56-57 -0.03742768587373746 1107 58-59 0.003704486342037683 1107 60-61 0.07194627713144541 1107 62-63 0.11770132136519607 1107 64-65 0.09491664239644138 1107 66-67 0.2895816397250002 1107 68-69 0.3909851482007767 1107 70-71 0.4143031566707336 1107 72-73 0.4292002798935428 1107 74-75 0.5019754322632117 1107 76-77 0.4689821780436283 1107 78-79 0.5250603233215578 1107 80-81 0.5365108895862285 1107 82-83 0.8089751002295955 1107 84-85 0.7982505287222814 1107 86-87 0.8177922406120821 1107 88-89 0.9275843398407702 1107 90-91 0.8263481769726297 1107 92-93 1.0098931159760838 1107 94-95 1.1603436211474758 1107 96-97 1.1463196512555704 1107 98-99 1.3511214472755277 1107 100-101 1.737805581854854 1108 1 -0.013349589537753559 1108 2 0.08130816457079248 1108 3 0.09186058209940029 1108 4 0.06416208820559177 1108 5 -0.021051376938707733 1108 6 0.023412194925469976 1108 7 0.04170740538525308 1108 8 -0.023415681051304205 1108 9 -0.008178723175511493 1108 10-11 0.08022364213061195 1108 12-13 0.08615199016249875 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-1.382588488085755 2316 86-87 -1.4889589322057333 2316 88-89 -1.754963994274867 2316 90-91 -2.02052065381757 2316 92-93 -2.3854206571414878 2316 94-95 -2.4757339177775926 2316 96-97 -2.6544017802533233 2316 98-99 -3.2492967230859335 2316 100-101 -3.8151192381078864 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 5.0 4 6.0 5 39.0 6 64.0 7 180.0 8 400.0 9 840.0 10 1414.0 11 2406.0 12 3836.0 13 5891.0 14 7811.0 15 10591.0 16 14458.0 17 21194.0 18 20770.0 19 13516.0 20 11060.0 21 11582.0 22 11902.0 23 13075.0 24 14580.0 25 17469.0 26 20363.0 27 24143.0 28 29463.0 29 36079.0 30 44239.0 31 54373.0 32 69471.0 33 91030.0 34 128585.0 35 192662.0 36 302061.0 37 448358.0 38 319774.0 39 22441.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.17352895251745 19.259734717844623 14.98962684620013 22.577109483437795 2 15.51785090260402 20.668205214548077 40.02404977204092 23.789894110806987 3 19.104085728789315 24.96238582227797 30.27099501834677 25.66253343058595 4 12.800717347563642 17.288818858550698 36.93002300964533 32.98044078424033 5 13.534148168756483 37.699726822787795 33.93119352146331 14.83493148699242 6 31.345860806904113 36.46291296820356 17.330830279960857 14.86039594493147 7 27.990781905395007 33.75656682431962 20.448464066266116 17.804187204019257 8 26.46806013024558 34.741512777372016 19.691251655534828 19.099175436847577 9 26.78180977583917 16.57049650252552 20.306357708252698 36.341336013382616 10-11 25.129225744138367 26.794245353696827 26.83653649066467 21.23999241150014 12-13 25.86068694233808 24.357456998076934 27.85775428213656 21.924101777448424 14-15 22.77844377770985 25.518644974678722 26.478371503860625 25.224539743750807 16-17 21.63859209931373 28.875793244912728 27.203576767187165 22.282037888586377 18-19 21.52893522462621 26.921042472711665 30.26857796496982 21.281444337692314 20-21 22.461781578357108 26.10939849949113 30.228499292774192 21.200320629377565 22-23 22.140669018830366 25.997503729401828 30.1745100662061 21.687317185561707 24-25 21.88581772461449 26.26343902808705 30.27004702706167 21.580696220236796 26-27 21.56696808984191 26.518269805537535 30.261843895862743 21.652918208757807 28-29 21.40390183240914 26.616127387465973 30.43729361396959 21.542677166155297 30-31 21.64016538063842 26.37270863436872 30.205744174726913 21.781381810265948 32-33 21.50577258431264 26.44369285685766 30.148029524015158 21.902505034814542 34-35 22.01668181322199 26.55683315668711 29.8313886987621 21.5950963313288 36-37 22.09258587175437 26.260987263535878 29.895377318925853 21.7510495457839 38-39 21.65171979531812 26.506110212498495 29.95600260367317 21.88616738851021 40-41 21.909393839188056 26.38432579553837 29.898125302062933 21.808155063210645 42-43 21.935667171648827 26.36454557201185 29.608174199515098 22.091613056824226 44-45 21.790621522810362 26.695991098394533 29.533085360038147 21.98030201875695 46-47 22.035992711431433 26.902040468594436 29.01531845526088 22.046648364713256 48-49 22.12409365005737 26.80438404824101 28.92403241294565 22.147489888755974 50-51 22.10766006165402 27.1442216777807 28.658412223384683 22.089706037180598 52-53 22.29243901607877 27.158183093412298 28.39225525434953 22.157122636159404 54-55 21.91904108216484 27.117646669884486 28.539040848203044 22.424271399747624 56-57 21.961154204725723 27.350260638522418 28.476150901286946 22.212434255464917 58-59 21.959730089470042 26.991892206681843 28.530877616112438 22.517500087735673 60-61 21.976107414910384 27.071261201556556 28.744316890057792 22.208314493475264 62-63 21.822633565481073 27.156886131304443 28.593411534214624 22.42706876899986 64-65 21.601031059445113 27.053256315824004 28.560885759000026 22.784826865730853 66-67 21.638647127755668 27.26879938640821 28.370024738979353 22.722528746856767 68-69 21.68125794390907 27.10374039546744 28.46491761222178 22.750084048401707 70-71 21.656571554416715 27.20693361028984 28.231787981789637 22.904706853503807 72-73 21.88173434859188 27.111161859345223 28.078644730544678 22.928459061518218 74-75 21.784080731059394 27.44643927954009 27.81004642107121 22.959433568329306 76-77 21.708348316212586 27.228931104864216 28.11943546036814 22.94328511855505 78-79 21.76500775888508 27.275240281303454 28.083069660089123 22.876682299722347 80-81 21.709571160205286 27.539830138436894 27.985782225544032 22.764816475813788 82-83 21.74426083093245 27.500150677744685 27.873882346239405 22.88170614508347 84-85 21.754747628569923 27.249542931155986 27.83716472611092 23.15854471416317 86-87 21.75715203403253 27.09744425439152 27.997110042677985 23.148293668897963 88-89 21.665296377834533 27.12047852367887 27.8719800135342 23.3422450849524 90-91 21.589560065633606 27.20122315324104 27.96978839977905 23.239428381346304 92-93 21.683907017054878 26.85748711678409 27.99070658819078 23.46789927797026 94-95 21.51467603871565 26.990560135901493 27.906343934531286 23.588419890851572 96-97 21.581113931377256 26.882120758760948 27.995095941181813 23.54166936867999 98-99 21.446629024181775 26.920734366683373 27.752978631366855 23.879657977767994 100-101 21.606547037760418 26.586634318033852 27.885716756184898 23.921101888020832 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1092.0 1 759.5 2 561.0 3 724.0 4 607.5 5 616.0 6 1241.0 7 2598.5 8 4091.5 9 4717.0 10 4029.0 11 3259.0 12 3208.0 13 3382.5 14 3680.0 15 3745.5 16 3729.0 17 3847.0 18 3978.0 19 4217.5 20 4504.0 21 5071.5 22 5979.0 23 7035.5 24 8265.5 25 10169.5 26 12347.5 27 14799.0 28 17466.5 29 19939.0 30 23085.5 31 26829.5 32 30492.0 33 33080.0 34 35189.5 35 37636.0 36 41177.0 37 45372.5 38 48576.0 39 50458.0 40 52661.5 41 57880.5 42 63043.0 43 66370.5 44 69733.5 45 73339.5 46 74346.0 47 75320.0 48 79950.0 49 80164.0 50 76473.5 51 73745.5 52 71121.5 53 69627.0 54 67631.5 55 61787.0 56 53721.0 57 47623.0 58 43274.5 59 37406.5 60 30577.0 61 24972.0 62 20062.5 63 15202.0 64 11492.0 65 8769.5 66 6534.0 67 5505.0 68 4351.5 69 2964.5 70 2161.5 71 1708.0 72 1490.5 73 1261.0 74 1000.0 75 810.5 76 507.0 77 242.5 78 124.0 79 80.0 80 37.0 81 18.0 82 10.5 83 4.5 84 5.0 85 5.5 86 2.5 87 1.5 88 1.0 89 1.0 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.010680864417615695 3 0.006052489836648894 4 5.086125913150331E-5 5 5.086125913150332E-4 6 5.086125913150331E-5 7 0.0 8 5.086125913150331E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0015766990330766026 26-27 0.023294456682228515 28-29 2.797369252232682E-4 30-31 1.0172251826300662E-4 32-33 0.00368744128703399 34-35 0.017267397475145375 36-37 0.008315815868000792 38-39 0.022811274720479235 40-41 0.0245659881605161 42-43 0.01968330728389178 44-45 0.009943376160208899 46-47 0.002059880994825884 48-49 2.543062956575166E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.543062956575166E-4 68-69 2.0344503652601324E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.271531478287583E-4 82-83 8.392107756698047E-4 84-85 0.00368744128703399 86-87 7.120576278410464E-4 88-89 1.271531478287583E-4 90-91 0.003407704361810722 92-93 4.577513321835298E-4 94-95 1.5258377739450996E-4 96-97 3.3059818435477156E-4 98-99 1.780144069602616E-4 100-101 0.0026956467339696753 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1966133.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.47052675399777 #Duplication Level Percentage of deduplicated Percentage of total 1 70.30359556900957 33.37348714360907 2 15.287900949260244 14.514494220486526 3 5.719099847079185 8.14466046898671 4 2.8297737323274332 5.373233986728382 5 1.5605276958811314 3.7039535868839866 6 0.9979928873410742 2.8425148835294363 7 0.649169277530516 2.1571485289820034 8 0.4441485601292716 1.686717288509294 9 0.34195912209628976 1.4609681688820961 >10 1.6832998935443413 14.259985308889997 >50 0.11399665877199652 3.7201218062552934 >100 0.06402492727034959 5.632791672231001 >500 0.003114655071471353 0.9902670334979152 >1k 0.0010740189901625352 0.7169914684694013 >5k 2.1480379803250706E-4 0.8229877184123642 >10k+ 1.0740189901625353E-4 0.5996767156465262 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11762 0.5982301299047419 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9606 0.48857325521722084 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6536 0.33242918968350565 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2232 0.1135223303815154 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.5430629565751655E-5 0.0 12-13 0.0 0.0 0.0 5.086125913150331E-5 0.0 14-15 0.0 0.0 0.0 1.0172251826300662E-4 0.0 16-17 0.0 0.0 0.0 1.780144069602616E-4 0.0 18-19 0.0 0.0 0.0 2.288756660917649E-4 0.0 20-21 0.0 0.0 0.0 2.797369252232682E-4 0.0 22-23 0.0 0.0 0.0 4.831819617492815E-4 0.0 24-25 0.0 0.0 0.0 8.13780146104053E-4 0.0 26-27 0.0 0.0 0.0 0.0022378954017861457 0.0 28-29 0.0 0.0 0.0 0.007120576278410464 0.0 30-31 0.0 0.0 0.0 0.02184491079698067 0.0 32-33 0.0 0.0 0.0 0.05315001579242096 0.0 34-35 0.0 0.0 0.0 0.09986608230470675 0.0 36-37 0.0 0.0 0.0 0.17478471700541115 0.0 38-39 0.0 0.0 0.0 0.2824071413276721 0.0 40-41 0.0 0.0 0.0 0.4048047614276349 0.0 42-43 0.0 0.0 0.0 0.5279144391554387 0.0 44-45 0.0 0.0 0.0 0.6703513953532136 0.0 46-47 0.0 0.0 0.0 0.8303863472104889 0.0 48-49 0.0 0.0 0.0 0.9922268737669324 0.0 50-51 0.0 0.0 0.0 1.1729877887202953 0.0 52-53 0.0 0.0 0.0 1.3443393707343296 0.0 54-55 0.0 0.0 0.0 1.5312799286721703 0.0 56-57 0.0 0.0 0.0 1.7339620463112109 0.0 58-59 0.0 0.0 0.0 1.9550050784967241 0.0 60-61 0.0 0.0 0.0 2.1929340487138966 0.0 62-63 0.0 0.0 0.0 2.4176136609273127 0.0 64-65 0.0 0.0 0.0 2.650914256563518 0.0 66-67 0.0 0.0 0.0 2.9086536872124116 0.0 68-69 0.0 0.0 0.0 3.186941066550432 0.0 70-71 0.0 0.0 0.0 3.4819618001427166 0.0 72-73 0.0 0.0 0.0 3.7806954056516013 0.0 74-75 0.0 0.0 0.0 4.084133677630151 0.0 76-77 0.0 0.0 0.0 4.396599823104541 0.0 78-79 0.0 0.0 0.0 4.735640976475143 0.0 80-81 0.0 0.0 0.0 5.098205462194063 0.0 82-83 0.0 0.0 0.0 5.46875516559663 0.0 84-85 0.0 0.0 0.0 5.836558361006096 0.0 86-87 0.0 0.0 0.0 6.220535436819381 0.0 88-89 0.0 0.0 0.0 6.635334435666357 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6125 0.0 47.616848 1 GTATCAA 10040 0.0 37.990955 1 TATCAAC 12625 0.0 30.024092 2 ATCAACG 12840 0.0 29.371881 3 CAACGCA 13165 0.0 28.753561 5 TCAACGC 13200 0.0 28.713303 4 AACGCAG 13510 0.0 28.089603 6 GTACATG 9180 0.0 25.457794 1 TACATGG 9370 0.0 24.536022 2 ACGCAGA 15350 0.0 24.50592 7 CGCAGAG 15380 0.0 24.30371 8 ACATGGG 9335 0.0 23.405602 3 GTAAGGT 615 0.0 23.168629 4 GCAGAGT 16245 0.0 22.658764 9 GAGTACT 9365 0.0 22.416546 12-13 GTACTTT 9905 0.0 20.97866 14-15 AGTACTT 9625 0.0 20.972124 12-13 CATGGGG 4565 0.0 20.912668 4 TAAGGTG 830 0.0 20.60054 5 CAACGCG 165 6.3300606E-5 20.149687 9 >>END_MODULE