FastQCFastQC Report
Thu 26 May 2016
SRR938105_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938105_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2014763
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128810.6393307798485479No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99950.49608812550160986No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66650.3308081397166813No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22340.11088152800106017No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63400.046.454991
GTATCAA105900.038.7649231
TATCAAC134750.030.2607882
ATCAACG134750.030.155733
CAACGCA138650.029.2389435
TCAACGC139600.029.1080534
AACGCAG141450.028.6937586
GTACATG97800.026.0150591
ACGCAGA160800.025.1622097
CGCAGAG161050.025.0641068
TACATGG100450.024.7442022
ACATGGG101200.023.5752183
GCAGAGT167300.023.4717869
GAGTACT96100.022.60705812-13
GTACTTT101900.021.09646414-15
CATGGGG49850.021.0697634
AGTACTT100250.020.77021412-13
AGAGTAC154200.020.48673210-11
TAGACCG1404.8355106E-420.3683435
CAGAGTA165000.020.1974310-11
Produced by FastQC (version 0.11.5)