FastQCFastQC Report
Thu 26 May 2016
SRR938105_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938105_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2014763
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125710.6239443547454464No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100340.4980238370468387No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67420.3346299291777743No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22170.11003775630185784No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70150.043.8841361
GTATCAA110450.038.4101451
TAGCGCG1401.708031E-830.5343364
TATCAAC140600.030.1405052
ATCAACG141600.029.8218143
TCAACGC143950.029.3665084
CAACGCA144350.029.2851315
AACGCAG147500.028.6919256
GTACATG96550.025.7341381
ACGCAGA167400.025.1959937
CGCAGAG166800.025.1727228
TACATGG97500.024.9480362
GAGTACT99300.023.94025812-13
ACATGGG99200.023.7022233
GCAGAGT177400.023.3467319
GTACTTT106050.022.21493314-15
CGCGTGC1954.2275497E-721.9215419
AGTACTT102350.021.34735112-13
CATGGGG49100.020.8951854
CGCGCGT2056.837454E-720.8527167
Produced by FastQC (version 0.11.5)