##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938105_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2014763 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.532969882810036 34.0 31.0 34.0 31.0 34.0 2 32.73977237024901 34.0 31.0 34.0 31.0 34.0 3 32.796427172823805 34.0 31.0 34.0 31.0 34.0 4 36.25319007744335 37.0 35.0 37.0 35.0 37.0 5 36.22102103324312 37.0 37.0 37.0 35.0 37.0 6 36.25901210216785 37.0 37.0 37.0 35.0 37.0 7 36.22359602593456 37.0 37.0 37.0 35.0 37.0 8 36.21834131359371 37.0 37.0 37.0 35.0 37.0 9 37.92674920077448 39.0 38.0 39.0 35.0 39.0 10-11 37.91897111471672 39.0 38.0 39.0 35.0 39.0 12-13 37.86909974026722 39.0 38.0 39.0 35.0 39.0 14-15 39.1813091663883 40.5 39.0 41.0 36.0 41.0 16-17 39.14520864240608 40.0 38.5 41.0 36.0 41.0 18-19 39.19049287682968 40.0 39.0 41.0 36.0 41.0 20-21 39.1351069579896 40.0 39.0 41.0 36.0 41.0 22-23 38.99779601868805 40.0 38.5 41.0 35.0 41.0 24-25 38.9592557536544 40.0 38.0 41.0 35.0 41.0 26-27 38.91920612995176 40.0 38.0 41.0 35.0 41.0 28-29 38.85774431037298 40.0 38.0 41.0 35.0 41.0 30-31 38.659114744513374 40.0 38.0 41.0 35.0 41.0 32-33 38.479896146593916 40.0 38.0 41.0 34.5 41.0 34-35 38.277986046001445 40.0 38.0 41.0 34.0 41.0 36-37 38.150795651895535 40.0 38.0 41.0 34.0 41.0 38-39 38.13605098962012 40.0 38.0 41.0 34.0 41.0 40-41 38.069534233058675 40.0 38.0 41.0 34.0 41.0 42-43 37.90038704304179 40.0 38.0 41.0 33.0 41.0 44-45 37.658599795608716 40.0 37.0 41.0 33.0 41.0 46-47 37.287784965278796 40.0 37.0 41.0 32.5 41.0 48-49 36.91344565092768 40.0 37.0 41.0 31.0 41.0 50-51 36.61884375482377 40.0 36.0 41.0 31.0 41.0 52-53 36.23412455956358 39.0 35.5 41.0 30.0 41.0 54-55 36.023199254701424 39.0 35.0 41.0 29.5 41.0 56-57 35.80527734527585 39.0 35.0 41.0 29.0 41.0 58-59 35.49699468374196 38.5 35.0 40.0 28.0 41.0 60-61 35.16138076786203 38.0 35.0 40.0 28.0 41.0 62-63 34.820932784650104 38.0 34.0 40.0 27.0 41.0 64-65 34.44580082123803 37.0 34.0 40.0 26.0 41.0 66-67 34.04165328626742 36.5 34.0 39.5 26.0 41.0 68-69 33.60666217316876 36.0 33.5 39.0 25.5 41.0 70-71 33.11207124609693 35.5 33.0 39.0 24.5 40.0 72-73 32.70876549748035 35.0 33.0 38.0 23.5 40.0 74-75 32.266597857911826 35.0 32.0 37.0 22.0 39.0 76-77 30.93229972954635 34.0 30.5 36.0 20.5 38.5 78-79 31.639363786212073 35.0 32.0 36.0 22.0 39.0 80-81 31.484367143927102 35.0 32.0 36.0 20.5 37.5 82-83 31.127692934603225 35.0 32.0 35.5 20.0 37.0 84-85 30.76764140496922 34.5 31.0 35.0 18.0 37.0 86-87 30.36003043534153 34.0 31.0 35.0 14.5 36.0 88-89 29.944978640167605 34.0 31.0 35.0 8.5 36.0 90-91 29.64103420600835 34.0 30.0 35.0 4.5 35.5 92-93 29.279497141847454 34.0 30.0 35.0 2.0 35.0 94-95 28.91331188829654 34.0 29.5 35.0 2.0 35.0 96-97 28.396575924811007 34.0 29.0 35.0 2.0 35.0 98-99 27.777687003384514 34.0 29.0 35.0 2.0 35.0 100-101 25.432084567763056 31.5 22.5 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09669717627338059 1101 2 0.12926354566595677 1101 3 0.10655340172709771 1101 4 0.017696341478455224 1101 5 0.03147660292199106 1101 6 0.06569993975181632 1101 7 0.0482810479741147 1101 8 0.0056291241419117455 1101 9 -0.044139698558311125 1101 10-11 0.06388362007744774 1101 12-13 0.18539440802100415 1101 14-15 0.10236464417718594 1101 16-17 0.17866647649037048 1101 18-19 0.1435116547072255 1101 20-21 0.11687081697572665 1101 22-23 0.27725492205760816 1101 24-25 0.18575737729909747 1101 26-27 0.1659203654282848 1101 28-29 0.2711267141449909 1101 30-31 0.22657223606859134 1101 32-33 0.1439030741548919 1101 34-35 0.12578371659179055 1101 36-37 0.18887457146822584 1101 38-39 0.2387575190401634 1101 40-41 0.2988729825008889 1101 42-43 0.2555957540806588 1101 44-45 0.23875582148861696 1101 46-47 0.261193451036668 1101 48-49 0.2432504009227543 1101 50-51 0.27197296312746033 1101 52-53 0.3630488610496698 1101 54-55 0.4149379640101287 1101 56-57 0.37144145636619896 1101 58-59 0.3770472137713483 1101 60-61 0.32138257665889114 1101 62-63 0.2257777809187047 1101 64-65 0.3113848158273598 1101 66-67 0.2890941439932817 1101 68-69 0.4289477543675204 1101 70-71 0.5377331961294374 1101 72-73 0.43285033254272065 1101 74-75 0.4378929920457679 1101 76-77 0.4553955484651411 1101 78-79 0.2812719769478349 1101 80-81 0.481376006047487 1101 82-83 0.508402344268756 1101 84-85 0.5525423732276913 1101 86-87 0.5419460770446136 1101 88-89 0.47254884574359224 1101 90-91 0.5059379716512673 1101 92-93 0.7208054808365496 1101 94-95 0.4590022266292735 1101 96-97 0.36039686190254727 1101 98-99 0.48828022906967306 1101 100-101 0.6405168071652234 1102 1 0.19391258073702744 1102 2 0.17364903214975413 1102 3 0.17076587686022293 1102 4 0.1771530108587882 1102 5 0.1313802961651831 1102 6 0.12400538058885502 1102 7 0.11300281654435906 1102 8 0.16579819966850806 1102 9 0.04684407606189467 1102 10-11 0.12633624314478453 1102 12-13 0.07610822688624097 1102 14-15 0.17043351230343973 1102 16-17 0.13928858405819966 1102 18-19 0.15207427086487968 1102 20-21 0.13597605464507723 1102 22-23 0.2284162100395548 1102 24-25 0.112805146439932 1102 26-27 -0.012703932128808049 1102 28-29 0.04686898253191174 1102 30-31 -0.035180541408387 1102 32-33 0.10308415339523691 1102 34-35 0.0817531963649003 1102 36-37 0.12407873134844039 1102 38-39 0.06122766641918531 1102 40-41 0.1407412181468075 1102 42-43 0.12272747280995588 1102 44-45 0.02747160496771528 1102 46-47 -0.058125288598127156 1102 48-49 -0.09354097896287783 1102 50-51 -0.12852448435869945 1102 52-53 -0.11717760824678436 1102 54-55 -0.06927558852735416 1102 56-57 0.06674367103681789 1102 58-59 -0.03815268046712106 1102 60-61 -0.06786808861081539 1102 62-63 -0.027380772874828097 1102 64-65 0.022793263666976316 1102 66-67 -0.039080749516841706 1102 68-69 0.07837618036221272 1102 70-71 0.031234276557128737 1102 72-73 -0.015234306364661165 1102 74-75 -0.06313466481413599 1102 76-77 -0.009001184800872863 1102 78-79 0.03732330647030935 1102 80-81 0.13706066078611556 1102 82-83 0.23237835663032058 1102 84-85 0.236794184613764 1102 86-87 0.04575849434970891 1102 88-89 -0.15616208391894304 1102 90-91 -0.05452447668416838 1102 92-93 0.04186565771776074 1102 94-95 -0.041154849031446616 1102 96-97 0.04469416064094034 1102 98-99 0.14056666251494576 1102 100-101 0.21849463462331542 1103 1 0.09826423227283954 1103 2 0.1562153535991655 1103 3 0.08244664241713906 1103 4 0.11198597225496343 1103 5 0.1113747389190678 1103 6 0.0753562117566986 1103 7 0.10196102853875999 1103 8 0.009924584452548402 1103 9 0.058817006827119656 1103 10-11 0.03540256499076122 1103 12-13 0.10419307350281315 1103 14-15 0.17352357379535022 1103 16-17 0.15636958105247345 1103 18-19 0.0772185358635582 1103 20-21 0.21703610330667544 1103 22-23 0.19334435579908416 1103 24-25 0.23654283596330572 1103 26-27 0.1868316068830822 1103 28-29 0.18019067437759162 1103 30-31 0.18006373551379085 1103 32-33 0.13939832445213796 1103 34-35 0.19501989209562964 1103 36-37 0.13284950393519068 1103 38-39 0.19342310381966143 1103 40-41 0.3691975701029122 1103 42-43 0.27734965013475943 1103 44-45 0.22036382902678753 1103 46-47 0.2490450129710382 1103 48-49 0.38015542778311584 1103 50-51 0.31201641916703693 1103 52-53 0.2539197945205771 1103 54-55 0.34469046891320687 1103 56-57 0.24788120917679635 1103 58-59 0.31283103405223045 1103 60-61 0.3562698178090713 1103 62-63 0.401984566882561 1103 64-65 0.34058128936143106 1103 66-67 0.17696168753915487 1103 68-69 0.3582858367779309 1103 70-71 0.39371702252409335 1103 72-73 0.30259443369237715 1103 74-75 0.3016787140311621 1103 76-77 0.33936636754418004 1103 78-79 0.35425685930869477 1103 80-81 0.43190111997009595 1103 82-83 0.5744792018763505 1103 84-85 0.6141750825452341 1103 86-87 0.6135329706215629 1103 88-89 0.5788722813824769 1103 90-91 0.39571794561947726 1103 92-93 0.6368607257279315 1103 94-95 0.485239822780386 1103 96-97 0.722023055227492 1103 98-99 0.7221866658514102 1103 100-101 0.652022092627373 1104 1 0.05077996570364718 1104 2 0.024525553345327467 1104 3 -0.03247252717677185 1104 4 -0.027820945507130546 1104 5 -0.04902245132696237 1104 6 0.005902441740943232 1104 7 0.006770869341579555 1104 8 -0.04934094981770443 1104 9 -0.01921480231801098 1104 10-11 -0.037569960445750894 1104 12-13 -0.009174713679627189 1104 14-15 0.0638405091562646 1104 16-17 0.01963728311839219 1104 18-19 0.04473315359142305 1104 20-21 0.08710012749066465 1104 22-23 0.05003193474389889 1104 24-25 -0.0016674402160816726 1104 26-27 -0.06967897412048529 1104 28-29 -0.016698079437752256 1104 30-31 0.06090704033696426 1104 32-33 0.043454813350130905 1104 34-35 0.028059869599822207 1104 36-37 0.11834788995225409 1104 38-39 0.08689808430748514 1104 40-41 0.024574475758868175 1104 42-43 0.09072725651273572 1104 44-45 0.04837986182945997 1104 46-47 0.03297878969111423 1104 48-49 -0.043311641815542146 1104 50-51 -0.03267066402199248 1104 52-53 -0.13927562917897518 1104 54-55 -0.08342481601510343 1104 56-57 0.016933603798392483 1104 58-59 0.03891523478990422 1104 60-61 0.014518901266683315 1104 62-63 -0.023945631510621013 1104 64-65 0.0368955318900106 1104 66-67 0.06891469271782569 1104 68-69 0.05337962145433295 1104 70-71 0.13295515896699328 1104 72-73 0.03996818508952771 1104 74-75 -0.1671885041932697 1104 76-77 -0.1628214956272238 1104 78-79 0.008504936109442696 1104 80-81 -0.04994005744484298 1104 82-83 -0.09937811132923002 1104 84-85 -0.04838296177391399 1104 86-87 -0.1687123588238073 1104 88-89 -0.08727620605753472 1104 90-91 -0.19417628473228987 1104 92-93 -0.19836875568728374 1104 94-95 -0.24435366757256816 1104 96-97 -0.01920252173228576 1104 98-99 0.06694682505868599 1104 100-101 0.30812887499058306 1105 1 0.09354153850198088 1105 2 0.08036035585104884 1105 3 0.10126616308129144 1105 4 0.06112505916151889 1105 5 0.15029783402430752 1105 6 0.06297317650025747 1105 7 0.04434224803603115 1105 8 0.006372183092622663 1105 9 0.01914598602402151 1105 10-11 0.029929734177997602 1105 12-13 0.10391284707100823 1105 14-15 0.16148649102990476 1105 16-17 0.13846555682400918 1105 18-19 0.1538171594738884 1105 20-21 0.19366489947800147 1105 22-23 0.09892856464038147 1105 24-25 0.11002705175805261 1105 26-27 0.09533589553915789 1105 28-29 0.06510717034078795 1105 30-31 0.16712688142589371 1105 32-33 0.18015216672383616 1105 34-35 0.19873261589900437 1105 36-37 0.2533660345919131 1105 38-39 0.180459959308088 1105 40-41 0.1710010685966168 1105 42-43 0.23872545503327558 1105 44-45 0.22535802670571314 1105 46-47 0.2156962247998706 1105 48-49 0.25332243855949343 1105 50-51 0.1626085556767407 1105 52-53 0.22985164875959185 1105 54-55 0.29616424372418493 1105 56-57 0.2148783734966031 1105 58-59 0.3536257861888217 1105 60-61 0.3577146854392481 1105 62-63 0.4726834474264905 1105 64-65 0.3233274002829418 1105 66-67 0.3785089895397036 1105 68-69 0.3469089630631217 1105 70-71 0.5205441148302583 1105 72-73 0.2999719025044456 1105 74-75 0.3234396908358832 1105 76-77 0.3376124031571379 1105 78-79 0.28200315819444555 1105 80-81 0.37743485611203553 1105 82-83 0.5655010221908867 1105 84-85 0.5910031453857378 1105 86-87 0.5793861329541166 1105 88-89 0.4327275017392296 1105 90-91 0.3832576361738198 1105 92-93 0.43471169244155305 1105 94-95 0.166259095135171 1105 96-97 0.3553542017872644 1105 98-99 0.44600760791837146 1105 100-101 0.5334929460071542 1106 1 -0.11529244215506651 1106 2 -0.019956956076462973 1106 3 -0.02500494581406798 1106 4 8.599941053191174E-4 1106 5 -0.05036882757163852 1106 6 -0.03258364980885631 1106 7 -0.010428459696100845 1106 8 -0.10106396096473702 1106 9 -0.15080662347302365 1106 10-11 -0.029835519071127692 1106 12-13 -0.15653992687875729 1106 14-15 -0.2063827203092501 1106 16-17 -0.1476478341910905 1106 18-19 -0.1411565108042936 1106 20-21 0.05974022781298771 1106 22-23 -0.0249688703351012 1106 24-25 -0.04670699327409977 1106 26-27 -0.14924102534813954 1106 28-29 -0.06062413719318016 1106 30-31 0.08354905864161566 1106 32-33 0.010167519485875687 1106 34-35 -0.12692813945874093 1106 36-37 -0.14100783846902232 1106 38-39 -0.04946868411485639 1106 40-41 0.03423056968600946 1106 42-43 0.09266643137305408 1106 44-45 0.1386679333053209 1106 46-47 0.14817991263426222 1106 48-49 0.051737169651055126 1106 50-51 0.001405015298928447 1106 52-53 -0.13547044693276433 1106 54-55 -0.3251449336134655 1106 56-57 -0.2481042065003578 1106 58-59 0.04023260451155153 1106 60-61 -0.02478505583871282 1106 62-63 -0.04592310445286785 1106 64-65 -0.1976988077415669 1106 66-67 -0.134562869730253 1106 68-69 -0.06304735267043071 1106 70-71 -0.18939977763540838 1106 72-73 0.03164568085812647 1106 74-75 0.025523849602016924 1106 76-77 -0.02794317621544451 1106 78-79 0.0010114264669631723 1106 80-81 -0.22427932436831455 1106 82-83 -0.28621990004429776 1106 84-85 -0.31226477333428093 1106 86-87 -0.0766139251286937 1106 88-89 0.007212483529574598 1106 90-91 -0.11292664583390177 1106 92-93 -0.07412150455851574 1106 94-95 -0.25901794489103125 1106 96-97 -0.12011065085180306 1106 98-99 0.1590610051520187 1106 100-101 0.2974267703246305 1107 1 0.02693192416761292 1107 2 0.11104936101286 1107 3 0.05268088475128252 1107 4 0.09445798594617116 1107 5 -3.054164679525684E-4 1107 6 0.05467145232728399 1107 7 0.008390470130869687 1107 8 -0.027520425716744512 1107 9 -0.0490261304277837 1107 10-11 0.027113368154076056 1107 12-13 0.04519468152709294 1107 14-15 0.10741449938853975 1107 16-17 0.07362544564649909 1107 18-19 0.01735401107954715 1107 20-21 0.05802806128844651 1107 22-23 0.0170679539229468 1107 24-25 -0.003336000284242857 1107 26-27 0.05727243599955756 1107 28-29 0.11851445488014889 1107 30-31 0.1250555294211253 1107 32-33 0.11451793044587788 1107 34-35 0.14217559541300773 1107 36-37 0.13121373874372466 1107 38-39 0.07290106734297552 1107 40-41 0.09166377005843174 1107 42-43 0.20142325790865812 1107 44-45 0.22549388172502915 1107 46-47 0.12512293694577892 1107 48-49 0.10827603102695349 1107 50-51 0.10310710633067544 1107 52-53 0.20119369307610668 1107 54-55 0.15124860935238615 1107 56-57 0.004538553001403045 1107 58-59 0.13061689225872186 1107 60-61 0.27273245839264604 1107 62-63 0.2170500377975273 1107 64-65 0.09321169652684347 1107 66-67 0.1506868979551257 1107 68-69 0.2570488293628088 1107 70-71 0.31336453278941434 1107 72-73 0.158044739767071 1107 74-75 0.1576498141691971 1107 76-77 0.016591057995945846 1107 78-79 0.17259718003153068 1107 80-81 0.36874182882855067 1107 82-83 0.31658549081142695 1107 84-85 0.42245517467350524 1107 86-87 0.5213313193499154 1107 88-89 0.34955170602180985 1107 90-91 0.41228555116177645 1107 92-93 0.5659767770917661 1107 94-95 0.46813470749912867 1107 96-97 0.6050835081203445 1107 98-99 0.6421944522739196 1107 100-101 0.5256407969230423 1108 1 0.036357906175375376 1108 2 0.01580413483965515 1108 3 0.08054412232952757 1108 4 0.02810151495681623 1108 5 -0.04429615516598062 1108 6 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100-101 -0.7781734502489321 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 1.0 4 13.0 5 40.0 6 56.0 7 174.0 8 391.0 9 807.0 10 1354.0 11 2270.0 12 3620.0 13 5435.0 14 7119.0 15 9876.0 16 13656.0 17 20249.0 18 19923.0 19 12129.0 20 9513.0 21 9389.0 22 9804.0 23 10645.0 24 11624.0 25 13954.0 26 16358.0 27 19557.0 28 24126.0 29 29221.0 30 36590.0 31 45507.0 32 57553.0 33 75209.0 34 106690.0 35 163053.0 36 273728.0 37 476937.0 38 470583.0 39 57602.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.22607671473022 19.332695706641427 14.923392974756833 22.517834603871524 2 15.530162001804984 20.6791824156011 40.0570982644153 23.733557318178615 3 18.887984394156742 25.057901450886767 30.33063143108164 25.72348272387486 4 12.834760217454857 17.244708186521194 36.957498226838595 32.963033369185354 5 13.449479392603195 37.740785263966664 33.89854484053203 14.911190502898108 6 31.301463894991073 36.47209943407708 17.328349452689697 14.898087218242154 7 28.039079534416704 33.72778833043887 20.43644835645681 17.796683778687616 8 26.392492173970876 34.764147033554025 19.74710522540999 19.096255567065114 9 26.8340549257305 16.59222427320019 20.269938260544574 36.303782540524736 10-11 25.076894900293485 26.8132281563638 26.838938376374788 21.270938566967924 12-13 25.902053988484003 24.35316213371002 27.83754218238076 21.90724169542522 14-15 22.773968948208797 25.552906222717013 26.42556469420969 25.2475601348645 16-17 21.622031435544972 28.87483977600282 27.145376056423665 22.357752732028544 18-19 21.55863989958124 26.937039244814404 30.23901570556934 21.265305150035015 20-21 22.47959189244591 26.111011568109998 30.210575635943282 21.19882090350081 22-23 22.146128353558208 25.95496343738693 30.192037475375518 21.706870733679345 24-25 21.906286451740996 26.286228377137466 30.176780085871 21.63070508525054 26-27 21.644713032331985 26.562151712661485 30.166443183583947 21.62669207142258 28-29 21.409427019171495 26.645603798364277 30.407338435011184 21.537630747453047 30-31 21.621823509762685 26.3899773819551 30.236409940027688 21.75178916825453 32-33 21.505133511502535 26.482074836524504 30.09269381360431 21.92009783836865 34-35 22.05056451696976 26.585390917390338 29.83381462357841 21.530229942061492 36-37 22.106306809368697 26.28496975337366 29.882996742671008 21.72572669458663 38-39 21.700822698485783 26.485852539766658 29.9425513457431 21.870773416004457 40-41 21.91869122018768 26.361428008001162 29.89826288132804 21.821617890483118 42-43 21.979883386482747 26.331856367766658 29.59984433661512 22.08841590913547 44-45 21.759322222343553 26.680571121684498 29.560575211619383 21.999531444352563 46-47 22.00789869577712 26.95547813812344 28.975616486901934 22.061006679197504 48-49 22.110689942191712 26.781710801717125 28.931244022249764 22.1763552338414 50-51 22.104163119930234 27.13048631526388 28.693821556183035 22.07152900862285 52-53 22.266117652547717 27.191486045753273 28.385075564719024 22.157320736979983 54-55 21.891557468545926 27.129046940012298 28.532189642156425 22.44720594928535 56-57 21.96660351614557 27.40912951051811 28.465531677919437 22.158735295416882 58-59 22.03256660957145 27.025784174118744 28.526829210185017 22.41482000612479 60-61 22.011447500276706 27.122942003600425 28.72590969756741 22.139700798555463 62-63 21.822169654693877 27.209776038174216 28.57614022095899 22.391914086172914 64-65 21.617182765417073 27.084451124027986 28.541173329071455 22.75719278148348 66-67 21.678157679091782 27.241814545929223 28.388202483369014 22.691825291609984 68-69 21.65430745173677 27.11853554656799 28.503664334956273 22.723492666738967 70-71 21.66984404617317 27.2030258645806 28.26642637372237 22.86070371552386 72-73 21.88307011792454 27.09383187997794 28.111867251880245 22.91123075021727 74-75 21.77402503420998 27.474124748171374 27.85580735798702 22.89604285963163 76-77 21.70669701597657 27.223251568546775 28.1271792265393 22.942872188937358 78-79 21.772734559846494 27.286658530060357 28.14211894897812 22.79848796111503 80-81 21.671556406386262 27.571580379429246 27.925517790429943 22.831345423754556 82-83 21.774581811648225 27.518163435681238 27.854232906914067 22.853021845756473 84-85 21.74280989044588 27.273749300148108 27.798414860287725 23.185025949118284 86-87 21.753185436148456 27.135079169656066 27.954980145233506 23.15675524896197 88-89 21.686029991127988 27.18159088230002 27.865468014220042 23.266911112351952 90-91 21.630673078688893 27.276043711474884 27.945479281124225 23.147803928711998 92-93 21.715842511227766 26.90723135937565 27.99043756835493 23.386488561041652 94-95 21.488606848547448 27.04985648436069 27.920777778825602 23.540758888266264 96-97 21.555319795757562 26.917812893579267 28.01402150377526 23.51284580688791 98-99 21.484785059086352 26.93808155103106 27.770909034958454 23.806224354924126 100-101 21.455600485019826 26.769922814742976 28.02480986597431 23.74966683426289 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1156.0 1 770.0 2 565.5 3 772.0 4 636.0 5 624.5 6 1280.5 7 2660.5 8 4216.5 9 4906.5 10 4190.0 11 3393.0 12 3383.0 13 3533.5 14 3763.5 15 3851.0 16 3864.0 17 3885.5 18 4039.0 19 4306.0 20 4573.5 21 5171.5 22 6049.0 23 7152.0 24 8418.0 25 10245.5 26 12466.0 27 15093.5 28 17814.0 29 20277.5 30 23493.5 31 27539.0 32 31137.5 33 33778.0 34 36177.5 35 38486.0 36 42250.0 37 46670.0 38 49710.0 39 51855.0 40 54115.0 41 59467.0 42 64508.0 43 67847.0 44 71860.0 45 75415.5 46 76259.0 47 77391.5 48 82588.0 49 82600.0 50 78578.0 51 75723.0 52 72938.0 53 71502.5 54 69426.0 55 63417.5 56 54945.5 57 48510.5 58 43963.5 59 38181.0 60 31186.5 61 25347.5 62 20436.5 63 15488.0 64 11702.5 65 8877.5 66 6608.5 67 5546.5 68 4305.0 69 2915.5 70 2196.0 71 1775.5 72 1493.0 73 1249.5 74 980.0 75 822.0 76 518.5 77 243.5 78 125.5 79 63.0 80 26.5 81 14.0 82 8.0 83 6.0 84 3.5 85 2.0 86 2.5 87 2.0 88 0.5 89 1.5 90 1.0 91 1.0 92 1.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0169250676134116 3 0.006601272705524173 4 0.0 5 4.963362936484341E-5 6 4.963362936484341E-5 7 0.0 8 5.956035523781209E-4 9 2.9780177618906044E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.729849615066388E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005608600118227306 26-27 0.021938064179260787 28-29 4.963362936484341E-5 30-31 0.0 32-33 5.707867376956991E-4 34-35 0.019158580934829555 36-37 0.0037473390170456775 38-39 0.01764475523920183 40-41 0.015212707400324505 42-43 0.007916563883692523 44-45 0.003027651391255448 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.2335133214179535E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 4.963362936484341E-5 84-85 0.0025313150976070137 86-87 3.722522202363256E-4 88-89 2.4816814682421704E-5 90-91 2.9780177618906044E-4 92-93 1.7371770277695194E-4 94-95 0.0 96-97 2.4816814682421704E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2014763.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.02297711368676 #Duplication Level Percentage of deduplicated Percentage of total 1 70.19190308872618 33.0063225250729 2 15.09101155802994 14.19248582231248 3 5.87696101963065 8.290566105723636 4 2.877833776566597 5.4129724724994315 5 1.6268950989004647 3.8250725500982843 6 1.0030391314754903 2.829953167410253 7 0.6543990580710545 2.1540254351625348 8 0.44634696821533315 1.6790850616922464 9 0.34217736386059694 1.4481178514674664 >10 1.7042354384524478 14.411219792263887 >50 0.11444130162831304 3.7203458020309577 >100 0.06600511077877527 5.78278967723153 >500 0.003272970124150924 1.0360771791534356 >1k 0.0011613764956664567 0.7539845761561753 >5k 1.0557968142422332E-4 0.33471811499604487 >10k+ 2.1115936284844664E-4 1.1222638667288036 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12571 0.6239443547454464 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10034 0.4980238370468387 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6742 0.3346299291777743 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2217 0.11003775630185784 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 2.4816814682421704E-5 0.0 18-19 0.0 0.0 0.0 9.926725872968681E-5 0.0 20-21 0.0 0.0 0.0 2.2335133214179535E-4 0.0 22-23 0.0 0.0 0.0 4.467026642835907E-4 0.0 24-25 0.0 0.0 0.0 0.0011663902900738202 0.0 26-27 0.0 0.0 0.0 0.0026553991710191226 0.0 28-29 0.0 0.0 0.0 0.007693212551550728 0.0 30-31 0.0 0.0 0.0 0.02211178188203774 0.0 32-33 0.0 0.0 0.0 0.053157617049747294 0.0 34-35 0.0 0.0 0.0 0.09738118081382277 0.0 36-37 0.0 0.0 0.0 0.16927549294879846 0.0 38-39 0.0 0.0 0.0 0.27707973592923835 0.0 40-41 0.0 0.0 0.0 0.3967960499572406 0.0 42-43 0.0 0.0 0.0 0.5176291206459519 0.0 44-45 0.0 0.0 0.0 0.6533770969587986 0.0 46-47 0.0 0.0 0.0 0.8130981162548647 0.0 48-49 0.0 0.0 0.0 0.9803386303997046 0.0 50-51 0.0 0.0 0.0 1.1530636605893596 0.0 52-53 0.0 0.0 0.0 1.3207260605838007 0.0 54-55 0.0 0.0 0.0 1.500300531625804 0.0 56-57 0.0 0.0 0.0 1.7068260634129175 0.0 58-59 0.0 0.0 0.0 1.9259089034293364 0.0 60-61 0.0 0.0 0.0 2.1586906251504523 0.0 62-63 0.0 0.0 0.0 2.3850447918688205 0.0 64-65 0.0 0.0 0.0 2.6202833782434958 0.0 66-67 0.0 0.0 0.0 2.8711565578680966 0.0 68-69 0.0 0.0 0.0 3.150122371713199 0.0 70-71 0.0 0.0 0.0 3.4451694814725107 0.0 72-73 0.0 0.0 0.0 3.7413581647072136 0.0 74-75 0.0 0.0 0.0 4.03727386298041 0.0 76-77 0.0 0.0 0.0 4.350635781975349 0.0 78-79 0.0 0.0 0.0 4.6902042572749245 0.0 80-81 0.0 0.0 0.0 5.057592381833496 0.0 82-83 0.0 0.0 0.0 5.433889742863057 0.0 84-85 0.0 0.0 0.0 5.808698095011671 0.0 86-87 0.0 0.0 0.0 6.19338353940389 0.0 88-89 0.0 0.0 0.0 6.601893125891234 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7015 0.0 43.884136 1 GTATCAA 11045 0.0 38.410145 1 TAGCGCG 140 1.708031E-8 30.534336 4 TATCAAC 14060 0.0 30.140505 2 ATCAACG 14160 0.0 29.821814 3 TCAACGC 14395 0.0 29.366508 4 CAACGCA 14435 0.0 29.285131 5 AACGCAG 14750 0.0 28.691925 6 GTACATG 9655 0.0 25.734138 1 ACGCAGA 16740 0.0 25.195993 7 CGCAGAG 16680 0.0 25.172722 8 TACATGG 9750 0.0 24.948036 2 GAGTACT 9930 0.0 23.940258 12-13 ACATGGG 9920 0.0 23.702223 3 GCAGAGT 17740 0.0 23.346731 9 GTACTTT 10605 0.0 22.214933 14-15 CGCGTGC 195 4.2275497E-7 21.921541 9 AGTACTT 10235 0.0 21.347351 12-13 CATGGGG 4910 0.0 20.895185 4 CGCGCGT 205 6.837454E-7 20.852716 7 >>END_MODULE