FastQCFastQC Report
Thu 26 May 2016
SRR938104_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938104_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1989878
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124860.6274756542863431No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94590.47535577558021147No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62360.3133860467827676No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21610.1085996226904363No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67400.044.2361341
GTATCAA109400.039.051511
TATCAAC137650.030.7198312
ATCAACG140250.030.117183
TCAACGC143250.029.4864544
CAACGCA143550.029.4248315
AACGCAG145950.028.9344086
AATACCG1257.3141327E-626.6374245
ACGCAGA164650.025.5576927
CGCAGAG164200.025.5106168
GTACATG97450.024.4860441
GAGTACT93700.024.32834212-13
TACATGG98100.024.0494922
GCAGAGT173000.023.7995179
GTACTTT100750.022.64784414-15
ACATGGG101200.022.3263283
AGTACTT97800.021.7286212-13
AGAGTAC155950.020.51602410-11
CAGAGTA168550.020.43492910-11
CCGACGC3350.019.85340994-95
Produced by FastQC (version 0.11.5)