##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938104_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1989878 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.08091149306641 33.0 31.0 34.0 30.0 34.0 2 32.193136463642496 34.0 31.0 34.0 30.0 34.0 3 32.21599565400492 34.0 31.0 34.0 30.0 34.0 4 35.7264797138317 37.0 35.0 37.0 35.0 37.0 5 35.73540438157515 37.0 35.0 37.0 35.0 37.0 6 35.773011712275824 37.0 36.0 37.0 35.0 37.0 7 35.710768700392684 37.0 36.0 37.0 35.0 37.0 8 35.71133255405608 37.0 36.0 37.0 35.0 37.0 9 37.36489121443626 39.0 37.0 39.0 35.0 39.0 10-11 37.284201594268595 39.0 37.0 39.0 34.0 39.0 12-13 37.14649164421135 39.0 37.0 39.0 34.0 39.0 14-15 38.35472853109587 40.0 38.0 41.0 34.0 41.0 16-17 38.207094354528266 40.0 38.0 41.0 33.0 41.0 18-19 38.39398018370976 40.0 38.0 41.0 34.0 41.0 20-21 38.41176117329806 40.0 38.0 41.0 34.0 41.0 22-23 38.303689723691605 40.0 38.0 41.0 34.0 41.0 24-25 38.19674296615169 40.0 38.0 41.0 34.0 41.0 26-27 38.03323972625458 40.0 38.0 41.0 33.0 41.0 28-29 37.86040928137303 40.0 38.0 41.0 33.0 41.0 30-31 37.64997602868115 40.0 37.5 41.0 33.0 41.0 32-33 37.364021563131004 40.0 37.0 41.0 32.0 41.0 34-35 37.131996785732596 40.0 36.0 41.0 31.0 41.0 36-37 36.89292182736831 39.5 36.0 41.0 31.0 41.0 38-39 36.622645709938 39.0 35.5 41.0 30.0 41.0 40-41 36.51250428418224 39.0 35.0 41.0 30.0 41.0 42-43 36.29472912409706 39.0 35.0 41.0 30.0 41.0 44-45 35.9429532865834 39.0 35.0 41.0 28.5 41.0 46-47 35.52144277186843 39.0 35.0 40.5 26.5 41.0 48-49 35.103353572430066 39.0 35.0 40.0 25.0 41.0 50-51 34.37270877913119 38.5 34.0 39.5 24.0 40.5 52-53 34.439679216514776 38.0 34.0 40.0 24.0 40.5 54-55 34.54592191079051 38.0 34.0 40.0 23.5 41.0 56-57 34.27422711342102 38.0 34.0 40.0 23.0 41.0 58-59 33.91440404889144 38.0 34.0 40.0 21.5 41.0 60-61 33.46178710453606 37.5 33.0 40.0 19.0 41.0 62-63 32.95906357073147 37.0 32.5 40.0 17.0 41.0 64-65 32.474049162813 36.0 32.0 39.0 12.0 41.0 66-67 31.950407763692045 35.5 31.0 39.0 7.5 40.0 68-69 31.323674868509528 35.0 30.5 38.5 4.5 40.0 70-71 30.780186021454583 35.0 30.0 38.0 2.0 40.0 72-73 30.12091042767446 34.5 29.0 37.0 2.0 39.0 74-75 29.479797002630313 34.0 29.0 36.5 2.0 39.0 76-77 28.774604272221715 34.0 27.5 36.0 2.0 38.5 78-79 28.041709089702987 34.0 26.0 35.0 2.0 37.5 80-81 27.342176254021602 33.0 25.5 35.0 2.0 37.0 82-83 26.638628599341267 32.5 24.5 35.0 2.0 36.5 84-85 25.933556479341952 32.0 22.0 35.0 2.0 36.0 86-87 25.205545767127433 31.5 19.0 34.5 2.0 35.5 88-89 24.364099457353667 31.0 13.5 34.0 2.0 35.0 90-91 23.485509161868215 30.5 4.5 34.0 2.0 35.0 92-93 22.562755103579214 30.0 2.0 34.0 2.0 35.0 94-95 21.490580829578498 29.0 2.0 34.0 2.0 35.0 96-97 20.216791933977863 28.0 2.0 34.0 2.0 35.0 98-99 18.648651073080863 25.5 2.0 33.5 2.0 35.0 100-101 14.558722946833925 8.5 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2804344508527592 1101 2 0.23502274708616966 1101 3 0.23952636193665455 1101 4 0.05469773133715705 1101 5 0.14267769364248295 1101 6 0.19167270996122454 1101 7 0.2164356182573428 1101 8 0.2559607705985343 1101 9 0.26163073783497026 1101 10-11 0.3629610215266226 1101 12-13 0.28688471057223097 1101 14-15 0.46461469437264213 1101 16-17 0.4295898483134053 1101 18-19 0.46869822748966783 1101 20-21 0.45020271232827724 1101 22-23 0.38155862359802484 1101 24-25 0.2639561182446002 1101 26-27 0.353854279620613 1101 28-29 0.5388907455852632 1101 30-31 0.4521479443842793 1101 32-33 0.5060769547591164 1101 34-35 0.5393344333328187 1101 36-37 0.40607850729583106 1101 38-39 0.3616219508304539 1101 40-41 0.5306694666693659 1101 42-43 0.5327691022522245 1101 44-45 0.6506495410303401 1101 46-47 0.7043185360631128 1101 48-49 0.8122892535476396 1101 50-51 0.792031720391968 1101 52-53 0.6967958068004521 1101 54-55 0.6846539778258958 1101 56-57 0.6349907923029434 1101 58-59 0.6853587051337087 1101 60-61 0.6418777691695539 1101 62-63 0.4347999589264404 1101 64-65 0.4005585060454351 1101 66-67 0.4705490813408133 1101 68-69 0.5888560148603901 1101 70-71 0.5892573314427203 1101 72-73 0.5247500274986621 1101 74-75 0.464475760486156 1101 76-77 0.23547867952657242 1101 78-79 -0.10499985814748669 1101 80-81 0.016797963271383054 1101 82-83 -0.03600463820939481 1101 84-85 -0.06227515798371641 1101 86-87 0.0706364398869681 1101 88-89 0.18521744407486196 1101 90-91 0.02543903126543512 1101 92-93 -0.03252230529680489 1101 94-95 -0.28257165595231726 1101 96-97 -0.2550534304227945 1101 98-99 -0.4522350608621224 1101 100-101 -0.7123876112197749 1102 1 0.18683814292599976 1102 2 0.1728509121326951 1102 3 0.14759974160369893 1102 4 0.06477415886953963 1102 5 0.11049154295786678 1102 6 0.15353773801544435 1102 7 0.23097820755748444 1102 8 0.2517520610924251 1102 9 0.19286673958980316 1102 10-11 0.2785651684706707 1102 12-13 0.3840703417343505 1102 14-15 0.3695966265345447 1102 16-17 0.35350750976945733 1102 18-19 0.403614613229351 1102 20-21 0.32764591120149333 1102 22-23 0.31893393819776605 1102 24-25 0.27755187778650026 1102 26-27 0.3475924460525306 1102 28-29 0.18748962226216292 1102 30-31 0.2411114104075054 1102 32-33 0.32377376719994544 1102 34-35 0.29032859736339134 1102 36-37 0.29279576006084085 1102 38-39 0.15463596062056695 1102 40-41 0.20019629712031417 1102 42-43 0.3205575815521442 1102 44-45 0.3784148438542374 1102 46-47 0.3827162132202844 1102 48-49 0.3529335191737246 1102 50-51 0.3260587124721397 1102 52-53 0.33465918392761296 1102 54-55 0.27271563937430443 1102 56-57 0.533404661244731 1102 58-59 0.3587950867172154 1102 60-61 0.4566499585528163 1102 62-63 0.4551967009431124 1102 64-65 0.23641941255447563 1102 66-67 0.18019430519728985 1102 68-69 0.153919569779692 1102 70-71 -0.21868674484626993 1102 72-73 -0.11768370452008625 1102 74-75 -0.16570272435533084 1102 76-77 -0.22526735478500015 1102 78-79 -0.15827645769954302 1102 80-81 -0.14183938693296128 1102 82-83 -0.18704945568382314 1102 84-85 -0.2546614114183363 1102 86-87 -0.26061226130677895 1102 88-89 -0.10992502053988673 1102 90-91 -0.2710085893215073 1102 92-93 -0.4869340615137574 1102 94-95 -0.7872272991211737 1102 96-97 -0.9436496229001072 1102 98-99 -0.7090745745890636 1102 100-101 -0.9413541655156497 1103 1 0.27673896878158644 1103 2 0.2391188211148787 1103 3 0.36577094699936197 1103 4 0.1517606239909881 1103 5 0.17860225874647284 1103 6 0.043594587441894816 1103 7 0.2191839115598242 1103 8 0.18028262766686964 1103 9 0.3142060933497035 1103 10-11 0.3160716336696865 1103 12-13 0.24279379632669418 1103 14-15 0.3087356601025988 1103 16-17 0.3379282847290028 1103 18-19 0.15396180168848161 1103 20-21 0.22159171461899518 1103 22-23 0.22235861679391888 1103 24-25 0.2857957510494842 1103 26-27 0.24375116900846905 1103 28-29 0.15246451914448755 1103 30-31 0.1392389890419139 1103 32-33 0.3758489887343046 1103 34-35 0.1817431732117072 1103 36-37 0.12113741954549795 1103 38-39 0.11432899421799192 1103 40-41 0.25577124481002755 1103 42-43 0.2781347947971895 1103 44-45 0.479202136798186 1103 46-47 0.2641328972706205 1103 48-49 0.3308811997498111 1103 50-51 0.3856749809213298 1103 52-53 0.4059943960817094 1103 54-55 0.329607312973657 1103 56-57 0.39907742562947135 1103 58-59 0.42359085568597976 1103 60-61 0.4233815057597923 1103 62-63 0.44487315980644127 1103 64-65 0.280856072442198 1103 66-67 0.2656767717991855 1103 68-69 0.3918807251187175 1103 70-71 0.23073178201086364 1103 72-73 0.350681907020558 1103 74-75 0.3907509640424358 1103 76-77 0.5129831588987805 1103 78-79 0.29933676950895105 1103 80-81 0.284288494218746 1103 82-83 0.1873198497813533 1103 84-85 -0.08689908917148514 1103 86-87 -0.38533983645060843 1103 88-89 -0.01985105062927417 1103 90-91 0.20313629374530606 1103 92-93 0.033962209683576106 1103 94-95 -0.27165375320572593 1103 96-97 -0.5955897643684978 1103 98-99 -0.7549053904078349 1103 100-101 -0.6902671671382166 1104 1 0.14243265121930904 1104 2 0.15314936178796046 1104 3 0.15964140598902787 1104 4 0.14251984364312165 1104 5 0.11686785859048143 1104 6 0.05128446388619068 1104 7 0.10528837953540915 1104 8 0.037182202797616526 1104 9 0.09685276623164185 1104 10-11 0.1589223349278157 1104 12-13 0.06418365949797078 1104 14-15 0.07266087760402229 1104 16-17 0.18363295119061007 1104 18-19 0.24845580140712542 1104 20-21 0.277108060441428 1104 22-23 0.2940691122592298 1104 24-25 0.328101595975518 1104 26-27 0.18022650012539287 1104 28-29 0.10538899563494653 1104 30-31 0.22175415035950152 1104 32-33 0.35481707333619283 1104 34-35 0.2827910268364704 1104 36-37 0.29463399783395516 1104 38-39 0.32009637505815647 1104 40-41 0.28824034793772313 1104 42-43 0.28503782727265303 1104 44-45 0.22157193597811897 1104 46-47 0.01958947474308559 1104 48-49 0.20517876930271228 1104 50-51 0.19437731625833266 1104 52-53 0.21471872066094022 1104 54-55 0.24126155836777485 1104 56-57 0.1490283472304057 1104 58-59 0.17227699846291955 1104 60-61 0.01650859589686604 1104 62-63 -0.09657576699932235 1104 64-65 -0.06654242799745447 1104 66-67 -0.027528181449824274 1104 68-69 -0.07237099289017834 1104 70-71 -0.027337938723110966 1104 72-73 -0.22009921179738257 1104 74-75 -0.29650862796969335 1104 76-77 -0.374096916118976 1104 78-79 -0.42443079307362197 1104 80-81 -0.5063825300269151 1104 82-83 -0.6267878651840206 1104 84-85 -0.23763825887359857 1104 86-87 -0.11205726912444547 1104 88-89 -0.1897240402363103 1104 90-91 -0.5604586877267685 1104 92-93 -0.5667894514977014 1104 94-95 -0.9584475971716522 1104 96-97 -0.7453428622685792 1104 98-99 -0.5766060172072116 1104 100-101 -0.8479549373840154 1105 1 0.1279154722468192 1105 2 0.21317147034358186 1105 3 0.23741283536660518 1105 4 0.21279814541583164 1105 5 0.2592365273208799 1105 6 0.20153295840842844 1105 7 0.19101722626285778 1105 8 0.13314331028107063 1105 9 0.24551617606823584 1105 10-11 0.28939304361080787 1105 12-13 0.28352894797733796 1105 14-15 0.2743778931304064 1105 16-17 0.3531341029717936 1105 18-19 0.32204320678572884 1105 20-21 0.2591981402095769 1105 22-23 0.42871830268705224 1105 24-25 0.32921017276496656 1105 26-27 0.40979372061439534 1105 28-29 0.3420394688426853 1105 30-31 0.29369815142361233 1105 32-33 0.3234768857460111 1105 34-35 0.45883279427139456 1105 36-37 0.4586427757568359 1105 38-39 0.21157450431561386 1105 40-41 0.32016222029255914 1105 42-43 0.3912835950058451 1105 44-45 0.28832423047133915 1105 46-47 0.3730491261252311 1105 48-49 0.5406234495448814 1105 50-51 0.5437484215651907 1105 52-53 0.48910300141743335 1105 54-55 0.4987844126085079 1105 56-57 0.40118730714075923 1105 58-59 0.4948156582047929 1105 60-61 0.5046387250011577 1105 62-63 0.5922167118594999 1105 64-65 0.3822398379985046 1105 66-67 0.6196885740944467 1105 68-69 0.48857780575065846 1105 70-71 0.4622947710562464 1105 72-73 0.25710423729852394 1105 74-75 0.3127051428187997 1105 76-77 0.20118994591718575 1105 78-79 0.12185055593118932 1105 80-81 0.2605954097862231 1105 82-83 0.29270912990654807 1105 84-85 0.29796681924195667 1105 86-87 0.11714265106709476 1105 88-89 0.24801074980294757 1105 90-91 0.13551063236964467 1105 92-93 -0.028042922964164774 1105 94-95 0.03232870636652052 1105 96-97 0.09628369863172637 1105 98-99 0.05637432492117611 1105 100-101 -0.18149346008105915 1106 1 0.17185722444828855 1106 2 0.09660926831318761 1106 3 0.14433764887269263 1106 4 -0.0022132124842144663 1106 5 0.20774913318773258 1106 6 0.13222873495226395 1106 7 0.19295402866344347 1106 8 0.07483591783056909 1106 9 0.2915226823220891 1106 10-11 0.20268038445998826 1106 12-13 0.2628814074534702 1106 14-15 0.29654420223354805 1106 16-17 -0.1162390040448642 1106 18-19 0.1909357063650674 1106 20-21 0.4158048273485093 1106 22-23 0.3657529442822067 1106 24-25 0.28410546274196946 1106 26-27 0.22514371220113816 1106 28-29 0.07968074148837445 1106 30-31 0.07315507553601464 1106 32-33 -0.0450116021108542 1106 34-35 -0.08453668102166745 1106 36-37 0.041891100116032476 1106 38-39 0.12351221815262647 1106 40-41 0.08083654302518539 1106 42-43 -0.006868642921126877 1106 44-45 0.18946054630509224 1106 46-47 0.29093834453343703 1106 48-49 0.20939518379806543 1106 50-51 0.2425502332203635 1106 52-53 0.11825967733868481 1106 54-55 -0.12378625876957727 1106 56-57 -0.1398098043068785 1106 58-59 -0.16785383391478348 1106 60-61 0.07038221173625914 1106 62-63 -0.03042319363155599 1106 64-65 -0.2896448021944451 1106 66-67 -0.1599970855050863 1106 68-69 -0.10546402918119213 1106 70-71 -0.1928264499881216 1106 72-73 -0.33385536503475066 1106 74-75 -0.26534448840000024 1106 76-77 -0.5980390139235219 1106 78-79 -0.6199570328286974 1106 80-81 -0.18726692124748823 1106 82-83 -0.20099670048214335 1106 84-85 -0.18017185230440091 1106 86-87 -0.2851478587764227 1106 88-89 -0.5921408290853769 1106 90-91 -0.7575691113063883 1106 92-93 -0.6022645212029865 1106 94-95 -0.8259420533507935 1106 96-97 -0.8530965246000868 1106 98-99 -1.0888350250143262 1106 100-101 -0.9466241810973788 1107 1 0.045243667600239235 1107 2 -0.0016068326172700154 1107 3 0.04654505296134204 1107 4 0.19120458203027368 1107 5 -0.043903599237218316 1107 6 0.05141231320449435 1107 7 0.045586273147385725 1107 8 0.11159473635512995 1107 9 -0.06236781356031429 1107 10-11 -0.018408312316410047 1107 12-13 0.12847048004288553 1107 14-15 0.04275459538465043 1107 16-17 0.06459477759332799 1107 18-19 0.07878577250706797 1107 20-21 0.11389630225242797 1107 22-23 0.09158140894297162 1107 24-25 0.11099465213591486 1107 26-27 0.08298569522932553 1107 28-29 0.02000468739964134 1107 30-31 0.1311342480279194 1107 32-33 0.037539718576191206 1107 34-35 -0.051152685150974264 1107 36-37 -0.007204141638837314 1107 38-39 -0.11693807069742235 1107 40-41 0.04304321674481315 1107 42-43 -0.0336113224706267 1107 44-45 -0.13241662197792436 1107 46-47 -0.08012958578063234 1107 48-49 -0.08443554173634737 1107 50-51 -0.17309664208111286 1107 52-53 -0.15549530238812537 1107 54-55 -0.24488171418464333 1107 56-57 -0.270845500274973 1107 58-59 -0.34204670802042614 1107 60-61 -0.17194823227904976 1107 62-63 -0.278541539574654 1107 64-65 -0.32512002442556565 1107 66-67 -0.3825649158022131 1107 68-69 -0.4784716615374123 1107 70-71 -0.4903907783293242 1107 72-73 -0.34208673285785807 1107 74-75 -0.4976754175111502 1107 76-77 -0.772401126521352 1107 78-79 -0.522487107408093 1107 80-81 -0.6364820198282253 1107 82-83 -0.5765595342921266 1107 84-85 -0.7211542749384243 1107 86-87 -0.8768788006417729 1107 88-89 -0.9877353957939619 1107 90-91 -0.8897562285780367 1107 92-93 -0.9512184856078534 1107 94-95 -1.0242832981067167 1107 96-97 -1.137689035919447 1107 98-99 -1.620695465666664 1107 100-101 -1.642380519092539 1108 1 -0.04935367102395105 1108 2 -0.02185709591461915 1108 3 -0.07709714343490504 1108 4 0.08889361617742964 1108 5 0.1275877630377522 1108 6 0.09607693234980275 1108 7 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-0.24756466674263677 2316 100-101 -0.10540254709715846 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6340.0 3 1488.0 4 1331.0 5 1447.0 6 1740.0 7 2206.0 8 2793.0 9 3493.0 10 4550.0 11 5917.0 12 7974.0 13 10609.0 14 12354.0 15 15882.0 16 19546.0 17 26174.0 18 24749.0 19 18621.0 20 18459.0 21 19603.0 22 21397.0 23 23770.0 24 26653.0 25 30531.0 26 34620.0 27 39951.0 28 46303.0 29 54948.0 30 65000.0 31 78164.0 32 96685.0 33 121315.0 34 158121.0 35 212402.0 36 276635.0 37 313319.0 38 173611.0 39 11177.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.95149804685731 18.901197451100977 14.555517867154089 22.59178663488763 2 15.342045864229531 20.41137720907711 40.51784432992438 23.728732596768978 3 18.823162865565806 25.139864170058722 30.1045074138706 25.93246555050487 4 12.609587434947006 16.916244050752155 37.336367378446134 33.13780113585471 5 13.49619263139979 37.561269881554125 33.85219493968939 15.090342547356697 6 31.172139018879253 36.46463369742944 17.502697863010496 14.860529420680813 7 27.970858141350703 33.77234735234877 20.62694665430943 17.629847851991094 8 26.354710399486613 34.54480714673324 19.856747488026667 19.243734965753475 9 27.01179934917147 16.62838214211853 20.388534669677096 35.9712838390329 10-11 25.09967234524438 26.762596949435117 26.90084280671287 21.23688789860763 12-13 26.028358424187488 24.3902027762475 27.769546844552135 21.811891955012882 14-15 22.918931378233 25.529061785357683 26.53109599961349 25.020910836795824 16-17 21.631941631141466 28.9099571382632 27.155101915517537 22.302999315077802 18-19 21.550188602144964 26.90693740361362 30.315891922956823 21.226982071284592 20-21 22.554422834252385 26.07394434861815 30.270632742900784 21.101000074228683 22-23 22.163774935984108 25.974388607311283 30.184028381008492 21.677808075696117 24-25 21.887535030089108 26.227589305321352 30.260809212686784 21.624066451902756 26-27 21.63170015343541 26.495554852593038 30.18515177069799 21.687593223273563 28-29 21.475199681968174 26.64696580277246 30.39098540802552 21.48684910723384 30-31 21.64115207788242 26.38016980436328 30.192869866793576 21.785808250960727 32-33 21.5627000990038 26.429711706475757 30.10299162556185 21.9045965689586 34-35 22.110095227459595 26.57882630854441 29.755330245187885 21.55574821880811 36-37 22.17199725359711 26.2128311339475 29.83692604077855 21.77824557167684 38-39 21.70626846729932 26.50265647536139 29.900275012388 21.890800044951288 40-41 21.943191806473802 26.356871752890843 29.86696783614557 21.832968604489785 42-43 21.955232001480432 26.3859946253862 29.524798250836763 22.1339751222966 44-45 21.790224239933334 26.695700591110594 29.470639915236024 22.04343525372005 46-47 22.042572812189977 26.960585403822744 28.941447047211334 22.055394736775945 48-49 22.155968007593874 26.83976948896214 28.87647488250212 22.127787620941874 50-51 22.138932055018106 27.14837636551556 28.610616596694378 22.102074982771956 52-53 22.294028139362684 27.190668336254774 28.315327954927156 22.199975569455386 54-55 21.921169076725942 27.185815532230535 28.461702645974324 22.431312745069203 56-57 21.94233664521026 27.43375198875989 28.456119096870513 22.167792269159328 58-59 22.096989873224874 26.89557437225232 28.529494505958635 22.477941248564175 60-61 22.087636964177538 26.934214462210583 28.790643074935335 22.187505498676547 62-63 21.898420015516763 27.038184005868814 28.65303846457467 22.410357514039752 64-65 21.776817403575947 26.878702006672068 28.643146604177545 22.701333985574447 66-67 21.77870706624842 27.100671083579908 28.485093609083318 22.635528241088355 68-69 21.780881678117712 26.944953263390808 28.60272939283267 22.671435665658805 70-71 21.799880279836454 26.984075106427003 28.359675372818195 22.85636924091835 72-73 21.922834606869703 26.88193344894633 28.223382821664515 22.971849122519444 74-75 21.83302496757132 27.246804894971294 28.04598336835966 22.87418676909773 76-77 21.858623644055637 26.993022389025622 28.12635952578484 23.0219944411339 78-79 21.957279667679366 27.084920910986405 28.089794732492745 22.86800468884148 80-81 21.894322631983606 27.371723674596733 27.879633755812577 22.854319937607087 82-83 21.99133206863168 27.24992233847156 27.83095121046425 22.92779438243251 84-85 22.00403375283525 26.98950093304096 27.82605854596405 23.18040676815974 86-87 22.04908615977997 26.873153161576262 27.86896617764484 23.20879450099893 88-89 22.022111700041236 26.769564605187725 27.840023333702113 23.368300361068925 90-91 21.95656543372054 26.889850903886853 27.808567121173894 23.345016541218712 92-93 22.137883547746874 26.521038479761234 27.860004003593176 23.48107396889872 94-95 22.004370063780602 26.572618173158435 27.74150587398635 23.681505889074614 96-97 22.126324857547406 26.43235233547828 27.746529552768877 23.694793254205436 98-99 22.12850680644279 26.34939639081533 27.476788903728256 24.04530789901363 100-101 22.809894356398324 25.349104975812132 27.498511689839685 24.342488977949863 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1033.0 1 718.0 2 549.5 3 712.5 4 599.5 5 612.5 6 1171.5 7 2426.5 8 3977.5 9 4583.0 10 3899.0 11 3279.0 12 3271.0 13 3453.0 14 3705.0 15 3879.5 16 3961.0 17 4050.5 18 4213.5 19 4424.5 20 4757.0 21 5342.0 22 6181.5 23 7298.0 24 8613.0 25 10406.0 26 12737.5 27 15325.0 28 17783.5 29 20331.5 30 23326.5 31 27093.0 32 30852.0 33 33485.0 34 35875.0 35 38247.5 36 41758.5 37 46025.0 38 49034.0 39 50774.5 40 52993.5 41 58119.5 42 62911.5 43 66064.0 44 69607.0 45 73185.5 46 74085.5 47 74656.5 48 78979.0 49 79694.5 50 76319.5 51 73588.5 52 71575.5 53 70044.5 54 67503.5 55 61968.5 56 54749.5 57 48886.0 58 44096.5 59 38406.0 60 32091.0 61 26473.5 62 21341.5 63 16586.0 64 12710.0 65 9893.0 66 7686.0 67 6293.5 68 4942.5 69 3497.5 70 2672.5 71 2132.0 72 1767.5 73 1479.0 74 1131.5 75 926.0 76 642.5 77 350.0 78 212.0 79 142.5 80 79.0 81 45.5 82 28.5 83 16.5 84 12.5 85 8.5 86 5.0 87 5.5 88 6.5 89 4.5 90 4.0 91 3.5 92 1.5 93 0.5 94 0.5 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1419182482544156 2 0.055028499234626445 3 0.15724582110059007 4 0.1332745022559172 5 0.2006153141046838 6 0.20337930265071527 7 0.17372924370237772 8 0.07543176013805872 9 0.12890237491946743 10-11 0.10628792317920999 12-13 0.15737145694359153 14-15 0.1442299477656419 16-17 0.14025985512679673 18-19 0.13862658916777815 20-21 0.13948091290018785 22-23 0.20317828530191298 24-25 0.22322976584494125 26-27 0.15320034695594403 28-29 0.1334503924361192 30-31 0.10357418897037908 32-33 0.12968131714607628 34-35 0.09817184772131758 36-37 0.09161365671664293 38-39 0.053194215926805566 40-41 0.05960164391987851 42-43 0.06382300824472656 44-45 0.04148495535907226 46-47 0.05512900790902758 48-49 0.045530429503718314 50-51 0.0560084588100376 52-53 0.028393700518323232 54-55 0.05158607713638726 56-57 0.028795735215927813 58-59 0.05233989219439584 60-61 0.03834405928403651 62-63 0.05331985176980699 64-65 0.0421382617426797 66-67 0.039273764522247095 68-69 0.0673659390173669 70-71 0.05394803098481415 72-73 0.05321934309540585 74-75 0.04512839480611374 76-77 0.022287798548453724 78-79 0.02382055583307117 80-81 0.027614758291714368 82-83 0.023921064507472316 84-85 0.020880677106837706 86-87 0.02505178709448519 88-89 0.06724030317436545 90-91 0.11395170960229722 92-93 0.08362321710175197 94-95 0.07927621693390248 96-97 0.07083348828420637 98-99 0.05899859187347164 100-101 0.13694306887155897 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1989878.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.297999040126186 #Duplication Level Percentage of deduplicated Percentage of total 1 72.13789158007552 35.5625370987129 2 14.321218994227875 14.120148804617653 3 5.468749509923223 8.087952242726566 4 2.6626262706353785 5.250485893359906 5 1.4831523660139672 3.655822195805871 6 0.8934201247596385 2.6426294671698045 7 0.6056100877216909 2.0898755866236254 8 0.4208560874106258 1.6597890394560235 9 0.2989974610161583 1.3265978891557264 >10 1.5381486716865969 13.585215435993547 >50 0.10584782794635218 3.6033553752038325 >100 0.059501689388338615 5.43662901894927 >500 0.0028569542935466576 0.9721125017088683 >1k 8.162726552990449E-4 0.5893237126162216 >5k 2.0406816382476122E-4 0.7894704395281325 >10k+ 1.0203408191238061E-4 0.6280552983719822 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12486 0.6274756542863431 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9459 0.47535577558021147 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6236 0.3133860467827676 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2161 0.1085996226904363 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.0050867440114419E-4 0.0 22-23 0.0 0.0 0.0 3.266531918037186E-4 0.0 24-25 0.0 0.0 0.0 6.281792150071512E-4 0.0 26-27 0.0 0.0 0.0 0.0019599191508223117 0.0 28-29 0.0 0.0 0.0 0.006909971365078663 0.0 30-31 0.0 0.0 0.0 0.02078016843243656 0.0 32-33 0.0 0.0 0.0 0.05037997304357353 0.0 34-35 0.0 0.0 0.0 0.09648832742509841 0.0 36-37 0.0 0.0 0.0 0.17199546906895802 0.0 38-39 0.0 0.0 0.0 0.28695226541526664 0.0 40-41 0.0 0.0 0.0 0.40982411987066547 0.0 42-43 0.0 0.0 0.0 0.5279218122920099 0.0 44-45 0.0 0.0 0.0 0.6723276502378538 0.0 46-47 0.0 0.0 0.0 0.8371618762557302 0.0 48-49 0.0 2.5127168600286047E-5 0.0 1.0066195012960595 0.0 50-51 0.0 5.0254337200572095E-5 0.0 1.1907765199675557 0.0 52-53 0.0 5.0254337200572095E-5 0.0 1.3629227520481155 0.0 54-55 0.0 5.0254337200572095E-5 0.0 1.5471048978882123 0.0 56-57 0.0 5.0254337200572095E-5 0.0 1.756338830822794 0.0 58-59 0.0 5.0254337200572095E-5 0.0 1.9754728681858889 0.0 60-61 0.0 5.0254337200572095E-5 0.0 2.2056879869017094 0.0 62-63 0.0 5.0254337200572095E-5 0.0 2.4362548859779345 0.0 64-65 0.0 5.0254337200572095E-5 0.0 2.6728774326868283 0.0 66-67 0.0 5.0254337200572095E-5 0.0 2.9306570553571625 0.0 68-69 0.0 5.0254337200572095E-5 0.0 3.213815118313786 0.0 70-71 0.0 5.0254337200572095E-5 0.0 3.5085819331637413 0.0 72-73 0.0 5.0254337200572095E-5 0.0 3.81322372527361 0.0 74-75 0.0 5.0254337200572095E-5 0.0 4.115905598232655 0.0 76-77 0.0 5.0254337200572095E-5 0.0 4.43429195156688 0.0 78-79 0.0 5.0254337200572095E-5 0.0 4.776699878082978 0.0 80-81 0.0 5.0254337200572095E-5 0.0 5.141144331461527 0.0 82-83 0.0 5.0254337200572095E-5 0.0 5.5083527733861075 0.0 84-85 0.0 5.0254337200572095E-5 0.0 5.8785262212055205 0.0 86-87 0.0 5.0254337200572095E-5 0.0 6.254202518948398 0.0 88-89 0.0 5.0254337200572095E-5 0.0 6.653724499692945 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6740 0.0 44.236134 1 GTATCAA 10940 0.0 39.05151 1 TATCAAC 13765 0.0 30.719831 2 ATCAACG 14025 0.0 30.11718 3 TCAACGC 14325 0.0 29.486454 4 CAACGCA 14355 0.0 29.424831 5 AACGCAG 14595 0.0 28.934408 6 AATACCG 125 7.3141327E-6 26.637424 5 ACGCAGA 16465 0.0 25.557692 7 CGCAGAG 16420 0.0 25.510616 8 GTACATG 9745 0.0 24.486044 1 GAGTACT 9370 0.0 24.328342 12-13 TACATGG 9810 0.0 24.049492 2 GCAGAGT 17300 0.0 23.799517 9 GTACTTT 10075 0.0 22.647844 14-15 ACATGGG 10120 0.0 22.326328 3 AGTACTT 9780 0.0 21.72862 12-13 AGAGTAC 15595 0.0 20.516024 10-11 CAGAGTA 16855 0.0 20.434929 10-11 CCGACGC 335 0.0 19.853409 94-95 >>END_MODULE