FastQCFastQC Report
Thu 26 May 2016
SRR938104_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938104_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1989878
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119010.5980768670240085No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95360.47922535954465545No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63900.32112521471165567No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22550.11332353038729008No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69750.042.3565981
GTATCAA108700.036.967131
TATCAAC137950.029.1625612
ATCAACG138750.029.0592293
TCAACGC141550.028.4829794
CAACGCA142300.028.2994845
AACGCAG145300.027.6825056
GTACATG95200.026.0440461
TACATGG97600.024.9163322
ACGCAGA163700.024.2524767
CGCAGAG164450.024.0263588
ACATGGG100150.023.5201513
GAGTACT94850.023.38356612-13
GCAGAGT173850.022.590689
GTACTTT101050.021.6193114-15
AGTACTT97550.020.88628612-13
AGAGTAC153300.020.21404510-11
CAGAGTA168900.019.78104610-11
TATCGTG1707.972526E-519.5540495
CATGGGG51000.019.5540474
Produced by FastQC (version 0.11.5)