##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938104_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1989878 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.547599903109635 34.0 31.0 34.0 31.0 34.0 2 32.75367233569093 34.0 31.0 34.0 31.0 34.0 3 32.80538907410404 34.0 31.0 34.0 31.0 34.0 4 36.261725593227325 37.0 35.0 37.0 35.0 37.0 5 36.23952473468223 37.0 37.0 37.0 35.0 37.0 6 36.27315443459348 37.0 37.0 37.0 35.0 37.0 7 36.22884468294036 37.0 37.0 37.0 35.0 37.0 8 36.219301886849344 37.0 37.0 37.0 35.0 37.0 9 37.90908739128731 39.0 38.0 39.0 35.0 39.0 10-11 37.89128856140929 39.0 38.0 39.0 35.0 39.0 12-13 37.81439917422073 39.0 38.0 39.0 35.0 39.0 14-15 39.095495050450324 40.0 38.5 41.0 36.0 41.0 16-17 38.99655531645659 40.0 38.0 41.0 36.0 41.0 18-19 39.252224256964496 40.5 39.0 41.0 36.0 41.0 20-21 39.24533162334575 41.0 39.0 41.0 36.0 41.0 22-23 39.15310712516043 40.5 39.0 41.0 36.0 41.0 24-25 39.0645434041685 40.0 39.0 41.0 35.0 41.0 26-27 38.89832416861738 40.0 38.0 41.0 35.0 41.0 28-29 38.78089108980551 40.0 38.0 41.0 35.0 41.0 30-31 38.54204478867548 40.0 38.0 41.0 34.5 41.0 32-33 38.33038231489569 40.0 38.0 41.0 34.0 41.0 34-35 38.174318475806054 40.0 38.0 41.0 34.0 41.0 36-37 38.16831685158588 40.0 38.0 41.0 34.0 41.0 38-39 38.03539211951687 40.0 38.0 41.0 33.5 41.0 40-41 37.866303361311594 40.0 37.0 41.0 33.0 41.0 42-43 37.628358874262645 40.0 37.0 41.0 33.0 41.0 44-45 37.32757410253292 40.0 37.0 41.0 32.5 41.0 46-47 36.89420657949884 40.0 36.5 41.0 31.0 41.0 48-49 36.47823459528675 39.5 36.0 41.0 30.5 41.0 50-51 36.11172292974745 39.0 35.5 41.0 29.5 41.0 52-53 35.640021147025095 39.0 35.0 40.0 28.0 41.0 54-55 35.36022057633684 38.5 35.0 40.0 27.5 41.0 56-57 35.04910175397688 38.0 34.5 40.0 27.0 41.0 58-59 34.64947097259229 38.0 34.0 40.0 26.0 41.0 60-61 34.21021540014011 37.5 34.0 40.0 25.0 41.0 62-63 33.78375232049402 37.0 33.0 40.0 23.5 41.0 64-65 33.32847591661398 36.0 33.0 39.0 22.5 40.5 66-67 32.81056577337907 36.0 32.0 39.0 21.5 40.0 68-69 32.27430701781717 35.0 31.5 38.5 19.5 40.0 70-71 31.713268853668417 35.0 31.0 38.0 17.5 40.0 72-73 31.162765506227018 35.0 30.5 37.0 13.5 39.0 74-75 30.593068017235225 34.0 30.0 36.5 8.0 39.0 76-77 29.214047795895027 32.5 28.5 35.0 7.5 37.5 78-79 29.91764344346739 34.0 29.5 36.0 7.0 37.5 80-81 29.61848841989308 34.0 29.0 35.0 2.0 37.0 82-83 29.101414760100873 34.0 29.0 35.0 2.0 37.0 84-85 28.550787786989957 34.0 28.5 35.0 2.0 36.0 86-87 27.952542567936327 33.0 27.0 35.0 2.0 36.0 88-89 27.27655213033161 33.0 26.0 35.0 2.0 35.0 90-91 26.78344225123349 32.5 25.0 35.0 2.0 35.0 92-93 26.015952736800948 32.0 23.5 34.0 2.0 35.0 94-95 25.25338839868575 32.0 20.5 34.0 2.0 35.0 96-97 24.26085016267329 31.0 10.0 34.0 2.0 35.0 98-99 22.868625111690264 31.0 2.0 34.0 2.0 35.0 100-101 18.42852803036166 24.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1560184736058119 1101 2 0.14358088681872516 1101 3 0.15991663287108082 1101 4 0.07088361925298159 1101 5 0.09635658650558554 1101 6 0.10572123224437036 1101 7 0.0929144063236862 1101 8 0.12236177294722239 1101 9 0.05432314438547081 1101 10-11 0.15447499068853432 1101 12-13 0.21164400705298192 1101 14-15 0.20467682450001945 1101 16-17 0.2542594747487641 1101 18-19 0.22521133763996204 1101 20-21 0.24100715694685704 1101 22-23 0.3196323673732451 1101 24-25 0.29441880189492053 1101 26-27 0.3251890165652114 1101 28-29 0.3369970049587252 1101 30-31 0.472394267027866 1101 32-33 0.2900405095638803 1101 34-35 0.35908345706515377 1101 36-37 0.4224698934041484 1101 38-39 0.35307870867891467 1101 40-41 0.3382941178494576 1101 42-43 0.4358220588398609 1101 44-45 0.4615971253532223 1101 46-47 0.5643166548616918 1101 48-49 0.3340998041155032 1101 50-51 0.22523588068184353 1101 52-53 0.10593153152249357 1101 54-55 0.07234880087498397 1101 56-57 0.2141818040849799 1101 58-59 0.44127127899219687 1101 60-61 0.40716802269319174 1101 62-63 0.4021401303188199 1101 64-65 0.46316235355072166 1101 66-67 0.22093225722372978 1101 68-69 0.27743735395616653 1101 70-71 0.38803606537911506 1101 72-73 0.3140697474979426 1101 74-75 0.31708617939521133 1101 76-77 0.20058658948398644 1101 78-79 0.23516543679859936 1101 80-81 -0.018623603009714884 1101 82-83 0.16790096153262368 1101 84-85 0.1902723334094567 1101 86-87 0.06793117290859385 1101 88-89 0.03852074264875327 1101 90-91 -0.08085038405420875 1101 92-93 -0.37437469612066465 1101 94-95 -0.39232685751479934 1101 96-97 -0.3311143692757348 1101 98-99 -0.02096229757168544 1101 100-101 -0.5528013751374843 1102 1 0.12228472311515048 1102 2 0.153743612811013 1102 3 0.0421215440904632 1102 4 0.04730653365876236 1102 5 0.13232110889123305 1102 6 0.05447380099451493 1102 7 0.020357605196899442 1102 8 0.09642461207997144 1102 9 0.08947547992774219 1102 10-11 0.0927046311789681 1102 12-13 0.07330945187968751 1102 14-15 0.06508892129283339 1102 16-17 0.07604536220864588 1102 18-19 0.13929761300997967 1102 20-21 0.11055693297493718 1102 22-23 0.16583638963189173 1102 24-25 0.11167272140357198 1102 26-27 0.09970629357461291 1102 28-29 0.11081624576318205 1102 30-31 0.12499909512364127 1102 32-33 0.045142076212847826 1102 34-35 0.1321785412277947 1102 36-37 0.14534648949272366 1102 38-39 0.2246775680384019 1102 40-41 0.2579926793493783 1102 42-43 0.2069428131865294 1102 44-45 0.19534472977724704 1102 46-47 0.07276178174063119 1102 48-49 -0.020003413460521813 1102 50-51 0.07818431518737867 1102 52-53 0.09996813476892896 1102 54-55 -0.004144537692063466 1102 56-57 0.003959621628673915 1102 58-59 -0.030604947658154913 1102 60-61 -0.15728047530953404 1102 62-63 0.041343298251582894 1102 64-65 0.09199311043727931 1102 66-67 0.0025975866009915194 1102 68-69 0.07398729161346296 1102 70-71 -0.062390376007197546 1102 72-73 -0.05725809429013751 1102 74-75 -0.15309929013768198 1102 76-77 -0.23499262038001234 1102 78-79 -0.1941351993278424 1102 80-81 -0.20590612511828255 1102 82-83 -0.1851114031029013 1102 84-85 -0.34066234529474215 1102 86-87 -0.5476481524048467 1102 88-89 -0.5886739512947656 1102 90-91 -0.78004267087681 1102 92-93 -0.5329661551707474 1102 94-95 -0.5147187102474859 1102 96-97 -0.7657271229758891 1102 98-99 -0.5879617953666028 1102 100-101 -1.4485169019332993 1103 1 0.1629807788381683 1103 2 0.18917664332231965 1103 3 0.13233929282436208 1103 4 0.11064456819947566 1103 5 0.0944948119937834 1103 6 0.02458165727442463 1103 7 0.10366178107136648 1103 8 0.14605850243850682 1103 9 0.1529847193100622 1103 10-11 0.21152092976907255 1103 12-13 0.22303819747732945 1103 14-15 0.26054792064208954 1103 16-17 0.1361917284966907 1103 18-19 0.2493622414525305 1103 20-21 0.2987268446382245 1103 22-23 0.38562656119964345 1103 24-25 0.35704042535549263 1103 26-27 0.24717266969170026 1103 28-29 0.3027419982680257 1103 30-31 0.3302187096578848 1103 32-33 0.3744921266739496 1103 34-35 0.34173354438455306 1103 36-37 0.38426340001547743 1103 38-39 0.36244399864738597 1103 40-41 0.38942189902928703 1103 42-43 0.4583387005581159 1103 44-45 0.5077545044101797 1103 46-47 0.4860750636072382 1103 48-49 0.4837245858169865 1103 50-51 0.40563338243957503 1103 52-53 0.468160075896634 1103 54-55 0.506107391063459 1103 56-57 0.5690504280671931 1103 58-59 0.6464149024074004 1103 60-61 0.4680012993256142 1103 62-63 0.404538578981807 1103 64-65 0.3775489347124079 1103 66-67 0.2418459641026871 1103 68-69 0.3050613305046639 1103 70-71 0.09762007912341986 1103 72-73 0.11225616876305367 1103 74-75 0.05690297874201633 1103 76-77 0.043060110157139064 1103 78-79 -0.055498615955929864 1103 80-81 0.2329531076568614 1103 82-83 0.18491378727937402 1103 84-85 0.07459679871983482 1103 86-87 -0.22702414677115712 1103 88-89 -0.39245893574366164 1103 90-91 -0.30790196487568977 1103 92-93 -0.26835674461128534 1103 94-95 -0.30415877899537236 1103 96-97 -0.49808963066695 1103 98-99 -0.5384050579989861 1103 100-101 -1.1339564011065022 1104 1 0.14202052717526925 1104 2 0.1295683114659738 1104 3 0.13126023037146695 1104 4 0.06774143716982195 1104 5 0.039558001922813446 1104 6 0.06534706305674831 1104 7 0.09551807845917892 1104 8 0.10990924794004542 1104 9 0.1714260105501566 1104 10-11 0.16263102884517622 1104 12-13 0.1933850420078258 1104 14-15 0.23462498843227308 1104 16-17 0.17897683293102062 1104 18-19 0.1458174166471835 1104 20-21 0.14023858649618148 1104 22-23 0.10973217526513679 1104 24-25 0.1390949782810722 1104 26-27 0.10743792353631676 1104 28-29 0.04080852923721778 1104 30-31 0.11488521365714632 1104 32-33 0.184691982392728 1104 34-35 0.0684354786165926 1104 36-37 0.003998532969589519 1104 38-39 0.16677193189971007 1104 40-41 0.20618501369153108 1104 42-43 0.16998211028949584 1104 44-45 0.09894614383534872 1104 46-47 0.1959603936829879 1104 48-49 0.11529377258327855 1104 50-51 0.1979660515918269 1104 52-53 0.15408428507208072 1104 54-55 0.20205379347770958 1104 56-57 0.2040014543157227 1104 58-59 0.15933689384855398 1104 60-61 -0.05020605816886814 1104 62-63 0.13340406919752468 1104 64-65 0.004180609341567276 1104 66-67 -0.20840880643692827 1104 68-69 -0.1736999757410942 1104 70-71 -0.42199896122896163 1104 72-73 -0.5567433445593792 1104 74-75 -0.40692485026009706 1104 76-77 -0.5429822050928266 1104 78-79 -0.4241130261157018 1104 80-81 -0.476957258363349 1104 82-83 -0.5896748880489326 1104 84-85 -0.5587119225580395 1104 86-87 -0.5846374450170799 1104 88-89 -0.7550408754756539 1104 90-91 -0.6513111399002582 1104 92-93 -0.8166718785350433 1104 94-95 -0.7132469063146765 1104 96-97 -1.0424538541168609 1104 98-99 -1.123542370219468 1104 100-101 -1.417655113517153 1105 1 0.13423189665618906 1105 2 0.1847170151831108 1105 3 0.16531259560400002 1105 4 0.09373989944621286 1105 5 0.10010486117777617 1105 6 0.12629145308633127 1105 7 0.11932054849069829 1105 8 0.1219951407871136 1105 9 0.16090958330887162 1105 10-11 0.18648413492594074 1105 12-13 0.20094266129729021 1105 14-15 0.10913248357524452 1105 16-17 0.12809919179500184 1105 18-19 0.16765440182216196 1105 20-21 0.20826043506970393 1105 22-23 0.1300188408031957 1105 24-25 0.13400429188801866 1105 26-27 0.08373370255555557 1105 28-29 0.07653289177722655 1105 30-31 0.09779756240398996 1105 32-33 0.18359209686546762 1105 34-35 0.06758285320041324 1105 36-37 0.06597403419090142 1105 38-39 0.11514306340631464 1105 40-41 0.01677281005097342 1105 42-43 0.01211389803930274 1105 44-45 -0.01563933082344704 1105 46-47 0.037649556863065925 1105 48-49 0.18485032171384574 1105 50-51 0.026833965567973905 1105 52-53 -0.13983278869834237 1105 54-55 0.045084308121353445 1105 56-57 0.07579110360187968 1105 58-59 0.09948168271890268 1105 60-61 0.013806221450963108 1105 62-63 0.05598545468044591 1105 64-65 0.09344616997584154 1105 66-67 -0.08296051365273627 1105 68-69 -0.23529772195825416 1105 70-71 -0.09117589114262259 1105 72-73 -0.023906874310199555 1105 74-75 -0.13737379299954355 1105 76-77 0.005139298248650448 1105 78-79 -0.0672543354581272 1105 80-81 -0.0486788134997056 1105 82-83 0.045864212050219066 1105 84-85 -0.08676139674927441 1105 86-87 -0.22209643233640008 1105 88-89 -0.3505104857846497 1105 90-91 -0.4442734344337822 1105 92-93 -0.23755620060651594 1105 94-95 -0.049016816106931316 1105 96-97 -0.2439874714151422 1105 98-99 -0.3203886260741058 1105 100-101 -0.6684608816937292 1106 1 0.1600167836380706 1106 2 0.0984337828088897 1106 3 0.09693908895752656 1106 4 0.1194932367057362 1106 5 0.10410291210603617 1106 6 0.10600673436963604 1106 7 0.13295703960069005 1106 8 0.06523894301943045 1106 9 0.07438446972987123 1106 10-11 0.02204873473524316 1106 12-13 -0.03340867641093581 1106 14-15 0.05395026640098166 1106 16-17 0.13917139158186842 1106 18-19 0.2620827208529519 1106 20-21 0.30243460355923446 1106 22-23 0.2936427120242371 1106 24-25 0.23850135425831098 1106 26-27 0.26735659222968167 1106 28-29 0.1823015832349668 1106 30-31 0.2025365059790687 1106 32-33 0.2524300855356074 1106 34-35 0.2705483518262497 1106 36-37 0.14583689806435984 1106 38-39 0.3312358756784519 1106 40-41 0.32233800579947314 1106 42-43 0.3091080858793447 1106 44-45 0.241305990002175 1106 46-47 0.2097050913854801 1106 48-49 0.1412223139736497 1106 50-51 0.18913362483097984 1106 52-53 0.033247235419665344 1106 54-55 -0.001845903892778722 1106 56-57 -0.04781632670828628 1106 58-59 0.16291502867212415 1106 60-61 0.1312500629451563 1106 62-63 0.08696114737212923 1106 64-65 0.019486609708856406 1106 66-67 -0.04622699662991181 1106 68-69 -0.003054080980078311 1106 70-71 -0.5863356379035203 1106 72-73 -0.5591961151537674 1106 74-75 -0.41095896322782366 1106 76-77 -0.3584598590232062 1106 78-79 -0.2351829910192933 1106 80-81 -0.25447940523227786 1106 82-83 -0.3224630216701243 1106 84-85 -0.37598208355167273 1106 86-87 -0.42535376589408713 1106 88-89 -0.670191553146811 1106 90-91 -0.6085405694385457 1106 92-93 -0.5619259072642109 1106 94-95 -0.7649010496590058 1106 96-97 -0.9844498150687393 1106 98-99 -0.8805819908169354 1106 100-101 -1.5467779844476688 1107 1 -0.04381503744986048 1107 2 -0.02861772292314413 1107 3 -0.015512667171165617 1107 4 -0.038077610451466626 1107 5 -0.028397200992152705 1107 6 0.007541544901556563 1107 7 0.02086065705302076 1107 8 -0.062173114282920494 1107 9 -0.03040740507834272 1107 10-11 -0.0652247759251523 1107 12-13 -0.0345744053164907 1107 14-15 0.008649673221420073 1107 16-17 -0.02556115982057605 1107 18-19 0.017857405281759497 1107 20-21 0.00880173172102161 1107 22-23 0.07875656510778839 1107 24-25 0.035031038580832785 1107 26-27 -0.09904883125081199 1107 28-29 0.0052989686228954724 1107 30-31 -0.09565217886070343 1107 32-33 0.012561911692522187 1107 34-35 0.09766714416653599 1107 36-37 -0.02097039189050065 1107 38-39 -0.0771664475490823 1107 40-41 -0.16436014849261227 1107 42-43 -0.18529927689440484 1107 44-45 -0.200573669166225 1107 46-47 -0.17933563976841072 1107 48-49 -0.25097290276335826 1107 50-51 -0.3359469201551235 1107 52-53 -0.30629723233481343 1107 54-55 -0.26492359716940683 1107 56-57 -0.25132002679041676 1107 58-59 -0.2649670265426849 1107 60-61 -0.46254607907128786 1107 62-63 -0.36731543574359193 1107 64-65 -0.4650594087171527 1107 66-67 -0.4561268488664467 1107 68-69 -0.44635509820970043 1107 70-71 -0.6570669716608997 1107 72-73 -0.8062773643464141 1107 74-75 -0.7265447382556331 1107 76-77 -0.6976388697438267 1107 78-79 -0.6972922367824701 1107 80-81 -0.5489186807716209 1107 82-83 -0.710738909300126 1107 84-85 -0.7360349317455608 1107 86-87 -0.8611349800399175 1107 88-89 -0.8836615199913282 1107 90-91 -0.8144350016829236 1107 92-93 -0.9119541681847245 1107 94-95 -1.169700582440857 1107 96-97 -1.2553972344951383 1107 98-99 -1.4691976908075084 1107 100-101 -2.270120877984393 1108 1 -9.488721768562414E-4 1108 2 -0.02639377278028121 1108 3 -0.005013718960910296 1108 4 0.03670594308408681 1108 5 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#Quality Count 2 3.0 3 3.0 4 16.0 5 21.0 6 72.0 7 216.0 8 428.0 9 807.0 10 1453.0 11 2516.0 12 4102.0 13 6246.0 14 7904.0 15 11205.0 16 15341.0 17 22326.0 18 22570.0 19 15151.0 20 13203.0 21 13833.0 22 15048.0 23 16162.0 24 18234.0 25 21064.0 26 24220.0 27 29165.0 28 35154.0 29 42478.0 30 52590.0 31 64181.0 32 80930.0 33 106209.0 34 145699.0 35 212078.0 36 311743.0 37 413958.0 38 250089.0 39 13460.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.16703288750219 19.37742122901434 14.93497486043066 22.520571023052817 2 15.509575493652358 20.580048432868004 40.070658910169996 23.83971716330964 3 18.88771897200325 25.0119741970503 30.364402584315688 25.735904246630763 4 12.827419570446027 17.221558306589653 36.962869080415985 32.98815304254833 5 13.445653075224072 37.757977340057444 33.89674121983763 14.899628364880858 6 31.35618646150413 36.49901976895595 17.301618172744803 14.843175596795113 7 28.011817809936083 33.75955711857712 20.39165215153894 17.836972919947854 8 26.413830395632292 34.728008450769345 19.736637120466682 19.121524033131678 9 26.882767125807273 16.562531251931652 20.289193754186815 36.26550786807426 10-11 25.096940616459907 26.810663769336614 26.79742175148426 21.29497386271922 12-13 25.8901947599818 24.327019068431717 27.808119115947633 21.974667055638854 14-15 22.836048240143366 25.49937734876209 26.445892662766262 25.218681748328294 16-17 21.650847400556987 28.86815670056671 27.119632147723877 22.361363751152428 18-19 21.54162214969963 26.920896657986066 30.222229704534648 21.31525148777965 20-21 22.51914438975656 26.117656459340722 30.173658887630296 21.18954026327242 22-23 22.193923446563055 25.93621317487806 30.16139682935336 21.70846654920553 24-25 21.903856340341356 26.28278321491355 30.208577284107964 21.604783160637126 26-27 21.61496849102481 26.516043503119484 30.17329622206445 21.695691783791254 28-29 21.45815319257791 26.603513432626542 30.426530590193256 21.511802784602292 30-31 21.642233970911942 26.324667557869557 30.253515393352163 21.77958307786634 32-33 21.510414206298076 26.40717169595322 30.120866706401095 21.96154739134761 34-35 22.036156534408665 26.553197841192393 29.81719190665773 21.593453717741205 36-37 22.127455668374743 26.231487081632103 29.8425693660435 21.798487883949655 38-39 21.736560846042337 26.43364670189079 29.923669339508734 21.906123112558138 40-41 21.934477143890966 26.37109013790053 29.900117597394765 21.794315120813735 42-43 21.986508313825688 26.266547995846857 29.639092620531127 22.107851069796332 44-45 21.823227811762283 26.59862227699946 29.539657591987446 22.038492319250807 46-47 22.057985093022246 26.924852258196314 28.938246576980898 22.078916071800545 48-49 22.103604489851197 26.786207409495766 28.895772101179702 22.21441599947333 50-51 22.109926957262914 27.100443563504562 28.62597836760991 22.16365111162261 52-53 22.243434055337 27.156920517611166 28.368223127152476 22.231422299899357 54-55 21.912699100756257 27.13941984517981 28.454876358697362 22.493004695366576 56-57 21.981390691928095 27.365066935003128 28.437474554445192 22.216067818623582 58-59 22.031580502987847 26.952480959006568 28.51185186227752 22.50408667572806 60-61 22.012796235207084 27.050453210466962 28.670462015354275 22.266288538971676 62-63 21.78295608167968 27.187050520632667 28.547172101699235 22.482821295988423 64-65 21.66971610101335 27.014737815666724 28.486559031687307 22.82898705163262 66-67 21.700127688758087 27.22931981171123 28.31875798368871 22.751794515841976 68-69 21.670991172779413 27.167122995690075 28.385374447434693 22.77651138409582 70-71 21.69723408008999 27.168909148208854 28.200279763583186 22.933577008117968 72-73 21.90729317541957 27.08035770824685 28.033252955857385 22.979096160476196 74-75 21.835413293820032 27.449698196719748 27.72589255511698 22.98899595434324 76-77 21.756999762496278 27.185128497053825 28.024031858171323 23.033839882278578 78-79 21.80274906074093 27.257991870646155 28.041570696524566 22.89768837208835 80-81 21.7032487901035 27.57709587647571 27.844934216511284 22.874721116909498 82-83 21.74892158792329 27.481541752852912 27.79592203007162 22.973614629152177 84-85 21.78746757540564 27.238418566986866 27.750199995124213 23.22391386248328 86-87 21.839335280318334 27.113613587572477 27.877024747521588 23.1700263845876 88-89 21.752705576025054 27.121508386908683 27.752474351692207 23.373311685374055 90-91 21.664438497633466 27.197938572911145 27.85837139317494 23.279251536280448 92-93 21.734316823809667 26.90254285638167 27.830873685580965 23.5322666342277 94-95 21.541485387782675 27.00360078167723 27.786031942612667 23.66888188792743 96-97 21.623113871745918 26.873399299392304 27.82506772231126 23.678419106550525 98-99 21.526530864572933 26.899097849832295 27.536400511550983 24.03797077404379 100-101 21.920980792906107 26.34516377332659 27.515391483601054 24.218463950166246 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 975.0 1 669.0 2 525.5 3 716.0 4 592.5 5 555.0 6 1133.0 7 2488.0 8 3941.0 9 4474.0 10 3819.0 11 3141.0 12 3136.5 13 3314.0 14 3548.5 15 3699.5 16 3789.0 17 3901.0 18 4114.0 19 4279.5 20 4449.5 21 5139.5 22 6077.5 23 7080.0 24 8347.5 25 10136.0 26 12466.0 27 15178.5 28 17617.5 29 20197.5 30 23525.5 31 27293.5 32 30670.0 33 33234.5 34 35740.5 35 38092.0 36 41690.5 37 45700.5 38 48824.0 39 50995.5 40 53015.5 41 58380.5 42 63805.0 43 67197.5 44 70963.5 45 74554.5 46 75397.0 47 76452.5 48 80896.0 49 80905.0 50 77289.5 51 74346.5 52 71563.5 53 70344.5 54 68483.0 55 62656.0 56 54576.0 57 48326.0 58 43813.5 59 38002.5 60 31308.5 61 25648.0 62 20669.0 63 15685.0 64 11787.0 65 9000.5 66 6780.5 67 5670.0 68 4426.5 69 3087.0 70 2316.5 71 1828.0 72 1533.0 73 1289.0 74 1030.5 75 902.5 76 604.0 77 273.5 78 131.5 79 76.5 80 32.0 81 14.5 82 10.0 83 4.5 84 3.0 85 3.0 86 3.5 87 2.5 88 2.0 89 3.5 90 3.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00934730671930641 2 0.016081387904183068 3 0.009196543707704693 4 0.0 5 0.0011558497556131582 6 0.0020604278252234556 7 0.0 8 0.0 9 5.0254337200572095E-5 10-11 0.0 12-13 9.297052382105837E-4 14-15 0.0 16-17 0.0028896243890328957 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.003216277580836614 26-27 0.0183679602468091 28-29 0.005754121609465505 30-31 0.0029147515576331815 32-33 0.0 34-35 0.008467855818296397 36-37 8.794509010100118E-4 38-39 0.0024373353542277466 40-41 0.0019096648136217396 42-43 0.003015260232034326 44-45 2.5127168600286047E-5 46-47 2.261445174025744E-4 48-49 6.281792150071512E-4 50-51 0.0042213643248480565 52-53 0.008065821120691822 54-55 0.007563277748686101 56-57 0.016885457299392226 58-59 0.02442360787947804 60-61 0.003718820952842335 62-63 0.021282711804442283 64-65 0.02201139969385058 66-67 0.01379481556155704 68-69 0.03183612261656242 70-71 0.017136728985395082 72-73 0.005025433720057209 74-75 0.016935711636592795 76-77 0.021885763850849147 78-79 0.027338359437111222 80-81 0.012312312614140163 82-83 0.03650977597621563 84-85 0.02284059625766002 86-87 0.032589937674571 88-89 0.023519029809867743 90-91 0.028971625396129814 92-93 0.025177422937486617 94-95 0.01557884453217735 96-97 0.016056260735582785 98-99 0.012186676771138733 100-101 0.01118159002712729 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1989878.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.32582611974654 #Duplication Level Percentage of deduplicated Percentage of total 1 70.5188831911136 33.37364404061359 2 14.932761741947198 14.134105713739931 3 5.806448607877863 8.243849315690161 4 2.8820910605183983 5.455893615654785 5 1.599029427993704 3.7837694334793905 6 0.9832227799370319 2.7919098192164276 7 0.653670761311518 2.165485615257387 8 0.4463946998757067 1.6900798357675308 9 0.32803769972356267 1.3972189624055058 >10 1.6620265523717375 14.238088187144484 >50 0.11906224716275603 3.8984308393879923 >100 0.06379991444742641 5.698122664155531 >500 0.0032955997047776175 1.0727006291894845 >1k 9.567870110644694E-4 0.6566712917865557 >5k 2.126193357921043E-4 0.801267774516755 >10k+ 1.0630966789605215E-4 0.5987622619944682 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11901 0.5980768670240085 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9536 0.47922535954465545 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6390 0.32112521471165567 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2255 0.11332353038729008 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 5.0254337200572095E-5 0.0 16-17 0.0 0.0 0.0 5.0254337200572095E-5 0.0 18-19 0.0 0.0 0.0 5.0254337200572095E-5 0.0 20-21 0.0 0.0 0.0 1.5076301160171627E-4 0.0 22-23 0.0 0.0 0.0 3.7690752900429073E-4 0.0 24-25 0.0 0.0 0.0 7.035607208080094E-4 0.0 26-27 0.0 0.0 0.0 0.0020604278252234556 0.0 28-29 0.0 0.0 0.0 0.006935098533678949 0.0 30-31 0.0 0.0 0.0 0.020880677106837706 0.0 32-33 0.0 0.0 0.0 0.050756880572577816 0.0 34-35 0.0 0.0 0.0 0.097066252302905 0.0 36-37 0.0 0.0 0.0 0.1732769546675726 0.0 38-39 0.0 0.0 0.0 0.2885101498684844 0.0 40-41 0.0 0.0 0.0 0.4120353107074906 0.0 42-43 0.0 0.0 0.0 0.5308616910182433 0.0 44-45 0.0 0.0 0.0 0.6757198179988924 0.0 46-47 0.0 0.0 0.0 0.8412576047375768 0.0 48-49 0.0 0.0 0.0 1.0119967153765206 0.0 50-51 0.0 0.0 0.0 1.197812127175636 0.0 52-53 0.0 0.0 0.0 1.3708880644944061 0.0 54-55 0.0 0.0 0.0 1.5562763144273166 0.0 56-57 0.0 0.0 0.0 1.7667917329605132 0.0 58-59 0.0 0.0 0.0 1.9872323830908227 0.0 60-61 0.0 0.0 0.0 2.2200858545096738 0.0 62-63 0.0 0.0 0.0 2.4517583490043107 0.0 64-65 0.0 0.0 0.0 2.689411109625816 0.0 66-67 0.0 0.0 0.0 2.9494773046387768 0.0 68-69 0.0 0.0 0.0 3.2350978301182285 0.0 70-71 0.0 0.0 0.0 3.5308446045435953 0.0 72-73 0.0 0.0 0.0 3.8372955527926838 0.0 74-75 0.0 0.0 0.0 4.1427162871291605 0.0 76-77 0.0 0.0 0.0 4.465525022137035 0.0 78-79 0.0 0.0 0.0 4.81293325520459 0.0 80-81 0.0 0.0 0.0 5.182453396640397 0.0 82-83 0.0 0.0 0.0 5.556320538244053 0.0 84-85 0.0 0.0 0.0 5.935087477724765 0.0 86-87 0.0 0.0 0.0 6.318904978094134 0.0 88-89 0.0 0.0 0.0 6.72702045049998 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6975 0.0 42.356598 1 GTATCAA 10870 0.0 36.96713 1 TATCAAC 13795 0.0 29.162561 2 ATCAACG 13875 0.0 29.059229 3 TCAACGC 14155 0.0 28.482979 4 CAACGCA 14230 0.0 28.299484 5 AACGCAG 14530 0.0 27.682505 6 GTACATG 9520 0.0 26.044046 1 TACATGG 9760 0.0 24.916332 2 ACGCAGA 16370 0.0 24.252476 7 CGCAGAG 16445 0.0 24.026358 8 ACATGGG 10015 0.0 23.520151 3 GAGTACT 9485 0.0 23.383566 12-13 GCAGAGT 17385 0.0 22.59068 9 GTACTTT 10105 0.0 21.61931 14-15 AGTACTT 9755 0.0 20.886286 12-13 AGAGTAC 15330 0.0 20.214045 10-11 CAGAGTA 16890 0.0 19.781046 10-11 TATCGTG 170 7.972526E-5 19.554049 5 CATGGGG 5100 0.0 19.554047 4 >>END_MODULE