FastQCFastQC Report
Thu 26 May 2016
SRR938103_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938103_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2515157
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98190.390393124564391No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80700.3208547219915099No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51330.20408268748233213No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA28530.11343228275610627No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA26140.1039298938396291No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43850.059.8143541
GTATCAA83150.046.6846961
TATCAAC106550.035.9497832
ATCAACG106400.035.9111333
TCAACGC109000.035.0981374
CAACGCA109800.034.7558485
AACGCAG113650.033.5737886
CGCAGAG129850.028.8688058
ACGCAGA130250.028.8160487
TGGTATC19150.027.2984892
GTGGTAT20550.027.151041
GCAGAGT141550.025.9429999
TAAGGTG13750.023.8485015
CAGAGTA134900.022.92553710-11
GAGTACT92900.022.8317412-13
TACATGG82100.022.401782
GTACATG84550.022.3354111
GTGTAGC16450.022.322211
GTAAGGT13450.021.9070594
GTACTTT97850.021.48361214-15
Produced by FastQC (version 0.11.5)