FastQCFastQC Report
Thu 26 May 2016
SRR938103_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938103_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2515157
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96740.3846280768953986No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82410.3276535023459768No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50870.20225377580803108No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA27770.11041060259856543No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA26200.10416844753627706No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46200.055.5929071
GTATCAA77850.041.239441
ATCAACG101400.031.2357543
TATCAAC102150.031.1963672
TCAACGC102100.031.0669424
CAACGCA103800.030.6491585
TGGTATC19500.030.0011652
AACGCAG107900.029.4470356
GTGGTAT22150.028.7738931
ACGCAGA123400.025.4788447
CGCAGAG124000.025.2406568
GCAGAGT138600.022.444769
TAAGGTG14650.022.3975875
GAGTACT88700.021.68544412-13
GTACATG85750.021.632071
TACATGG82650.021.4075852
CAGAGTA130100.020.93596810-11
GTAAGGT14550.020.917774
CTATCGG1404.8108707E-420.3840661
GTACTTT94450.020.31497614-15
Produced by FastQC (version 0.11.5)