##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938103_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2515157 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.453039313251615 33.0 31.0 34.0 31.0 34.0 2 32.6822902109093 34.0 31.0 34.0 31.0 34.0 3 32.750748760415355 34.0 31.0 34.0 31.0 34.0 4 36.21814622307872 37.0 35.0 37.0 35.0 37.0 5 36.14001591153156 37.0 35.0 37.0 35.0 37.0 6 36.19325195206502 37.0 37.0 37.0 35.0 37.0 7 36.16319617423485 37.0 37.0 37.0 35.0 37.0 8 36.1516279103054 37.0 36.0 37.0 35.0 37.0 9 37.8401022282108 39.0 38.0 39.0 35.0 39.0 10-11 37.812663185638115 39.0 38.0 39.0 35.0 39.0 12-13 37.73083111710322 39.0 38.0 39.0 35.0 39.0 14-15 38.970518142605016 40.0 38.0 41.0 35.5 41.0 16-17 38.87493444743211 40.0 38.0 41.0 35.0 41.0 18-19 39.12030084006685 40.0 38.5 41.0 36.0 41.0 20-21 39.08793427209514 40.0 39.0 41.0 36.0 41.0 22-23 38.98679982999073 40.0 38.5 41.0 35.0 41.0 24-25 38.880296736943265 40.0 38.0 41.0 35.0 41.0 26-27 38.71674770203212 40.0 38.0 41.0 35.0 41.0 28-29 38.59262046067104 40.0 38.0 41.0 34.0 41.0 30-31 38.35203766603834 40.0 38.0 41.0 34.0 41.0 32-33 38.128281653988196 40.0 38.0 41.0 34.0 41.0 34-35 38.042303721000316 40.0 38.0 41.0 33.0 41.0 36-37 37.992202276040814 40.0 38.0 41.0 33.0 41.0 38-39 37.8012458069218 40.0 37.0 41.0 33.0 41.0 40-41 37.57832771473113 40.0 37.0 41.0 33.0 41.0 42-43 37.324490876712666 40.0 37.0 41.0 32.0 41.0 44-45 37.05591718528903 40.0 36.0 41.0 31.0 41.0 46-47 36.68873931130343 39.0 36.0 41.0 30.5 41.0 48-49 36.26944600277438 39.0 35.0 41.0 30.0 41.0 50-51 35.87371742598971 39.0 35.0 40.0 28.5 41.0 52-53 35.3272851356794 38.0 34.5 40.0 27.5 41.0 54-55 34.940802900176806 38.0 34.0 40.0 26.5 41.0 56-57 34.54555282234867 38.0 34.0 40.0 26.0 41.0 58-59 34.02522287873083 37.0 33.0 40.0 24.5 41.0 60-61 33.53486919504429 37.0 33.0 39.5 22.5 41.0 62-63 32.95860318063644 36.0 32.0 39.0 21.5 40.0 64-65 32.37413390098511 35.5 31.0 39.0 19.0 40.0 66-67 31.79887120366641 35.0 31.0 38.0 16.5 40.0 68-69 31.161277009745316 35.0 30.0 38.0 10.5 40.0 70-71 30.534243587974828 34.5 29.0 37.0 7.5 39.0 72-73 29.860757400035066 34.0 29.0 36.5 4.5 39.0 74-75 29.1903781751994 34.0 28.0 36.0 2.0 38.5 76-77 27.909354763937202 31.5 26.5 34.5 2.0 36.5 78-79 28.55244861453977 33.5 28.0 35.0 2.0 37.0 80-81 28.27831264608929 33.5 27.0 35.0 2.0 37.0 82-83 27.71776612752206 33.0 26.0 35.0 2.0 36.0 84-85 27.056878357891776 33.0 25.5 35.0 2.0 36.0 86-87 26.3323204078314 32.0 24.5 35.0 2.0 35.0 88-89 25.51053492883347 32.0 21.0 34.0 2.0 35.0 90-91 24.841877067713863 31.0 18.5 34.0 2.0 35.0 92-93 23.886803289019333 31.0 9.0 34.0 2.0 35.0 94-95 22.96154852361105 30.5 2.0 34.0 2.0 35.0 96-97 21.89538903535644 30.0 2.0 34.0 2.0 35.0 98-99 20.463021195098356 29.0 2.0 34.0 2.0 35.0 100-101 16.62008137066593 20.5 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2054192819694336 1101 2 0.1276734195606437 1101 3 0.17233982509428358 1101 4 0.09564123791051315 1101 5 0.023832311044493792 1101 6 0.068627934846198 1101 7 0.10203238549205196 1101 8 0.10426095736669083 1101 9 0.010792378475578346 1101 10-11 0.12349406537381924 1101 12-13 0.2508428468343027 1101 14-15 0.40544642320745083 1101 16-17 0.4300299311266045 1101 18-19 0.3395268422115336 1101 20-21 0.2946020546965826 1101 22-23 0.37731593314531864 1101 24-25 0.3566836539820315 1101 26-27 0.3202944592964343 1101 28-29 0.39026244775641317 1101 30-31 0.4606294811990779 1101 32-33 0.5164079648722719 1101 34-35 0.42246531050755465 1101 36-37 0.4149840373264837 1101 38-39 0.5494483039031479 1101 40-41 0.6796680900905443 1101 42-43 0.7520607172242606 1101 44-45 0.6745814504054692 1101 46-47 0.7561803725045237 1101 48-49 0.8313820828080409 1101 50-51 0.8829366671056036 1101 52-53 0.871501548207597 1101 54-55 0.7566527237558134 1101 56-57 0.9593714826978257 1101 58-59 1.1879386102376586 1101 60-61 1.1275130349900024 1101 62-63 1.1719175967395827 1101 64-65 1.2837980744571738 1101 66-67 1.569490626238153 1101 68-69 1.7194988338683181 1101 70-71 1.8868843711548386 1101 72-73 2.0138863086512693 1101 74-75 2.0833438475441817 1101 76-77 2.0725735003617167 1101 78-79 2.1657363106002983 1101 80-81 2.285711419787603 1101 82-83 2.4226762146367555 1101 84-85 2.624231515849573 1101 86-87 2.8587018796730597 1101 88-89 3.1526086257285506 1101 90-91 3.439955590935874 1101 92-93 3.5447250386679414 1101 94-95 3.7466647359296346 1101 96-97 3.7100673336524572 1101 98-99 3.8989733328123393 1101 100-101 4.129688576833441 1102 1 0.22349123870709775 1102 2 0.16923432159500607 1102 3 0.20457504563653828 1102 4 0.12133744509883115 1102 5 0.03977977821147505 1102 6 0.11320542813244572 1102 7 0.12301976731157538 1102 8 0.17427714395390126 1102 9 0.2770433441526592 1102 10-11 0.2748064665068739 1102 12-13 0.3693222273294623 1102 14-15 0.44820292543310103 1102 16-17 0.33176539078889533 1102 18-19 0.380053090234199 1102 20-21 0.31800822767233683 1102 22-23 0.37943122579145694 1102 24-25 0.40134851805148486 1102 26-27 0.4274478272102016 1102 28-29 0.37229625565105096 1102 30-31 0.387946186492826 1102 32-33 0.4627485085944443 1102 34-35 0.4595013256640854 1102 36-37 0.5136394469574981 1102 38-39 0.6199289351800417 1102 40-41 0.6889064007994463 1102 42-43 0.7762164395507156 1102 44-45 0.7529622398694755 1102 46-47 0.7352251799209242 1102 48-49 0.7270797615042923 1102 50-51 0.671522364048549 1102 52-53 0.768932847181226 1102 54-55 0.9358547985819925 1102 56-57 1.046084685802711 1102 58-59 1.3037324887646733 1102 60-61 1.1672069613410372 1102 62-63 1.3526601564276248 1102 64-65 1.2917672095783885 1102 66-67 1.4071557110337984 1102 68-69 1.5663056616651083 1102 70-71 1.725299005301185 1102 72-73 2.0568543401415376 1102 74-75 2.1565559654119646 1102 76-77 2.158123292511224 1102 78-79 2.2919503046406895 1102 80-81 2.3019301962287635 1102 82-83 2.528408657707793 1102 84-85 2.790461328600191 1102 86-87 2.7149079643583427 1102 88-89 3.061338346509924 1102 90-91 3.1266991438917664 1102 92-93 3.2416863747971405 1102 94-95 3.34513160860741 1102 96-97 3.448814896467457 1102 98-99 3.977206971483568 1102 100-101 4.030683684069579 1103 1 0.2601467398861317 1103 2 0.13002446237955922 1103 3 0.1314959321470539 1103 4 0.12624860280769212 1103 5 0.04259319465692357 1103 6 0.08059123813437452 1103 7 0.14858230316905008 1103 8 0.16551949541233313 1103 9 0.23159691701041396 1103 10-11 0.2334892829730535 1103 12-13 0.2833193637862621 1103 14-15 0.4058005399053357 1103 16-17 0.3735501431585462 1103 18-19 0.2625137617978339 1103 20-21 0.30378170868441146 1103 22-23 0.29871662896637474 1103 24-25 0.3062840862234424 1103 26-27 0.4036104518636279 1103 28-29 0.39901497129421415 1103 30-31 0.3807871178899518 1103 32-33 0.4697558279422793 1103 34-35 0.5500039074022851 1103 36-37 0.5725495397888665 1103 38-39 0.5974785754047787 1103 40-41 0.7355747201043101 1103 42-43 0.7407143801416751 1103 44-45 0.8108762215256178 1103 46-47 0.840478400038009 1103 48-49 1.0166179719877704 1103 50-51 1.124029902016403 1103 52-53 1.229113779471355 1103 54-55 1.2825543523275513 1103 56-57 1.3702125646156063 1103 58-59 1.5361229407563073 1103 60-61 1.5836810542364361 1103 62-63 1.671432055287859 1103 64-65 1.725912771760413 1103 66-67 1.7625499389597046 1103 68-69 1.818552940709349 1103 70-71 1.8284989242559213 1103 72-73 2.0451507973177137 1103 74-75 1.9939093025476318 1103 76-77 2.0890271494065153 1103 78-79 2.1864466613948714 1103 80-81 2.314895016518051 1103 82-83 2.4377991880487997 1103 84-85 2.605291748544399 1103 86-87 3.006397748189894 1103 88-89 3.328482326540101 1103 90-91 3.4500552182006707 1103 92-93 3.6799100007555516 1103 94-95 3.629890191444183 1103 96-97 3.757060809873707 1103 98-99 4.1909662796838845 1103 100-101 4.123005993168807 1104 1 0.19735809428989626 1104 2 0.19657126743803843 1104 3 0.15087882807679165 1104 4 0.09235294114046155 1104 5 -0.004168508071074939 1104 6 0.10133592120187984 1104 7 0.1262410968157326 1104 8 0.15110375924068364 1104 9 0.16310793323935258 1104 10-11 0.21222651361431133 1104 12-13 0.3069238395279825 1104 14-15 0.3945929305650182 1104 16-17 0.3309870030039974 1104 18-19 0.35268805983655227 1104 20-21 0.3603860588234369 1104 22-23 0.3947349244962126 1104 24-25 0.40166801872261004 1104 26-27 0.4167622241612321 1104 28-29 0.44692396430789216 1104 30-31 0.42237619232115264 1104 32-33 0.33702017556925057 1104 34-35 0.359908106083914 1104 36-37 0.37538446987698393 1104 38-39 0.35682173881864543 1104 40-41 0.42826670718846316 1104 42-43 0.4868019952601159 1104 44-45 0.5459485006151823 1104 46-47 0.46451214727817103 1104 48-49 0.5626520521841982 1104 50-51 0.7199545411963868 1104 52-53 0.8111418601055007 1104 54-55 0.8838701843580665 1104 56-57 0.8544554790266048 1104 58-59 1.003897120216557 1104 60-61 1.112698053459198 1104 62-63 1.2179068362322027 1104 64-65 1.2685508171523807 1104 66-67 1.28529672492974 1104 68-69 1.4698890021145736 1104 70-71 1.4687635947195012 1104 72-73 1.566353344849798 1104 74-75 1.6655051968278691 1104 76-77 1.7873536969536836 1104 78-79 1.7931861286767585 1104 80-81 1.9196259321021287 1104 82-83 2.259133429901638 1104 84-85 2.488275977172247 1104 86-87 2.5489166396876293 1104 88-89 2.578843211044955 1104 90-91 2.8914079889305206 1104 92-93 3.152487342149417 1104 94-95 3.6512883855037117 1104 96-97 3.992912103432591 1104 98-99 4.141890550786478 1104 100-101 3.6763674921942453 1105 1 0.20865977076377362 1105 2 0.16268389136921968 1105 3 0.12990014106255643 1105 4 0.1475715138055662 1105 5 0.06294080557771053 1105 6 0.09271309456189414 1105 7 0.10422569342918564 1105 8 0.12188987604938717 1105 9 0.15861803523113593 1105 10-11 0.1824454003232745 1105 12-13 0.2353618857366584 1105 14-15 0.35712768444196996 1105 16-17 0.3841930934478839 1105 18-19 0.3196840017952951 1105 20-21 0.291790332867393 1105 22-23 0.30627666798593367 1105 24-25 0.3844710515314631 1105 26-27 0.3384428456484514 1105 28-29 0.34815670722638714 1105 30-31 0.4660014365413474 1105 32-33 0.5007167430513277 1105 34-35 0.4883717403629291 1105 36-37 0.5772352288039073 1105 38-39 0.5664175811009926 1105 40-41 0.5692276144890371 1105 42-43 0.6284274119256921 1105 44-45 0.7400797270920947 1105 46-47 0.803390089023587 1105 48-49 0.8569885406981825 1105 50-51 0.9003264657500409 1105 52-53 1.0507549989571174 1105 54-55 1.2643952656842785 1105 56-57 1.05971119806253 1105 58-59 1.3154019589076498 1105 60-61 1.313968946202266 1105 62-63 1.315694703881853 1105 64-65 1.389530358960542 1105 66-67 1.4972928657209899 1105 68-69 1.6254050988133208 1105 70-71 1.8549247412137184 1105 72-73 1.979300481048572 1105 74-75 1.9240156923965053 1105 76-77 1.7516084448693796 1105 78-79 2.0947176592373147 1105 80-81 2.274588215249274 1105 82-83 2.3347666185022575 1105 84-85 2.5709572204291327 1105 86-87 2.700594061272362 1105 88-89 2.9458728616163476 1105 90-91 2.96180621512163 1105 92-93 3.0972696056957645 1105 94-95 3.2187083052497094 1105 96-97 3.5753190693341175 1105 98-99 3.6323874722275704 1105 100-101 3.2800043801943133 1106 1 0.09397460184482043 1106 2 0.06374433825924086 1106 3 0.07728621915821066 1106 4 0.07910274076373724 1106 5 -0.031404122947463975 1106 6 -0.017861576600012086 1106 7 0.01782262267200707 1106 8 0.05392545219834233 1106 9 0.1144112582851804 1106 10-11 0.10828679753991821 1106 12-13 0.0814296904556926 1106 14-15 0.04403019571378053 1106 16-17 0.0015014658172916029 1106 18-19 -0.08195952322874689 1106 20-21 -0.11372179934053861 1106 22-23 -0.07845128246547262 1106 24-25 -0.008246547144167948 1106 26-27 -0.1309026446090371 1106 28-29 0.08363065546474502 1106 30-31 0.15115419488894588 1106 32-33 0.23010609336729715 1106 34-35 0.10098714178375445 1106 36-37 0.15815466878618167 1106 38-39 0.14518005544709922 1106 40-41 0.19866058980012014 1106 42-43 0.12737273791537262 1106 44-45 0.3097290886012303 1106 46-47 0.414778580666912 1106 48-49 0.5424442888098113 1106 50-51 0.599311406830374 1106 52-53 0.6083630667466267 1106 54-55 0.6579049933322523 1106 56-57 0.6908774200100467 1106 58-59 0.9962091561330766 1106 60-61 0.7984260725457091 1106 62-63 0.9008415683612583 1106 64-65 0.954105203654521 1106 66-67 1.0362181491019804 1106 68-69 0.9786952254260619 1106 70-71 1.1219900822612736 1106 72-73 1.4420677403168973 1106 74-75 1.4466680229633475 1106 76-77 1.4135518934632678 1106 78-79 1.679431903420447 1106 80-81 1.5529898327617673 1106 82-83 2.0865176677317834 1106 84-85 2.120166180353248 1106 86-87 2.2456128438722835 1106 88-89 2.408478860502562 1106 90-91 2.51758675166759 1106 92-93 2.360461672860282 1106 94-95 2.499579730082843 1106 96-97 3.081222616613136 1106 98-99 3.3041550139176117 1106 100-101 3.160270879827717 1107 1 0.18606718642276832 1107 2 0.17043255600475504 1107 3 0.18542115599525033 1107 4 0.1180921945490283 1107 5 -0.02686628368979882 1107 6 0.03473544068167911 1107 7 0.11477423719962587 1107 8 0.07114045670317637 1107 9 0.15225068607579573 1107 10-11 0.1872899257467111 1107 12-13 0.22281186178642542 1107 14-15 0.2866346439326648 1107 16-17 0.30254143976818426 1107 18-19 0.24045800493668423 1107 20-21 0.1830251411820285 1107 22-23 0.27932274014852254 1107 24-25 0.355480135908671 1107 26-27 0.33138483875301006 1107 28-29 0.3449772100140862 1107 30-31 0.3701225467179654 1107 32-33 0.38987209369938114 1107 34-35 0.4136294489944774 1107 36-37 0.5236178740787523 1107 38-39 0.3879137114682081 1107 40-41 0.46439314955383537 1107 42-43 0.4481669601575575 1107 44-45 0.5324552694533651 1107 46-47 0.6092548663143234 1107 48-49 0.6856657361821661 1107 50-51 0.6687449918084454 1107 52-53 0.8215413949490724 1107 54-55 0.8633006567135197 1107 56-57 0.9312821032697585 1107 58-59 0.9121610544245087 1107 60-61 0.9398456707579328 1107 62-63 0.9824441636092658 1107 64-65 1.236600668196978 1107 66-67 1.3039402027898213 1107 68-69 1.5810160663468444 1107 70-71 1.6066237105184307 1107 72-73 1.7047233000461155 1107 74-75 1.7042519850682893 1107 76-77 1.5695292726561014 1107 78-79 1.4734478724500413 1107 80-81 1.6408084375965046 1107 82-83 1.9244783245939665 1107 84-85 1.9900764647158908 1107 86-87 2.183252599250185 1107 88-89 2.3075696935024546 1107 90-91 2.678367275671146 1107 92-93 2.6874423884894725 1107 94-95 2.8961781028981335 1107 96-97 3.010409425746932 1107 98-99 3.1016424112658854 1107 100-101 2.973368008709567 1108 1 0.13709269848021677 1108 2 0.15964407135949443 1108 3 0.11307679910587609 1108 4 0.07921459933288588 1108 5 -0.03635087306582818 1108 6 0.09791620464624629 1108 7 0.1600120330472805 1108 8 0.15827544985711484 1108 9 0.15316687072385093 1108 10-11 0.1683366396372321 1108 12-13 0.17686273920001838 1108 14-15 0.24839450086395232 1108 16-17 0.21152113211456225 1108 18-19 0.2210195566174562 1108 20-21 0.1807000999659394 1108 22-23 0.26304954736902886 1108 24-25 0.2025405209646962 1108 26-27 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72-73 1.9073331987568842 2316 74-75 2.2662781375385315 2316 76-77 2.267547970360031 2316 78-79 2.1912409156769392 2316 80-81 2.1996757587228117 2316 82-83 2.498596035494927 2316 84-85 2.758615545494731 2316 86-87 2.862066742486892 2316 88-89 3.2468568128851594 2316 90-91 3.4702878132992723 2316 92-93 3.6976782125889223 2316 94-95 4.213759392045176 2316 96-97 4.557950062493653 2316 98-99 5.070652326256397 2316 100-101 5.202287696347188 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 6.0 4 14.0 5 44.0 6 92.0 7 253.0 8 606.0 9 1153.0 10 2118.0 11 3410.0 12 5301.0 13 7909.0 14 10715.0 15 14868.0 16 19379.0 17 26841.0 18 27874.0 19 23724.0 20 23038.0 21 24513.0 22 26950.0 23 29365.0 24 32892.0 25 37655.0 26 42613.0 27 49192.0 28 58725.0 29 70825.0 30 84299.0 31 101446.0 32 122117.0 33 152798.0 34 197302.0 35 266989.0 36 369158.0 37 443899.0 38 227264.0 39 9808.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.17018778549411 18.754614523069534 14.80376771708486 23.271429974351502 2 15.996244645219576 19.657039560459662 39.68699014517604 24.659725649144725 3 19.666055023985272 23.169554905699012 29.562420355574464 27.601969714741255 4 13.288047043668069 16.532199386905543 36.0172318506944 34.16252171873199 5 13.977828615010926 36.868635200745814 34.16247471794159 14.99106146630167 6 33.23120572338712 35.58024677614174 16.696393143322435 14.492154357148706 7 29.325286652085737 32.30605485065147 19.813753177237047 18.554905320025746 8 26.79189965457356 34.84827159551391 19.068827649382623 19.291001100529908 9 26.197569376384855 16.764758621429994 19.859356692246248 37.1783153099389 10-11 25.395213897184156 26.357519629987316 26.573033015434028 21.67423345739451 12-13 26.137374326930683 23.496326471866368 27.212535837723053 23.153763363479893 14-15 22.782200630457787 25.012041994205923 25.54425539722876 26.661501978107527 16-17 22.411309196863098 28.190392923859807 26.530376936783057 22.867920942494035 18-19 22.451520918972452 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28.598923579384827 22.915389331698343 44-45 22.531502633627127 26.363427621723872 28.628058690273722 22.477011054375282 46-47 22.977969168525068 26.507430748855835 27.916666832328957 22.597933250290144 48-49 22.732895004168725 26.548740297325374 27.907442756058572 22.81092194244733 50-51 22.81211471093057 26.80979755935713 27.83798387138457 22.540103858327733 52-53 23.11619115625784 26.535202375040605 27.773117145371046 22.575489323330512 54-55 22.758996754476957 26.763100673238288 27.82536040493695 22.652542167347804 56-57 22.76984012124894 26.92656042010913 27.701204219539466 22.602395239102467 58-59 22.761084099322627 26.529974073189074 27.631992754329055 23.076949073159252 60-61 22.507819591381374 26.75522840124891 27.902949994771696 22.83400201259802 62-63 22.584335689581206 26.77389522801161 27.980778138303098 22.660990944104086 64-65 22.331965758002383 26.790037361484792 27.8521579368604 23.025838943652424 66-67 22.358120601290853 26.86487972612429 27.739179545757708 23.03782012682715 68-69 22.7605657434418 26.48110457776984 27.89848489125625 22.85984478753211 70-71 22.555411053862642 26.633128667514594 27.584301894474184 23.227158384148584 72-73 22.57370914567526 26.74380833634176 27.49231061612083 23.19017190186215 74-75 22.547658058721584 27.13963780392238 27.225218942594836 23.0874851947612 76-77 22.59190976944978 26.776201247079207 27.600324751099038 23.031564232371977 78-79 22.71707094229108 26.922335265750807 27.48253886337911 22.87805492857901 80-81 22.755531417441187 26.952448864493668 27.33005971201383 22.961960006051317 82-83 22.726706149515696 27.016316047808033 27.36248511739392 22.89449268528236 84-85 22.677371077494655 27.101518943252543 27.27551233818336 22.94559764106944 86-87 22.854290345782655 27.0290929462597 27.12757899658738 22.98903771137027 88-89 22.702240854149462 27.236808676356983 26.829677034077793 23.231273435415762 90-91 22.688740676506097 27.12723966133146 27.110778981779738 23.073240680382707 92-93 22.758877477628634 26.82047283728213 27.173850379916644 23.2467993051726 94-95 22.565613710319816 26.771455374645676 27.202959338713118 23.459971576321394 96-97 22.816171186438993 26.47366312778251 27.226619459456238 23.48354622632226 98-99 22.517799087691145 26.73123387526107 26.894782313787967 23.856184723259823 100-101 23.038800361170296 26.137096014284595 27.03841151864476 23.785692105900345 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 662.0 1 452.0 2 347.5 3 483.0 4 408.0 5 386.0 6 807.0 7 1757.5 8 2835.0 9 3350.0 10 2850.0 11 2171.5 12 2140.0 13 2328.5 14 2442.5 15 2620.0 16 2827.0 17 2989.0 18 3222.5 19 3616.0 20 4216.5 21 5019.5 22 5956.5 23 7327.0 24 9053.0 25 11308.0 26 14776.5 27 18432.0 28 21302.5 29 24460.5 30 27908.5 31 32605.5 32 39315.5 33 42956.0 34 44464.5 35 47472.5 36 52074.0 37 57018.5 38 60549.5 39 63352.0 40 66600.0 41 71647.5 42 75012.0 43 76822.0 44 80059.0 45 84335.0 46 86880.0 47 89974.0 48 91853.0 49 90717.5 50 91504.0 51 91123.0 52 90818.5 53 92971.5 54 94279.0 55 89602.0 56 82369.5 57 76334.0 58 71173.0 59 63742.5 60 52120.0 61 42949.0 62 35683.5 63 26703.5 64 18884.5 65 14140.0 66 10680.0 67 8165.0 68 6035.0 69 4360.0 70 3211.5 71 2133.5 72 1412.0 73 989.0 74 695.0 75 483.0 76 308.5 77 175.5 78 109.5 79 65.0 80 34.0 81 20.5 82 15.5 83 12.0 84 10.5 85 6.5 86 3.0 87 2.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.013836114405581839 3 0.002862644359775553 4 0.0018686706237423746 5 0.11927684832398137 6 1.1927684832398136E-4 7 0.0 8 1.9879474720663562E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 1.987947472066356E-5 16-17 1.987947472066356E-5 18-19 0.0 20-21 0.0 22-23 4.174689691339348E-4 24-25 5.963842416199068E-4 26-27 0.00614275768868504 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.01133130059077823 36-37 7.156610899438882E-4 38-39 0.007236128798321537 40-41 0.010734916349158323 42-43 0.006162637163405703 44-45 0.003558425974998777 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 3.975894944132712E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 5.963842416199068E-5 68-69 9.542147865918509E-4 70-71 0.0 72-73 3.975894944132712E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 5.963842416199068E-5 82-83 0.0022463806434349825 84-85 0.004731314983517928 86-87 0.00318071595530617 88-89 0.0 90-91 0.0028825238344962166 92-93 0.0 94-95 1.987947472066356E-5 96-97 1.7891527248597205E-4 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2515157.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.261849672539356 #Duplication Level Percentage of deduplicated Percentage of total 1 74.95315899778493 39.92143887021962 2 14.10125055993536 15.021173750361772 3 4.721673897484983 7.544552559917945 4 2.04486890683255 4.356540012630607 5 1.0884888363377017 2.8987464385627977 6 0.6753180813983346 2.1581214079551474 7 0.45429691759870244 1.6937685892288061 8 0.3186162306340476 1.3576071823409417 9 0.23833767086193855 1.142487467707656 >10 1.2594660274851666 12.320538902995269 >50 0.09549172277673294 3.4924509114881452 >100 0.04357567025381939 4.223157605977076 >500 0.0027656134626928264 0.9648260596897154 >1k 0.00246662822347858 1.9888514736477447 >5k 2.2423892940714364E-4 0.9157387672767666 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9674 0.3846280768953986 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8241 0.3276535023459768 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5087 0.20225377580803108 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2777 0.11041060259856543 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2620 0.10416844753627706 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.975894944132712E-5 2 0.0 0.0 0.0 0.0 3.975894944132712E-5 3 0.0 0.0 0.0 0.0 3.975894944132712E-5 4 0.0 0.0 0.0 0.0 3.975894944132712E-5 5 0.0 0.0 0.0 0.0 3.975894944132712E-5 6 0.0 0.0 0.0 0.0 7.951789888265424E-5 7 0.0 0.0 0.0 0.0 7.951789888265424E-5 8 0.0 0.0 0.0 0.0 7.951789888265424E-5 9 0.0 0.0 0.0 0.0 7.951789888265424E-5 10-11 0.0 0.0 0.0 0.0 7.951789888265424E-5 12-13 0.0 0.0 0.0 0.0 2.1867422192729916E-4 14-15 0.0 0.0 0.0 0.0 2.3855369664796272E-4 16-17 0.0 0.0 0.0 3.975894944132712E-5 2.3855369664796272E-4 18-19 0.0 0.0 0.0 7.951789888265424E-5 2.3855369664796272E-4 20-21 0.0 0.0 0.0 1.1927684832398136E-4 3.1807159553061696E-4 22-23 0.0 0.0 0.0 2.3855369664796272E-4 3.1807159553061696E-4 24-25 0.0 0.0 0.0 3.1807159553061696E-4 3.1807159553061696E-4 26-27 0.0 0.0 0.0 0.0010734916349158324 3.1807159553061696E-4 28-29 0.0 0.0 0.0 0.0032403543794681604 3.1807159553061696E-4 30-31 0.0 0.0 0.0 0.008925884149577939 3.1807159553061696E-4 32-33 0.0 0.0 0.0 0.023239105948455706 3.1807159553061696E-4 34-35 0.0 0.0 0.0 0.04671676559355937 3.578305449719441E-4 36-37 0.0 0.0 0.0 0.08484559810779208 3.578305449719441E-4 38-39 0.0 0.0 0.0 0.1427743874438057 3.578305449719441E-4 40-41 0.0 0.0 0.0 0.20452003592618673 3.578305449719441E-4 42-43 0.0 0.0 0.0 0.26902893139474 3.578305449719441E-4 44-45 0.0 0.0 0.0 0.34461069428270286 3.7771001969260764E-4 46-47 0.0 0.0 0.0 0.43418760737401285 3.9758949441327123E-4 48-49 0.0 0.0 0.0 0.5288735454685334 3.9758949441327123E-4 50-51 0.0 0.0 0.0 0.6307160944624928 3.9758949441327123E-4 52-53 0.0 0.0 0.0 0.7241297461748909 4.572279185752619E-4 54-55 0.0 0.0 0.0 0.8265487999357495 4.7710739329592545E-4 56-57 0.0 0.0 0.0 0.9474557651868254 4.7710739329592545E-4 58-59 0.0 0.0 0.0 1.0793958389078693 4.7710739329592545E-4 60-61 0.0 0.0 0.0 1.2187509566997208 4.7710739329592545E-4 62-63 0.0 0.0 0.0 1.3507307893702063 5.566252921785797E-4 64-65 0.0 0.0 0.0 1.490085907162058 5.566252921785797E-4 66-67 0.0 0.0 0.0 1.647610864848596 5.566252921785797E-4 68-69 0.0 0.0 0.0 1.8201050669997936 5.566252921785797E-4 70-71 0.0 0.0 0.0 1.9950444445416329 5.566252921785797E-4 72-73 0.0 0.0 0.0 2.1777169377498105 5.566252921785797E-4 74-75 0.0 0.0 0.0 2.361264127845697 5.963842416199067E-4 76-77 0.0 0.0 0.0 2.5615498356563826 6.361431910612339E-4 78-79 0.0 0.0 0.0 2.78616802052516 6.361431910612339E-4 80-81 0.0 0.0 0.0 3.0221373854594367 6.361431910612339E-4 82-83 0.0 0.0 0.0 3.268980823065916 6.361431910612339E-4 84-85 0.0 0.0 0.0 3.515287514854937 6.361431910612339E-4 86-87 0.0 0.0 0.0 3.764258056256528 6.957816152232247E-4 88-89 0.0 0.0 0.0 4.030603258563978 7.156610899438882E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4620 0.0 55.592907 1 GTATCAA 7785 0.0 41.23944 1 ATCAACG 10140 0.0 31.235754 3 TATCAAC 10215 0.0 31.196367 2 TCAACGC 10210 0.0 31.066942 4 CAACGCA 10380 0.0 30.649158 5 TGGTATC 1950 0.0 30.001165 2 AACGCAG 10790 0.0 29.447035 6 GTGGTAT 2215 0.0 28.773893 1 ACGCAGA 12340 0.0 25.478844 7 CGCAGAG 12400 0.0 25.240656 8 GCAGAGT 13860 0.0 22.44476 9 TAAGGTG 1465 0.0 22.397587 5 GAGTACT 8870 0.0 21.685444 12-13 GTACATG 8575 0.0 21.63207 1 TACATGG 8265 0.0 21.407585 2 CAGAGTA 13010 0.0 20.935968 10-11 GTAAGGT 1455 0.0 20.91777 4 CTATCGG 140 4.8108707E-4 20.384066 1 GTACTTT 9445 0.0 20.314976 14-15 >>END_MODULE