FastQCFastQC Report
Thu 26 May 2016
SRR938102_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938102_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2504081
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99760.39838966870480624No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82130.327984597942319No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50760.2027090976689652No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA28040.11197720840499968No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA25630.10235291909486953No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44700.059.787571
GTATCAA84100.045.566951
ATCAACG107050.035.032563
TATCAAC108850.034.6708832
TCAACGC108300.034.6282124
CAACGCA111000.033.8287285
AACGCAG114750.032.6785246
ACGCAGA130050.028.7134517
CGCAGAG131350.028.2478528
ACCGTAC1109.4704876E-525.913328
TGGTATC19000.025.266242
GCAGAGT144200.025.2344199
GTGGTAT20850.024.719981
GTACATG85850.023.5698071
GAGTACT91950.023.14609512-13
GTGTAGC16300.023.1296961
TACATGG84900.022.897522
CAGAGTA134750.022.79086710-11
ACATGGG87100.021.6647593
AGAGTAC130350.021.59227610-11
Produced by FastQC (version 0.11.5)