##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938102_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2504081 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.73580567082295 33.0 31.0 34.0 30.0 34.0 2 31.93536151586151 33.0 31.0 34.0 30.0 34.0 3 32.018317298841374 34.0 31.0 34.0 30.0 34.0 4 35.543827456060725 37.0 35.0 37.0 33.0 37.0 5 35.543414530121034 37.0 35.0 37.0 33.0 37.0 6 35.56143032114377 37.0 35.0 37.0 33.0 37.0 7 35.49829658066173 37.0 35.0 37.0 33.0 37.0 8 35.46726443753217 37.0 35.0 37.0 33.0 37.0 9 37.0834110398186 39.0 37.0 39.0 33.0 39.0 10-11 36.99107836367914 39.0 37.0 39.0 33.0 39.0 12-13 36.88342769263454 39.0 37.0 39.0 33.0 39.0 14-15 38.04045815610597 40.0 38.0 41.0 33.0 41.0 16-17 37.87503519255168 40.0 38.0 41.0 33.0 41.0 18-19 38.02183236085414 40.0 38.0 41.0 33.0 41.0 20-21 37.99129760578831 40.0 38.0 41.0 33.0 41.0 22-23 37.89600256541222 40.0 38.0 41.0 33.0 41.0 24-25 37.783431726050395 40.0 38.0 41.0 32.0 41.0 26-27 37.5982224616536 40.0 37.5 41.0 32.0 41.0 28-29 37.37781325763824 40.0 37.0 41.0 31.5 41.0 30-31 37.1541156216592 40.0 37.0 41.0 31.0 41.0 32-33 36.88838120651848 39.5 36.0 41.0 30.5 41.0 34-35 36.64201018257796 39.0 36.0 41.0 30.0 41.0 36-37 36.367854713964924 39.0 35.5 41.0 30.0 41.0 38-39 36.06244266858779 39.0 35.0 40.0 29.0 41.0 40-41 35.83351017798546 39.0 35.0 40.0 28.0 41.0 42-43 35.64006016578537 39.0 35.0 40.0 27.0 41.0 44-45 35.36262664825938 38.5 35.0 40.0 26.5 41.0 46-47 34.95754190858842 38.0 34.5 40.0 24.5 41.0 48-49 34.55303043312097 38.0 34.0 40.0 23.5 41.0 50-51 33.85511650781265 37.5 33.0 39.5 23.0 40.5 52-53 33.98207426197475 38.0 33.5 39.5 23.0 40.5 54-55 34.072107691404554 38.0 34.0 40.0 22.5 41.0 56-57 33.74340945840011 38.0 33.0 40.0 20.5 41.0 58-59 33.39844757417991 37.5 33.0 40.0 18.5 41.0 60-61 32.94996407863803 37.0 32.5 40.0 16.5 41.0 62-63 32.38185645751875 36.0 31.5 39.0 10.5 41.0 64-65 31.831342117128 35.5 31.0 39.0 7.5 40.0 66-67 31.264574508572206 35.0 30.5 38.5 4.5 40.0 68-69 30.622498034208956 35.0 29.5 38.0 2.0 40.0 70-71 30.030073108657426 34.5 29.0 37.0 2.0 39.5 72-73 29.353727375432342 34.0 28.5 36.5 2.0 39.0 74-75 28.62880673588434 34.0 26.5 36.0 2.0 39.0 76-77 27.92339225448378 33.5 26.0 35.5 2.0 38.0 78-79 27.16196540766852 33.0 25.0 35.0 2.0 37.0 80-81 26.462618621362488 32.5 23.5 35.0 2.0 36.5 82-83 25.692236792659664 32.0 20.5 35.0 2.0 36.0 84-85 24.993411954325758 31.0 18.5 34.5 2.0 35.5 86-87 24.26767165279398 31.0 11.5 34.0 2.0 35.0 88-89 23.40433576230162 30.5 4.5 34.0 2.0 35.0 90-91 22.524756986694918 30.0 2.0 34.0 2.0 35.0 92-93 21.58029033405868 29.0 2.0 34.0 2.0 35.0 94-95 20.585156989729967 28.5 2.0 34.0 2.0 35.0 96-97 19.334668287487503 26.5 2.0 33.5 2.0 35.0 98-99 17.80802278360804 24.0 2.0 33.0 2.0 34.5 100-101 14.301337696344488 2.0 2.0 29.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4403282845488796 1101 2 0.3766931850920763 1101 3 0.444018566094627 1101 4 0.3293836997330857 1101 5 0.27744267535342004 1101 6 0.2794494341869438 1101 7 0.3653644499295865 1101 8 0.22201415855152362 1101 9 0.4351864099392486 1101 10-11 0.45803254461046095 1101 12-13 0.4290840187384788 1101 14-15 0.6690538741249625 1101 16-17 0.7868026061423663 1101 18-19 0.6476217925782137 1101 20-21 0.6371827412754811 1101 22-23 0.6587290787564655 1101 24-25 0.6924055961876903 1101 26-27 0.6799542738311075 1101 28-29 0.7210759473937216 1101 30-31 0.8108435021348797 1101 32-33 0.7465375193001691 1101 34-35 0.9673822364671736 1101 36-37 1.0394094830069989 1101 38-39 0.997368060487851 1101 40-41 1.0858526690741641 1101 42-43 1.2861761053586278 1101 44-45 1.094387485716382 1101 46-47 1.2330863476147513 1101 48-49 1.1700299852956846 1101 50-51 1.1903648374083104 1101 52-53 1.2253597241768475 1101 54-55 1.2195991517446814 1101 56-57 1.3341384120157969 1101 58-59 1.3386838230552556 1101 60-61 1.3679737219939412 1101 62-63 1.34783115266017 1101 64-65 1.320661874282738 1101 66-67 1.5766716725169978 1101 68-69 1.7554873477901332 1101 70-71 1.8722900909503544 1101 72-73 2.0332917222366014 1101 74-75 2.122926802626548 1101 76-77 2.376917823462584 1101 78-79 2.5790724099843487 1101 80-81 2.8005908777293698 1101 82-83 2.9092494570538676 1101 84-85 3.020938040488989 1101 86-87 3.2994304793418507 1101 88-89 3.7001099111741063 1101 90-91 3.867994894821212 1101 92-93 4.05747720696375 1101 94-95 4.191466471576295 1101 96-97 4.515795791372753 1101 98-99 4.568597827871962 1101 100-101 4.182636695504476 1102 1 0.4180715866071729 1102 2 0.337541684968901 1102 3 0.2191703657110864 1102 4 0.2317545271557222 1102 5 0.31069971304287947 1102 6 0.3470020749608622 1102 7 0.08442003169527368 1102 8 0.2641356336686229 1102 9 0.3974051720659091 1102 10-11 0.42023802276114 1102 12-13 0.32708723587592914 1102 14-15 0.47862526060171007 1102 16-17 0.5631193701980166 1102 18-19 0.4322122596750049 1102 20-21 0.4571792310854619 1102 22-23 0.5263307092236289 1102 24-25 0.4842269742109977 1102 26-27 0.38785111449907816 1102 28-29 0.4953753585512928 1102 30-31 0.5485165762401465 1102 32-33 0.6153644959155358 1102 34-35 0.7148015222240076 1102 36-37 0.7049712985803254 1102 38-39 0.708991257691288 1102 40-41 0.9488399293386536 1102 42-43 1.0458369775281326 1102 44-45 0.9718669599607637 1102 46-47 1.034161826361192 1102 48-49 1.2805116059085577 1102 50-51 1.319027390604596 1102 52-53 1.1322336181852037 1102 54-55 1.2585771550906344 1102 56-57 1.3493847935412404 1102 58-59 1.4001517990120718 1102 60-61 1.4358651041554111 1102 62-63 1.4696311233549153 1102 64-65 1.4733142817417324 1102 66-67 1.5781156004518273 1102 68-69 1.9153742890781764 1102 70-71 2.06230146686433 1102 72-73 2.2406086972256922 1102 74-75 2.11570273496039 1102 76-77 2.4449905520234516 1102 78-79 2.7475874949454386 1102 80-81 2.7605417028655523 1102 82-83 3.0538634488644973 1102 84-85 3.334819875899722 1102 86-87 3.4806414224397386 1102 88-89 3.670878924569184 1102 90-91 3.7661172651977814 1102 92-93 3.926036088791111 1102 94-95 4.113705095259771 1102 96-97 4.533571160143978 1102 98-99 4.5762302771634005 1102 100-101 4.431670990705014 1103 1 0.5467916196104134 1103 2 0.47671952691964137 1103 3 0.44354013148798543 1103 4 0.36092687473311 1103 5 0.4513801828580526 1103 6 0.45001602548929753 1103 7 0.47384484295337614 1103 8 0.5079174644974245 1103 9 0.6176324208278245 1103 10-11 0.5588030663368855 1103 12-13 0.6588692258010624 1103 14-15 0.7716145696981371 1103 16-17 0.7990217701476965 1103 18-19 0.7849702223539694 1103 20-21 0.8245627253959142 1103 22-23 0.8148567649480469 1103 24-25 0.8437710429702108 1103 26-27 0.9140393421104278 1103 28-29 0.9911290126795649 1103 30-31 0.9818859683256633 1103 32-33 0.9370098757395269 1103 34-35 1.0133601279481539 1103 36-37 1.2548581736878575 1103 38-39 1.1909872414338594 1103 40-41 1.2155549878065557 1103 42-43 1.2521130041157207 1103 44-45 1.1831117407685667 1103 46-47 1.2187572776860378 1103 48-49 1.3034459897710065 1103 50-51 1.257045031797965 1103 52-53 1.283907228358494 1103 54-55 1.3440419476271686 1103 56-57 1.3911563682064454 1103 58-59 1.5809127512221224 1103 60-61 1.4917409849663557 1103 62-63 1.6456490161424853 1103 64-65 1.880483652737734 1103 66-67 1.8650509240926176 1103 68-69 2.1885618858540674 1103 70-71 2.302736031097627 1103 72-73 2.3877200813082915 1103 74-75 2.582192144879489 1103 76-77 2.799260161108961 1103 78-79 3.0145384229765497 1103 80-81 3.2059087621548343 1103 82-83 3.322575536300885 1103 84-85 3.333323260396714 1103 86-87 3.5781626183261714 1103 88-89 3.9833799651701973 1103 90-91 3.956336355647746 1103 92-93 4.030068209600724 1103 94-95 4.4267611230683315 1103 96-97 4.794722934345582 1103 98-99 4.953881728547447 1103 100-101 4.475474743528514 1104 1 0.17164563082750206 1104 2 0.11689534914134825 1104 3 0.08044530639823222 1104 4 0.07484121053036574 1104 5 0.044843541595128045 1104 6 0.06331952469137292 1104 7 0.14619470250084277 1104 8 0.20143865785159676 1104 9 0.19668407437021784 1104 10-11 0.22957639244643246 1104 12-13 0.1721304708324567 1104 14-15 0.3615388819668439 1104 16-17 0.4193263948812245 1104 18-19 0.36371327918769936 1104 20-21 0.2782389421145197 1104 22-23 0.1933265822995125 1104 24-25 0.19651982842248117 1104 26-27 0.29445546882806894 1104 28-29 0.3059361911521208 1104 30-31 0.29830769652539146 1104 32-33 0.2867467531604717 1104 34-35 0.29351099237209155 1104 36-37 0.41818726636990533 1104 38-39 0.481098996859906 1104 40-41 0.36214694173164474 1104 42-43 0.3994647644776492 1104 44-45 0.4856454745124523 1104 46-47 0.5427108014221247 1104 48-49 0.6784342005665565 1104 50-51 0.6132279553794078 1104 52-53 0.5834810927620779 1104 54-55 0.692919926972067 1104 56-57 0.8643075197260757 1104 58-59 0.8552027710529089 1104 60-61 0.9378517523843612 1104 62-63 0.9917657249040559 1104 64-65 0.9773748135557732 1104 66-67 1.2232894279044366 1104 68-69 1.2816717761968128 1104 70-71 1.4243067229068416 1104 72-73 1.5980544459979313 1104 74-75 1.6867115961320103 1104 76-77 1.8381970470056643 1104 78-79 1.9976578402429261 1104 80-81 2.131121269056969 1104 82-83 2.2698730143875068 1104 84-85 2.702672596025703 1104 86-87 2.786935718415375 1104 88-89 2.95440739797381 1104 90-91 2.942661501698854 1104 92-93 3.2724681717090824 1104 94-95 3.5291530124280506 1104 96-97 3.696465264507289 1104 98-99 4.169948451082131 1104 100-101 4.266963810417655 1105 1 0.3835081288490976 1105 2 0.34407676555538913 1105 3 0.2693339923741007 1105 4 0.20847160852396485 1105 5 0.19700700020695905 1105 6 0.2861606316224794 1105 7 0.2505082274652324 1105 8 0.28240703994492833 1105 9 0.39540085243613277 1105 10-11 0.3583673149295947 1105 12-13 0.23340918809751088 1105 14-15 0.4087344211270292 1105 16-17 0.5195568026146375 1105 18-19 0.4906231979819111 1105 20-21 0.5071552648436253 1105 22-23 0.4273487038768735 1105 24-25 0.4498846335651123 1105 26-27 0.43369083169741884 1105 28-29 0.5521985333365222 1105 30-31 0.4207768638921081 1105 32-33 0.5615117798841212 1105 34-35 0.6194283621323677 1105 36-37 0.6491474304859537 1105 38-39 0.7663982529807853 1105 40-41 0.8924971493116587 1105 42-43 0.6921936808003792 1105 44-45 0.8600976093603236 1105 46-47 0.8585581053168383 1105 48-49 0.8612339741156205 1105 50-51 0.9057666407465419 1105 52-53 0.8149936875731854 1105 54-55 0.928538466728817 1105 56-57 0.9904382525261539 1105 58-59 1.0197479821027429 1105 60-61 1.1211141676174492 1105 62-63 1.1235942552862213 1105 64-65 1.0954854092678197 1105 66-67 1.4291832394631605 1105 68-69 1.6013252210511375 1105 70-71 1.6031375177983023 1105 72-73 1.6708337659146544 1105 74-75 1.803327737456872 1105 76-77 1.900819555741073 1105 78-79 2.1512335734691206 1105 80-81 2.3338192005616527 1105 82-83 2.3422657850024535 1105 84-85 2.693419762345389 1105 86-87 2.8832671524828477 1105 88-89 3.169433817248926 1105 90-91 3.339533496049757 1105 92-93 3.5955533830024073 1105 94-95 3.767107588319547 1105 96-97 3.9833076741399758 1105 98-99 4.195332869797205 1105 100-101 4.116973550242582 1106 1 0.1704081169357181 1106 2 0.14646181232633282 1106 3 0.21180467204194997 1106 4 0.18300378993921385 1106 5 0.19748057276850517 1106 6 0.02798234379390152 1106 7 0.17071039320595816 1106 8 0.15791873845026316 1106 9 0.25413121016819673 1106 10-11 0.19943249610346214 1106 12-13 0.3204687281910381 1106 14-15 0.4262202732052245 1106 16-17 0.23418333365587074 1106 18-19 0.3118582103537406 1106 20-21 0.1881931512722801 1106 22-23 0.11651100282294635 1106 24-25 0.17570693766325007 1106 26-27 0.26340048509282354 1106 28-29 0.2637519885606068 1106 30-31 0.29793476112390493 1106 32-33 0.3428986317508702 1106 34-35 0.2695468878169365 1106 36-37 0.30931935432263913 1106 38-39 0.20391513287204788 1106 40-41 0.46317087606107776 1106 42-43 0.43885426914684444 1106 44-45 0.4921408511976324 1106 46-47 0.6727225873435358 1106 48-49 0.6585590298309612 1106 50-51 0.6055548507389332 1106 52-53 0.6198638597683441 1106 54-55 0.17445314320562488 1106 56-57 0.6134963243420302 1106 58-59 0.9160820595547037 1106 60-61 1.0168568641212161 1106 62-63 0.5983475515820302 1106 64-65 0.7497448490643812 1106 66-67 1.023249376826346 1106 68-69 0.7481101826195662 1106 70-71 1.0237348990995336 1106 72-73 1.4349022391741002 1106 74-75 1.4100445151799654 1106 76-77 1.6254249483244578 1106 78-79 1.8254663297830582 1106 80-81 2.0888825442053616 1106 82-83 2.144976500622519 1106 84-85 2.2987141071062105 1106 86-87 2.167897126554088 1106 88-89 2.3129580360335247 1106 90-91 2.4611996200649493 1106 92-93 2.696736831009847 1106 94-95 2.940155321689307 1106 96-97 3.0787132094344685 1106 98-99 3.0766475084755314 1106 100-101 3.2756203583603973 1107 1 0.3030272415846973 1107 2 0.2744756120871763 1107 3 0.03559244517159499 1107 4 0.13918759758928445 1107 5 0.07807135626374162 1107 6 0.24605955009296565 1107 7 0.21045826833253756 1107 8 0.2471396269524888 1107 9 0.20232655965480006 1107 10-11 0.2833471873991158 1107 12-13 0.20936038610649632 1107 14-15 0.32424226244555854 1107 16-17 0.45238694768593035 1107 18-19 0.3594633318185103 1107 20-21 0.479262640244265 1107 22-23 0.44278789730627466 1107 24-25 0.4245916746803715 1107 26-27 0.4811706046772457 1107 28-29 0.5375476809394328 1107 30-31 0.5869572555447746 1107 32-33 0.5415470298481537 1107 34-35 0.6004205498954462 1107 36-37 0.6091010682883677 1107 38-39 0.6500158972484869 1107 40-41 0.6132980719637828 1107 42-43 0.7055469579909115 1107 44-45 0.540915919749807 1107 46-47 0.5883078198579383 1107 48-49 0.6364718310157684 1107 50-51 0.5000184323263497 1107 52-53 0.4692977648139234 1107 54-55 0.545875387813588 1107 56-57 0.587218153771154 1107 58-59 0.7336916923642676 1107 60-61 0.8324202530194 1107 62-63 0.8621589098120808 1107 64-65 1.0975521928731418 1107 66-67 1.087463077953128 1107 68-69 1.2135969687330324 1107 70-71 1.3469531618639756 1107 72-73 1.7309273015334945 1107 74-75 1.8293401598931744 1107 76-77 2.0182989001499045 1107 78-79 2.078463596703788 1107 80-81 2.1371926665070937 1107 82-83 2.1738145028098828 1107 84-85 2.314251373741701 1107 86-87 2.382248607603813 1107 88-89 2.637248212336278 1107 90-91 3.008691920377892 1107 92-93 3.1639730580843697 1107 94-95 3.4728371998199457 1107 96-97 3.629892652114492 1107 98-99 3.5877186938933043 1107 100-101 3.5506921382848393 1108 1 0.3692510648866154 1108 2 0.11638984132486385 1108 3 0.14097102253430194 1108 4 0.1247764479631357 1108 5 0.1338961192052679 1108 6 0.1611618218436206 1108 7 0.13296020204210635 1108 8 0.11338135497265966 1108 9 0.20322179556736586 1108 10-11 0.245934562424182 1108 12-13 0.2502279055152101 1108 14-15 0.3383060605376329 1108 16-17 0.3489743083872199 1108 18-19 0.301211405270827 1108 20-21 0.21590249757947078 1108 22-23 0.2536496155862693 1108 24-25 0.12894001455489246 1108 26-27 0.19822878784967912 1108 28-29 0.38039725265857527 1108 30-31 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-2.2422166679299487 2316 68-69 -2.2938167761706545 2316 70-71 -2.422558748772328 2316 72-73 -2.5237543049467597 2316 74-75 -2.7361502341093846 2316 76-77 -2.9893276720547135 2316 78-79 -3.6214379636336567 2316 80-81 -3.641579952256855 2316 82-83 -3.4300820567426697 2316 84-85 -3.457212193889287 2316 86-87 -3.2137321272413892 2316 88-89 -3.4121609933357 2316 90-91 -3.6260754799581996 2316 92-93 -3.9781505493602687 2316 94-95 -4.309398131012998 2316 96-97 -4.3413673442956515 2316 98-99 -4.245998954386911 2316 100-101 -3.8682393598429936 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7953.0 3 2571.0 4 2805.0 5 3123.0 6 3683.0 7 4436.0 8 5545.0 9 6958.0 10 8576.0 11 10431.0 12 12535.0 13 15434.0 14 17756.0 15 20979.0 16 24756.0 17 30290.0 18 29826.0 19 26234.0 20 27107.0 21 28553.0 22 31513.0 23 35094.0 24 39091.0 25 44204.0 26 49708.0 27 56282.0 28 64835.0 29 75167.0 30 88171.0 31 104297.0 32 126986.0 33 157713.0 34 201931.0 35 265096.0 36 337885.0 37 354412.0 38 172682.0 39 9463.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.80053984051917 18.352250753032592 14.427655111875689 23.41955429457255 2 15.680990016655555 19.388838262149385 40.18886415036901 24.741307570826056 3 19.65383003317539 23.187330462102025 29.26514298819822 27.893696516524365 4 13.011856203968902 16.182727470335514 36.35002173690671 34.45539458878887 5 14.03106147627144 36.87577088144473 34.024418806349786 15.068748835934045 6 33.20582328314066 35.30064647635415 16.921053946303495 14.572476294201698 7 29.29299386469732 32.27953902925096 19.908130274898 18.519336831153723 8 26.834505845113384 34.62921553908042 19.220057602163138 19.31622101364306 9 26.34245114572958 16.733329258065535 19.971728828484363 36.952490767720526 10-11 25.412725930963227 26.263409422445406 26.579953500284283 21.74391114630709 12-13 26.20498113605572 23.4897757541882 27.207988419939404 23.097254689816666 14-15 22.88549661016272 25.00477413735932 25.532326303299545 26.577402949178406 16-17 22.47412647450281 28.15446671003973 26.488525480211557 22.8828813352459 18-19 22.471635823025597 26.469789159984064 28.892785268886843 22.165789748103496 20-21 22.895651753495343 25.756237196261456 29.28258707541135 22.065523974831848 22-23 22.69531598816007 25.615803992710017 29.165585415079747 22.523294604050164 24-25 22.48376094675029 25.850135167265748 29.222763246491944 22.443340639492018 26-27 22.119795873639525 26.21990433799483 28.95682623187158 22.703473556494068 28-29 22.233393428786886 26.585096660457356 28.874004304898694 22.30750560585706 30-31 22.355728989165016 26.165512876806247 28.821260524245567 22.65749760978317 32-33 22.121687560004172 26.055783752858126 28.86888317030577 22.95364551683193 34-35 22.71630874416609 26.295407226160222 28.67737750532099 22.3109065243527 36-37 22.84100994882656 25.84503498559804 28.64947620985326 22.66447885572214 38-39 22.39408011024896 26.20410038247601 28.617354224712145 22.784465282562888 40-41 22.808059960179136 25.989614779376474 28.701739571389457 22.500585689054933 42-43 22.42608399193678 26.045005994687763 28.568726603691317 22.96018340968413 44-45 22.528129357299452 26.351609077921047 28.589595962137654 22.53066560264185 46-47 22.965320363164203 26.52432076482028 27.951448148916736 22.55891072309878 48-49 22.6928892893244 26.594425828542988 27.87996495146273 22.83271993066989 50-51 22.793230420450612 26.84048742826034 27.820767984291866 22.545514166997183 52-53 23.042571938451903 26.551383809674444 27.817366360076495 22.588677891797154 54-55 22.709204561340442 26.761588081401154 27.874782817087052 22.65442454017135 56-57 22.759931037471464 26.897223425451788 27.73415196879312 22.608693568283627 58-59 22.74321008413766 26.55529658271007 27.674882059292777 23.02661127385949 60-61 22.440922316243427 26.798672248691553 27.968053605332894 22.79235182973213 62-63 22.538924982685362 26.764435454945595 28.09752274867774 22.599116813691307 64-65 22.29281745336724 26.724699701214018 27.997630641729828 22.98485220368892 66-67 22.29879998586175 26.79358610585198 27.826597128297255 23.08101677998902 68-69 22.72393863683248 26.415382209761802 27.985087965069987 22.875591188335733 70-71 22.479465999145628 26.559065246866354 27.70713592852508 23.25433282546294 72-73 22.517493466536244 26.65830953140796 27.64808485534514 23.17611214671066 74-75 22.468886297189783 26.99652916460307 27.470104484527024 23.064480053680125 76-77 22.65118335467411 26.631990235696424 27.594921188235226 23.12190522139424 78-79 22.772211187561584 26.792318012307252 27.41766574390898 23.017805056222183 80-81 22.789189823940415 26.768753986693778 27.30291603308941 23.1391401562764 82-83 22.751862584806595 26.825634662779635 27.344284674971536 23.078218077442227 84-85 22.792858513650145 26.861491965398503 27.245953043906486 23.099696477044866 86-87 22.889753732550314 26.840835707881638 27.076220504515803 23.193190055052245 88-89 22.833088228391603 26.874154008459467 26.902955420327846 23.38980234282109 90-91 22.83055763061915 26.82321523447331 26.972579865535877 23.373647269371663 92-93 22.95495136378515 26.442675330324246 27.10289444742122 23.499478858469384 94-95 22.768105836368207 26.366472846114096 27.19571135325309 23.669709964264605 96-97 23.059672636167246 26.03483593460393 27.149183987166214 23.75630744206261 98-99 22.8473279088625 26.283957866481323 26.772539714875755 24.096174509780425 100-101 23.705253416079263 25.149614383138 26.885115231121155 24.260016969661578 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 668.0 1 461.0 2 347.0 3 456.0 4 403.5 5 408.5 6 760.5 7 1697.5 8 2901.0 9 3403.0 10 2876.0 11 2230.5 12 2158.5 13 2291.5 14 2448.5 15 2611.5 16 2786.5 17 3032.0 18 3310.0 19 3705.5 20 4229.0 21 4974.0 22 6029.0 23 7494.0 24 9246.0 25 11336.0 26 14564.5 27 18458.5 28 21443.5 29 24391.5 30 27659.5 31 32137.0 32 38834.5 33 42553.5 34 43876.0 35 47121.0 36 52010.0 37 56431.0 38 59743.0 39 62927.0 40 66520.0 41 71429.5 42 73986.0 43 75383.0 44 78695.5 45 83018.0 46 85843.5 47 88586.5 48 90456.0 49 89812.5 50 90077.0 51 89767.5 52 90026.0 53 91726.0 54 93174.5 55 89498.5 56 82263.5 57 76189.5 58 71343.0 59 64169.5 60 53189.0 61 43934.0 62 36127.0 63 27496.5 64 19925.0 65 14936.5 66 11304.5 67 8646.0 68 6539.5 69 4750.5 70 3478.0 71 2382.5 72 1622.0 73 1171.5 74 830.5 75 580.0 76 386.5 77 244.5 78 155.0 79 95.5 80 63.0 81 51.5 82 35.0 83 17.5 84 16.0 85 13.5 86 5.5 87 8.5 88 11.0 89 4.5 90 3.0 91 3.0 92 2.0 93 2.5 94 1.5 95 0.5 96 0.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4589707761050861 2 0.04041402814046351 3 0.05279381936926161 4 0.05690710484205582 5 0.08286473161211638 6 0.0821059702142223 7 0.04748248958400307 8 0.041931550936251664 9 0.04704320666943282 10-11 0.050717209227656775 12-13 0.04624451046112326 14-15 0.04037409333004803 16-17 0.03959536452694622 18-19 0.047442554773587595 20-21 0.05566912571917602 22-23 0.047702131041288204 24-25 0.06493400173556686 26-27 0.05652772414310879 28-29 0.044667085449711895 30-31 0.046723728186108995 32-33 0.045126335769489885 34-35 0.05023799150267104 36-37 0.0448268246913738 38-39 0.02619723563255342 40-41 0.02473961505238848 42-43 0.024539941000311094 44-45 0.015175227957881553 46-47 0.02777466064396479 48-49 0.02777466064396479 50-51 0.01637327227034589 52-53 0.01649307670159232 54-55 0.01721190328907092 56-57 0.016073761192229804 58-59 0.01543480422558216 60-61 0.01795069728175726 62-63 0.01673268556408519 64-65 0.01795069728175726 66-67 0.009404647852845015 68-69 0.013238389652730883 70-71 0.019548089698376372 72-73 0.017251838099486397 74-75 0.014416466559987476 76-77 0.010662594380932566 78-79 0.014136922887079132 80-81 0.01757131658281022 82-83 0.014755912448519037 84-85 0.012639367496498716 86-87 0.0038337417998858666 88-89 0.029212313818921993 90-91 0.03238713124695247 92-93 0.017671153608848918 94-95 0.03276651194589952 96-97 0.02751508437626419 98-99 0.02440016916385692 100-101 0.14921641911743272 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2504081.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.354008441342565 #Duplication Level Percentage of deduplicated Percentage of total 1 76.62617264940125 42.4156580766273 2 13.22900343314701 14.645567354179386 3 4.325524618698507 7.183053787700168 4 1.960180792024788 4.340154564331907 5 1.0375504591877673 2.8716288438099262 6 0.6096434745839846 2.0247726022998775 7 0.4133660149403805 1.6017026116261772 8 0.28025293632894893 1.2410498722610943 9 0.2237989754361438 1.114935333791051 >10 1.1688410420978117 11.877713622044766 >50 0.08191057494556211 3.1147694237075267 >100 0.038529981687404985 3.7915645375168237 >500 0.0029753742825235434 1.1119987220006688 >1k 0.0020319629246460277 1.7308648556278012 >5k 2.1771031335493158E-4 0.9345657924754176 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9976 0.39838966870480624 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8213 0.327984597942319 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5076 0.2027090976689652 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2804 0.11197720840499968 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2563 0.10235291909486953 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 3.9934810415477775E-5 7 0.0 0.0 0.0 0.0 3.9934810415477775E-5 8 0.0 0.0 0.0 0.0 3.9934810415477775E-5 9 0.0 0.0 0.0 0.0 3.9934810415477775E-5 10-11 0.0 0.0 0.0 1.9967405207738888E-5 3.9934810415477775E-5 12-13 0.0 0.0 0.0 3.9934810415477775E-5 1.1980443124643332E-4 14-15 0.0 0.0 0.0 3.9934810415477775E-5 1.597392416619111E-4 16-17 0.0 0.0 0.0 5.9902215623216666E-5 2.3960886249286664E-4 18-19 0.0 0.0 0.0 7.986962083095555E-5 2.7954367290834443E-4 20-21 0.0 0.0 0.0 9.983702603869443E-5 2.7954367290834443E-4 22-23 0.0 0.0 0.0 1.9967405207738887E-4 2.7954367290834443E-4 24-25 0.0 0.0 0.0 3.793806989470388E-4 2.7954367290834443E-4 26-27 0.0 0.0 0.0 7.188265874785999E-4 3.5941329373929997E-4 28-29 0.0 0.0 0.0 0.0030350455915763106 3.5941329373929997E-4 30-31 0.0 0.0 0.0 0.00950448487888371 3.5941329373929997E-4 32-33 0.0 0.0 0.0 0.02388101662845571 3.5941329373929997E-4 34-35 0.0 0.0 0.0 0.046823565212147686 3.5941329373929997E-4 36-37 0.0 0.0 0.0 0.08719765854219572 3.5941329373929997E-4 38-39 0.0 0.0 0.0 0.1456821883956629 3.5941329373929997E-4 40-41 0.0 0.0 0.0 0.20786068821256182 3.5941329373929997E-4 42-43 0.0 0.0 0.0 0.26979957916696784 3.5941329373929997E-4 44-45 0.0 0.0 0.0 0.34928981929897635 3.5941329373929997E-4 46-47 0.0 0.0 0.0 0.4386040227931924 3.5941329373929997E-4 48-49 0.0 0.0 0.0 0.5340681870913919 3.9934810415477774E-4 50-51 0.0 0.0 0.0 0.6364410735914693 4.392829145702555E-4 52-53 0.0 0.0 0.0 0.7318852704844612 4.392829145702555E-4 54-55 0.0 0.0 0.0 0.8357357449699111 4.392829145702555E-4 56-57 0.0 0.0 0.0 0.955799752484045 4.392829145702555E-4 58-59 0.0 0.0 0.0 1.0841702005645983 4.392829145702555E-4 60-61 0.0 0.0 0.0 1.2216058506094651 4.392829145702555E-4 62-63 0.0 0.0 0.0 1.354049649352397 4.392829145702555E-4 64-65 0.0 0.0 0.0 1.4951193671450724 5.19152535401211E-4 66-67 0.0 0.0 0.0 1.6582930025027145 5.19152535401211E-4 68-69 0.0 0.0 0.0 1.8329478958548067 5.19152535401211E-4 70-71 0.0 0.0 0.0 2.0160889364201875 5.391199406089499E-4 72-73 0.0 0.0 0.0 2.2005278583240715 5.990221562321666E-4 74-75 0.0 0.0 0.0 2.383828638131115 6.389569666476444E-4 76-77 0.0 0.0 0.0 2.5800682965127724 6.389569666476444E-4 78-79 0.0 3.9934810415477775E-5 0.0 2.7996099167718618 6.389569666476444E-4 80-81 0.0 3.9934810415477775E-5 0.0 3.0318508067430727 6.389569666476444E-4 82-83 0.0 3.9934810415477775E-5 0.0 3.2694429613099576 6.389569666476444E-4 84-85 0.0 3.9934810415477775E-5 0.0 3.5057572019435472 6.389569666476444E-4 86-87 0.0 3.9934810415477775E-5 0.0 3.7531932872778473 6.389569666476444E-4 88-89 0.0 3.9934810415477775E-5 0.0 4.020157494905317 6.389569666476444E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4470 0.0 59.78757 1 GTATCAA 8410 0.0 45.56695 1 ATCAACG 10705 0.0 35.03256 3 TATCAAC 10885 0.0 34.670883 2 TCAACGC 10830 0.0 34.628212 4 CAACGCA 11100 0.0 33.828728 5 AACGCAG 11475 0.0 32.678524 6 ACGCAGA 13005 0.0 28.713451 7 CGCAGAG 13135 0.0 28.247852 8 ACCGTAC 110 9.4704876E-5 25.91332 8 TGGTATC 1900 0.0 25.26624 2 GCAGAGT 14420 0.0 25.234419 9 GTGGTAT 2085 0.0 24.71998 1 GTACATG 8585 0.0 23.569807 1 GAGTACT 9195 0.0 23.146095 12-13 GTGTAGC 1630 0.0 23.129696 1 TACATGG 8490 0.0 22.89752 2 CAGAGTA 13475 0.0 22.790867 10-11 ACATGGG 8710 0.0 21.664759 3 AGAGTAC 13035 0.0 21.592276 10-11 >>END_MODULE