FastQCFastQC Report
Thu 26 May 2016
SRR938102_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938102_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2504081
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96090.3837335932823259No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84930.3391663448586527No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52460.2094980154395964No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA27880.11133825143835203No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA26090.10418992037398152No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46100.058.019791
GTATCAA81650.042.5915571
TGGTATC18500.033.3841482
TCAACGC105800.033.040614
ATCAACG105050.033.0078623
TATCAAC105600.032.9318282
CAACGCA107700.032.501825
AACGCAG110800.031.635346
GTGGTAT20600.031.1340221
ACGCAGA126550.027.660587
CGCAGAG126150.027.4470838
TAAGGTG14050.025.0156215
GCAGAGT142700.024.0308519
TACATGG85200.023.6983432
GTACATG87150.023.386141
GTAAGGT13900.022.8936964
GAGTACT92750.022.48097812-13
ACATGGG88600.022.355943
CAGAGTA134750.021.87123310-11
GTATACG1100.00287541921.594641
Produced by FastQC (version 0.11.5)