##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938102_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2504081 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.471327405143846 33.0 31.0 34.0 31.0 34.0 2 32.703568295115055 34.0 31.0 34.0 31.0 34.0 3 32.67174065056202 34.0 31.0 34.0 31.0 34.0 4 36.202667565466136 37.0 35.0 37.0 35.0 37.0 5 36.16277109246865 37.0 35.0 37.0 35.0 37.0 6 36.22007954215538 37.0 37.0 37.0 35.0 37.0 7 36.18015311805009 37.0 37.0 37.0 35.0 37.0 8 36.169833963038734 37.0 37.0 37.0 35.0 37.0 9 37.873479731686 39.0 38.0 39.0 35.0 39.0 10-11 37.84100973570743 39.0 38.0 39.0 35.0 39.0 12-13 37.78070937002437 39.0 38.0 39.0 35.0 39.0 14-15 39.054621435967924 40.0 38.0 41.0 36.0 41.0 16-17 38.98333720035414 40.0 38.0 41.0 36.0 41.0 18-19 38.97102529830305 40.0 38.0 41.0 35.5 41.0 20-21 38.972500290525744 40.0 38.5 41.0 35.5 41.0 22-23 39.00453799218156 40.0 38.5 41.0 35.0 41.0 24-25 38.95853888113044 40.0 38.0 41.0 35.0 41.0 26-27 38.785941628885006 40.0 38.0 41.0 35.0 41.0 28-29 38.705524302129206 40.0 38.0 41.0 34.5 41.0 30-31 38.4911955723477 40.0 38.0 41.0 34.0 41.0 32-33 38.29716051517502 40.0 38.0 41.0 34.0 41.0 34-35 38.0960504073151 40.0 38.0 41.0 33.5 41.0 36-37 38.022234304720975 40.0 38.0 41.0 33.0 41.0 38-39 37.976062275940755 40.0 38.0 41.0 33.0 41.0 40-41 37.854727742433255 40.0 37.5 41.0 33.0 41.0 42-43 37.67385440007732 40.0 37.0 41.0 33.0 41.0 44-45 37.42804146511235 40.0 37.0 41.0 32.5 41.0 46-47 37.07942514639103 40.0 36.5 41.0 31.5 41.0 48-49 36.70798288873243 40.0 36.0 41.0 31.0 41.0 50-51 36.40317984921414 39.0 35.5 41.0 30.0 41.0 52-53 35.93016799376697 39.0 35.0 40.0 29.0 41.0 54-55 35.61277610428736 38.5 35.0 40.0 28.0 41.0 56-57 35.33090602899826 38.0 35.0 40.0 28.0 41.0 58-59 34.93683371264747 38.0 34.0 40.0 26.5 41.0 60-61 34.51534754666483 37.5 34.0 40.0 26.0 41.0 62-63 34.07230836382689 37.0 33.5 40.0 26.0 41.0 64-65 33.6252423543807 36.0 33.0 39.0 24.5 41.0 66-67 33.10356953309417 36.0 32.0 39.0 23.0 40.0 68-69 32.57103304565627 35.0 32.0 38.5 22.0 40.0 70-71 31.9696605261571 35.0 31.0 37.5 20.0 40.0 72-73 31.49193756112522 35.0 31.0 37.0 18.5 39.0 74-75 30.93604220470504 34.0 30.0 36.5 17.5 39.0 76-77 29.74111460451958 33.5 28.5 35.0 16.0 37.5 78-79 30.434216984195 34.0 30.0 35.5 16.0 37.5 80-81 30.247818061795925 34.0 30.0 35.0 11.5 37.0 82-83 29.783292553236095 34.0 29.5 35.0 7.0 37.0 84-85 29.269984477339193 34.0 29.0 35.0 3.5 36.0 86-87 28.722904929992282 34.0 29.0 35.0 2.0 36.0 88-89 28.078555765568286 33.0 27.0 35.0 2.0 35.0 90-91 27.539130123985608 33.0 26.5 35.0 2.0 35.0 92-93 26.89073336685195 33.0 25.5 35.0 2.0 35.0 94-95 26.121009863498827 32.0 24.5 34.0 2.0 35.0 96-97 25.281823351560913 32.0 21.5 34.0 2.0 35.0 98-99 24.233890596989475 31.5 9.5 34.0 2.0 35.0 100-101 20.741559677981662 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.0020965919561959367 1101 2 0.0805222142836115 1101 3 0.13182185829416682 1101 4 0.05377140177594697 1101 5 0.03397655909900976 1101 6 0.07860623560543445 1101 7 0.07475304490010615 1101 8 0.10694259299190634 1101 9 0.09347335074584606 1101 10-11 0.13743462173464138 1101 12-13 0.08677588052895402 1101 14-15 0.31499160827682005 1101 16-17 0.2569341091625219 1101 18-19 0.3473817991023296 1101 20-21 0.27056010990039425 1101 22-23 0.18317036172160073 1101 24-25 0.1666990527305714 1101 26-27 0.24977116208119554 1101 28-29 0.1604959109555395 1101 30-31 0.19444959813358764 1101 32-33 0.19391139299284532 1101 34-35 0.16818894125621142 1101 36-37 0.32091576404712185 1101 38-39 0.4466326441434134 1101 40-41 0.4319627733889675 1101 42-43 0.4663143155947651 1101 44-45 0.390589837014808 1101 46-47 0.5126216309759712 1101 48-49 0.46163549906799517 1101 50-51 0.4352462489916462 1101 52-53 0.3655919881926053 1101 54-55 0.3808914648746722 1101 56-57 0.43859902275858786 1101 58-59 0.4943364443427569 1101 60-61 0.5813753124541492 1101 62-63 0.4295746121899171 1101 64-65 0.3695031268139459 1101 66-67 0.3863944363773797 1101 68-69 0.4071856445833433 1101 70-71 0.5232606552311587 1101 72-73 0.3430902604963819 1101 74-75 0.4748020110155373 1101 76-77 0.6155910600030694 1101 78-79 0.6622653500789255 1101 80-81 0.8738272497522388 1101 82-83 1.0179446737507796 1101 84-85 1.0170849669494366 1101 86-87 1.2167923424308462 1101 88-89 1.283448668075188 1101 90-91 1.537643371850045 1101 92-93 1.8848598265189764 1101 94-95 1.8474195104974314 1101 96-97 1.8696253936232452 1101 98-99 2.2268196613895377 1101 100-101 2.8174550372034766 1102 1 0.06688065460566861 1102 2 0.07821804826772905 1102 3 0.14818852179414677 1102 4 0.04543993356651299 1102 5 -0.01960858474632232 1102 6 0.03376194795663423 1102 7 0.07130726203917703 1102 8 0.03629156545623857 1102 9 0.07705838187865766 1102 10-11 0.11310864262510023 1102 12-13 0.09289918963982302 1102 14-15 0.14129577268253257 1102 16-17 0.2132547859529268 1102 18-19 0.24805569922937565 1102 20-21 0.23472135160595542 1102 22-23 0.21406748095052563 1102 24-25 0.17115985211999174 1102 26-27 0.23376598374583324 1102 28-29 0.1142527803812996 1102 30-31 0.11688707891208594 1102 32-33 0.21865219444330108 1102 34-35 0.11473080027216298 1102 36-37 0.19941347517780628 1102 38-39 0.260050949301295 1102 40-41 0.25634539054155425 1102 42-43 0.21650391416105919 1102 44-45 0.22725671032110029 1102 46-47 0.24245997706460543 1102 48-49 0.3678266772197958 1102 50-51 0.42265505248379753 1102 52-53 0.41546969510461906 1102 54-55 0.5308601066404677 1102 56-57 0.43721889816294635 1102 58-59 0.33770206444680184 1102 60-61 0.4354765991481102 1102 62-63 0.42211787367170217 1102 64-65 0.44902362358634207 1102 66-67 0.42150317980986074 1102 68-69 0.5461412664424827 1102 70-71 0.6529551318344566 1102 72-73 0.6575193554631227 1102 74-75 0.7711161907340447 1102 76-77 0.890070408653493 1102 78-79 0.9285386232023036 1102 80-81 1.0171098248791637 1102 82-83 0.9767975581447637 1102 84-85 0.9479541495743184 1102 86-87 1.1014856194508518 1102 88-89 1.2546502157791508 1102 90-91 1.2956654844369737 1102 92-93 1.3485213089462889 1102 94-95 1.4298638908925376 1102 96-97 1.6420801435705137 1102 98-99 1.851735126996921 1102 100-101 2.0862796388049603 1103 1 0.27463780330339205 1103 2 0.2451628251906186 1103 3 0.41393119642549436 1103 4 0.18182572737396896 1103 5 0.12184470475535392 1103 6 0.18298968325733966 1103 7 0.17258780252271322 1103 8 0.09287112379742268 1103 9 0.12755204299976697 1103 10-11 0.24927359777288416 1103 12-13 0.3094743913314275 1103 14-15 0.4337890619622087 1103 16-17 0.36677627228267085 1103 18-19 0.4608708625898572 1103 20-21 0.416520129894117 1103 22-23 0.4938973496220527 1103 24-25 0.468752086368994 1103 26-27 0.47428078987955047 1103 28-29 0.43794074437372643 1103 30-31 0.4657888054772883 1103 32-33 0.543287799092603 1103 34-35 0.5482056399271116 1103 36-37 0.6050635686931471 1103 38-39 0.6190599178945675 1103 40-41 0.8086422975978493 1103 42-43 0.7526160067773233 1103 44-45 0.6441836814044137 1103 46-47 0.6274266223334308 1103 48-49 0.6600355355556147 1103 50-51 0.7196430444105815 1103 52-53 0.7371148436839405 1103 54-55 0.7867857513043575 1103 56-57 0.7532598321694124 1103 58-59 0.8703716176636078 1103 60-61 1.0245390962575627 1103 62-63 0.9898457375444423 1103 64-65 0.9429018834424525 1103 66-67 0.8421520338537718 1103 68-69 0.867261952110681 1103 70-71 1.032859831281133 1103 72-73 0.7590942704113388 1103 74-75 0.7820538323714068 1103 76-77 0.6684822850097447 1103 78-79 1.0576536221015793 1103 80-81 1.2050654925010207 1103 82-83 1.2946630125960361 1103 84-85 1.568718672202074 1103 86-87 1.6442129283363833 1103 88-89 1.8540012507377952 1103 90-91 1.6814613730917394 1103 92-93 2.0022273285225403 1103 94-95 2.035204162799271 1103 96-97 2.0557259840338915 1103 98-99 2.3560277218026116 1103 100-101 2.7228162041371284 1104 1 0.017166563768775234 1104 2 -0.0326504529228373 1104 3 0.047654220959920224 1104 4 -0.023453717280752073 1104 5 -0.07840287892514652 1104 6 -0.002739624974999799 1104 7 -0.047245856476571646 1104 8 -0.010302176884835035 1104 9 0.016594128070664738 1104 10-11 -0.013211117809177608 1104 12-13 -0.07342359663049791 1104 14-15 0.062007099299613344 1104 16-17 0.03859972048059035 1104 18-19 0.03536970195047218 1104 20-21 0.010884142303630995 1104 22-23 0.06421699390591584 1104 24-25 0.019398093225220236 1104 26-27 0.018746552991117937 1104 28-29 0.022898945152086014 1104 30-31 -0.01200895451154338 1104 32-33 -0.029240321535773717 1104 34-35 0.041269017443816836 1104 36-37 -0.006433845160479734 1104 38-39 -0.010249373909736903 1104 40-41 -0.09785944346510433 1104 42-43 -0.05180275324349992 1104 44-45 -0.1334684948931084 1104 46-47 -0.04328864064581239 1104 48-49 0.013435064319821777 1104 50-51 0.20009456990247543 1104 52-53 0.17281776902701296 1104 54-55 0.11321594280258296 1104 56-57 0.2805335074887054 1104 58-59 0.27963161709024575 1104 60-61 0.2894972596428431 1104 62-63 0.30369897721782024 1104 64-65 0.2302243234816217 1104 66-67 0.13410841026573195 1104 68-69 0.41056260943933154 1104 70-71 0.24280260741754134 1104 72-73 0.266293579221184 1104 74-75 0.2591279325590783 1104 76-77 0.2906029665774099 1104 78-79 0.3981988670947487 1104 80-81 0.4254807176301654 1104 82-83 0.5290270997916942 1104 84-85 0.6099958450199701 1104 86-87 0.7036775717332056 1104 88-89 0.9213117914706928 1104 90-91 0.8342282734097068 1104 92-93 0.8870256115988902 1104 94-95 1.032456083660577 1104 96-97 1.3275475418043143 1104 98-99 1.5873641127812448 1104 100-101 1.8392082671668923 1105 1 0.11383736986103798 1105 2 0.049094071469333755 1105 3 0.17170610987722057 1105 4 0.0668740278280282 1105 5 0.0620727897747102 1105 6 0.060955786349985885 1105 7 0.08644237172752867 1105 8 0.0402688203514856 1105 9 0.09429053772770146 1105 10-11 0.06394503593395484 1105 12-13 0.05988569151005407 1105 14-15 8.029525691455319E-4 1105 16-17 0.09867617810626683 1105 18-19 0.0669465187321876 1105 20-21 0.09082832093201887 1105 22-23 0.11996075128687522 1105 24-25 0.06706540515529014 1105 26-27 0.0762906561358605 1105 28-29 0.1428296706472949 1105 30-31 0.012690632763373344 1105 32-33 0.04591764624975525 1105 34-35 0.13773589595167834 1105 36-37 0.1543280526444022 1105 38-39 0.13678850183443814 1105 40-41 0.14472154926655634 1105 42-43 0.06084745311904527 1105 44-45 0.1414761098526185 1105 46-47 0.011103716026582333 1105 48-49 0.12618419077927 1105 50-51 0.1905145389785332 1105 52-53 0.17624344807543224 1105 54-55 0.29677055278346387 1105 56-57 0.2225024130976223 1105 58-59 0.3209467369509582 1105 60-61 0.3035317386230858 1105 62-63 0.32589055957413393 1105 64-65 0.39285809388960047 1105 66-67 0.5556784700439081 1105 68-69 0.5240848345023323 1105 70-71 0.5856353137496377 1105 72-73 0.45858207283551167 1105 74-75 0.4682724106870815 1105 76-77 0.32610158473975304 1105 78-79 0.5823409102665238 1105 80-81 0.7542850323726071 1105 82-83 0.8819679181804823 1105 84-85 0.9838803306963406 1105 86-87 0.993216695392455 1105 88-89 1.305108070857571 1105 90-91 1.3867578885648726 1105 92-93 1.3996599907459277 1105 94-95 1.465181286675051 1105 96-97 1.608851947857616 1105 98-99 2.190591038527252 1105 100-101 2.3247248431314382 1106 1 0.06989336026546056 1106 2 0.05085690907495888 1106 3 0.1408618315132273 1106 4 0.07417003921497667 1106 5 -0.05025965099051177 1106 6 0.021031451924486078 1106 7 -0.0013712667880056983 1106 8 -0.010043235430586606 1106 9 -0.0015262743633215337 1106 10-11 0.04907443647872611 1106 12-13 -0.040529390084152794 1106 14-15 -0.04903997477979516 1106 16-17 0.024143622448626445 1106 18-19 -0.006316965377166639 1106 20-21 -0.0556000188226875 1106 22-23 -0.08383348962168924 1106 24-25 0.0317866663022599 1106 26-27 -0.029010861604156446 1106 28-29 -0.018114050157905126 1106 30-31 0.09194441214373938 1106 32-33 0.08292320187808144 1106 34-35 -0.13098573569525485 1106 36-37 0.0069036823540145065 1106 38-39 0.08809629187697254 1106 40-41 0.12006108067765098 1106 42-43 -0.023000151636075827 1106 44-45 0.10694796089916281 1106 46-47 -0.22372644653808038 1106 48-49 -0.0367844152738499 1106 50-51 0.019788355920361766 1106 52-53 -0.018273167974953708 1106 54-55 0.14621385163037104 1106 56-57 0.20775383225596045 1106 58-59 0.3409605963942539 1106 60-61 0.3289760111239346 1106 62-63 0.2566586796111352 1106 64-65 0.26739192820874536 1106 66-67 0.21024089024451342 1106 68-69 -0.02617381377157102 1106 70-71 0.042785339416436585 1106 72-73 -0.020274791300153083 1106 74-75 0.36484054556903445 1106 76-77 0.44646152483601753 1106 78-79 0.5380589130097171 1106 80-81 0.6707641100397552 1106 82-83 0.6320248342110943 1106 84-85 0.5743610699997141 1106 86-87 0.712933078913565 1106 88-89 0.8639903554077861 1106 90-91 0.7531733918055004 1106 92-93 0.7570669338155476 1106 94-95 1.0305662667061029 1106 96-97 1.1733841230354898 1106 98-99 1.4473816667492443 1106 100-101 1.8476873427961813 1107 1 0.08346102330628469 1107 2 0.07818439892817963 1107 3 0.13029222356485803 1107 4 0.09473741776334066 1107 5 0.04854540561898091 1107 6 0.04909952293800757 1107 7 0.04582715806958504 1107 8 0.08630392643811291 1107 9 0.09082508208020812 1107 10-11 0.06658250773244845 1107 12-13 0.04600068016822689 1107 14-15 0.12447805596189454 1107 16-17 0.0765818128964284 1107 18-19 0.1388767259462682 1107 20-21 0.10359047627675722 1107 22-23 0.015331648867594083 1107 24-25 0.13890017879327132 1107 26-27 0.09485910657622298 1107 28-29 0.07653478931553792 1107 30-31 0.10437252900633354 1107 32-33 0.05459758637226031 1107 34-35 0.13121580056401427 1107 36-37 0.18380026746867628 1107 38-39 0.19883104056653877 1107 40-41 0.18863622384341028 1107 42-43 0.16735270158307713 1107 44-45 0.28927287230490606 1107 46-47 0.34399814674655715 1107 48-49 0.3919960879099875 1107 50-51 0.3239232548880011 1107 52-53 0.35872798125448213 1107 54-55 0.3558803649163167 1107 56-57 0.36286767803102293 1107 58-59 0.4856771811035685 1107 60-61 0.5380682850143685 1107 62-63 0.49224635921106596 1107 64-65 0.6087518021613292 1107 66-67 0.6021664838316454 1107 68-69 0.8221959843889195 1107 70-71 0.8023062661262266 1107 72-73 0.7048753929443663 1107 74-75 0.7064556370307322 1107 76-77 0.7750870959790248 1107 78-79 1.051276477679206 1107 80-81 1.0903333629355352 1107 82-83 1.2282162383352606 1107 84-85 1.228784927732466 1107 86-87 1.4682101137665207 1107 88-89 1.542739894702649 1107 90-91 1.7356291397166608 1107 92-93 1.8745535367729182 1107 94-95 1.9601937917643326 1107 96-97 2.06948770231093 1107 98-99 2.311015048298671 1107 100-101 2.5489237214999285 1108 1 0.07089835679240508 1108 2 0.07013658616856588 1108 3 0.142613534476709 1108 4 0.03447085231801594 1108 5 0.03392584507238183 1108 6 0.04175930464368349 1108 7 0.041096731507572315 1108 8 0.03005374739093014 1108 9 0.07432695849468018 1108 10-11 0.0342644912887593 1108 12-13 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1777.0 11 2965.0 12 4604.0 13 6675.0 14 9422.0 15 12431.0 16 16379.0 17 22279.0 18 22704.0 19 17807.0 20 15838.0 21 16141.0 22 16971.0 23 18745.0 24 21666.0 25 25384.0 26 29404.0 27 35128.0 28 43010.0 29 52331.0 30 62890.0 31 78251.0 32 98466.0 33 127676.0 34 175564.0 35 260655.0 36 399112.0 37 544047.0 38 340688.0 39 23060.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.1520386121695 18.77539105164729 14.807787767248742 23.26478256893447 2 15.963249475497454 19.687307417801975 39.655427887439934 24.69401521926064 3 19.892505110959437 23.24027839403051 29.42275212635482 27.44446436865523 4 13.300967500651936 16.50138314215874 36.10314522573351 34.094504131455814 5 14.005547763802321 36.92214121638675 34.110600653655325 14.961710366155605 6 33.20072298067392 35.546496554819775 16.753311997469726 14.49946846703657 7 29.313828106998134 32.2832208702514 19.810062054701906 18.592888968048555 8 26.829206929339634 34.80496007111603 19.089708503372698 19.276124496171644 9 26.140568136573854 16.814671729868163 19.92287789412563 37.12188223943235 10-11 25.42178148390567 26.33614887058366 26.549979812953335 21.692089832557336 12-13 26.127429583947166 23.496104958266127 27.232365885927813 23.1440995718589 14-15 22.792473566150615 24.977486750628273 25.57291477392305 26.657124909298062 16-17 22.402199610236096 28.23100699658158 26.54048592696719 22.826307466215138 18-19 22.41872367547216 26.543031155941044 28.901441287242708 22.136803881344093 20-21 22.818451160325885 25.718816603775995 29.325409202018626 22.137323033879493 22-23 22.612068060098693 25.638727341487755 29.250830943567717 22.498373654845828 24-25 22.492363829361114 25.871967121885003 29.26754462245259 22.368124426301293 26-27 22.104993225625034 26.246169442456157 29.04651071757247 22.60232661434634 28-29 22.251993700666652 26.50815700571209 28.97242074866383 22.26742854495743 30-31 22.37129898857025 26.156023831516496 28.867020569239227 22.605656610674025 32-33 22.110427536796198 26.085695059295244 28.89794109013796 22.905936313770596 34-35 22.741023345950715 26.238402613104107 28.771424885439046 22.249149155506135 36-37 22.815615019307902 25.842157245286046 28.71455974427063 22.627667991135418 38-39 22.32117660944896 26.23023993970202 28.69274692723361 22.75583652361541 40-41 22.83684965885937 25.980112745569926 28.73248511192073 22.450552483649975 42-43 22.44502329319226 25.980252261867125 28.642528234520416 22.932196210420198 44-45 22.55449629369289 26.334989456195284 28.633199405712823 22.477314844399004 46-47 22.959194064910747 26.497839676392747 27.97408410386007 22.568882154836437 48-49 22.667172508810637 26.57296606531354 27.93838043988299 22.82148098599283 50-51 22.758389205461004 26.85240613222975 27.8585437132425 22.530660949066743 52-53 23.04182652238486 26.511822101601346 27.84690271600639 22.599448660007404 54-55 22.696490249317016 26.767744334148937 27.90071487304125 22.635050543492802 56-57 22.770948703336675 26.904700766468814 27.738220129460668 22.586130400733843 58-59 22.70447721140011 26.56874917384861 27.7135204492187 23.013253165532586 60-61 22.458119365947027 26.807938720832112 27.92523484663635 22.808707066584507 62-63 22.543280349158035 26.807659177159206 28.04444025572655 22.60462021795621 64-65 22.291211825815537 26.813130246186123 27.887117070094774 23.008540857903558 66-67 22.300148597904297 26.846102330083827 27.850645727979973 23.003103344031903 68-69 22.70746682906862 26.505855455607357 27.939698291784392 22.846979423539633 70-71 22.491524834859575 26.655647321312685 27.62598334478797 23.226844499039768 72-73 22.505242442237293 26.770859249361344 27.582913651754875 23.14098465664649 74-75 22.496736327618795 27.123224049062312 27.35788099506366 23.022158628255234 76-77 22.609232688559196 26.738631857356054 27.61881504631839 23.03332040776636 78-79 22.661447453177434 26.95402025733193 27.571731904838543 22.812800384652093 80-81 22.723536091463696 26.987085652968528 27.382540981691644 22.90683727387613 82-83 22.64428531550301 27.057797191668374 27.42787780910038 22.870039683728233 84-85 22.652887989071736 27.125992750299478 27.33064812594263 22.89047113468616 86-87 22.76005133266655 27.07119262589113 27.197287922786153 22.971468118656173 88-89 22.606486214497938 27.254320948212516 26.993286955688315 23.145905881601227 90-91 22.613416326823206 27.117820630146948 27.16087223836214 23.107890804667704 92-93 22.689688414886664 26.859704632503846 27.291801696999325 23.158805255610172 94-95 22.503953547809974 26.803037442893196 27.334969330053354 23.358039679243475 96-97 22.70385725344833 26.52189461615459 27.33656686332615 23.43768126707093 98-99 22.478022840351954 26.802829944925815 27.032036155437712 23.68711105928452 100-101 22.82834224801813 26.41376477652186 27.169271583690907 23.588621391769095 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 676.0 1 460.5 2 360.5 3 509.0 4 429.0 5 376.0 6 777.5 7 1806.0 8 2955.0 9 3538.5 10 3062.0 11 2318.0 12 2221.5 13 2387.5 14 2564.0 15 2651.0 16 2750.5 17 2997.0 18 3252.5 19 3555.5 20 4156.0 21 4883.5 22 5782.0 23 7153.5 24 8850.0 25 11171.0 26 14476.5 27 18253.5 28 21377.0 29 24370.0 30 27709.5 31 32501.5 32 39378.0 33 43003.5 34 44247.5 35 47413.0 36 51924.5 37 56389.5 38 59993.5 39 63281.5 40 67269.0 41 71951.5 42 74622.0 43 76695.5 44 80140.0 45 84633.5 46 87321.0 47 90181.5 48 91790.0 49 90841.0 50 91615.5 51 91045.5 52 90817.0 53 92509.5 54 94028.5 55 89483.0 56 81724.5 57 75653.5 58 71007.0 59 63599.5 60 51263.5 61 42102.5 62 34924.5 63 25731.5 64 17900.0 65 13264.5 66 9973.5 67 7671.5 68 5749.0 69 4175.0 70 2998.0 71 1884.0 72 1191.5 73 888.0 74 670.5 75 454.0 76 271.0 77 160.5 78 106.0 79 61.5 80 32.0 81 22.0 82 14.0 83 6.5 84 4.0 85 3.0 86 2.5 87 1.5 88 1.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.011181746916333777 3 0.005910351941490711 4 0.0 5 3.5941329373929997E-4 6 1.1980443124643333E-4 7 0.0 8 1.597392416619111E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.9934810415477775E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0017770990634887608 26-27 0.021125514709787744 28-29 2.595762677006055E-4 30-31 7.986962083095555E-5 32-33 0.003434393695731088 34-35 0.015654445682867286 36-37 0.00734800511644791 38-39 0.020546459958763315 40-41 0.022682972315991378 42-43 0.01807050171300369 44-45 0.009624289310130145 46-47 0.002316219004097711 48-49 2.3960886249286666E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 3.194784833238222E-4 68-69 2.3960886249286666E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.1964145728512775E-4 82-83 0.0012779139332952888 84-85 0.004612470602987683 86-87 8.9853323434825E-4 88-89 3.9934810415477775E-5 90-91 0.004372861740494816 92-93 5.590873458166889E-4 94-95 3.9934810415477775E-5 96-97 2.595762677006055E-4 98-99 1.597392416619111E-4 100-101 0.002655664892629272 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2504081.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.44935030149933 #Duplication Level Percentage of deduplicated Percentage of total 1 75.18411780034779 40.1854224941998 2 13.867048984065658 14.823695175947513 3 4.683841055162594 7.510447839417893 4 2.074583672546541 4.4354059777484425 5 1.112145461280413 2.9721726173099676 6 0.6840652320463704 2.1937705330033728 7 0.4507063339737341 1.6862972509366667 8 0.3231927553938618 1.3819554238362646 9 0.23192539188215186 1.115663536306948 >10 1.2472454047785362 12.25239554416626 >50 0.09282724379824532 3.4255190254956345 >100 0.042975142667136 4.191656545733753 >500 0.0027002647049445055 0.9476697061777558 >1k 0.002400235293281602 1.941884996958057 >5k 2.250220587451502E-4 0.936043332761664 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9609 0.3837335932823259 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8493 0.3391663448586527 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5246 0.2094980154395964 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2788 0.11133825143835203 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2609 0.10418992037398152 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 3.9934810415477775E-5 7 0.0 0.0 0.0 3.9934810415477775E-5 3.9934810415477775E-5 8 0.0 0.0 0.0 7.986962083095555E-5 3.9934810415477775E-5 9 0.0 0.0 0.0 7.986962083095555E-5 3.9934810415477775E-5 10-11 0.0 0.0 0.0 9.983702603869443E-5 3.9934810415477775E-5 12-13 0.0 0.0 0.0 1.1980443124643332E-4 7.986962083095555E-5 14-15 0.0 0.0 0.0 1.1980443124643332E-4 9.983702603869443E-5 16-17 0.0 0.0 0.0 1.3977183645417222E-4 1.3977183645417222E-4 18-19 0.0 0.0 0.0 1.597392416619111E-4 1.597392416619111E-4 20-21 0.0 0.0 0.0 1.7970664686964998E-4 1.597392416619111E-4 22-23 0.0 0.0 0.0 2.995110781160833E-4 1.597392416619111E-4 24-25 0.0 0.0 0.0 4.991851301934722E-4 1.597392416619111E-4 26-27 0.0 0.0 0.0 8.186636135172943E-4 1.597392416619111E-4 28-29 0.0 0.0 0.0 0.0030949478071995275 1.597392416619111E-4 30-31 0.0 0.0 0.0 0.009644256715337883 1.597392416619111E-4 32-33 0.0 0.0 0.0 0.023980853654494403 1.9967405207738887E-4 34-35 0.0 0.0 0.0 0.04684353261735543 1.9967405207738887E-4 36-37 0.0 0.0 0.0 0.0876968436723892 1.9967405207738887E-4 38-39 0.0 0.0 0.0 0.14616140612064865 1.9967405207738887E-4 40-41 0.0 0.0 0.0 0.20901879771461068 1.9967405207738887E-4 42-43 0.0 0.0 0.0 0.2714568737992102 1.9967405207738887E-4 44-45 0.0 0.0 0.0 0.35120669019891926 1.9967405207738887E-4 46-47 0.0 0.0 0.0 0.4409402092024978 1.9967405207738887E-4 48-49 0.0 0.0 0.0 0.5363444712850742 1.9967405207738887E-4 50-51 0.0 0.0 0.0 0.638837162216398 1.9967405207738887E-4 52-53 0.0 0.0 0.0 0.7342813591093899 1.9967405207738887E-4 54-55 0.0 0.0 0.0 0.8384912468885791 1.9967405207738887E-4 56-57 0.0 0.0 0.0 0.9593739180162303 1.9967405207738887E-4 58-59 0.0 0.0 0.0 1.0883034534426002 1.9967405207738887E-4 60-61 0.0 0.0 0.0 1.2262582560228683 2.3960886249286664E-4 62-63 0.0 0.0 0.0 1.3591413376803705 2.3960886249286664E-4 64-65 0.0 0.0 0.0 1.5003308599042922 2.3960886249286664E-4 66-67 0.0 0.0 0.0 1.6639437781765047 2.3960886249286664E-4 68-69 0.0 0.0 0.0 1.8398366506514765 2.595762677006055E-4 70-71 0.0 0.0 0.0 2.023736452614752 2.7954367290834443E-4 72-73 0.0 0.0 0.0 2.2101122128237867 2.995110781160833E-4 74-75 0.0 0.0 0.0 2.3969871581630144 3.194784833238222E-4 76-77 0.0 0.0 0.0 2.5973600694226744 3.194784833238222E-4 78-79 0.0 0.0 0.0 2.820216278946248 3.194784833238222E-4 80-81 0.0 0.0 0.0 3.057169476546486 3.394458885315611E-4 82-83 0.0 0.0 0.0 3.3009515267277694 3.5941329373929997E-4 84-85 0.0 0.0 0.0 3.545053055392377 3.5941329373929997E-4 86-87 0.0 0.0 0.0 3.798699003746284 3.793806989470388E-4 88-89 0.0 0.0 0.0 4.073969651940173 4.392829145702555E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4610 0.0 58.01979 1 GTATCAA 8165 0.0 42.591557 1 TGGTATC 1850 0.0 33.384148 2 TCAACGC 10580 0.0 33.04061 4 ATCAACG 10505 0.0 33.007862 3 TATCAAC 10560 0.0 32.931828 2 CAACGCA 10770 0.0 32.50182 5 AACGCAG 11080 0.0 31.63534 6 GTGGTAT 2060 0.0 31.134022 1 ACGCAGA 12655 0.0 27.66058 7 CGCAGAG 12615 0.0 27.447083 8 TAAGGTG 1405 0.0 25.015621 5 GCAGAGT 14270 0.0 24.030851 9 TACATGG 8520 0.0 23.698343 2 GTACATG 8715 0.0 23.38614 1 GTAAGGT 1390 0.0 22.893696 4 GAGTACT 9275 0.0 22.480978 12-13 ACATGGG 8860 0.0 22.35594 3 CAGAGTA 13475 0.0 21.871233 10-11 GTATACG 110 0.002875419 21.59464 1 >>END_MODULE