FastQCFastQC Report
Thu 26 May 2016
SRR938101_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938101_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2519464
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102740.40778514795210413No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84840.3367382903665224No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52590.2087348737668012No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA27840.11049969358561978No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA25970.10307747997193055No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43150.058.0918851
GTATCAA80250.044.741421
ATCAACG103900.033.984233
TATCAAC104900.033.841472
TCAACGC104900.033.6602554
CAACGCA107700.032.5645265
AACGCAG112000.031.3991456
TGGTATC19000.028.5138192
GTGGTAT20750.028.3024441
ACGCAGA126250.027.4901377
CGCAGAG126400.027.307068
GCAGAGT139000.024.3856459
GAGTACT88600.022.98879212-13
CGCGTAA652.9517728E-421.91389376-77
CAGAGTA132550.021.59010310-11
GTACTTT94200.021.43404414-15
AGAGTAC128650.021.39472610-11
GTACATG87050.020.9522911
GTAAGGT12750.020.872864
TACATGG86000.020.7775122
Produced by FastQC (version 0.11.5)