##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938101_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2519464 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.781860744983852 33.0 31.0 34.0 30.0 34.0 2 31.97390397322605 34.0 31.0 34.0 30.0 34.0 3 32.04494051115634 34.0 31.0 34.0 30.0 34.0 4 35.5724281037554 37.0 35.0 37.0 33.0 37.0 5 35.58594645527779 37.0 35.0 37.0 33.0 37.0 6 35.60378596399869 37.0 35.0 37.0 33.0 37.0 7 35.54243362874008 37.0 35.0 37.0 33.0 37.0 8 35.52282906205447 37.0 35.0 37.0 33.0 37.0 9 37.168305639612235 39.0 37.0 39.0 34.0 39.0 10-11 37.085744428179964 39.0 37.0 39.0 33.0 39.0 12-13 36.9812392635894 39.0 37.0 39.0 33.0 39.0 14-15 38.21460874217691 40.0 38.0 41.0 33.0 41.0 16-17 38.13157362042085 40.0 38.0 41.0 33.0 41.0 18-19 38.065098171674606 40.0 38.0 41.0 33.0 41.0 20-21 37.948782756967354 40.0 38.0 41.0 33.0 41.0 22-23 37.79338740303493 40.0 38.0 41.0 32.5 41.0 24-25 37.741522601632724 40.0 38.0 41.0 32.0 41.0 26-27 37.75045466019756 40.0 38.0 41.0 32.0 41.0 28-29 37.65492819107556 40.0 38.0 41.0 32.0 41.0 30-31 37.46824066547488 40.0 37.0 41.0 32.0 41.0 32-33 37.24194511213496 40.0 37.0 41.0 31.0 41.0 34-35 37.029959150041435 40.0 36.0 41.0 31.0 41.0 36-37 36.8064102126484 40.0 36.0 41.0 30.0 41.0 38-39 36.55502539429021 39.0 36.0 41.0 30.0 41.0 40-41 36.32051559379296 39.0 35.0 41.0 30.0 41.0 42-43 36.02526906516624 39.0 35.0 40.5 29.0 41.0 44-45 35.799842744329744 39.0 35.0 40.5 28.0 41.0 46-47 35.648701866746265 39.0 35.0 41.0 27.0 41.0 48-49 35.38479216214242 39.0 35.0 41.0 26.0 41.0 50-51 34.73560348550326 38.5 34.0 39.5 25.0 40.5 52-53 34.80795835939708 38.5 34.5 40.0 25.5 41.0 54-55 34.93827476796652 39.0 35.0 40.0 25.5 41.0 56-57 34.71674887992049 38.0 34.0 40.0 24.5 41.0 58-59 34.43573553740002 38.0 34.0 40.0 23.5 41.0 60-61 34.046063964398776 38.0 34.0 40.0 22.5 41.0 62-63 33.609644154470956 37.0 33.5 40.0 21.5 41.0 64-65 33.16323610895016 36.5 33.0 39.5 20.0 41.0 66-67 32.721293497347055 36.0 32.5 39.0 18.5 41.0 68-69 32.21948874840045 35.0 32.0 39.0 15.0 40.0 70-71 31.723973630899273 35.0 31.0 38.0 10.0 40.0 72-73 31.22566962655549 35.0 31.0 37.0 7.5 39.5 74-75 30.69178325231081 35.0 30.5 37.0 4.5 39.0 76-77 30.13807837698812 34.5 30.0 36.0 2.0 39.0 78-79 29.559399935859375 34.0 29.0 36.0 2.0 38.0 80-81 29.016907961375914 34.0 29.0 35.0 2.0 37.0 82-83 28.46235667586439 34.0 27.5 35.0 2.0 37.0 84-85 27.95363359031921 34.0 26.5 35.0 2.0 36.0 86-87 27.436953256724447 33.0 26.0 35.0 2.0 36.0 88-89 26.85304929937479 33.0 25.0 35.0 2.0 35.0 90-91 26.216178123600894 32.5 24.5 35.0 2.0 35.0 92-93 25.57677347245287 32.0 21.5 34.5 2.0 35.0 94-95 24.933020872693557 32.0 18.5 34.0 2.0 35.0 96-97 24.048590295396163 31.0 5.0 34.0 2.0 35.0 98-99 22.91692598108169 31.0 2.0 34.0 2.0 35.0 100-101 20.030625363172483 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.38086197630713414 1101 2 0.2097705179176934 1101 3 0.2634364358393597 1101 4 0.1610069440983466 1101 5 0.3323641250108835 1101 6 0.1998743832819656 1101 7 0.19831529124684266 1101 8 0.12699625234346001 1101 9 0.09659016634544315 1101 10-11 0.19819237061147277 1101 12-13 0.12362202493186913 1101 14-15 0.2256375143710727 1101 16-17 0.3151499789829586 1101 18-19 0.210480565085021 1101 20-21 0.3134607690646405 1101 22-23 0.3227240541511165 1101 24-25 0.41907557322611666 1101 26-27 0.24377923327268292 1101 28-29 0.2906656971353456 1101 30-31 0.4390992117550141 1101 32-33 0.35428545102277553 1101 34-35 0.35699717975334266 1101 36-37 0.41451837775839806 1101 38-39 0.3691713947491593 1101 40-41 0.4110110084810543 1101 42-43 0.39013407254984145 1101 44-45 0.511209195128572 1101 46-47 0.5241512743958978 1101 48-49 0.5671320171468892 1101 50-51 0.620018897121966 1101 52-53 0.5233141518057067 1101 54-55 0.5879643393652998 1101 56-57 0.5603689010601514 1101 58-59 0.4209363848172458 1101 60-61 0.43282847135665037 1101 62-63 0.556102518779241 1101 64-65 0.4636528305942349 1101 66-67 0.3988991811478826 1101 68-69 0.5100696707767156 1101 70-71 0.6392867174845271 1101 72-73 0.6208600823772876 1101 74-75 0.7100205355272031 1101 76-77 0.597784145692291 1101 78-79 0.6866406925944126 1101 80-81 0.7533523815796812 1101 82-83 0.8260017274485314 1101 84-85 0.9297410552325154 1101 86-87 0.9210568360407514 1101 88-89 0.8446824681434215 1101 90-91 0.9387346721285006 1101 92-93 1.073415035571628 1101 94-95 1.2804758739612083 1101 96-97 1.2061174352686912 1101 98-99 1.3302158716890915 1101 100-101 1.2689612582460796 1102 1 0.4178585406246178 1102 2 0.3319385295292001 1102 3 0.2840078655773013 1102 4 0.37086876147844805 1102 5 0.2787940160611697 1102 6 0.3355566918646389 1102 7 0.19680975598056705 1102 8 0.29889102206291085 1102 9 0.34514801666563244 1102 10-11 0.5127294108618301 1102 12-13 0.3952198724757494 1102 14-15 0.4337950658297487 1102 16-17 0.41284625485009485 1102 18-19 0.3565429013899575 1102 20-21 0.38750656591638943 1102 22-23 0.47932034002113255 1102 24-25 0.5823392878868674 1102 26-27 0.5296661925813808 1102 28-29 0.5787553310912372 1102 30-31 0.5523266914991893 1102 32-33 0.670844705828749 1102 34-35 0.6797699572260427 1102 36-37 0.6609549033708646 1102 38-39 0.5984859526117248 1102 40-41 0.6680567093868177 1102 42-43 0.8423610399016681 1102 44-45 0.7549671948795194 1102 46-47 0.720413974478987 1102 48-49 0.6756818243363085 1102 50-51 0.7423217434512139 1102 52-53 0.6203991293196509 1102 54-55 0.6508658069304616 1102 56-57 0.539798492024083 1102 58-59 0.5403608518577627 1102 60-61 0.7491142801302004 1102 62-63 0.66506591436859 1102 64-65 0.6908030922001203 1102 66-67 0.7489852263159804 1102 68-69 0.6925904573704145 1102 70-71 0.7359916876902481 1102 72-73 0.697871834738681 1102 74-75 0.6869620635995659 1102 76-77 0.6851397929295544 1102 78-79 0.7666443262930898 1102 80-81 0.8182106367102371 1102 82-83 0.8916432691983012 1102 84-85 0.9222149099539756 1102 86-87 0.9123469827972706 1102 88-89 0.9532442175447748 1102 90-91 1.0154338855756357 1102 92-93 1.006165888368045 1102 94-95 1.1097917587362183 1102 96-97 1.1286354455532859 1102 98-99 1.1742193502410814 1102 100-101 1.1510504920868385 1103 1 0.4428423957903398 1103 2 0.41998463514568485 1103 3 0.3741947810167545 1103 4 0.26338091688339205 1103 5 0.3200622275781697 1103 6 0.22210989209224863 1103 7 0.260557142340609 1103 8 0.33859512779262246 1103 9 0.2927612200214611 1103 10-11 0.3357073723234194 1103 12-13 0.29366344328128946 1103 14-15 0.5060763285141263 1103 16-17 0.4467442655570224 1103 18-19 0.4232239936539699 1103 20-21 0.5147072291740002 1103 22-23 0.49276675596388486 1103 24-25 0.692440628507839 1103 26-27 0.4619559570923002 1103 28-29 0.5926415198452233 1103 30-31 0.5718669787381003 1103 32-33 0.6344390401194175 1103 34-35 0.7229201814305384 1103 36-37 0.7485338883141992 1103 38-39 0.9688735917619624 1103 40-41 0.8793223972239304 1103 42-43 0.8481173330285259 1103 44-45 0.8812631716731829 1103 46-47 0.8997950299913171 1103 48-49 0.9936039817659506 1103 50-51 0.7976599981202597 1103 52-53 0.8418676807177974 1103 54-55 0.9332261481166242 1103 56-57 0.8974150634850062 1103 58-59 0.9262296838353805 1103 60-61 0.9364603764388164 1103 62-63 0.9283939668457748 1103 64-65 0.8407882667802369 1103 66-67 0.953149202176931 1103 68-69 1.0000166973080766 1103 70-71 1.1693043581945695 1103 72-73 1.1171151337928258 1103 74-75 1.073930375583977 1103 76-77 1.1234351479191957 1103 78-79 1.1866067301797507 1103 80-81 1.150426322100433 1103 82-83 1.1462815145594796 1103 84-85 1.2338548316911115 1103 86-87 1.2018884886952925 1103 88-89 1.1625137033761845 1103 90-91 1.4982490648864513 1103 92-93 1.4291529657836008 1103 94-95 1.2120438791550896 1103 96-97 1.1631546845342555 1103 98-99 1.2720923582851924 1103 100-101 1.2530564817483878 1104 1 0.42585352465509274 1104 2 0.3809571101608178 1104 3 0.30257984262242843 1104 4 0.2729194349239208 1104 5 0.263663428682662 1104 6 0.27952035738933034 1104 7 0.27634804371934507 1104 8 0.28517647013500635 1104 9 0.32905493028839317 1104 10-11 0.3413392194392486 1104 12-13 0.2269850094619983 1104 14-15 0.36575410207859704 1104 16-17 0.43461931905689966 1104 18-19 0.5204450457905949 1104 20-21 0.4860112582601275 1104 22-23 0.44528816526505466 1104 24-25 0.4672681776800829 1104 26-27 0.4128766892894262 1104 28-29 0.48356589261650385 1104 30-31 0.49548732313569843 1104 32-33 0.5016873021638162 1104 34-35 0.6487620953751332 1104 36-37 0.5022612467125072 1104 38-39 0.6570330339490624 1104 40-41 0.5798596134564065 1104 42-43 0.6335682699687624 1104 44-45 0.7003999281317732 1104 46-47 0.6887444981451054 1104 48-49 0.6991100295896331 1104 50-51 0.7625811780866272 1104 52-53 0.702343388345497 1104 54-55 0.7415478701438261 1104 56-57 0.8144927047847901 1104 58-59 0.7450869852615583 1104 60-61 0.7620843546423828 1104 62-63 0.762409789094562 1104 64-65 0.7938312720421479 1104 66-67 0.8464180405897608 1104 68-69 0.7870037463751416 1104 70-71 0.7596751107764099 1104 72-73 0.928964953340401 1104 74-75 0.9963640602279646 1104 76-77 0.9473965556957431 1104 78-79 1.0185181794470815 1104 80-81 1.0221260944220631 1104 82-83 1.1633283605915103 1104 84-85 1.1164612761842 1104 86-87 1.1532713280189775 1104 88-89 1.2628889104961765 1104 90-91 1.2936036459452431 1104 92-93 1.4020342504716936 1104 94-95 1.389332716502821 1104 96-97 1.1561790843961113 1104 98-99 1.4405179827264831 1104 100-101 1.2209767716976785 1105 1 0.40111973036526294 1105 2 0.3463720376835191 1105 3 0.26284434808322743 1105 4 0.3268713478711476 1105 5 0.21437936702028537 1105 6 0.20991548200760946 1105 7 0.21560780181719252 1105 8 0.19710838681612586 1105 9 0.23808842186716106 1105 10-11 0.2851030548017732 1105 12-13 0.1897713847970408 1105 14-15 0.29628833043209113 1105 16-17 0.250142845840827 1105 18-19 0.2190837628024198 1105 20-21 0.3079076363955835 1105 22-23 0.24261960720986764 1105 24-25 0.24568884604477859 1105 26-27 0.243335906641299 1105 28-29 0.3184200878984811 1105 30-31 0.3042126028433998 1105 32-33 0.3202084352633392 1105 34-35 0.38532037392264584 1105 36-37 0.4554457971132351 1105 38-39 0.448013127696143 1105 40-41 0.4537538679470572 1105 42-43 0.591330075033504 1105 44-45 0.6727077832838617 1105 46-47 0.47762803439709245 1105 48-49 0.36224470600884473 1105 50-51 0.3245236796535309 1105 52-53 0.426026778576059 1105 54-55 0.47015014847797687 1105 56-57 0.5412712989786073 1105 58-59 0.5632697353982508 1105 60-61 0.574788717949005 1105 62-63 0.5200496596089081 1105 64-65 0.6033596695404455 1105 66-67 0.6941792955738322 1105 68-69 0.8438109114260186 1105 70-71 0.8584789353373488 1105 72-73 0.8522965419733914 1105 74-75 0.8113495914392068 1105 76-77 0.8644751438886757 1105 78-79 0.9164808806432596 1105 80-81 0.788191867402098 1105 82-83 0.9193603286134895 1105 84-85 0.9697335894568155 1105 86-87 1.024093965339116 1105 88-89 1.0694257859045742 1105 90-91 0.9754444826012865 1105 92-93 1.081842122292894 1105 94-95 1.2414629456030895 1105 96-97 1.3820920516313073 1105 98-99 1.1756988925932639 1105 100-101 1.0798420719417123 1106 1 0.022759728067498486 1106 2 -0.006188055248298241 1106 3 0.06438672269548107 1106 4 0.0194891402298083 1106 5 -0.11245964264308839 1106 6 0.1316357419103369 1106 7 0.19457112693815048 1106 8 0.10620992887159986 1106 9 0.06552421210676584 1106 10-11 -0.5096750353727302 1106 12-13 -0.24479385895750738 1106 14-15 -0.1668460281724009 1106 16-17 -0.0025100353009079868 1106 18-19 0.1029305561440026 1106 20-21 -0.04557076461542664 1106 22-23 -0.10058523213807291 1106 24-25 -0.06976665391054127 1106 26-27 -0.0777413780389864 1106 28-29 -0.15011593275587387 1106 30-31 0.028627630225095402 1106 32-33 0.06467393368681229 1106 34-35 -0.2474488634747516 1106 36-37 -0.3817815826689852 1106 38-39 -0.09368148978984436 1106 40-41 0.09323864206402277 1106 42-43 -0.13360577903289794 1106 44-45 0.02081444105732544 1106 46-47 0.13183820966548154 1106 48-49 0.05922402293443696 1106 50-51 0.070431344613894 1106 52-53 0.08298238384560364 1106 54-55 -0.2712591632138057 1106 56-57 -0.0758789620730056 1106 58-59 0.021910846878135715 1106 60-61 -0.2761698010139355 1106 62-63 -0.17520649329457427 1106 64-65 -0.14874246610426667 1106 66-67 -0.40416176612115606 1106 68-69 -0.84289348751701 1106 70-71 -0.452365237914119 1106 72-73 -0.12331220612775695 1106 74-75 -0.2574474010419934 1106 76-77 -0.0826473046350138 1106 78-79 -0.021201504436586305 1106 80-81 0.03909078809091682 1106 82-83 0.12903383857685213 1106 84-85 0.08110513163994426 1106 86-87 0.032394082142158 1106 88-89 -0.21384864061163356 1106 90-91 -0.27590306070520043 1106 92-93 -0.29185054528436893 1106 94-95 -0.2795024826109085 1106 96-97 -0.25399737609760464 1106 98-99 -0.22653129556814378 1106 100-101 -0.3052677739803933 1107 1 0.24803901127410555 1107 2 0.1295110997599629 1107 3 0.11482382022398951 1107 4 0.11467292011295172 1107 5 0.13938090722581364 1107 6 0.0854077316934081 1107 7 0.1322728255979726 1107 8 0.2903527373422534 1107 9 0.22207305677684275 1107 10-11 0.2505323716898147 1107 12-13 0.140182619672224 1107 14-15 0.14335778137962762 1107 16-17 0.29115898103390947 1107 18-19 0.27557773135758623 1107 20-21 0.3133152979939524 1107 22-23 0.45284626385493 1107 24-25 0.43824256841349296 1107 26-27 0.3512305381009284 1107 28-29 0.3516621897245429 1107 30-31 0.43435380888592334 1107 32-33 0.48849082886593465 1107 34-35 0.4839900984106862 1107 36-37 0.4569171554512934 1107 38-39 0.439979569698167 1107 40-41 0.314447976968971 1107 42-43 0.35647091142671883 1107 44-45 0.35678806495664617 1107 46-47 0.40160441380515266 1107 48-49 0.3702970545142179 1107 50-51 0.3728259111032983 1107 52-53 0.3857816702688126 1107 54-55 0.3422491713715985 1107 56-57 0.3398641672109406 1107 58-59 0.2479614786644717 1107 60-61 0.3783998838229792 1107 62-63 0.4622834812900649 1107 64-65 0.3899186039646523 1107 66-67 0.5336817167769112 1107 68-69 0.5456323584799136 1107 70-71 0.5845330519986724 1107 72-73 0.5617130505122958 1107 74-75 0.6205932889182009 1107 76-77 0.6901117108321664 1107 78-79 0.7266394602041153 1107 80-81 0.774485424414344 1107 82-83 0.6282302182115558 1107 84-85 0.573545598131254 1107 86-87 0.8709314613345818 1107 88-89 0.9609163043993583 1107 90-91 0.9879648133142176 1107 92-93 0.8674519820725806 1107 94-95 0.9635246955229988 1107 96-97 0.9217526519418122 1107 98-99 0.7409255184342172 1107 100-101 0.5204714615274568 1108 1 0.17896202494605262 1108 2 0.18329229633035382 1108 3 0.10637908707072086 1108 4 0.11181596687659834 1108 5 0.16156740157123295 1108 6 0.19305976375311218 1108 7 0.1525335197077453 1108 8 0.18651912211559107 1108 9 0.13374999121774778 1108 10-11 0.3323023869542894 1108 12-13 0.09434516950872762 1108 14-15 0.18330829609951138 1108 16-17 0.2867768733068843 1108 18-19 0.37602223409285784 1108 20-21 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-0.8917019025213726 2316 76-77 -0.950433572761245 2316 78-79 -1.1148292315319566 2316 80-81 -1.079736556812982 2316 82-83 -1.1355033743990397 2316 84-85 -1.0492821089210551 2316 86-87 -1.0220338621120248 2316 88-89 -1.0956995396496403 2316 90-91 -0.9251140863956984 2316 92-93 -0.9481455220275947 2316 94-95 -1.0618870584983817 2316 96-97 -1.0662994029551633 2316 98-99 -1.0082237280412372 2316 100-101 -0.7874425495951058 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7612.0 3 2291.0 4 2433.0 5 2618.0 6 3024.0 7 3673.0 8 4489.0 9 5553.0 10 6881.0 11 8408.0 12 10371.0 13 12329.0 14 14322.0 15 17219.0 16 20662.0 17 26492.0 18 24228.0 19 19909.0 20 19682.0 21 20569.0 22 22083.0 23 24551.0 24 27023.0 25 30455.0 26 33644.0 27 39582.0 28 45671.0 29 53750.0 30 64286.0 31 78600.0 32 97316.0 33 125741.0 34 171694.0 35 247684.0 36 366503.0 37 483148.0 38 337967.0 39 36995.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.81521141681937 18.37012442571672 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26.492621335240663 28.822840957695444 22.209921910339265 20-21 22.93687941741202 25.691553125722415 29.289412828279698 22.082154628585865 22-23 22.657067753517925 25.601638220159266 29.16058096287931 22.5807130634435 24-25 22.493987867081756 25.864000160495692 29.23214530104165 22.409866671380897 26-27 22.09663569501141 26.225739598202548 28.98776941328612 22.689855293499924 28-29 22.21786875098425 26.526315873089317 28.92931946374325 22.32649591218318 30-31 22.340805821232244 26.1642067300054 28.808009755012552 22.686977693749803 32-33 22.1133742175336 26.114319532267803 28.800800737186616 22.97150551301198 34-35 22.703447180144085 26.282814741995224 28.655237300842558 22.358500777018136 36-37 22.807054474640946 25.834579651391053 28.666533346546192 22.69183252742181 38-39 22.39586476793718 26.21612210020743 28.624740714151848 22.763272417703543 40-41 22.87109067105787 26.03931436522159 28.64794924647599 22.441645717244548 42-43 22.461199786849978 26.03729966558318 28.589858537952857 22.911642009613985 44-45 22.5533153177402 26.34689187349068 28.590206393272698 22.509586415496425 46-47 22.97003098558949 26.52306112770553 27.94074762833888 22.5661602583661 48-49 22.681368706261672 26.591731154839703 27.878980134340793 22.847920004557828 50-51 22.736481577499323 26.8828444407743 27.822450335910993 22.558223645815385 52-53 23.042751214949487 26.560119801701504 27.80853722106582 22.58859176228319 54-55 22.672583703098486 26.81478711234211 27.88256524809053 22.63006393646888 56-57 22.799541215017943 26.87901948009927 27.749166576346845 22.572272728535943 58-59 22.716632329813237 26.543158536616833 27.681703621378478 23.058505512191452 60-61 22.433094603141328 26.7628138321377 28.004504627903682 22.799586936817292 62-63 22.516742428317553 26.75382382791092 28.11332548225478 22.61610826151674 64-65 22.294160476143592 26.774501926234585 27.98796619482124 22.943371402800583 66-67 22.261592950425435 26.83563484042152 27.893265541226658 23.009506667926388 68-69 22.684398570861454 26.448967844382693 28.01824136562128 22.848392219134578 70-71 22.4162607753208 26.61457333412483 27.756237669642005 23.212928220912367 72-73 22.483162250883208 26.68328355216203 27.684505498759687 23.149048698195074 74-75 22.43680921917883 27.05249313871946 27.498761743584144 23.011935898517567 76-77 22.585115870808124 26.713577271389212 27.623179044699793 23.078127813102878 78-79 22.722773466163815 26.8437682418784 27.491281114032567 22.942177177925217 80-81 22.736450101447335 26.8913844594034 27.342726855180207 23.029438583969057 82-83 22.697974491479098 26.95987531179 27.417193776864156 22.924956419866742 84-85 22.702388445363848 27.00433322674441 27.35079684647642 22.94248148141532 86-87 22.799584872084594 26.97047324304493 27.172043290323387 23.057898594547087 88-89 22.710299596691417 27.0805215494324 26.984965567683535 23.224213286192647 90-91 22.72012278904271 27.025661797993756 27.066644428030212 23.187570984933323 92-93 22.823704905424997 26.647600590722774 27.252537560904145 23.276156942948084 94-95 22.657736724222833 26.54019665464712 27.364243959779515 23.437822661350534 96-97 22.943007251110142 26.23552984406996 27.300086206451546 23.52137669836835 98-99 22.679135691468463 26.48323115744787 26.972746325410263 23.864886825673402 100-101 23.175593769667582 25.827961751608097 27.237507469457224 23.758937009267097 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 658.0 1 460.5 2 392.5 3 549.5 4 466.5 5 426.5 6 820.5 7 1820.0 8 3046.0 9 3570.0 10 2995.5 11 2372.0 12 2335.5 13 2458.0 14 2638.5 15 2754.5 16 2885.0 17 3106.5 18 3342.5 19 3688.5 20 4248.5 21 5055.5 22 6107.0 23 7514.5 24 9182.5 25 11327.5 26 14641.0 27 18458.0 28 21350.5 29 24488.5 30 27998.0 31 32550.0 32 39131.5 33 42738.5 34 44201.5 35 47196.5 36 51757.0 37 56601.0 38 60310.0 39 63637.0 40 67280.0 41 72076.5 42 74828.5 43 76740.5 44 80254.5 45 84355.5 46 86761.0 47 89754.5 48 91949.0 49 90972.0 50 91250.5 51 91098.0 52 91062.0 53 92611.5 54 94101.5 55 89750.0 56 82476.0 57 76471.5 58 71491.5 59 64222.5 60 52525.0 61 43158.5 62 35544.5 63 26527.0 64 18945.0 65 14409.5 66 10842.5 67 8174.5 68 6160.5 69 4545.5 70 3336.0 71 2175.5 72 1431.5 73 1048.0 74 737.5 75 493.0 76 335.0 77 211.0 78 129.5 79 75.5 80 40.0 81 31.0 82 27.0 83 24.0 84 17.5 85 12.0 86 8.5 87 8.0 88 8.5 89 5.0 90 3.5 91 3.0 92 2.0 93 3.5 94 2.5 95 0.5 96 1.0 97 0.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4932001409823677 2 0.04854207085316559 3 0.057075631959813675 4 0.0643390816459374 5 0.0789056720000762 6 0.10795947074457106 7 0.0701339650020798 8 0.052947769843109484 9 0.05215395020528176 10-11 0.07457935497391509 12-13 0.10807854369024522 14-15 0.055051391883352965 16-17 0.059774618728427945 18-19 0.05798852454331556 20-21 0.0742221361368926 22-23 0.06388263535418645 24-25 0.08980084652926178 26-27 0.06660146761374643 28-29 0.06658162212280072 30-31 0.07080871169423338 32-33 0.07080871169423338 34-35 0.0724955784246173 36-37 0.06805018845278202 38-39 0.04000850974651751 40-41 0.0435410071348509 42-43 0.04167553098595574 44-45 0.021234675311891736 46-47 0.028279824597612826 48-49 0.028597352452743918 50-51 0.025481610374270085 52-53 0.02520377350103038 54-55 0.02522361899197607 56-57 0.025283155464813155 58-59 0.021730812585534064 60-61 0.02861719794368961 62-63 0.019428735635833656 64-65 0.022226949859176396 66-67 0.015479482937640706 68-69 0.018416615597603297 70-71 0.04110001174853064 72-73 0.037369059450740316 74-75 0.03181232198594622 76-77 0.0222071043682307 78-79 0.014427671917518965 80-81 0.016789285340056456 82-83 0.021472821203240053 84-85 0.01748387752315572 86-87 0.009545681144878434 88-89 0.041477076076498816 90-91 0.05255086002419562 92-93 0.0339159440261897 94-95 0.053344679662023345 96-97 0.04330286124350259 98-99 0.033717489116732766 100-101 0.1675356345635421 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2519464.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.25307378791251 #Duplication Level Percentage of deduplicated Percentage of total 1 76.64528332137353 42.34887494851312 2 13.198811457446949 14.585498067421229 3 4.401047151094869 7.295141489505809 4 1.9282417198224107 4.261651281051159 5 1.0370926827744404 2.865127926312015 6 0.6102480156725197 2.0230847183328553 7 0.3891478901372624 1.5051131961715247 8 0.28285672642167503 1.2502962861107354 9 0.21087980757250835 1.0486581811366136 >10 1.1671964599278486 11.852551094503108 >50 0.08510482478302707 3.242705860935059 >100 0.038893572118657106 3.8957226785293733 >500 0.002959044514249101 1.1181950939876049 >1k 0.002020810887776259 1.749649725178571 >5k 1.4434363484116137E-4 0.5480316385524912 >10k+ 7.217181742058068E-5 0.4096978137588804 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10274 0.40778514795210413 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8484 0.3367382903665224 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5259 0.2087348737668012 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2784 0.11049969358561978 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2597 0.10307747997193055 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.969098189138642E-5 0.0 10-11 0.0 0.0 0.0 3.969098189138642E-5 0.0 12-13 0.0 0.0 0.0 3.969098189138642E-5 1.1907294567415928E-4 14-15 0.0 0.0 0.0 3.969098189138642E-5 1.7860941851123889E-4 16-17 0.0 0.0 0.0 3.969098189138642E-5 1.984549094569321E-4 18-19 0.0 0.0 0.0 3.969098189138642E-5 1.984549094569321E-4 20-21 0.0 0.0 0.0 3.969098189138642E-5 2.579913822940117E-4 22-23 0.0 0.0 0.0 1.984549094569321E-4 2.7783687323970494E-4 24-25 0.0 0.0 0.0 5.75519237425103E-4 2.7783687323970494E-4 26-27 0.0 3.969098189138642E-5 0.0 0.001210574947687286 3.175278551310914E-4 28-29 0.0 3.969098189138642E-5 0.0 0.003353887969822153 3.175278551310914E-4 30-31 0.0 3.969098189138642E-5 0.0 0.00958537212676982 3.175278551310914E-4 32-33 0.0 3.969098189138642E-5 0.0 0.023993198553343094 3.175278551310914E-4 34-35 1.984549094569321E-5 3.969098189138642E-5 0.0 0.04842299790749144 3.175278551310914E-4 36-37 3.969098189138642E-5 3.969098189138642E-5 0.0 0.08791552488942092 3.175278551310914E-4 38-39 3.969098189138642E-5 3.969098189138642E-5 0.0 0.1461223498331391 3.175278551310914E-4 40-41 3.969098189138642E-5 3.969098189138642E-5 0.0 0.20897301965814952 3.5721883702247777E-4 42-43 3.969098189138642E-5 3.969098189138642E-5 0.0 0.272141217338291 3.5721883702247777E-4 44-45 3.969098189138642E-5 3.969098189138642E-5 0.0 0.34705794565828285 3.77064327968171E-4 46-47 3.969098189138642E-5 3.969098189138642E-5 0.0 0.4367000282599791 4.1675530985955744E-4 48-49 3.969098189138642E-5 3.969098189138642E-5 0.0 0.5320377667630893 4.3660080080525066E-4 50-51 3.969098189138642E-5 3.969098189138642E-5 0.0 0.6323170325116771 4.3660080080525066E-4 52-53 3.969098189138642E-5 3.969098189138642E-5 0.0 0.7262258956666974 4.3660080080525066E-4 54-55 3.969098189138642E-5 3.969098189138642E-5 0.0 0.8302559592040212 4.3660080080525066E-4 56-57 3.969098189138642E-5 3.969098189138642E-5 0.0 0.9511943810270757 4.3660080080525066E-4 58-59 3.969098189138642E-5 3.969098189138642E-5 0.0 1.0775506218783042 4.3660080080525066E-4 60-61 3.969098189138642E-5 3.969098189138642E-5 0.0 1.2164095220253197 4.3660080080525066E-4 62-63 3.969098189138642E-5 7.938196378277285E-5 0.0 1.3531846456230374 4.7629178269663705E-4 64-65 3.969098189138642E-5 7.938196378277285E-5 0.0 1.4896025503837325 5.556737464794099E-4 66-67 3.969098189138642E-5 7.938196378277285E-5 0.0 1.648703851295355 5.556737464794099E-4 68-69 3.969098189138642E-5 7.938196378277285E-5 0.0 1.8224908155067903 5.556737464794099E-4 70-71 3.969098189138642E-5 7.938196378277285E-5 0.0 2.0028267917303046 5.75519237425103E-4 72-73 3.969098189138642E-5 7.938196378277285E-5 0.0 2.189334715637929 6.350557102621828E-4 74-75 3.969098189138642E-5 7.938196378277285E-5 0.0 2.3724093696119493 6.350557102621828E-4 76-77 3.969098189138642E-5 7.938196378277285E-5 0.0 2.5753295145316626 6.350557102621828E-4 78-79 3.969098189138642E-5 7.938196378277285E-5 0.0 2.8019054846586418 6.350557102621828E-4 80-81 3.969098189138642E-5 7.938196378277285E-5 0.0 3.036637951564301 6.350557102621828E-4 82-83 3.969098189138642E-5 7.938196378277285E-5 0.0 3.280102434486065 6.350557102621828E-4 84-85 3.969098189138642E-5 7.938196378277285E-5 0.0 3.525233938647268 6.350557102621828E-4 86-87 3.969098189138642E-5 7.938196378277285E-5 0.0 3.7802484972994255 6.549012012078759E-4 88-89 3.969098189138642E-5 7.938196378277285E-5 0.0 4.049393045504917 6.747466921535692E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4315 0.0 58.091885 1 GTATCAA 8025 0.0 44.74142 1 ATCAACG 10390 0.0 33.98423 3 TATCAAC 10490 0.0 33.84147 2 TCAACGC 10490 0.0 33.660255 4 CAACGCA 10770 0.0 32.564526 5 AACGCAG 11200 0.0 31.399145 6 TGGTATC 1900 0.0 28.513819 2 GTGGTAT 2075 0.0 28.302444 1 ACGCAGA 12625 0.0 27.490137 7 CGCAGAG 12640 0.0 27.30706 8 GCAGAGT 13900 0.0 24.385645 9 GAGTACT 8860 0.0 22.988792 12-13 CGCGTAA 65 2.9517728E-4 21.913893 76-77 CAGAGTA 13255 0.0 21.590103 10-11 GTACTTT 9420 0.0 21.434044 14-15 AGAGTAC 12865 0.0 21.394726 10-11 GTACATG 8705 0.0 20.952291 1 GTAAGGT 1275 0.0 20.87286 4 TACATGG 8600 0.0 20.777512 2 >>END_MODULE