FastQCFastQC Report
Thu 26 May 2016
SRR938101_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938101_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2519464
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98610.3913927724309615No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83480.3313403168292939No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54610.21675245210886124No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA28460.11296053446288574No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA26290.10434759139245489No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA25590.10156922266005784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47150.054.30611
GTATCAA82750.043.056111
TGGTATC21100.036.2480772
GTGGTAT23750.033.003641
ATCAACG108150.032.7198223
TCAACGC109200.032.490914
TATCAAC109250.032.4383622
CAACGCA112750.031.4257935
AACGCAG117550.030.2233736
CGCAGAG133350.026.464268
ACGCAGA133300.026.4385557
GCAGAGT147850.023.5475989
TAAGGTG14400.023.0887015
GTAAGGT13500.021.8133244
GAGTACT96700.021.12016512-13
AGAGTAC135500.020.73341410-11
CAGAGTA140900.020.71410810-11
GTACTTT99400.020.23589114-15
GTACATG87850.020.1701241
GTATAGG5900.020.129341
Produced by FastQC (version 0.11.5)