FastQCFastQC Report
Thu 26 May 2016
SRR938100_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938100_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2515572
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97080.3859162051414151No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80130.3185359035638813No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47900.1904139495907889No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA28690.11404960780291719No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA26660.10597987256973762No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44350.059.9101261
GTATCAA82550.044.3000071
ATCAACG105550.034.5179673
TATCAAC105550.034.4715352
TCAACGC106500.034.210064
CAACGCA109600.033.285845
AACGCAG114100.032.0898176
TGGTATC18700.031.029562
GTGGTAT20600.029.1248041
CGCAGAG126700.028.5874988
ACGCAGA127750.028.539717
GCAGAGT137150.025.5076929
GAGTACT88050.023.15816312-13
GTACATG84500.023.047631
TACATGG82300.022.8850962
CAGAGTA132850.022.3779510-11
GTACCGT1754.0146733E-621.7377286
GTACTTT93550.021.69459714-15
TAAGGTG13600.021.6834035
GTAAGGT13750.021.100944
Produced by FastQC (version 0.11.5)