##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938100_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2515572 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.87436018527794 33.0 31.0 34.0 30.0 34.0 2 31.999980521328748 33.0 31.0 34.0 30.0 34.0 3 32.02368844938646 34.0 31.0 34.0 30.0 34.0 4 35.53958980303486 37.0 35.0 37.0 33.0 37.0 5 35.54174716525705 37.0 35.0 37.0 33.0 37.0 6 35.56308267066099 37.0 35.0 37.0 33.0 37.0 7 35.48918218202461 37.0 35.0 37.0 33.0 37.0 8 35.48251133340648 37.0 35.0 37.0 33.0 37.0 9 37.09811883738569 39.0 37.0 39.0 33.0 39.0 10-11 37.003036486333926 39.0 37.0 39.0 33.0 39.0 12-13 36.844850197092356 39.0 37.0 39.0 33.0 39.0 14-15 38.00763484408317 40.0 38.0 41.0 33.0 41.0 16-17 37.815000723493505 40.0 38.0 41.0 33.0 41.0 18-19 37.99119623687972 40.0 38.0 41.0 33.0 41.0 20-21 37.98797251678744 40.0 38.0 41.0 33.0 41.0 22-23 37.868257597079314 40.0 38.0 41.0 33.0 41.0 24-25 37.73947952990413 40.0 38.0 41.0 32.0 41.0 26-27 37.547446067932064 40.0 37.5 41.0 32.0 41.0 28-29 37.36069410853675 40.0 37.0 41.0 31.5 41.0 30-31 37.12664654400669 40.0 37.0 41.0 31.0 41.0 32-33 36.82925056408642 39.5 36.0 41.0 30.0 41.0 34-35 36.57937101382906 39.0 36.0 41.0 30.0 41.0 36-37 36.29879268015386 39.0 35.0 41.0 30.0 41.0 38-39 35.98523258328524 39.0 35.0 40.0 29.0 41.0 40-41 35.86166307305058 39.0 35.0 40.0 28.5 41.0 42-43 35.62864330657202 39.0 35.0 40.0 27.0 41.0 44-45 35.284684755594355 38.5 35.0 40.0 26.0 41.0 46-47 34.88846492964622 38.0 34.0 40.0 24.5 41.0 48-49 34.47038188531276 38.0 34.0 40.0 23.5 41.0 50-51 33.73125833806387 37.5 33.0 39.5 22.5 40.5 52-53 33.79740909820907 38.0 33.0 39.5 22.0 40.5 54-55 33.85317017362254 38.0 33.0 40.0 21.0 41.0 56-57 33.51183766554883 38.0 33.0 40.0 19.0 41.0 58-59 33.069172339332766 37.0 32.5 40.0 16.0 41.0 60-61 32.540653378237636 36.5 31.5 39.5 10.5 41.0 62-63 31.961078832170177 36.0 31.0 39.0 7.5 41.0 64-65 31.38979981491287 35.0 30.5 39.0 4.5 40.0 66-67 30.77469358857548 35.0 29.5 38.5 2.0 40.0 68-69 30.055071371441564 35.0 28.5 38.0 2.0 40.0 70-71 29.428232624627718 34.0 28.0 37.0 2.0 39.5 72-73 28.696525482077234 34.0 26.5 36.5 2.0 39.0 74-75 27.989884606761404 34.0 26.0 36.0 2.0 39.0 76-77 27.205793155592445 33.0 24.5 35.0 2.0 37.5 78-79 26.407157298618365 32.5 22.5 35.0 2.0 37.0 80-81 25.65020281669537 32.0 20.0 35.0 2.0 36.5 82-83 24.873611051482527 31.0 17.5 35.0 2.0 36.0 84-85 24.10653819489166 31.0 9.0 34.0 2.0 35.5 86-87 23.323935470739855 30.5 4.5 34.0 2.0 35.0 88-89 22.39362399486081 29.5 2.0 34.0 2.0 35.0 90-91 21.450821721660123 29.0 2.0 34.0 2.0 35.0 92-93 20.466830605524308 28.0 2.0 34.0 2.0 35.0 94-95 19.3368160402485 25.5 2.0 33.0 2.0 35.0 96-97 18.02576014520753 24.0 2.0 33.0 2.0 34.5 98-99 16.444242701063615 10.0 2.0 32.0 2.0 34.0 100-101 12.691745455904265 2.0 2.0 27.5 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2592780086523412 1101 2 0.1710531922679266 1101 3 0.23967637252618346 1101 4 0.10438294918956359 1101 5 0.06793881861290885 1101 6 0.1780269694140344 1101 7 0.2086257319102458 1101 8 0.26165904952683405 1101 9 0.2738486205857029 1101 10-11 0.4091631834214411 1101 12-13 0.3783866136557563 1101 14-15 0.442360775753464 1101 16-17 0.3961649453811944 1101 18-19 0.5020036855816201 1101 20-21 0.40581427796728775 1101 22-23 0.3358862415077297 1101 24-25 0.38259030886780465 1101 26-27 0.37659450687613116 1101 28-29 0.3414653167505435 1101 30-31 0.32256343892792216 1101 32-33 0.39268173869120204 1101 34-35 0.3419554558850919 1101 36-37 0.2375557371065824 1101 38-39 0.24118439620379206 1101 40-41 0.4059972393554574 1101 42-43 0.2868587173660089 1101 44-45 0.45360687451706383 1101 46-47 0.5292601281877509 1101 48-49 0.5967778457071304 1101 50-51 0.5419683434276621 1101 52-53 0.34855598444850244 1101 54-55 0.48395858496477473 1101 56-57 0.4291784555754887 1101 58-59 0.419823349793063 1101 60-61 0.2912907265300433 1101 62-63 0.24907809454875007 1101 64-65 0.16706574046201794 1101 66-67 0.0034563024713669677 1101 68-69 0.1519705546524719 1101 70-71 0.12745840737021297 1101 72-73 0.13242732939709967 1101 74-75 0.0011363661246974743 1101 76-77 -0.20137840257062578 1101 78-79 -0.20066301813621124 1101 80-81 -0.38456967242951734 1101 82-83 -0.482220895120161 1101 84-85 -0.5897050919128297 1101 86-87 -0.43223002337825633 1101 88-89 -0.2914845896823337 1101 90-91 -0.46475029775277577 1101 92-93 -0.40021918443282445 1101 94-95 -0.788645078851161 1101 96-97 -0.753665639449725 1101 98-99 -0.48872715814569645 1101 100-101 -0.6510052160229769 1102 1 0.20745980978827916 1102 2 0.3345291541669724 1102 3 0.3031984861472026 1102 4 0.30015510201611306 1102 5 0.26243359575506275 1102 6 0.3395520302678321 1102 7 0.34354643447326794 1102 8 0.3526597620446452 1102 9 0.2931624557043051 1102 10-11 0.36996245198270117 1102 12-13 0.3658691842427473 1102 14-15 0.33659513445334 1102 16-17 0.4254683058061133 1102 18-19 0.38127261332754614 1102 20-21 0.4322199452112443 1102 22-23 0.5053085523495966 1102 24-25 0.4597179117737866 1102 26-27 0.3683270416667668 1102 28-29 0.2225161782493501 1102 30-31 0.29409617895096574 1102 32-33 0.2581969845268972 1102 34-35 0.24563802664254553 1102 36-37 0.4018473443967707 1102 38-39 0.24418447120566356 1102 40-41 0.36254558488228383 1102 42-43 0.4352485421684449 1102 44-45 0.2619670693173859 1102 46-47 0.2959208785133285 1102 48-49 0.05415547332963655 1102 50-51 0.13931747459912458 1102 52-53 0.08058792161056516 1102 54-55 0.07723875787818457 1102 56-57 0.329983788208807 1102 58-59 0.2552041419833202 1102 60-61 0.3416145343411472 1102 62-63 0.06499036025311966 1102 64-65 0.07279594763765118 1102 66-67 0.17324307554978446 1102 68-69 -0.10534724192880418 1102 70-71 -0.36636160858472877 1102 72-73 -0.32076308417005706 1102 74-75 -0.3458300850252165 1102 76-77 -0.37371470671686424 1102 78-79 -0.515243041727711 1102 80-81 -0.5094222255615257 1102 82-83 -0.5875923504292722 1102 84-85 -0.6941018424565932 1102 86-87 -0.5999635135905059 1102 88-89 -0.5226877774324805 1102 90-91 -0.4917527838944764 1102 92-93 -0.6358098753364629 1102 94-95 -1.0709341318161236 1102 96-97 -1.0158595167398374 1102 98-99 -0.9110352300520397 1102 100-101 -1.1869939708554824 1103 1 0.2569871393159566 1103 2 0.15010682484513893 1103 3 0.24924088399149724 1103 4 0.1559518758882419 1103 5 0.2777545906452161 1103 6 0.271612697716364 1103 7 0.3614166857176144 1103 8 0.29983140607235725 1103 9 0.23438198987334857 1103 10-11 0.30979210644888866 1103 12-13 0.25476353130954266 1103 14-15 0.3380632738203815 1103 16-17 0.30948370476235 1103 18-19 0.20537631145963076 1103 20-21 0.3444415391905622 1103 22-23 0.3403017794579384 1103 24-25 0.19261638522307578 1103 26-27 0.3067506076305193 1103 28-29 0.17433825614585885 1103 30-31 0.28052145847321697 1103 32-33 0.25214354481079937 1103 34-35 0.14093146856223626 1103 36-37 0.10593045039257021 1103 38-39 0.1901736683888018 1103 40-41 0.19894748594575162 1103 42-43 0.3659210204024177 1103 44-45 0.3458147917225034 1103 46-47 0.4126947387115436 1103 48-49 0.5518839542588481 1103 50-51 0.49330950305182597 1103 52-53 0.508469304223155 1103 54-55 0.5955593750933659 1103 56-57 0.5721622468931713 1103 58-59 0.6076357502959837 1103 60-61 0.5029755206157702 1103 62-63 0.35530736260767526 1103 64-65 0.09174473041646891 1103 66-67 0.006963066647873717 1103 68-69 0.08587850727074908 1103 70-71 0.1605864653822877 1103 72-73 0.14913826887688586 1103 74-75 0.08353405470617048 1103 76-77 0.03731111539245191 1103 78-79 0.15323629245060388 1103 80-81 0.29687237722642834 1103 82-83 0.1485420159448907 1103 84-85 -0.024656443935910488 1103 86-87 -0.3073598772109598 1103 88-89 -0.20652718661278868 1103 90-91 -0.2905529548525614 1103 92-93 -0.6166350643280794 1103 94-95 -0.7193653686191261 1103 96-97 -0.98653640615386 1103 98-99 -1.240912641807057 1103 100-101 -1.1910791849896363 1104 1 0.3439540242486565 1104 2 0.24292823182839385 1104 3 0.28353350227185814 1104 4 0.14177510735547827 1104 5 0.23403344092121614 1104 6 0.3091999847445095 1104 7 0.2623535938727031 1104 8 0.3488131328000321 1104 9 0.35229069773177457 1104 10-11 0.3160906564253807 1104 12-13 0.30964436750736013 1104 14-15 0.3744643323977286 1104 16-17 0.24476584989171357 1104 18-19 0.3565038469883248 1104 20-21 0.39619811735811794 1104 22-23 0.5678832618133285 1104 24-25 0.49811775275524184 1104 26-27 0.37120518440451633 1104 28-29 0.3838765114133551 1104 30-31 0.32807777247646897 1104 32-33 0.4449355776389581 1104 34-35 0.319963989869116 1104 36-37 0.38778423836618714 1104 38-39 0.1882240073085768 1104 40-41 0.146980617629481 1104 42-43 0.12466495494402352 1104 44-45 0.13911342256562165 1104 46-47 0.13403028978435572 1104 48-49 0.20986539776870217 1104 50-51 0.030304137422071165 1104 52-53 0.12370378235463875 1104 54-55 0.07521034144952665 1104 56-57 0.0331710561856724 1104 58-59 0.020834475778912065 1104 60-61 -0.03143110483475908 1104 62-63 0.05869393867418182 1104 64-65 -0.026311955979437585 1104 66-67 0.13197797527781674 1104 68-69 0.06610218656922484 1104 70-71 0.12362737170882454 1104 72-73 0.030954928361563105 1104 74-75 -0.15883014198361423 1104 76-77 -0.18534553415190302 1104 78-79 -0.13588829126167923 1104 80-81 -0.3419646314765181 1104 82-83 -0.42534441555867986 1104 84-85 -0.3788412732833315 1104 86-87 -0.6380973549772584 1104 88-89 -0.6680486127603267 1104 90-91 -0.7797131346497146 1104 92-93 -0.71099118321753 1104 94-95 -1.0008887665829853 1104 96-97 -1.0338105177933734 1104 98-99 -1.0675889718507072 1104 100-101 -0.8485199483410284 1105 1 0.26419488679763603 1105 2 0.30363569965687986 1105 3 0.294424310993584 1105 4 0.15383534499153484 1105 5 0.20465245606582982 1105 6 0.22825186387954233 1105 7 0.2251602372345971 1105 8 0.24996845453964767 1105 9 0.33701339572196076 1105 10-11 0.3057052965998892 1105 12-13 0.3535324340597015 1105 14-15 0.30480090374668123 1105 16-17 0.37849732680466985 1105 18-19 0.35519072742059166 1105 20-21 0.44831985040358546 1105 22-23 0.41224734075623815 1105 24-25 0.3258199139813911 1105 26-27 0.45366982655193766 1105 28-29 0.3216061676627149 1105 30-31 0.21614353914728923 1105 32-33 0.2678902730060386 1105 34-35 0.30806795765168715 1105 36-37 0.2965721003295769 1105 38-39 0.3502520673171503 1105 40-41 0.40871471414700267 1105 42-43 0.3712804551673301 1105 44-45 0.45168686106026 1105 46-47 0.5428030493886666 1105 48-49 0.39851970249974045 1105 50-51 0.40718691273632857 1105 52-53 0.4439917328370555 1105 54-55 0.3586828434582259 1105 56-57 0.26496747568695156 1105 58-59 0.3527618056713493 1105 60-61 0.37486661381858966 1105 62-63 0.32607919828286214 1105 64-65 0.20325690920660122 1105 66-67 0.29563438386349716 1105 68-69 0.3307677993880347 1105 70-71 0.07462894619522942 1105 72-73 0.09789425808220287 1105 74-75 0.06396308908666981 1105 76-77 -0.17381414097411962 1105 78-79 -0.4630752263417861 1105 80-81 -0.3312202196112679 1105 82-83 -0.4731642459862506 1105 84-85 -0.5813292516309119 1105 86-87 -0.485777929432583 1105 88-89 -0.4956113608798631 1105 90-91 -0.5774249788450518 1105 92-93 -0.8487511489412825 1105 94-95 -1.194804541162906 1105 96-97 -1.4407667167618037 1105 98-99 -1.4077219459399473 1105 100-101 -1.3269772624397191 1106 1 0.23087951335157086 1106 2 0.2968031770655344 1106 3 0.2588227327350978 1106 4 0.26147203691076015 1106 5 0.22556357959748397 1106 6 0.15551897971112538 1106 7 0.3072343749782931 1106 8 0.12482525223894214 1106 9 0.38654988339794016 1106 10-11 0.2538623829309117 1106 12-13 0.4217592564400263 1106 14-15 0.40228367427579315 1106 16-17 -0.058747497271994575 1106 18-19 0.3400076027319443 1106 20-21 0.46627029490613126 1106 22-23 0.614028724261459 1106 24-25 0.42113566352277587 1106 26-27 0.257899911158475 1106 28-29 0.3076674376534996 1106 30-31 0.3474820638882363 1106 32-33 0.3251784617305944 1106 34-35 0.49245567800897305 1106 36-37 0.4807399968850987 1106 38-39 0.4159680154547587 1106 40-41 0.46482206047394925 1106 42-43 0.4957631610259412 1106 44-45 0.7042987946132726 1106 46-47 0.6495943655005121 1106 48-49 0.8494610683445103 1106 50-51 0.5663264856612642 1106 52-53 0.4868504784790204 1106 54-55 0.4453022739440442 1106 56-57 0.315839959058529 1106 58-59 0.3926331281421511 1106 60-61 0.6715816876929921 1106 62-63 0.4687810946568618 1106 64-65 0.30689092559340025 1106 66-67 0.1971901288127249 1106 68-69 0.1893684452357718 1106 70-71 0.14203834831735662 1106 72-73 0.044180279506218056 1106 74-75 0.10753824226910069 1106 76-77 -0.3774849468636461 1106 78-79 -0.26383910240476993 1106 80-81 -0.1937306353829662 1106 82-83 -0.3995568190541334 1106 84-85 -0.37371084264085397 1106 86-87 -0.45301328030547694 1106 88-89 -0.6799886444987919 1106 90-91 -0.6216040650750614 1106 92-93 -0.608317584465258 1106 94-95 -0.7220538399911369 1106 96-97 -0.7017694152177789 1106 98-99 -1.0399379021846684 1106 100-101 -1.152495684121595 1107 1 0.023406053378167258 1107 2 0.0837563835373416 1107 3 0.07656892911798963 1107 4 0.1528071167270184 1107 5 0.09045854402180709 1107 6 0.08775528403710098 1107 7 0.129453779988836 1107 8 0.1143209319902212 1107 9 0.07944367973398414 1107 10-11 0.19137474471829563 1107 12-13 0.13901189654494317 1107 14-15 0.12815418562287562 1107 16-17 0.10208819090944843 1107 18-19 0.15400895081292987 1107 20-21 0.12139443479266987 1107 22-23 0.20638489830796658 1107 24-25 0.2199390679272426 1107 26-27 -0.012185165056237679 1107 28-29 0.10218262593654259 1107 30-31 0.021108166152899344 1107 32-33 0.16770136631124188 1107 34-35 0.18180673649999335 1107 36-37 0.2041873860501866 1107 38-39 -0.09008233267521604 1107 40-41 0.040637124914567835 1107 42-43 0.008801785393529826 1107 44-45 -0.00706332139592547 1107 46-47 -0.09875150659043186 1107 48-49 -0.2076549230585485 1107 50-51 -0.2660041199294554 1107 52-53 -0.2056497744768322 1107 54-55 -0.1263514336430518 1107 56-57 -0.25733317850420434 1107 58-59 -0.3099051080208284 1107 60-61 -0.12840480437962043 1107 62-63 -0.22792729291862912 1107 64-65 -0.18192478170252357 1107 66-67 -0.22156528092576266 1107 68-69 -0.3185447087907036 1107 70-71 -0.41166761172934585 1107 72-73 -0.4711362186898711 1107 74-75 -0.4679849695872349 1107 76-77 -0.611384455002213 1107 78-79 -0.7796135481518043 1107 80-81 -0.7263291453755301 1107 82-83 -0.7146875281660847 1107 84-85 -0.842090004908691 1107 86-87 -0.8178688395463816 1107 88-89 -0.9832121240900378 1107 90-91 -1.0282483552470332 1107 92-93 -1.146104019896093 1107 94-95 -1.1344289997092467 1107 96-97 -1.3898829814648437 1107 98-99 -1.5404918627954167 1107 100-101 -1.2756379839504675 1108 1 0.03311421105335555 1108 2 0.02543680015713079 1108 3 0.02398184726722974 1108 4 0.1186734518985233 1108 5 0.07272582052867449 1108 6 0.09129210749706829 1108 7 0.06581436579122624 1108 8 0.0058078119669318085 1108 9 0.04022385182194199 1108 10-11 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2316 64-65 -0.18036552909410375 2316 66-67 -0.21815122838055956 2316 68-69 -0.2696037810903178 2316 70-71 -0.30071767226964496 2316 72-73 -0.2835900060237968 2316 74-75 -0.09608425354100802 2316 76-77 -0.27623034114133205 2316 78-79 -0.1762856187392856 2316 80-81 -0.1604583072762118 2316 82-83 -0.3825312782662742 2316 84-85 -0.36760414623683246 2316 86-87 -0.2709277543949078 2316 88-89 -0.20588506885166424 2316 90-91 -0.19614838876773177 2316 92-93 -0.22540433148538597 2316 94-95 -0.304422740850395 2316 96-97 -0.29005341098689996 2316 98-99 -0.4124806255849016 2316 100-101 -0.41393222583594635 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8531.0 3 2885.0 4 2731.0 5 3017.0 6 3527.0 7 4655.0 8 5693.0 9 7020.0 10 8707.0 11 10854.0 12 13445.0 13 16338.0 14 18819.0 15 22734.0 16 26411.0 17 32369.0 18 32013.0 19 28784.0 20 29700.0 21 31884.0 22 35397.0 23 39319.0 24 43711.0 25 48564.0 26 54964.0 27 62353.0 28 71234.0 29 81649.0 30 95007.0 31 112071.0 32 133554.0 33 164856.0 34 205982.0 35 262672.0 36 319465.0 37 318340.0 38 148128.0 39 8189.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.84846829746781 18.31928330525451 14.378720178855211 23.45352821842247 2 15.686532639493207 19.3874994233024 40.16245372498922 24.76351421221517 3 19.572090193809917 23.20965398793132 29.23599137030111 27.98226444795765 4 13.021972095148593 16.16436262375499 36.30078616483629 34.51287911626013 5 14.01804725455662 36.828594893461776 34.05797967294131 15.095378179040301 6 33.1849219446114 35.31956046967464 16.910892224892248 14.584625360821704 7 29.34181758383613 32.2848268170483 19.86050439641693 18.512851202698652 8 26.82073313841319 34.584641088370425 19.230582572548084 19.3640432006683 9 26.324434451991852 16.739440961281367 19.97231512204868 36.9638094646781 10-11 25.41545729550253 26.216427763633625 26.593823193981407 21.77429174688243 12-13 26.174328443300677 23.557216089332808 27.138009121832518 23.130446345533997 14-15 22.885858606565943 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28.559295136944456 22.798973393829865 40-41 22.867958359916567 26.004667246857345 28.62023868429301 22.507135708933077 42-43 22.491701071924574 26.036707206498573 28.5095103299305 22.962081391646358 44-45 22.51064398389225 26.37210556414109 28.5457410940894 22.571509357877265 46-47 22.999863978261974 26.57190618159141 27.842794666038795 22.585435174107822 48-49 22.689082648327282 26.59564039275161 27.780978085042623 22.934298873878486 50-51 22.83232235167684 26.872775406159278 27.734791225758414 22.560111016405465 52-53 23.050295696819006 26.55863616427963 27.747149298164903 22.643918840736458 54-55 22.67639496244504 26.801872478189885 27.840346177785996 22.681386381579077 56-57 22.781370133619202 26.942715200715096 27.645106699728185 22.63080796593752 58-59 22.720033313056224 26.566267468126192 27.6153543121536 23.098344906663986 60-61 22.508856552775352 26.769506138694105 27.881126043305592 22.84051126522495 62-63 22.535472032731573 26.745203726171756 28.03360976219932 22.68571447889735 64-65 22.37244444479794 26.70155267041575 27.91197782842481 23.014025056361504 66-67 22.33779114320971 26.782371681049927 27.793382122815718 23.086455052924645 68-69 22.78593121938316 26.390002128137763 27.949151429929575 22.874915222549507 70-71 22.51989211098894 26.5545139595279 27.638037657743332 23.287556271739827 72-73 22.56924786209549 26.661488327159695 27.545723604339067 23.223540206405747 74-75 22.489225070018403 27.043001291500257 27.396647666997744 23.071125971483593 76-77 22.68509940542032 26.646068036710147 27.474340511177893 23.194492046691643 78-79 22.800979010440802 26.82658389889258 27.346785084703168 23.025652005963444 80-81 22.868822897650933 26.844745722814267 27.133264649711542 23.15316672982326 82-83 22.799997932412968 26.856245842460208 27.214574580051202 23.129181645075626 84-85 22.78781153258006 26.903230372483268 27.14427772471975 23.164680370216924 86-87 22.92596178506035 26.778907230063005 27.018133308946172 23.27699767593047 88-89 22.832191803299228 26.94394738480332 26.80152214612022 23.42233866577723 90-91 22.828386457988437 26.862878218679164 26.858739462088987 23.449995861243412 92-93 22.95295821564781 26.505832606781272 26.96991089816157 23.571298279409344 94-95 22.77318611730114 26.35686268971472 27.101827582756805 23.768123610227335 96-97 23.053087822821276 26.026864331031057 27.067084451951178 23.852963394196486 98-99 22.798462249196213 26.318560061952322 26.633890059687182 24.249087629164286 100-101 23.72338663966322 25.099929466236144 26.76087621085615 24.415807683244488 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 626.0 1 445.0 2 368.0 3 501.0 4 431.0 5 426.5 6 779.0 7 1648.0 8 2791.5 9 3279.5 10 2794.0 11 2231.0 12 2178.5 13 2319.5 14 2478.5 15 2675.0 16 2846.5 17 3041.5 18 3377.0 19 3790.0 20 4285.0 21 5063.0 22 6056.0 23 7398.0 24 9301.0 25 11531.5 26 14707.5 27 18495.5 28 21555.0 29 24625.5 30 28039.0 31 32555.0 32 38755.5 33 42490.5 34 44334.5 35 47157.5 36 51519.0 37 56487.5 38 60165.0 39 63040.5 40 66833.5 41 71585.5 42 73903.5 43 75721.0 44 79095.5 45 83597.5 46 86233.0 47 88236.0 48 89758.5 49 89550.0 50 90454.5 51 90129.0 52 90146.5 53 91754.0 54 92857.5 55 89302.0 56 82811.0 57 76737.0 58 71594.5 59 64664.5 60 53897.0 61 44652.0 62 36742.5 63 28094.0 64 20608.5 65 15572.0 66 11841.0 67 9006.0 68 6758.5 69 4983.5 70 3799.5 71 2647.5 72 1767.5 73 1254.5 74 868.5 75 616.5 76 419.0 77 264.5 78 167.5 79 105.0 80 67.0 81 50.5 82 37.5 83 22.5 84 18.0 85 13.5 86 11.0 87 9.0 88 5.0 89 4.5 90 7.0 91 6.0 92 4.5 93 5.5 94 3.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14294959555918096 2 0.04992900223090414 3 0.1503037877667584 4 0.12915551612118437 5 0.19200404520323808 6 0.19808616092085615 7 0.16278603832448446 8 0.06845361611593705 9 0.1196149424464893 10-11 0.10168661441612484 12-13 0.15090007362142685 14-15 0.13561527954675914 16-17 0.13358790764088643 18-19 0.13277298363950626 20-21 0.13376679339728698 22-23 0.1960786652101391 24-25 0.21416600280174847 26-27 0.1475608728352836 28-29 0.12643247738486516 30-31 0.09995738543758637 32-33 0.12450448645477052 34-35 0.09379576493934581 36-37 0.08775340161203893 38-39 0.050406030914638904 40-41 0.05787948029315002 42-43 0.06131806205507137 44-45 0.03999090465309679 46-47 0.050406030914638904 48-49 0.04199840036381387 50-51 0.05362597452984848 52-53 0.026435339556967558 54-55 0.04996875462121537 56-57 0.026395587166656333 58-59 0.04939234496170254 60-61 0.03611504659775192 62-63 0.05177748838037632 64-65 0.03979214270154065 66-67 0.03812254230846901 68-69 0.06507466293948255 70-71 0.052532783796289666 72-73 0.04978986886481484 74-75 0.041918895583191416 76-77 0.02059173818121684 78-79 0.02319551974660236 80-81 0.026196825215100184 82-83 0.02238059574522216 84-85 0.020114709497482085 86-87 0.023334653112691665 88-89 0.0644585008896585 90-91 0.10892154945276859 92-93 0.07900787574356846 94-95 0.07539040822524658 96-97 0.06682376811317664 98-99 0.05487817482465221 100-101 0.13118288802705708 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2515572.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.70671466714337 #Duplication Level Percentage of deduplicated Percentage of total 1 77.1744223959511 42.99133528012848 2 12.82449287829373 14.288207310438422 3 4.353925940506964 7.27628730149086 4 1.8670774908123922 4.160350121685277 5 1.0008079499270561 2.7875861451597617 6 0.5948283284050528 1.9881559179836479 7 0.4120992238728626 1.6069685715185758 8 0.2848404639908442 1.2694021162555746 9 0.2184299577545735 1.095121379825119 >10 1.1409014188710975 11.70006971817595 >50 0.08365840796020796 3.1854364165402527 >100 0.039442572830743684 3.9275852633808976 >500 0.0027151111449270947 1.0160014486543463 >1k 0.002214959091908583 2.002150939046868 >5k 1.429005865747473E-4 0.705342069715907 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9708 0.3859162051414151 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8013 0.3185359035638813 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4790 0.1904139495907889 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2869 0.11404960780291719 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2666 0.10597987256973762 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 7.950478062245883E-5 14-15 0.0 0.0 0.0 0.0 7.950478062245883E-5 16-17 0.0 0.0 0.0 0.0 7.950478062245883E-5 18-19 0.0 0.0 0.0 3.975239031122942E-5 7.950478062245883E-5 20-21 0.0 0.0 0.0 3.975239031122942E-5 7.950478062245883E-5 22-23 0.0 0.0 0.0 7.950478062245883E-5 7.950478062245883E-5 24-25 0.0 0.0 0.0 2.583905370229912E-4 7.950478062245883E-5 26-27 0.0 0.0 0.0 6.161620498240559E-4 7.950478062245883E-5 28-29 0.0 0.0 0.0 0.0024248958089849943 7.950478062245883E-5 30-31 0.0 0.0 0.0 0.008984040210337847 7.950478062245883E-5 32-33 0.0 0.0 0.0 0.024566977212339778 7.950478062245883E-5 34-35 0.0 0.0 0.0 0.04812026847174321 7.950478062245883E-5 36-37 0.0 0.0 0.0 0.08856832561341914 7.950478062245883E-5 38-39 0.0 0.0 0.0 0.1493497303992889 1.3913336608930297E-4 40-41 0.0 1.987619515561471E-5 0.0 0.21005163040453623 1.5900956124491767E-4 42-43 0.0 3.975239031122942E-5 0.0 0.27401322641530435 1.5900956124491767E-4 44-45 0.0 3.975239031122942E-5 0.0 0.35298134976856155 1.5900956124491767E-4 46-47 0.0 3.975239031122942E-5 0.0 0.44379568543456516 1.7888575640053237E-4 48-49 0.0 3.975239031122942E-5 0.0 0.5400958509635184 2.3851434186737648E-4 50-51 0.0 3.975239031122942E-5 0.0 0.6437303325048935 2.782667321786059E-4 52-53 0.0 3.975239031122942E-5 0.0 0.7399509932532243 2.782667321786059E-4 54-55 0.0 3.975239031122942E-5 0.0 0.8454538371392272 2.782667321786059E-4 56-57 0.0 3.975239031122942E-5 0.0 0.9629022743137545 2.782667321786059E-4 58-59 0.0 3.975239031122942E-5 0.0 1.0886788372584844 2.782667321786059E-4 60-61 0.0 3.975239031122942E-5 0.0 1.225049412221157 2.782667321786059E-4 62-63 0.0 3.975239031122942E-5 0.0 1.3639045115782813 2.782667321786059E-4 64-65 0.0 3.975239031122942E-5 0.0 1.5031372586433622 2.782667321786059E-4 66-67 0.0 3.975239031122942E-5 0.0 1.6645915918924206 2.782667321786059E-4 68-69 0.0 3.975239031122942E-5 0.0 1.8402375284825876 2.782667321786059E-4 70-71 0.0 3.975239031122942E-5 0.0 2.0250265148443374 2.782667321786059E-4 72-73 0.0 3.975239031122942E-5 0.0 2.212876435260052 2.782667321786059E-4 74-75 0.0 3.975239031122942E-5 0.0 2.3983213360619375 2.782667321786059E-4 76-77 0.0 3.975239031122942E-5 0.0 2.6033244128969475 2.782667321786059E-4 78-79 0.0 3.975239031122942E-5 0.0 2.8281639324972607 2.782667321786059E-4 80-81 0.0 3.975239031122942E-5 0.0 3.0637962260670735 2.782667321786059E-4 82-83 0.0 3.975239031122942E-5 0.0 3.305808778281838 2.782667321786059E-4 84-85 0.0 3.975239031122942E-5 0.0 3.5488151402543835 2.782667321786059E-4 86-87 0.0 3.975239031122942E-5 0.0 3.8015807140483355 2.782667321786059E-4 88-89 0.0 3.975239031122942E-5 0.0 4.067961481523884 2.782667321786059E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4435 0.0 59.910126 1 GTATCAA 8255 0.0 44.300007 1 ATCAACG 10555 0.0 34.517967 3 TATCAAC 10555 0.0 34.471535 2 TCAACGC 10650 0.0 34.21006 4 CAACGCA 10960 0.0 33.28584 5 AACGCAG 11410 0.0 32.089817 6 TGGTATC 1870 0.0 31.02956 2 GTGGTAT 2060 0.0 29.124804 1 CGCAGAG 12670 0.0 28.587498 8 ACGCAGA 12775 0.0 28.53971 7 GCAGAGT 13715 0.0 25.507692 9 GAGTACT 8805 0.0 23.158163 12-13 GTACATG 8450 0.0 23.04763 1 TACATGG 8230 0.0 22.885096 2 CAGAGTA 13285 0.0 22.37795 10-11 GTACCGT 175 4.0146733E-6 21.737728 6 GTACTTT 9355 0.0 21.694597 14-15 TAAGGTG 1360 0.0 21.683403 5 GTAAGGT 1375 0.0 21.10094 4 >>END_MODULE