FastQCFastQC Report
Thu 26 May 2016
SRR938100_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938100_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2515572
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93450.37148608745843886No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81840.32533356230710153No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50380.20027254238797382No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA28570.11357257911918242No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA25980.10327671002857403No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43650.058.4365161
GTATCAA80050.043.376081
TATCAAC104700.032.843012
ATCAACG105200.032.459883
TCAACGC106800.032.015524
CAACGCA109500.031.2694635
AACGCAG113450.030.3057276
TGGTATC18800.029.5582772
ACGCAGA129500.026.366867
CGCAGAG130450.026.1748438
GTGGTAT22550.026.1197721
TAAGGTG14350.024.1585165
GCAGAGT144750.023.3593489
GTAAGGT13800.022.368334
GAGTACT91800.021.85793912-13
CAGAGTA136450.021.0396910-11
GTACTTT95900.020.92303314-15
AGTACTT96700.020.84857612-13
AGAGTAC131650.020.83280210-11
TACATGG82450.020.5073912
Produced by FastQC (version 0.11.5)