##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938100_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2515572 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.50326208114894 34.0 31.0 34.0 31.0 34.0 2 32.71689182420539 34.0 31.0 34.0 31.0 34.0 3 32.777231977458804 34.0 31.0 34.0 31.0 34.0 4 36.237638994232725 37.0 35.0 37.0 35.0 37.0 5 36.20695372662758 37.0 37.0 37.0 35.0 37.0 6 36.23624130018938 37.0 37.0 37.0 35.0 37.0 7 36.195336885606935 37.0 37.0 37.0 35.0 37.0 8 36.176512936222856 37.0 37.0 37.0 35.0 37.0 9 37.86767264065588 39.0 38.0 39.0 35.0 39.0 10-11 37.843242809190116 39.0 38.0 39.0 35.0 39.0 12-13 37.762350272621894 39.0 38.0 39.0 35.0 39.0 14-15 39.04340046717009 40.0 38.0 41.0 36.0 41.0 16-17 38.92275514276673 40.0 38.0 41.0 35.0 41.0 18-19 39.168949050156385 40.0 39.0 41.0 36.0 41.0 20-21 39.149011835081645 40.0 39.0 41.0 36.0 41.0 22-23 39.05611169149601 40.0 39.0 41.0 35.5 41.0 24-25 38.961879644073 40.0 38.0 41.0 35.0 41.0 26-27 38.79127729200357 40.0 38.0 41.0 35.0 41.0 28-29 38.6760331248718 40.0 38.0 41.0 34.5 41.0 30-31 38.43983674488347 40.0 38.0 41.0 34.0 41.0 32-33 38.235646206906424 40.0 38.0 41.0 34.0 41.0 34-35 38.08973803969833 40.0 38.0 41.0 33.5 41.0 36-37 38.09276319660101 40.0 38.0 41.0 33.5 41.0 38-39 37.94665427982184 40.0 38.0 41.0 33.0 41.0 40-41 37.7749774206423 40.0 37.0 41.0 33.0 41.0 42-43 37.547884934321104 40.0 37.0 41.0 33.0 41.0 44-45 37.28122232239825 40.0 37.0 41.0 32.0 41.0 46-47 36.89883871342184 40.0 36.0 41.0 31.0 41.0 48-49 36.50767201256812 39.0 36.0 41.0 30.0 41.0 50-51 36.12230856441398 39.0 35.0 40.5 29.5 41.0 52-53 35.59680184069468 38.5 35.0 40.0 28.0 41.0 54-55 35.27978885120362 38.0 34.5 40.0 27.5 41.0 56-57 34.929604082093455 38.0 34.0 40.0 26.5 41.0 58-59 34.48103492963032 37.5 34.0 40.0 26.0 41.0 60-61 34.00040010780848 37.0 33.0 40.0 25.0 41.0 62-63 33.525210965935386 36.5 33.0 39.0 23.0 41.0 64-65 33.024167465689715 36.0 32.0 39.0 22.0 40.0 66-67 32.46933500611392 35.0 31.5 39.0 20.5 40.0 68-69 31.87924058623645 35.0 31.0 38.0 18.5 40.0 70-71 31.25640053236401 35.0 30.5 37.5 15.0 39.5 72-73 30.665071602005426 34.0 29.5 37.0 9.5 39.0 74-75 30.065457875982084 34.0 29.0 36.0 7.5 39.0 76-77 28.72209441828737 32.5 27.0 35.0 7.0 37.0 78-79 29.40255436934423 34.0 29.0 35.0 2.0 37.0 80-81 29.08301094144791 34.0 29.0 35.0 2.0 37.0 82-83 28.510852402554967 34.0 27.5 35.0 2.0 36.5 84-85 27.91122198052769 33.0 26.5 35.0 2.0 36.0 86-87 27.262437727880577 33.0 25.5 35.0 2.0 35.5 88-89 26.503869696434847 32.0 24.5 35.0 2.0 35.0 90-91 25.94969335006114 32.0 23.5 34.0 2.0 35.0 92-93 25.117007384404026 31.5 19.5 34.0 2.0 35.0 94-95 24.276304951716746 31.0 14.0 34.0 2.0 35.0 96-97 23.210550920426847 31.0 2.0 34.0 2.0 35.0 98-99 21.751776931846912 30.0 2.0 34.0 2.0 35.0 100-101 17.386171415487212 23.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.15705242709702816 1101 2 0.14279582516959266 1101 3 0.1366620257019875 1101 4 0.11073939161784807 1101 5 0.0892350611030821 1101 6 0.0679035178478955 1101 7 0.06184341168301444 1101 8 0.05087120694333436 1101 9 -0.0012927237158422145 1101 10-11 0.11475314096240652 1101 12-13 0.19265795363199345 1101 14-15 0.23000158676453708 1101 16-17 0.2446364718811722 1101 18-19 0.23772360035751205 1101 20-21 0.2825707022104993 1101 22-23 0.332628946092008 1101 24-25 0.366968404777289 1101 26-27 0.38803814642916734 1101 28-29 0.3323601859868077 1101 30-31 0.3961780834203381 1101 32-33 0.3212619659692848 1101 34-35 0.2908484210567437 1101 36-37 0.3820236847066383 1101 38-39 0.3909691640055044 1101 40-41 0.378626666317885 1101 42-43 0.4701571088636669 1101 44-45 0.52240239912134 1101 46-47 0.5612502039452281 1101 48-49 0.485533410721942 1101 50-51 0.39047713526688455 1101 52-53 0.30334639274984454 1101 54-55 0.2276263329932462 1101 56-57 0.4959126868210788 1101 58-59 0.4865496834339993 1101 60-61 0.3708304812126926 1101 62-63 0.3114887780042608 1101 64-65 0.41657487582916275 1101 66-67 0.14683126459215856 1101 68-69 0.2199932809986933 1101 70-71 0.17204677404352609 1101 72-73 -0.006646878186487015 1101 74-75 0.20082220234130332 1101 76-77 -0.03263613037512059 1101 78-79 -0.054986558834510646 1101 80-81 0.08176563301350726 1101 82-83 0.0776013785226688 1101 84-85 0.07211937179216577 1101 86-87 -0.10353534588890057 1101 88-89 -0.2820896835807005 1101 90-91 -0.36776923509716397 1101 92-93 -0.4734019009108863 1101 94-95 -0.7001898337312831 1101 96-97 -0.7374445628869886 1101 98-99 -0.7690288246920005 1101 100-101 -1.137699288434682 1102 1 0.16279305356723484 1102 2 0.1982426658860703 1102 3 0.137975623882852 1102 4 0.062650618375784 1102 5 0.11613069088019046 1102 6 0.0723090257128618 1102 7 0.0768682901458817 1102 8 0.10398894113669144 1102 9 0.11832738326410919 1102 10-11 0.10583878458077578 1102 12-13 0.18232037274929525 1102 14-15 0.2161883508086433 1102 16-17 0.1974150143560891 1102 18-19 0.22719798210341935 1102 20-21 0.24917142972868334 1102 22-23 0.2711031181735919 1102 24-25 0.24775324400054188 1102 26-27 0.21626659361761824 1102 28-29 0.18994238548497577 1102 30-31 0.22219269685385257 1102 32-33 0.1972190921701653 1102 34-35 0.15246186480192847 1102 36-37 0.2599782685144163 1102 38-39 0.14840660136189143 1102 40-41 0.09511403872946289 1102 42-43 0.018589995822203775 1102 44-45 -0.1749433015666355 1102 46-47 -0.04488426759836983 1102 48-49 -0.22024435446082435 1102 50-51 -0.02767088945646634 1102 52-53 -0.08988737275339531 1102 54-55 0.03231527123943323 1102 56-57 -0.09728502664447802 1102 58-59 -0.1283080175539837 1102 60-61 -0.281781821310787 1102 62-63 -0.1754282954907609 1102 64-65 -0.1819756703208597 1102 66-67 -0.29753300502594726 1102 68-69 -0.24411192958611494 1102 70-71 -0.17359105724316493 1102 72-73 -0.2203940187400022 1102 74-75 -0.4413187091360271 1102 76-77 -0.47574561010074845 1102 78-79 -0.5824066690531744 1102 80-81 -0.6010098880790657 1102 82-83 -0.43725815489748143 1102 84-85 -0.5535635288030747 1102 86-87 -0.6166770806822726 1102 88-89 -0.5652532967619379 1102 90-91 -0.7008807944202395 1102 92-93 -0.6485869978678522 1102 94-95 -0.918849810173878 1102 96-97 -0.8916378708446864 1102 98-99 -1.1477076453557018 1102 100-101 -1.6339505796601443 1103 1 0.13246609125339148 1103 2 0.11243825299563781 1103 3 0.11871539349515103 1103 4 0.07055414837390117 1103 5 0.06266710906960782 1103 6 0.10803779422396076 1103 7 0.09137069093758754 1103 8 0.05632791069059806 1103 9 0.17044633079393634 1103 10-11 0.1691083918087415 1103 12-13 0.1584661906900635 1103 14-15 0.22272845227373494 1103 16-17 0.24740076453414162 1103 18-19 0.2623479075329982 1103 20-21 0.15476163587478453 1103 22-23 0.2638382944308617 1103 24-25 0.26389014809596034 1103 26-27 0.3297765186732988 1103 28-29 0.2842719308649393 1103 30-31 0.28089569854454055 1103 32-33 0.15944155128172355 1103 34-35 0.21655334766445833 1103 36-37 0.33629655603783704 1103 38-39 0.32645191161919485 1103 40-41 0.2421568874369271 1103 42-43 0.2528591569601417 1103 44-45 0.2931185018900422 1103 46-47 0.2174468004143435 1103 48-49 0.09880219820593794 1103 50-51 0.20819220326602306 1103 52-53 0.17292339617416275 1103 54-55 0.22178301204899498 1103 56-57 0.11783985171494038 1103 58-59 0.16911828361983083 1103 60-61 0.09750339250516049 1103 62-63 -0.07759655555866374 1103 64-65 0.06673930997103383 1103 66-67 -0.019788462469101376 1103 68-69 -0.04167386758576441 1103 70-71 -0.07790713241460523 1103 72-73 -0.09978334674703504 1103 74-75 -0.06957307313156846 1103 76-77 -0.11025328919356525 1103 78-79 -0.1420465990232067 1103 80-81 0.040701160534805325 1103 82-83 0.06859242622278927 1103 84-85 0.030670718779479245 1103 86-87 -0.027614002995186127 1103 88-89 -0.4364969571822144 1103 90-91 -0.4587644860409661 1103 92-93 -0.5338862729665834 1103 94-95 -0.6204330817380956 1103 96-97 -0.7516366019840568 1103 98-99 -0.6477641275529678 1103 100-101 -1.28210497414414 1104 1 0.11136562573488362 1104 2 0.10783070167764208 1104 3 0.10514143210508564 1104 4 0.06478713951955939 1104 5 0.04972087946673298 1104 6 3.5797583784358267E-4 1104 7 0.0068848235802718705 1104 8 0.01566990746110264 1104 9 0.046653375938561226 1104 10-11 0.1527901347466667 1104 12-13 0.12825687537189623 1104 14-15 0.13847152239174676 1104 16-17 0.060085506922241905 1104 18-19 0.08656740613776748 1104 20-21 0.10402244476996003 1104 22-23 0.10432786245169012 1104 24-25 0.12909388583258163 1104 26-27 0.11547008660107849 1104 28-29 0.06143803940420156 1104 30-31 -0.002740570764210304 1104 32-33 0.10299895532276793 1104 34-35 0.07026479339354097 1104 36-37 0.049433099148430415 1104 38-39 0.13791608493520613 1104 40-41 0.12085778173317152 1104 42-43 0.09964247465556753 1104 44-45 0.13217241038815786 1104 46-47 0.08677778233825251 1104 48-49 0.12495221207142748 1104 50-51 0.20083177112068284 1104 52-53 0.1414598173570809 1104 54-55 -0.17257803638124614 1104 56-57 -0.005322786575291616 1104 58-59 0.02624782124367897 1104 60-61 -0.07856752715514403 1104 62-63 -0.024364161496713166 1104 64-65 -0.053188663345203224 1104 66-67 -0.306383933273473 1104 68-69 -0.26937840383413914 1104 70-71 -0.3229080795510946 1104 72-73 -0.3222391221194947 1104 74-75 -0.25009987303246106 1104 76-77 -0.2850332968962057 1104 78-79 -0.19348755734070622 1104 80-81 -0.2950410935318821 1104 82-83 -0.24111871376398497 1104 84-85 -0.31595575828265154 1104 86-87 -0.4352589638044151 1104 88-89 -0.2925162403642112 1104 90-91 -0.4792599288928159 1104 92-93 -0.4897128794153076 1104 94-95 -0.6541705266407831 1104 96-97 -0.946107632715961 1104 98-99 -1.0444418755319482 1104 100-101 -1.674217358073765 1105 1 0.11310545241916259 1105 2 0.14074965421293228 1105 3 0.06099767301093095 1105 4 0.05227086301754014 1105 5 0.02111596381472225 1105 6 -0.03457631231376723 1105 7 0.07267087430813035 1105 8 0.07642626153861443 1105 9 0.05704676707639322 1105 10-11 0.13235201618284265 1105 12-13 0.14449680000326737 1105 14-15 0.11122756499401731 1105 16-17 0.07824596276557116 1105 18-19 0.15013323548774338 1105 20-21 0.14505057845709501 1105 22-23 0.14724117211709853 1105 24-25 0.05960217013125657 1105 26-27 0.055507522992215286 1105 28-29 0.07562919371678589 1105 30-31 0.1515333444761069 1105 32-33 0.14463685766969547 1105 34-35 0.3061996957659474 1105 36-37 0.18317767059236445 1105 38-39 0.1888394065044139 1105 40-41 0.11527086914073692 1105 42-43 0.18284713239770412 1105 44-45 0.07876235008697563 1105 46-47 0.08667372446532084 1105 48-49 0.10917784166520761 1105 50-51 0.03330758726788474 1105 52-53 -0.023397883501672823 1105 54-55 0.04384941939327547 1105 56-57 0.0030917590392434136 1105 58-59 -0.04817608050054645 1105 60-61 -0.19926671574816623 1105 62-63 -0.286544432333514 1105 64-65 -0.1222361252584463 1105 66-67 -0.21477904660402203 1105 68-69 -0.20831738366890917 1105 70-71 -0.22851903370242965 1105 72-73 -0.30120668644245185 1105 74-75 -0.36652823669447443 1105 76-77 -0.47580428891342663 1105 78-79 -0.2891610893826737 1105 80-81 -0.47337594757674495 1105 82-83 -0.39721992715560717 1105 84-85 -0.564326012955565 1105 86-87 -0.6287278656900597 1105 88-89 -1.048599610274934 1105 90-91 -1.2311482475585294 1105 92-93 -1.0282424892925661 1105 94-95 -0.9748964455363982 1105 96-97 -0.9245659943771294 1105 98-99 -1.0747281289631516 1105 100-101 -1.763894904375153 1106 1 0.14756402145660275 1106 2 0.15672121857378585 1106 3 0.17249428079141182 1106 4 0.08969104293572627 1106 5 0.10399130759808628 1106 6 0.07028607090425254 1106 7 0.10507881062763147 1106 8 0.08731662527944906 1106 9 0.13632758634544473 1106 10-11 0.1172089611147058 1106 12-13 0.046781159749436085 1106 14-15 0.1619424722533509 1106 16-17 0.20398051811671536 1106 18-19 0.25754013279266275 1106 20-21 0.30462979898556597 1106 22-23 0.29370532630051827 1106 24-25 0.3085454969646122 1106 26-27 0.3139169715205554 1106 28-29 0.29726218543952143 1106 30-31 0.24210624872779363 1106 32-33 0.2889754582633586 1106 34-35 0.35575631143734654 1106 36-37 0.2314001542714479 1106 38-39 0.21554524945647557 1106 40-41 0.128442126139646 1106 42-43 0.1182761503695815 1106 44-45 0.07859340538472992 1106 46-47 -0.02409603903632984 1106 48-49 -0.18238157664317356 1106 50-51 -0.2673488463010685 1106 52-53 -0.2654003133980041 1106 54-55 -0.4316949729937889 1106 56-57 -0.32135643103463707 1106 58-59 -0.2987984176180092 1106 60-61 -0.4467535584689415 1106 62-63 -0.30702634500000414 1106 64-65 -0.27948935090949334 1106 66-67 -0.41728895172102654 1106 68-69 -0.6743048920113104 1106 70-71 -0.8944647578173139 1106 72-73 -0.8600044748230431 1106 74-75 -0.6665086118989443 1106 76-77 -0.7996532051979273 1106 78-79 -0.7373110936412353 1106 80-81 -0.7107117576474913 1106 82-83 -0.6573142566333736 1106 84-85 -0.6968086651395176 1106 86-87 -0.6483758643243362 1106 88-89 -0.963644280446978 1106 90-91 -1.132336190665633 1106 92-93 -1.0756725263373959 1106 94-95 -1.2225180340042492 1106 96-97 -1.4625026064739437 1106 98-99 -1.511323638817391 1106 100-101 -1.991847620731999 1107 1 0.009761807003933143 1107 2 0.02258817752239395 1107 3 0.07214495595897574 1107 4 0.021073822514651397 1107 5 -0.009014623178615011 1107 6 0.04164790895860904 1107 7 0.05105049075787349 1107 8 0.034920273199475105 1107 9 0.025581225053976198 1107 10-11 0.020051795108379622 1107 12-13 0.015458699760955597 1107 14-15 -0.028095592306407013 1107 16-17 -0.04096871190599671 1107 18-19 -0.0020150309896820318 1107 20-21 0.04562656199804138 1107 22-23 0.06259375607429973 1107 24-25 0.08751029822096967 1107 26-27 0.02137831334077589 1107 28-29 0.06185307743559321 1107 30-31 -0.02496725456587967 1107 32-33 -0.07978409537506792 1107 34-35 0.009188231737532249 1107 36-37 -0.023383846851217527 1107 38-39 -0.04060369637436878 1107 40-41 -0.04150743014104563 1107 42-43 -0.007717813603747459 1107 44-45 -0.061611847317742274 1107 46-47 -0.0655316486785793 1107 48-49 -0.09978500724997019 1107 50-51 -0.2493150005534588 1107 52-53 -0.2567031233350292 1107 54-55 -0.24161470675372954 1107 56-57 -0.31017044348396183 1107 58-59 -0.13524563611203888 1107 60-61 -0.28559457829881296 1107 62-63 -0.29434338419113004 1107 64-65 -0.3847372061234893 1107 66-67 -0.37594384688713944 1107 68-69 -0.3876872651660257 1107 70-71 -0.5302481557440757 1107 72-73 -0.8349870136659625 1107 74-75 -0.835932087795431 1107 76-77 -0.835867755150904 1107 78-79 -0.9437624763733616 1107 80-81 -0.6775869977860687 1107 82-83 -0.6443920682271091 1107 84-85 -0.7606496383611123 1107 86-87 -1.0380529604555306 1107 88-89 -1.2176684818394996 1107 90-91 -1.4510297215548036 1107 92-93 -1.3434426963254253 1107 94-95 -1.4853623018381477 1107 96-97 -1.4108324961614613 1107 98-99 -1.288605542143678 1107 100-101 -1.6992740487122902 1108 1 0.015501881736305734 1108 2 -0.029820314433969486 1108 3 -0.04180723840411105 1108 4 0.039445724334548515 1108 5 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-0.28554342070032845 2316 48-49 -0.09798180044865745 2316 50-51 -0.09142694447712074 2316 52-53 0.04882821887137112 2316 54-55 0.03982115692308241 2316 56-57 0.017134923638899124 2316 58-59 -0.04761050989053217 2316 60-61 0.15086399288837526 2316 62-63 0.23664135124651864 2316 64-65 0.5165171542089055 2316 66-67 0.4307033675644547 2316 68-69 0.5590978887875337 2316 70-71 0.6311148672123146 2316 72-73 0.7345201014964466 2316 74-75 0.9481438126532105 2316 76-77 0.8684432580064758 2316 78-79 0.8979730573329867 2316 80-81 0.814760831852805 2316 82-83 1.1737442666036735 2316 84-85 1.196910940826161 2316 86-87 1.4469163383634793 2316 88-89 1.7245653145021507 2316 90-91 1.7520860407358505 2316 92-93 1.8154411844472946 2316 94-95 2.380132197288688 2316 96-97 2.782470061777513 2316 98-99 3.2976851346502016 2316 100-101 3.6143976690945117 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 5.0 4 19.0 5 46.0 6 83.0 7 212.0 8 564.0 9 1038.0 10 1909.0 11 3073.0 12 4868.0 13 7223.0 14 9993.0 15 13872.0 16 17856.0 17 24822.0 18 25352.0 19 20876.0 20 19894.0 21 20739.0 22 22331.0 23 24657.0 24 27789.0 25 31820.0 26 36345.0 27 42962.0 28 51072.0 29 61925.0 30 74562.0 31 90844.0 32 112875.0 33 144673.0 34 195624.0 35 277369.0 36 393754.0 37 483077.0 38 258065.0 39 13382.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.16174313232358 18.75488503713622 14.797875749304069 23.28549608123613 2 15.942371824741135 19.660484059496795 39.70638049512807 24.690763620633994 3 19.725453605046457 23.14871614237229 29.492090362013386 27.63373989056787 4 13.310865255534631 16.519682028201174 36.03176855812741 34.137684158136786 5 13.95950305857809 36.858887208671824 34.187063812735836 14.994545920014247 6 33.1563795202132 35.53758367010742 16.74026270366451 14.565774106014867 7 29.27874853114918 32.351369787865345 19.80165942378115 18.568222257204326 8 26.844789177173222 34.774277977334776 19.11131941363634 19.269613431855657 9 26.15448891941872 16.751975296274566 19.915232002900336 37.17830378140638 10-11 25.405394876393917 26.335243038163885 26.589857098107313 21.66950498733489 12-13 26.162513468716504 23.4763231840016 27.226713505964796 23.134449841317103 14-15 22.791436699088717 24.996998694531502 25.541010155940675 26.670554450439106 16-17 22.402420189981733 28.22035734375404 26.49694594127223 22.880276524992 18-19 22.406891951413037 26.5533644038016 28.850496030326305 22.189247614459056 20-21 22.89081767486679 25.685430589941372 29.308662204858376 22.11508953033346 22-23 22.637296805656923 25.630174767408764 29.160743560510294 22.571784866424018 24-25 22.4794914846255 25.880373900880528 29.238202895312803 22.401931719181174 26-27 22.089963367283584 26.26601749472139 29.009604582558595 22.63441455543643 28-29 22.260813320481855 26.507983109674804 28.931609210367558 22.299594359475783 30-31 22.37964732007623 26.142623958350725 28.831055042333837 22.646673679239207 32-33 22.146970947363066 26.087207998816968 28.823265642963115 22.942555410856855 34-35 22.74412300068917 26.221750537850717 28.726187818682636 22.307938642777476 36-37 22.813367168193537 25.83278458024016 28.684564796228578 22.669283455337727 38-39 22.383140055030797 26.231380196505626 28.637541515098086 22.747938233365485 40-41 22.85621603829352 25.992907223445517 28.637492809629194 22.513383928631768 42-43 22.519711833937986 26.014770856141972 28.542326079887292 22.92319123003275 44-45 22.57921856341222 26.355298119075897 28.56553102038026 22.499952297131625 46-47 22.96627065958623 26.507635746100433 27.920715368826027 22.60537822548731 48-49 22.71513821124314 26.552348461162495 27.86782540528389 22.864687922310477 50-51 22.818861182801342 26.767813498329524 27.856007588298247 22.557317730570887 52-53 23.08758069687593 26.488453218025533 27.795167639392382 22.62879844570616 54-55 22.75291055532376 26.760431514959727 27.799065783748354 22.687592145968157 56-57 22.781384280947425 26.924565604396587 27.659536823606707 22.63451329104928 58-59 22.76054366723891 26.546154358473256 27.637723500297717 23.055578473990117 60-61 22.496649252519447 26.75789763677639 27.915329200131904 22.83012391057226 62-63 22.593708468726735 26.756194465512085 27.991956414342074 22.65814065141911 64-65 22.31461380028787 26.80781862569681 27.849877933376277 23.02768964063904 66-67 22.361129664793275 26.866652830920824 27.70286065972653 23.069356844559373 68-69 22.77672620846522 26.514955701538117 27.863613227504814 22.84470486249185 70-71 22.527539156772608 26.61992584128317 27.584899643996806 23.267635357947412 72-73 22.55042017558384 26.789366378360178 27.47149450999856 23.18871893605742 74-75 22.527265321230917 27.123142577636166 27.2698537718792 23.079738329253715 76-77 22.573249623264932 26.75687765155883 27.580366834590443 23.089505890585798 78-79 22.679803625566848 26.927465401561378 27.47399040792846 22.91874056494331 80-81 22.718000239338977 26.976782926304665 27.318914418821745 22.98630241553461 82-83 22.66113882411328 27.01020891856725 27.343155984258733 22.985496273060736 84-85 22.677588892856207 27.112967267516343 27.2600252724261 22.949418567201356 86-87 22.81596300232329 27.077952999350828 27.095429868346887 23.010654129978995 88-89 22.696389037331095 27.22929708374685 26.837369901010256 23.2369439779118 90-91 22.64910771765872 27.15664556395767 27.053696791410044 23.140549926973573 92-93 22.758221285071016 26.81319175069251 27.14856614474122 23.280020819495252 94-95 22.51115864872059 26.776403401476976 27.21006646982359 23.50237147997884 96-97 22.773147582143316 26.507036737744727 27.183664868593553 23.536150811518404 98-99 22.498003205208484 26.769593536467696 26.84493303133924 23.88747022698458 100-101 23.123814385466282 26.185624472853142 26.824591566167104 23.86596957551347 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 671.0 1 465.0 2 366.0 3 474.0 4 383.0 5 363.5 6 738.5 7 1711.0 8 2804.5 9 3309.0 10 2861.0 11 2125.0 12 1982.5 13 2133.5 14 2349.5 15 2572.0 16 2716.0 17 2861.5 18 3163.5 19 3634.0 20 4074.0 21 4762.5 22 5850.5 23 7150.0 24 8871.0 25 11237.0 26 14560.0 27 18392.0 28 21497.0 29 24679.5 30 28218.5 31 32773.0 32 39434.5 33 43163.5 34 44625.5 35 47789.5 36 52375.5 37 56803.5 38 60132.5 39 63364.0 40 67029.5 41 72007.0 42 74950.0 43 76538.0 44 80118.0 45 84839.0 46 87266.5 47 89894.0 48 91855.5 49 90928.0 50 91340.5 51 91025.5 52 90870.0 53 92680.5 54 94456.5 55 90216.5 56 82630.5 57 76476.0 58 71461.5 59 64001.0 60 52317.0 61 43003.5 62 35548.0 63 26495.5 64 18550.5 65 13902.0 66 10531.5 67 7918.0 68 5872.0 69 4347.0 70 3129.0 71 2042.5 72 1376.5 73 956.5 74 658.5 75 458.5 76 314.5 77 186.5 78 110.0 79 68.5 80 37.0 81 25.5 82 17.0 83 8.0 84 3.5 85 2.5 86 2.0 87 2.0 88 1.0 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0094610688940726 2 0.013674822267062918 3 0.00886478303940416 4 1.1925717093368824E-4 5 9.540573674695059E-4 6 0.0015105908318267178 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 8.14924001380203E-4 14-15 0.0 16-17 0.0026634101508523707 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0036770961037887206 26-27 0.01870349964143344 28-29 0.0066585253771309265 30-31 0.003577715128010647 32-33 0.0 34-35 0.009401440308605756 36-37 8.14924001380203E-4 38-39 0.0028422959072529033 40-41 0.002186381467117618 42-43 0.003100686444275895 44-45 0.0 46-47 9.938097577807354E-5 48-49 8.14924001380203E-4 50-51 0.00477028683734753 52-53 0.008646144892692397 54-55 0.008010106647712727 56-57 0.017471175541785327 58-59 0.024427843846250477 60-61 0.003518086542543803 62-63 0.020850128718239828 64-65 0.02194331945179864 66-67 0.013833831828307837 68-69 0.032278940932718284 70-71 0.016934518272583732 72-73 0.005386448887171586 74-75 0.016954394467739345 76-77 0.021545795548686342 78-79 0.027131006387414076 80-81 0.012362993386792347 82-83 0.03609517040259631 84-85 0.0229768815998906 86-87 0.0325770838600525 88-89 0.02242034813553339 90-91 0.028979492536886243 92-93 0.02470611057842908 94-95 0.014728260610310498 96-97 0.015086032123111564 98-99 0.01156794558056776 100-101 0.010435002456697722 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2515572.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.219243280194185 #Duplication Level Percentage of deduplicated Percentage of total 1 75.11942791587444 39.977991093239325 2 13.788412240102735 14.676177309072697 3 4.776308714354538 7.625746063516373 4 2.131956511510635 4.538444489955144 5 1.101338282074563 2.9306194983758647 6 0.6864603036034935 2.1919738739802175 7 0.44828572174456993 1.6700198819193193 8 0.3088111244604923 1.3147755488234614 9 0.2342264932166612 1.121882105264782 >10 1.260437550837928 12.396733171053212 >50 0.09552942659871913 3.4854607223043974 >100 0.04342443566244953 4.233456842309899 >500 0.0026906399793439465 0.9492954986095629 >1k 0.002466419981062643 1.9897975076637333 >5k 2.242199982784221E-4 0.8976263939119933 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9345 0.37148608745843886 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8184 0.32533356230710153 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5038 0.20027254238797382 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2857 0.11357257911918242 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2598 0.10327671002857403 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 5.9628585466844126E-5 14-15 0.0 0.0 0.0 0.0 7.950478062245883E-5 16-17 0.0 0.0 0.0 0.0 1.3913336608930297E-4 18-19 0.0 0.0 0.0 3.975239031122942E-5 1.5900956124491767E-4 20-21 0.0 0.0 0.0 3.975239031122942E-5 2.3851434186737648E-4 22-23 0.0 0.0 0.0 7.950478062245883E-5 2.3851434186737648E-4 24-25 0.0 0.0 0.0 2.9814292733422064E-4 2.3851434186737648E-4 26-27 0.0 0.0 0.0 6.559144401352853E-4 2.3851434186737648E-4 28-29 0.0 0.0 0.0 0.0024248958089849943 2.3851434186737648E-4 30-31 0.0 0.0 0.0 0.008944287820026618 2.3851434186737648E-4 32-33 0.0 0.0 0.0 0.024686234383273466 2.9814292733422064E-4 34-35 0.0 0.0 0.0 0.04849791617969988 3.5777151280106474E-4 36-37 0.0 0.0 0.0 0.08916461146808757 3.5777151280106474E-4 38-39 0.0 0.0 0.0 0.1501447782055135 3.5777151280106474E-4 40-41 0.0 0.0 0.0 0.21104544016231697 3.5777151280106474E-4 42-43 0.0 0.0 0.0 0.27522567431979683 3.5777151280106474E-4 44-45 0.0 0.0 0.0 0.35506835025990113 3.5777151280106474E-4 46-47 0.0 0.0 0.0 0.44630008602417265 3.5777151280106474E-4 48-49 0.0 0.0 0.0 0.5428188896998376 3.7764770795667944E-4 50-51 0.0 0.0 0.0 0.6470894094861923 4.3727629342352355E-4 52-53 0.0 0.0 0.0 0.7436280893570131 4.3727629342352355E-4 54-55 0.0 0.0 0.0 0.8499259810492406 4.3727629342352355E-4 56-57 0.0 0.0 0.0 0.9684676089573265 4.3727629342352355E-4 58-59 0.0 0.0 0.0 1.0954168674162377 4.5715248857913825E-4 60-61 0.0 0.0 0.0 1.2333179094058926 4.7702868373475295E-4 62-63 0.0 0.0 0.0 1.3729084279837747 4.7702868373475295E-4 64-65 0.0 0.0 0.0 1.5133337467581924 4.7702868373475295E-4 66-67 0.0 0.0 0.0 1.6762390422536106 4.7702868373475295E-4 68-69 0.0 0.0 0.0 1.853475074456227 4.7702868373475295E-4 70-71 0.0 0.0 0.0 2.0400926707722937 4.7702868373475295E-4 72-73 0.0 0.0 0.0 2.2300892202648144 4.7702868373475295E-4 74-75 0.0 0.0 0.0 2.4182969121933304 4.7702868373475295E-4 76-77 0.0 0.0 0.0 2.626599437424172 4.7702868373475295E-4 78-79 0.0 0.0 0.0 2.855652710397476 4.7702868373475295E-4 80-81 0.0 0.0 0.0 3.096790710025394 4.7702868373475295E-4 82-83 0.0 0.0 0.0 3.3444282254691977 4.7702868373475295E-4 84-85 0.0 0.0 0.0 3.59397385564794 4.7702868373475295E-4 86-87 0.0 0.0 0.0 3.8540141168688473 5.366572692015971E-4 88-89 0.0 0.0 0.0 4.129776448457846 5.962858546684412E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4365 0.0 58.436516 1 GTATCAA 8005 0.0 43.37608 1 TATCAAC 10470 0.0 32.84301 2 ATCAACG 10520 0.0 32.45988 3 TCAACGC 10680 0.0 32.01552 4 CAACGCA 10950 0.0 31.269463 5 AACGCAG 11345 0.0 30.305727 6 TGGTATC 1880 0.0 29.558277 2 ACGCAGA 12950 0.0 26.36686 7 CGCAGAG 13045 0.0 26.174843 8 GTGGTAT 2255 0.0 26.119772 1 TAAGGTG 1435 0.0 24.158516 5 GCAGAGT 14475 0.0 23.359348 9 GTAAGGT 1380 0.0 22.36833 4 GAGTACT 9180 0.0 21.857939 12-13 CAGAGTA 13645 0.0 21.03969 10-11 GTACTTT 9590 0.0 20.923033 14-15 AGTACTT 9670 0.0 20.848576 12-13 AGAGTAC 13165 0.0 20.832802 10-11 TACATGG 8245 0.0 20.507391 2 >>END_MODULE