FastQCFastQC Report
Thu 26 May 2016
SRR938099_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938099_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences523806
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44380.847260245205286No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41630.7947598920210918No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39060.7456959255907721No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26180.4998033623135283No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15150.28922921845110594No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10130.19339221009304972No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8120.15501922467478418No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5940.11340076287785937No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5840.11149165912570685No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26800.038.449051
GTATCAA36600.028.4358832
CTACGTC350.00840272327.13524650-51
GTATAGC2651.8189894E-1225.2028031
GTGTAGG2151.5752448E-924.4073661
GTCCTAC1601.7879574E-623.8526521
ATCAACG43750.023.6800214
TATCAAC44000.023.5454773
TCAACGC44300.023.3860265
CAACGCA44700.023.2763986
AACGCAG45300.022.9637177
ATAGGAC1302.9289504E-421.9336853
GAGTACT32100.021.52844812-13
ACGCAGA49850.020.6771568
CGCAGAG49750.020.5277619
GTACTTT34300.020.21878214-15
TAGCCCT4300.019.8933414
GTGTAGC4150.019.5419311
AGTACTT32000.018.92408812-13
GTATATT2301.9704985E-618.6672941
Produced by FastQC (version 0.11.5)