FastQCFastQC Report
Thu 26 May 2016
SRR938099_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938099_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences523806
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47080.8988060465134038No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43970.8394329198214607No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38210.7294685436974758No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26820.5120216263273043No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT26160.4994215415630978No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT16620.3172930436077479TruSeq Adapter, Index 3 (95% over 21bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13700.26154721404489445No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10200.19472858271955648No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9130.17430117257152458No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT8860.16914659244071278RNA PCR Primer, Index 3 (95% over 23bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6440.12294628163862192No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5500.10500070636838829No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27400.033.3216741
AATTGCG300.003945936431.67462534-35
GTATCAA38500.028.9022241
AACACGA350.008396585527.13931372-73
ATCAACG45050.024.381023
TCAACGC45300.024.2464644
CAACGCA45400.024.1907445
TATCAAC45550.024.1156922
AACGCAG45500.024.1122366
GTCTTAC1401.7618624E-523.7764031
CTCCGAC901.953158E-723.74689794-95
GAGTACT30350.022.53412212-13
GTATTGG2405.833499E-921.7950361
CGCAGAG50300.021.6224258
ACGCAGA51000.021.5118947
GTACTTT31050.021.4142714-15
GTATAGC2901.03682396E-1021.3167741
AGTACTT29950.020.7735812-13
GCAGAGT51700.020.761319
GTAGCCC2751.2387318E-920.7483333
Produced by FastQC (version 0.11.5)