FastQCFastQC Report
Thu 26 May 2016
SRR938098_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938098_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences515847
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44600.8645974484682474No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40660.7882182119892139No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38500.7463453310768503No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25630.4968527489740175No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14570.28244809022830414No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10450.20257944700657363No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8490.16458368469720672No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5620.10894703274420517No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5460.10584533786180784No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTG405.381496E-447.5069857
ACCGTGC554.9551854E-543.1881688
GGTATCA26450.038.0825081
GTACTAG1351.137596E-831.8256651
ACAACCG700.00836548927.1573855
TCAGCGA350.00839707127.1389586-87
GTATCAA37850.027.116981
GCTGTCG908.933158E-426.3927678
CGACGAC951.1772499E-825.02673594-95
ATCAACG43600.023.4357033
TATCAAC43850.023.1937072
TCAACGC44300.023.0653864
AACGCAG44600.022.9080146
CAACGCA44750.022.8334455
GTACATG21050.022.6786211
TAGCCCT5400.022.0025124
GACCTAC1100.00279285621.6993181
GACGACC550.002859376121.61399894-95
GAGTACT30600.021.57892212-13
TACATGG20900.021.375072
Produced by FastQC (version 0.11.5)