FastQCFastQC Report
Thu 26 May 2016
SRR938098_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938098_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences515847
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46120.8940635498510217No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44030.8535476604497071No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36520.7079618569071837No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27760.5381440620959316No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT25860.5013114353674636No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT16000.31016948823973TruSeq Adapter, Index 3 (95% over 21bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14470.2805095309268058No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10910.21149681979346588No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT8670.1680730914399037RNA PCR Primer, Index 3 (95% over 23bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8550.16574682027810572No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6270.12154766820394419No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5300.10274364297941055No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA250.00502040156.9980243
GTAGCGC250.00502040156.9980243
GGTATCA27250.034.871321
GCCGCTA350.008392598527.14191666-67
GTATCAA39600.026.995551
ATCAACG44200.024.1790263
TATCAAC44650.023.9423032
TCAACGC44850.023.8286064
CAACGCA45250.023.6179645
AACGCAG46350.022.9549756
GAGTACT32350.022.02396412-13
GTCTAGA1754.033118E-621.719851
GTACTTT32700.021.42509714-15
ACGCAGA51050.020.8415897
CGCAGAG50600.020.7453298
ATTAGGG1856.5425465E-620.5398273
TGTATAC2351.1292468E-720.2120653
GTATAAT1456.129157E-419.6602061
GTATAGA1707.9273916E-519.5638331
GCAGAGT53300.019.2488639
Produced by FastQC (version 0.11.5)